Origin IGS: taaagcattttctgatgctaaaaaagacctgaaaccgattcaggtcttttgtttttcggtgattagaaaacacttgaatttttgaccgcacttttgccccgaaaacctacaacgggctttccacttgcaggcgttcgcaaatgccggtaatgcgctttcaattataaatccatcatcaagtcacagttttatactgcctaaccaaatctattttcaatttagacttttgatacattgtggaaaaccggaatgttatattctatcttttataatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taattataaaagatagaatataacattccggttttccacaatgtatcaaaagtctaaattgaaaatagatttggttaggcagtataaaactgtgacttgatgatggatttataattgaaagcgcattaccggcatttgcgaacgcctgcaagtggaaagcccgttgtaggttttcggggcaaaagtgcggtcaaaaattcaagtgttttctaatcaccgaaaaacaaaagacctgaatcggtttcaggtcttttttagcatcagaaaatgcttta Mask Tandem Repeat Region ================================================ taaagcattttctgatgctaaaaaagacctgaaaccgattcaggtcttttgtttttcggtgattagaaaacacttgaatttttgaccgcacttttgccccgaaaacctacaacgggctttccacttgcaggcgttcgcaaatgccggtaatgcgctttcaattataaatccatcatcaagtcacagttttatactgcctaaccaaatctattttcaatttagacttttgatacattgtggaaaaccggaatgttatattctatcttttataatta Find is-nt database================================================ Query_seq: AA02802:AA02803|AA02802:AA02803:probable myo-inositol 2-dehydrogenase:leukotoxin secretion protein D; hemolysin export system membrane fusion protein:->->:1970147..1970421 275 taaagcattttctgatgctaaaaaagacctgaaaccgattcaggtcttttgtttttcggtgattagaaaacacttgaatttttgaccgcacttttgccccgaaaacctacaacgggctttccacttgcaggcgttcgcaaatgccggtaatgcgctttcaattataaatccatcatcaagtcacagttttatactgcctaaccaaatctattttcaatttagacttttgatacattgtggaaaaccggaatgttatattctatcttttataatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02802:AA02803|AA02802:AA02803:probable myo-inositol 2-dehydrogenase:leukotoxin secretion protein D; hemolysin export system membrane fusion protein:->->:1970147..1970421 275 taaagcattttctgatgctaaaaaagacctgaaaccgattcaggtcttttgtttttcggtgattagaaaacacttgaatttttgaccgcacttttgccccgaaaacctacaacgggctttccacttgcaggcgttcgcaaatgccggtaatgcgctttcaattataaatccatcatcaagtcacagttttatactgcctaaccaaatctattttcaatttagacttttgatacattgtggaaaaccggaatgttatattctatcttttataatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02802:AA02803|AA02802:AA02803:probable myo-inositol 2-dehydrogenase:leukotoxin secretion protein D; hemolysin export system membrane fusion protein:->->:1970147..1970421 275 taaagcattttctgatgctaaaaaagacctgaaaccgattcaggtcttttgtttttcggtgattagaaaacacttgaatttttgaccgcacttttgccccgaaaacctacaacgggctttccacttgcaggcgttcgcaaatgccggtaatgcgctttcaattataaatccatcatcaagtcacagttttatactgcctaaccaaatctattttcaatttagacttttgatacattgtggaaaaccggaatgttatattctatcttttataatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaagcattttctgatgctaaaaaagacctgaaaccgattcaggtcttttgtttttcggtgattagaaaacacttgaatttttgaccgcacttttgccccgaaaacctacaacgggctttccacttgcaggcgttcgcaaatgccggtaatgcgctttcaattataaatccatcatcaagtcacagttttatactgcctaaccaaatctattttcaatttagacttttgatacattgtggaaaaccggaatgttatattctatcttttataatta Predict ORF larger than 30AA ================================================ Protein_Len: 50 Strand: + Start: 125 End: 274 ............................................................................................................................ M Q A F A N A G N A L S I I N P S S S H S F I L P N Q I Y F Q F R L L I H C G K P E C Y I L S F I I . Protein_Len: 32 Strand: - Start: 66 End: 161 ................................................................. F V S S N K V A S K G R F G V V P K G S A P T R L H R Y H A K M .................................................................................................................. Protein_Len: 41 Strand: - Start: 43 End: 165 .......................................... T K Q K E T I L F C K F K Q G C K Q G S F R C R A K W K C A N A F A P L A S E I M .............................................................................................................. Protein_Len: 30 Strand: - Start: 2 End: 91 . F C K R I S F F V Q F R N L D K T K R H N S F V Q I K S R M ........................................................................................................................................................................................ taaagcattttctgatgctaaaaaagacctgaaaccgattcaggtcttttgtttttcggtgattagaaaacacttgaatttttgaccgcacttttgccccgaaaacctacaacgggctttccacttgcaggcgttcgcaaatgccggtaatgcgctttcaattataaatccatcatcaagtcacagttttatactgcctaaccaaatctattttcaatttagacttttgatacattgtggaaaaccggaatgttatattctatcttttataatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaagcattttctgatgctaaaaaagacctgaaaccgattcaggtcttttgtttttcggtgattagaaaacacttgaatttttgaccgcacttttgccccgaaaacctacaacgggctttccacttgcaggcgttcgcaaatgccggtaatgcgctttcaattataaatccatcatcaagtcacagttttatactgcctaaccaaatctattttcaatttagacttttgatacattgtggaaaaccggaatgttatattctatcttttataatta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 93 HP_score: -9.8 Tail_Score: -5.0538 Start: 26 End: 46 Full_Region: caccgaaaaacaaaa gacctgaa tcggt ttcaggtc ttttttagcatcaga .........................gacctgaatcggtttcaggtc..................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: AA02802:AA02803|AA02802:AA02803:probable myo-inositol 2-dehydrogenase:leukotoxin secretion protein D; hemolysin export system membrane fusion protein:->->:1970147..1970421 275 taaagcattttctgatgctaaaaaagacctgaaaccgattcaggtcttttgtttttcggtgattagaaaacacttgaatttttgaccgcacttttgccccgaaaacctacaacgggctttccacttgcaggcgttcgcaaatgccggtaatgcgctttcaattataaatccatcatcaagtcacagttttatactgcctaaccaaatctattttcaatttagacttttgatacattgtggaaaaccggaatgttatattctatcttttataatta Intra-Species Hit: Count: 1 Min: 1 Max: 275 Len: 275 Subject: aact_AA02802_AA02803|probable myo-inositol 2-dehydrogenase:leukotoxin secretion protein D; hemolysin export system membrane fusion protein|POSITIVE:POSITIVE|[1970147,1970421]|275 HSP 1 e-value: 1.0E-154 bit: 545.0 Len: 275 Query Start:1 Query End:275 Subject Strand: POSITIVE Subject Start: 1 Subject End: 275 taaagcattttctgatgctaaaaaagacctgaaaccgattcaggtcttttgtttttcggtgattagaaaacacttgaatttttgaccgcacttttgccccgaaaacctacaacgggctttccacttgcaggcgttcgcaaatgccggtaatgcgctttcaattataaatccatcatcaagtcacagttttatactgcctaaccaaatctattttcaatttagacttttgatacattgtggaaaaccggaatgttatattctatcttttataatta taaagcattttctgatgctaaaaaagacctgaaaccgattcaggtcttttgtttttcggtgattagaaaacacttgaatttttgaccgcacttttgccccgaaaacctacaacgggctttccacttgcaggcgttcgcaaatgccggtaatgcgctttcaattataaatccatcatcaagtcacagttttatactgcctaaccaaatctattttcaatttagacttttgatacattgtggaaaaccggaatgttatattctatcttttataatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAGCAUUUUCUGAUGCUAAAAAAGACCUGAAACCGAUUCAGGUCUUUUGUUUUUCGGUGAUUAGAAAACACUUGAAUUUUUGACCGCACUUUUGCCCCGAAAACCUACAACGGGCUUUCCACUUGCAGGCGUUCGCAAAUGCCGGUAAUGCGCUUUCAAUUAUAAAUCCAUCAUCAAGUCACAGUUUUAUACUGCCUAACCAAAUCUAUUUUCAAUUUAGACUUUUGAUACAUUGUGGAAAACCGGAAUGUUAUAUUCUAUCUUUUAUAAUUA .(((((.((((((((((((..((((((((((((.....)))))))))))).......))).)))))))))..............((((((..(((((...(((..(((.(((..((....))..))).)))..)))))))))).)))).....))))).(((((((((...((((..(((((.((((.....)))).......(((.....))).......))))).))))(((((.(((....))).)))))............))))))))). (-60.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUAUAAAAGAUAGAAUAUAACAUUCCGGUUUUCCACAAUGUAUCAAAAGUCUAAAUUGAAAAUAGAUUUGGUUAGGCAGUAUAAAACUGUGACUUGAUGAUGGAUUUAUAAUUGAAAGCGCAUUACCGGCAUUUGCGAACGCCUGCAAGUGGAAAGCCCGUUGUAGGUUUUCGGGGCAAAAGUGCGGUCAAAAAUUCAAGUGUUUUCUAAUCACCGAAAAACAAAAGACCUGAAUCGGUUUCAGGUCUUUUUUAGCAUCAGAAAAUGCUUUA ...........(((((((...((((((..((....))..(((.(((((.(((((..(((..((......))..)))..(((((.....))))))))))..))))).))).....((((...((((((....((.....((((.((((((((.(((.....))))))))))).))))..))...)))))).)))).......)))))))))).)))..........((((((((((((.....))))))))))))..(((((......)))))... (-70.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 443 138 91 59 13 aact:AA01056|5end_conserved_hypothetical_protein_733867..734097_POSITIVE 419 99 58 83 40 aact:AA00952|5end_hemY_protein_homolog_657541..657771_POSITIVE 417 119 74 166 112 aact:AA01305|5end_conserved_hypothetical_protein_885598..885828_POSITIVE 409 117 72 166 113 aact:AA00993|5end_possible_D-tyrosyl-tRNA(Tyr)_deacylase_694488..694718_POSITIVE 407 128 83 160 114 aact:AA01832|5end_formate_dehydrogenase_formation_protein_E_1233609..1233839_POSITIVE 404 107 65 160 106 aact:AA02999|5end_conserved_hypothetical_protein_2094275..2094505_POSITIVE 385 100 57 228 178 aact:AA01145|5end_glycyl-tRNA_synthetase_alpha_subunit_783478..783708_POSITIVE 384 155 111 229 172 aact:AA01815|5end_probable_sodium-dependent_transmembrane_transport_protein_1222541..1222771_POSITIVE 381 155 120 50 6 aact:AA01644|5end_ferric_transport_system_permease_protein_1112050..1112280_POSITIVE 376 127 82 179 133 aact:AA01008|5end_mazG_beta-lactamase_regulatory_protein_homolog_703470..703700_POSITIVE 374 130 81 162 108 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 368 105 63 144 104 aact:AA02963|5end_opacity_associated_protein_B_2072302..2072532_POSITIVE 366 155 111 163 112 aact:AA02141|5end_ABC_transporter,_permease_protein_1464890..1465120_POSITIVE 365 99 52 142 99 aact:AA00419|5end_conserved_hypothetical_protein_(possible_phosphatidylethanolamine-binding_protein)_284337..284567_POSITIVE 360 104 63 52 12 aact:AA01448|5end_hypothetical_protein_970208..970438_POSITIVE 359 127 86 78 28 aact:AA01565|5end_conserved_hypothetical_protein_1050711..1050941_POSITIVE 356 158 113 159 111 aact:AA02049|5end_hypothetical_protein_1395526..1395756_POSITIVE 356 118 72 175 131 aact:AA01069|5end_translation_elongation_factor_(EF-Ts)_739775..740005_POSITIVE 356 118 72 69 25 aact:AA01068|5end_hypothetical_protein_739669..739899_POSITIVE 351 160 112 152 115 aact:AA01755|5end_cobalt_membrane_transport_protein_1185825..1186055_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 443 138 91 52 6 aact:AA01054|3end_conserved_hypothetical_protein_733940..734090_POSITIVE 417 119 74 68 14 aact:AA01304|3end_penicillin-binding_protein_1A_885580..885730_POSITIVE 407 128 83 80 34 aact:AA01830|3end_2,3-cyclic-nucleotide_2-phosphodiesterase_1233609..1233759_POSITIVE 407 100 57 67 23 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 400 99 58 141 96 aact:AA02998|3end_conserved_hypothetical_protein_2094350..2094500_POSITIVE 394 109 65 46 1 aact:AA00950|3end_conserved_hypothetical_protein_657574..657724_POSITIVE 387 107 72 148 113 aact:AA00992|3end_ribonuclease_BN_694568..694718_POSITIVE 376 127 82 75 29 aact:AA01006|3end_conserved_hypothetical_protein_(possible_cytosine_deaminas)_703446..703596_POSITIVE 374 130 81 101 47 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 368 105 63 47 7 aact:AA02962|3end_recombination_associated_protein_2072285..2072435_POSITIVE 368 155 113 136 101 aact:AA00218|3end_argininosuccinate_lyase_152211..152361_POSITIVE 363 130 83 137 78 aact:AA00574|3end_regulatory_protein_392381..392531_POSITIVE 360 104 63 90 50 aact:AA01446|3end_conserved_hypothetical_protein_970326..970476_POSITIVE 356 158 113 132 84 aact:AA02048|3end_galactitol_utilization_operon_repressor_1395579..1395729_POSITIVE 351 160 112 149 112 aact:AA01753|3end_conserved_hypothetical_protein_1185902..1186052_POSITIVE 351 117 71 139 96 aact:AA01400|3end_phosphopantetheine_adenylyltransferase_946241..946391_POSITIVE 350 170 132 147 110 aact:AA00583|3end_GlpG_protein_399210..399360_POSITIVE 347 96 57 69 32 aact:AA02035|3end_translation_initiation_factor_IF-3_1386905..1387055_POSITIVE 347 155 113 107 68 aact:AA00988|3end_conserved_hypothetical_protein_692264..692414_POSITIVE 345 95 57 76 41 aact:AA02112|3end_cell_division_protein_1442184..1442334_POSITIVE