Origin IGS: ggtgttctcctcttggtagtgaattggaactgcggtctattgtactcaatttcgcaataaaatcttaataacgccggatgtgttttttttcattctaattatgaaaatttttcactcggcagtatttgtcggtgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacaccgacaaatactgccgagtgaaaaattttcataattagaatgaaaaaaaacacatccggcgttattaagattttattgcgaaattgagtacaatagaccgcagttccaattcactaccaagaggagaacacc Mask Tandem Repeat Region ================================================ ggtgttctcctcttggtagtgaattggaactgcggtctattgtactcaatttcgcaataaaatcttaataacgccggatgtgttttttttcattctaattatgaaaatttttcactcggcagtatttgtcggtgtta Find is-nt database================================================ Query_seq: AA02810:AA02811|AA02810:AA02811:glycine hydroxymethyltransferase; serine hydroxymethyltransferase:hypothetical protein:->->:1979585..1979721 137 ggtgttctcctcttggtagtgaattggaactgcggtctattgtactcaatttcgcaataaaatcttaataacgccggatgtgttttttttcattctaattatgaaaatttttcactcggcagtatttgtcggtgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02810:AA02811|AA02810:AA02811:glycine hydroxymethyltransferase; serine hydroxymethyltransferase:hypothetical protein:->->:1979585..1979721 137 ggtgttctcctcttggtagtgaattggaactgcggtctattgtactcaatttcgcaataaaatcttaataacgccggatgtgttttttttcattctaattatgaaaatttttcactcggcagtatttgtcggtgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02810:AA02811|AA02810:AA02811:glycine hydroxymethyltransferase; serine hydroxymethyltransferase:hypothetical protein:->->:1979585..1979721 137 ggtgttctcctcttggtagtgaattggaactgcggtctattgtactcaatttcgcaataaaatcttaataacgccggatgtgttttttttcattctaattatgaaaatttttcactcggcagtatttgtcggtgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ggtgttctcctcttggtagtgaattggaactgcggtctattgtactcaatttcgcaataaaatcttaataacgccggatgtgttttttttcattctaattatgaaaatttttcactcggcagtatttgtcggtgtta Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 23 End: 136 ...................... M G T A V Y C T Q F R N K I L I T P D V F F F I L I M K I F H S A V F V G V . ggtgttctcctcttggtagtgaattggaactgcggtctattgtactcaatttcgcaataaaatcttaataacgccggatgtgttttttttcattctaattatgaaaatttttcactcggcagtatttgtcggtgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ggtgttctcctcttggtagtgaattggaactgcggtctattgtactcaatttcgcaataaaatcttaataacgccggatgtgttttttttcattctaattatgaaaatttttcactcggcagtatttgtcggtgtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02810:AA02811|AA02810:AA02811:glycine hydroxymethyltransferase; serine hydroxymethyltransferase:hypothetical protein:->->:1979585..1979721 137 ggtgttctcctcttggtagtgaattggaactgcggtctattgtactcaatttcgcaataaaatcttaataacgccggatgtgttttttttcattctaattatgaaaatttttcactcggcagtatttgtcggtgtta Intra-Species Hit: Count: 1 Min: 1 Max: 137 Len: 137 Subject: aact_AA02810_AA02811|glycine hydroxymethyltransferase; serine hydroxymethyltransferase:hypothetical protein|POSITIVE:POSITIVE|[1979585,1979721]|137 HSP 1 e-value: 6.0E-58 bit: 224.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 1 Subject End: 137 ggtgttctcctcttggtagtgaattggaactgcggtctattgtactcaatttcgcaataaaatcttaataacgccggatgtgnnnnnnnncattctaattatgaaaatttttcactcggcagtatttgtcggtgtta ggtgttctcctcttggtagtgaattggaactgcggtctattgtactcaatttcgcaataaaatcttaataacgccggatgtgttttttttcattctaattatgaaaatttttcactcggcagtatttgtcggtgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUGUUCUCCUCUUGGUAGUGAAUUGGAACUGCGGUCUAUUGUACUCAAUUUCGCAAUAAAAUCUUAAUAACGCCGGAUGUGUUUUUUUUCAUUCUAAUUAUGAAAAUUUUUCACUCGGCAGUAUUUGUCGGUGUUA ((((((.(((((........))...)))..(((((...((((....)))).))))).............))))))...........(((((((.......))))))).....(((.((((((...)))))))))... (-26.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACACCGACAAAUACUGCCGAGUGAAAAAUUUUCAUAAUUAGAAUGAAAAAAAACACAUCCGGCGUUAUUAAGAUUUUAUUGCGAAAUUGAGUACAAUAGACCGCAGUUCCAAUUCACUACCAAGAGGAGAACACC .....((....((((.(((((..((.....(((((((.......)))))))......))..))))).))))..((....((((((..((((....))))....))))))......))..........))........ (-23.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 296 36 1 202 161 aact:AA02899|5end_oligopeptide_ABC_transporter,_ATP-binding_protein_2030063..2030293_POSITIVE 288 39 1 179 140 aact:AA00459|5end_hypothetical_protein_320851..321081_POSITIVE 283 50 2 224 161 aact:AA00042|5end_hypothetical_protein_33810..34040_POSITIVE 279 46 1 202 151 aact:AA00296|5end_conserved_hypothetical_protein_(possible_hemolysin_/_transport_protein)_202384..202614_POSITIVE 279 46 1 85 34 aact:AA00295|5end_hypothetical_protein_202267..202497_POSITIVE 276 117 71 89 47 aact:AA00933|5end_ribose_ABC_transporter,_ATP-binding_protein_644278..644508_POSITIVE 276 60 12 124 75 aact:AA00528|5end_conserved_hypothetical_protein_361396..361626_POSITIVE 259 44 2 135 92 aact:AA02069|5end_alkylphosphonate_uptake_protein_1406131..1406361_POSITIVE 258 46 18 173 139 aact:AA01584|5end_host_factor-I_protein_1065589..1065819_POSITIVE 254 47 3 202 163 aact:AA01320|5end_hypothetical_protein_893346..893576_POSITIVE 250 78 31 188 145 aact:AA01014|5end_UTP-glucose-1-phosphate_uridylyltransferase_709469..709699_POSITIVE 247 47 8 142 99 aact:AA00101|5end_fimbrial_biogenesis_and_twitching_motility_protein_73782..74012_POSITIVE 245 80 31 221 157 aact:AA00377|5end_acetyl-CoA_acetyltransferase_(Beta-ketothiolase,3-ketoacyl-CoA_thiolase)_253042..253272_POSITIVE 243 80 31 228 183 aact:AA01274|5end_hypothetical_protein_864519..864749_POSITIVE 243 80 31 71 26 aact:AA01273|5end_hypothetical_protein_864362..864592_POSITIVE 242 43 1 81 26 aact:AA01211|5end_ATP-dependent_Clp_protease,_ATPase_subunit_827035..827265_POSITIVE 242 38 5 136 99 aact:AA00711|5end_conserved_hypothetical_protein_487893..488123_POSITIVE 241 56 11 66 34 aact:AA02837|5end_conserved_hypothetical_protein_1992496..1992726_POSITIVE 240 80 31 213 157 aact:AA01337|5end_cell_division_protein_N_904235..904465_POSITIVE 239 82 44 132 93 aact:AA01221|5end_transformation_locus_protein_orfG_homolog_833578..833808_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 288 39 1 132 93 aact:AA00458|3end_nickel-binding_periplasmic_protein_320884..321034_POSITIVE 283 50 2 151 88 aact:AA00041|3end_cytochrome_c-type_biogenesis_protein_33817..33967_POSITIVE 279 46 1 76 25 aact:AA00292|3end_dihydrofolate_reductase_202338..202488_POSITIVE 276 60 12 111 62 aact:AA00527|3end_ATP-dependent_DNA_helicase_361463..361613_POSITIVE 259 44 2 49 6 aact:AA02067|3end_conserved_hypothetical_protein;_possible_membrane_protein_1406125..1406275_POSITIVE 255 75 37 127 93 aact:AA01421|3end_phosphoglycolate_phosphatase_955806..955956_POSITIVE 252 55 13 47 5 aact:AA02545|3end_conserved_hypothetical_protein_1774221..1774371_POSITIVE 250 78 31 109 66 aact:AA01013|3end_phosphomannomutase_709470..709620_POSITIVE 248 38 1 80 44 aact:AA00943|3end_conserved_hypothetical_protein_654542..654692_POSITIVE 247 47 8 117 74 aact:AA00099|3end_conserved_hypothetical_protein_73837..73987_POSITIVE 243 43 2 107 64 aact:AA00199|3end_hypothetical_protein_138744..138894_POSITIVE 242 43 1 65 10 aact:AA01210|3end_hypothetical_protein_827099..827249_POSITIVE 241 56 11 33 1 aact:AA02836|3end_colicin_transport_protein_1992543..1992693_POSITIVE 239 41 10 66 34 aact:AA01873|3end_mannose-6-phosphate_isomerase_1261940..1262090_POSITIVE 236 59 14 149 94 aact:AA02481|3end_conserved_hypothetical_protein_1720103..1720253_POSITIVE 234 36 6 66 33 aact:AA02799|3end_hypothetical_protein_1966115..1966265_POSITIVE 234 47 7 121 75 aact:AA01223|3end_branched-chain_amino_acid_carrier_protein_836154..836304_POSITIVE 232 46 13 71 33 aact:AA01479|3end_spermidine/putrescine-binding_periplasmic_protein_995828..995978_POSITIVE 231 79 31 117 59 aact:AA01932|3end_tRNA_pseudouridine_synthase_B_1306092..1306242_POSITIVE 229 36 9 150 110 aact:AA01245|3end_tyrosine-specific_transport_protein_2_849681..849831_POSITIVE