Origin IGS: tagtgaaagcgtaaatccataaaatcgactaaaagtgcagtcattttttcaaacgaaataaccgcttttttcttgaatttttcattcatctcgataatatttatgaatatttattttatggatataacaag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cttgttatatccataaaataaatattcataaatattatcgagatgaatgaaaaattcaagaaaaaagcggttatttcgtttgaaaaaatgactgcacttttagtcgattttatggatttacgctttcacta Mask Tandem Repeat Region ================================================ tagtgaaagcgtaaatccataaaatcgactaaaagtgcagtcattttttcaaacgaaataaccgcttttttcttgaatttttcattcatctcgataatatttatgaatatttattttatggatataacaag Find is-nt database================================================ Query_seq: AA02794:AA02796|AA02794:AA02796:myo-inositol catabolism protein:possible transcriptional regulator, RpiR family:->->:1963010..1963140 131 tagtgaaagcgtaaatccataaaatcgactaaaagtgcagtcattttttcaaacgaaataaccgcttttttcttgaatttttcattcatctcgataatatttatgaatatttattttatggatataacaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02794:AA02796|AA02794:AA02796:myo-inositol catabolism protein:possible transcriptional regulator, RpiR family:->->:1963010..1963140 131 tagtgaaagcgtaaatccataaaatcgactaaaagtgcagtcattttttcaaacgaaataaccgcttttttcttgaatttttcattcatctcgataatatttatgaatatttattttatggatataacaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02794:AA02796|AA02794:AA02796:myo-inositol catabolism protein:possible transcriptional regulator, RpiR family:->->:1963010..1963140 131 tagtgaaagcgtaaatccataaaatcgactaaaagtgcagtcattttttcaaacgaaataaccgcttttttcttgaatttttcattcatctcgataatatttatgaatatttattttatggatataacaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagtgaaagcgtaaatccataaaatcgactaaaagtgcagtcattttttcaaacgaaataaccgcttttttcttgaatttttcattcatctcgataatatttatgaatatttattttatggatataacaag Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 19 End: 129 .................. M K S T K S A V I F S N E I T A F F L N F S F I S I I F M N I Y F M D I T .. Protein_Len: 36 Strand: - Start: 3 End: 110 .. H F R L D M F D V L L A T M K E F S I V A K K K F K E N M E I I N I F M ..................... tagtgaaagcgtaaatccataaaatcgactaaaagtgcagtcattttttcaaacgaaataaccgcttttttcttgaatttttcattcatctcgataatatttatgaatatttattttatggatataacaag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagtgaaagcgtaaatccataaaatcgactaaaagtgcagtcattttttcaaacgaaataaccgcttttttcttgaatttttcattcatctcgataatatttatgaatatttattttatggatataacaag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02794:AA02796|AA02794:AA02796:myo-inositol catabolism protein:possible transcriptional regulator, RpiR family:->->:1963010..1963140 131 tagtgaaagcgtaaatccataaaatcgactaaaagtgcagtcattttttcaaacgaaataaccgcttttttcttgaatttttcattcatctcgataatatttatgaatatttattttatggatataacaag Intra-Species Hit: Count: 1 Min: 1 Max: 131 Len: 131 Subject: aact_AA02794_AA02796|myo-inositol catabolism protein:possible transcriptional regulator, RpiR family|POSITIVE:POSITIVE|[1963010,1963140]|131 HSP 1 e-value: 1.0E-68 bit: 260.0 Len: 131 Query Start:1 Query End:131 Subject Strand: POSITIVE Subject Start: 1 Subject End: 131 tagtgaaagcgtaaatccataaaatcgactaaaagtgcagtcattttttcaaacgaaataaccgcttttttcttgaatttttcattcatctcgataatatttatgaatatttattttatggatataacaag tagtgaaagcgtaaatccataaaatcgactaaaagtgcagtcattttttcaaacgaaataaccgcttttttcttgaatttttcattcatctcgataatatttatgaatatttattttatggatataacaag Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUGAAAGCGUAAAUCCAUAAAAUCGACUAAAAGUGCAGUCAUUUUUUCAAACGAAAUAACCGCUUUUUUCUUGAAUUUUUCAUUCAUCUCGAUAAUAUUUAUGAAUAUUUAUUUUAUGGAUAUAACAAG ..((....))....((((((((((((((..(((((.((.((.(((((.......))))).)).)))))))..)))).......((((((.............)))))).....))))))))))........ (-19.92) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUUGUUAUAUCCAUAAAAUAAAUAUUCAUAAAUAUUAUCGAGAUGAAUGAAAAAUUCAAGAAAAAAGCGGUUAUUUCGUUUGAAAAAAUGACUGCACUUUUAGUCGAUUUUAUGGAUUUACGCUUUCACUA ...((...((((((((((..((((((....)))))).((((..(((((.....)))))........((((((((((.........))))))))))........))))))))))))))..)).......... (-27.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 294 76 35 194 142 aact:AA01724|5end_valyl-tRNA_synthetase_1164446..1164676_POSITIVE 274 50 9 97 58 aact:AA02577|5end_nuclease_1795267..1795497_POSITIVE 274 64 24 166 120 aact:AA01171|5end_LysA_activator_protein_802811..803041_POSITIVE 269 77 31 206 159 aact:AA02162|5end_lipoprotein_1478950..1479180_POSITIVE 268 55 26 226 198 aact:AA01609|5end_p-aminobenzoate_synthase_component_I_homolog_1087903..1088133_POSITIVE 255 70 29 193 151 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 249 48 2 110 57 aact:AA02097|5end_DedA-family_integral_membrane_protein_1428823..1429053_POSITIVE 238 73 31 227 174 aact:AA02347|5end_immunoglobulin_A1_protease_precursor_1603967..1604197_POSITIVE 238 73 31 79 26 aact:AA02346|5end_hypothetical_protein_1603819..1604049_POSITIVE 234 71 31 154 105 aact:AA01648|5end_alpha-amylase_protein;_1,4-alpha-D-glucan_glucanohydrolase_1115170..1115400_POSITIVE 234 71 31 53 4 aact:AA01646|5end_hypothetical_protein_1115069..1115299_POSITIVE 231 47 3 129 83 aact:AA00766|5end_heat_shock_protein_B25.3_(HSP-70_cofactor)_527384..527614_POSITIVE 231 55 22 102 66 aact:AA00618|5end_molybdopterin_biosynthesis_protein_422984..423214_POSITIVE 229 50 2 97 39 aact:AA00579|5end_probable_DNA_recombination_protein_395265..395495_POSITIVE 224 52 15 200 159 aact:AA01175|5end_6-phosphogluconate_dehydrogenase_804864..805094_POSITIVE 221 77 35 142 86 aact:AA02963|5end_opacity_associated_protein_B_2072302..2072532_POSITIVE 218 52 18 36 1 aact:AA01174|5end_hypothetical_protein_804700..804930_POSITIVE 217 75 36 48 8 aact:AA00323|5end_conserved_hypothetical_protein_(possible_SAM-dependent_methyltransferase)_219387..219617_POSITIVE 216 67 36 39 8 aact:AA02880|5end_50S_ribosomal_protein_L21_2020757..2020987_POSITIVE 214 95 58 221 184 aact:AA00108|5end_conserved_hypothetical_protein_77957..78187_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 269 77 31 105 58 aact:AA02160|3end_conserved_hypothetical_protein_1478929..1479079_POSITIVE 255 70 29 110 68 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 245 70 22 88 39 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 238 73 31 76 23 aact:AA02344|3end_GTP-binding_protein,_elongation_factor_typA/bipA_1603896..1604046_POSITIVE 234 94 60 125 95 aact:AA02539|3end_transcriptional_activator_1766929..1767079_POSITIVE 234 71 31 88 39 aact:AA01645|3end_ferric_transport_ABC-related_ATP-binding_protein_1115184..1115334_POSITIVE 230 76 36 143 103 aact:AA01069|3end_translation_elongation_factor_(EF-Ts)_740700..740850_POSITIVE 224 96 55 54 6 aact:AA00461|3end_heat_shock_protein;_protease_322979..323129_POSITIVE 212 44 4 131 94 aact:AA02171|3end_polysialic_acid_capsule_expression_protein_1486096..1486246_POSITIVE 211 65 23 144 106 aact:AA02878|3end_conserved_hypothetical_protein_2019598..2019748_POSITIVE 210 67 25 98 42 aact:AA01718|3end_DNA_polymerase_III,_chi_subunit_1163316..1163466_POSITIVE 210 59 31 76 49 aact:AA00046|3end_formate_dependent_nitrite_reductase,_cytochrome-c_type_protein_36756..36906_POSITIVE 207 90 44 87 41 aact:AA02046|3end_PTS_system_galactitol-specific_enzyme_IIC_1393687..1393837_POSITIVE 205 48 2 89 49 aact:AA00990|3end_conserved_hypothetical_protein_693265..693415_POSITIVE 205 51 18 77 46 aact:AA00342|3end_hypothetical_protein_229939..230089_POSITIVE 204 65 27 45 5 aact:AA01663|3end_GTP_cyclohydrolase_I_1128204..1128354_POSITIVE 204 72 31 81 35 aact:AA00896|3end_prolyl-tRNA_synthetase_(proline-tRNA_ligase)_623206..623356_POSITIVE 203 44 7 38 7 aact:AA00419|3end_conserved_hypothetical_protein_(possible_phosphatidylethanolamine-binding_protein)_284911..285061_POSITIVE 201 77 41 45 1 aact:AA02962|3end_recombination_associated_protein_2072285..2072435_POSITIVE 201 65 31 66 43 aact:AA01557|3end_recombinase_A_1045965..1046115_POSITIVE