Origin IGS: acatattctcctggttaatatatcgttgtaggtttacaacataaatgattacatgaaaacttgtgtgcttatcctccttgttcattttatgaacgcattcaaattaatcctgtcatttgaaaaatacaatacatataaaccctaaaatgaactttttattccacttttgtgatctgtatctgattttaaggaaaagccggtagattaattaagttaagggagtattatccaaggagtctattgagtcattttaggagaatgcg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cgcattctcctaaaatgactcaatagactccttggataatactcccttaacttaattaatctaccggcttttccttaaaatcagatacagatcacaaaagtggaataaaaagttcattttagggtttatatgtattgtatttttcaaatgacaggattaatttgaatgcgttcataaaatgaacaaggaggataagcacacaagttttcatgtaatcatttatgttgtaaacctacaacgatatattaaccaggagaatatgt Mask Tandem Repeat Region ================================================ acatattctcctggttaatatatcgttgtaggtttacaacataaatgattacatgaaaacttgtgtgcttatcctccttgttcattttatgaacgcattcaaattaatcctgtcatttgaaaaatacaatacatataaaccctaaaatgaactttttattccacttttgtgatctgtatctgattttaaggaaaagccggtagattaattaagttaagggagtattatccaaggagtctattgagtcattttaggagaatgcg Find is-nt database================================================ Query_seq: AA02793:AA02794|AA02793:AA02794:inositol-1-monophosphatase; extragenic suppressor protein:myo-inositol catabolism protein:->->:1961919..1962181 263 acatattctcctggttaatatatcgttgtaggtttacaacataaatgattacatgaaaacttgtgtgcttatcctccttgttcattttatgaacgcattcaaattaatcctgtcatttgaaaaatacaatacatataaaccctaaaatgaactttttattccacttttgtgatctgtatctgattttaaggaaaagccggtagattaattaagttaagggagtattatccaaggagtctattgagtcattttaggagaatgcg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02793:AA02794|AA02793:AA02794:inositol-1-monophosphatase; extragenic suppressor protein:myo-inositol catabolism protein:->->:1961919..1962181 263 acatattctcctggttaatatatcgttgtaggtttacaacataaatgattacatgaaaacttgtgtgcttatcctccttgttcattttatgaacgcattcaaattaatcctgtcatttgaaaaatacaatacatataaaccctaaaatgaactttttattccacttttgtgatctgtatctgattttaaggaaaagccggtagattaattaagttaagggagtattatccaaggagtctattgagtcattttaggagaatgcg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02793:AA02794|AA02793:AA02794:inositol-1-monophosphatase; extragenic suppressor protein:myo-inositol catabolism protein:->->:1961919..1962181 263 acatattctcctggttaatatatcgttgtaggtttacaacataaatgattacatgaaaacttgtgtgcttatcctccttgttcattttatgaacgcattcaaattaatcctgtcatttgaaaaatacaatacatataaaccctaaaatgaactttttattccacttttgtgatctgtatctgattttaaggaaaagccggtagattaattaagttaagggagtattatccaaggagtctattgagtcattttaggagaatgcg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acatattctcctggttaatatatcgttgtaggtttacaacataaatgattacatgaaaacttgtgtgcttatcctccttgttcattttatgaacgcattcaaattaatcctgtcatttgaaaaatacaatacatataaaccctaaaatgaactttttattccacttttgtgatctgtatctgattttaaggaaaagccggtagattaattaagttaagggagtattatccaaggagtctattgagtcattttaggagaatgcg Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 41 End: 142 ........................................ M N D Y M K T C V L I L L V H F M N A F K L I L S F E K Y N T Y K P ......................................................................................................................... Protein_Len: 30 Strand: - Start: 116 End: 205 ................................................................................................................... K F F V I C I F G L I F K K N W K Q S R Y R I K L F L R Y M .......................................................... acatattctcctggttaatatatcgttgtaggtttacaacataaatgattacatgaaaacttgtgtgcttatcctccttgttcattttatgaacgcattcaaattaatcctgtcatttgaaaaatacaatacatataaaccctaaaatgaactttttattccacttttgtgatctgtatctgattttaaggaaaagccggtagattaattaagttaagggagtattatccaaggagtctattgagtcattttaggagaatgcg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acatattctcctggttaatatatcgttgtaggtttacaacataaatgattacatgaaaacttgtgtgcttatcctccttgttcattttatgaacgcattcaaattaatcctgtcatttgaaaaatacaatacatataaaccctaaaatgaactttttattccacttttgtgatctgtatctgattttaaggaaaagccggtagattaattaagttaagggagtattatccaaggagtctattgagtcattttaggagaatgcg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02793:AA02794|AA02793:AA02794:inositol-1-monophosphatase; extragenic suppressor protein:myo-inositol catabolism protein:->->:1961919..1962181 263 acatattctcctggttaatatatcgttgtaggtttacaacataaatgattacatgaaaacttgtgtgcttatcctccttgttcattttatgaacgcattcaaattaatcctgtcatttgaaaaatacaatacatataaaccctaaaatgaactttttattccacttttgtgatctgtatctgattttaaggaaaagccggtagattaattaagttaagggagtattatccaaggagtctattgagtcattttaggagaatgcg Intra-Species Hit: Count: 1 Min: 1 Max: 263 Len: 263 Subject: aact_AA02793_AA02794|inositol-1-monophosphatase; extragenic suppressor protein:myo-inositol catabolism protein|POSITIVE:POSITIVE|[1961919,1962181]|263 HSP 1 e-value: 1.0E-147 bit: 521.0 Len: 263 Query Start:1 Query End:263 Subject Strand: POSITIVE Subject Start: 1 Subject End: 263 acatattctcctggttaatatatcgttgtaggtttacaacataaatgattacatgaaaacttgtgtgcttatcctccttgttcattttatgaacgcattcaaattaatcctgtcatttgaaaaatacaatacatataaaccctaaaatgaactttttattccacttttgtgatctgtatctgattttaaggaaaagccggtagattaattaagttaagggagtattatccaaggagtctattgagtcattttaggagaatgcg acatattctcctggttaatatatcgttgtaggtttacaacataaatgattacatgaaaacttgtgtgcttatcctccttgttcattttatgaacgcattcaaattaatcctgtcatttgaaaaatacaatacatataaaccctaaaatgaactttttattccacttttgtgatctgtatctgattttaaggaaaagccggtagattaattaagttaagggagtattatccaaggagtctattgagtcattttaggagaatgcg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACAUAUUCUCCUGGUUAAUAUAUCGUUGUAGGUUUACAACAUAAAUGAUUACAUGAAAACUUGUGUGCUUAUCCUCCUUGUUCAUUUUAUGAACGCAUUCAAAUUAAUCCUGUCAUUUGAAAAAUACAAUACAUAUAAACCCUAAAAUGAACUUUUUAUUCCACUUUUGUGAUCUGUAUCUGAUUUUAAGGAAAAGCCGGUAGAUUAAUUAAGUUAAGGGAGUAUUAUCCAAGGAGUCUAUUGAGUCAUUUUAGGAGAAUGCG ...((((((((.((((.((((...((((((....)))))).((((((((((((..(....)..))))............((((((...)))))).................)))))))).............)))).)))).((((((((.(((...(((((.(((...(((((((...(((.((((....))))..))))))))))...))).....(((......)))..))))).....))))))))))))))))))).. (-54.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGCAUUCUCCUAAAAUGACUCAAUAGACUCCUUGGAUAAUACUCCCUUAACUUAAUUAAUCUACCGGCUUUUCCUUAAAAUCAGAUACAGAUCACAAAAGUGGAAUAAAAAGUUCAUUUUAGGGUUUAUAUGUAUUGUAUUUUUCAAAUGACAGGAUUAAUUUGAAUGCGUUCAUAAAAUGAACAAGGAGGAUAAGCACACAAGUUUUCAUGUAAUCAUUUAUGUUGUAAACCUACAACGAUAUAUUAACCAGGAGAAUAUGU ...((((((((.((((((..((..(((((....(((......))).....................((((.(((((.......((((((((((.(.((((((((........)))))))).)))))....))))))......(((((((..........)))))))...((((((...))))))...))))).)))).....)))))...))...))))))..((((((....))))))............)))))))).... (-52.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 264 245 213 171 138 aact:AA00525|5end_hypothetical_protein_359254..359484_POSITIVE 263 236 196 95 59 aact:AA01263|5end_hypothetical_protein_860782..861012_POSITIVE 260 200 153 203 134 aact:AA02846|5end_crossover_junction_endodeoxyribonuclease_1998648..1998878_POSITIVE 248 206 161 210 164 aact:AA00663|5end_cystathionine_beta-lyase_455540..455770_POSITIVE 246 210 164 145 102 aact:AA02551|5end_iron_(chelated)_ABC_transporter,_permease_protein_1777641..1777871_POSITIVE 245 259 218 223 170 aact:AA02214|5end_pyruvate_kinase_II_1507248..1507478_POSITIVE 244 206 162 176 126 aact:AA02346|5end_hypothetical_protein_1603819..1604049_POSITIVE 242 201 162 193 155 aact:AA02316|5end_exodeoxyribonuclease_VII_large_subunit_1581682..1581912_POSITIVE 241 112 76 59 22 aact:AA02305|5end_guanosine-3',5'-bis(diphosphate)3'-_pyrophosphohydrolase_1574778..1575008_POSITIVE 241 83 60 173 144 aact:AA00432|5end_conserved_hypothetical_protein;_possible_methyltransferase_293897..294127_POSITIVE 241 96 51 60 19 aact:AA00250|5end_lipoate_biosynthesis_protein_A_175496..175726_POSITIVE 236 109 65 212 166 aact:AA01527|5end_hypothetical_protein_1025704..1025934_POSITIVE 235 208 161 129 77 aact:AA02072|5end_conserved_hypothetical_protein_1408601..1408831_POSITIVE 235 239 194 99 54 aact:AA01952|5end_ABC_transporter,_substrate_binding_protein_1318987..1319217_POSITIVE 234 240 198 72 36 aact:AA00856|5end_arginine_ABC_transporter,_periplasmic-binding_protein_590251..590481_POSITIVE 233 209 169 61 18 aact:AA02462|5end_thiol_peroxidase_(scavengase)_1702096..1702326_POSITIVE 231 240 197 59 19 aact:AA02889|5end_hypothetical_protein_2025045..2025275_POSITIVE 229 100 60 122 92 aact:AA02678|5end_formate_dehydrogenase,_gamma_subunit_1865021..1865251_POSITIVE 226 240 193 164 119 aact:AA02233|5end_asparaginyl-tRNA_synthetase_1519877..1520107_POSITIVE 226 117 77 161 114 aact:AA00341|5end_hypothetical_protein_229388..229618_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 260 200 153 136 67 aact:AA02845|3end_Holliday_junction_DNA_helicase_1998661..1998811_POSITIVE 248 206 161 117 71 aact:AA00662|3end_cystathionine_beta-lyase_455527..455677_POSITIVE 246 210 164 142 99 aact:AA02550|3end_iron_(chelated)_ABC_transporter,_ATP-binding_protein_1777718..1777868_POSITIVE 245 259 218 60 7 aact:AA02213|3end_hypothetical_protein_1507165..1507315_POSITIVE 241 112 76 55 18 aact:AA02303|3end_ATP-dependent_DNA_helicase_1574854..1575004_POSITIVE 239 98 57 50 7 aact:AA02568|3end_sugar_phosphotransferase_component_II_B_1788725..1788875_POSITIVE 235 239 194 76 31 aact:AA01950|3end_ABC_transporter,_membrane_spanning_protein_1319044..1319194_POSITIVE 234 240 198 64 28 aact:AA00855|3end_arginine_ABC_transporter,_permease_protein_590323..590473_POSITIVE 229 100 60 126 96 aact:AA02677|3end_formate_dehydrogenase,_beta_subunit_1865105..1865255_POSITIVE 226 117 77 118 71 aact:AA00340|3end_NADP(H)_oxidoreductase_(NAD(P)H_dehydrogenase_(quinone))_229425..229575_POSITIVE 225 200 160 148 110 aact:AA02982|3end_pyruvate_formate-lyase_activating_enzyme_2085127..2085277_POSITIVE 223 35 3 117 80 aact:AA01124.1|3end_biopolymer_transport_protein_D_772752..772902_POSITIVE 223 35 3 116 79 aact:AA01125|3end_hypothetical_protein_772751..772901_POSITIVE 220 88 44 107 62 aact:AA02169|3end_hypothetical_protein_1484664..1484814_POSITIVE 219 222 190 142 114 aact:AA00497|3end_hexose_phosphate_transport_system_regulatory_protein;_regulator_of_uhpT_expression_346479..346629_POSITIVE 216 207 162 111 51 aact:AA00423|3end_GTP-binding_protein_287224..287374_POSITIVE 215 206 161 59 22 aact:AA02830|3end_colicin_tolerance_protein_1990086..1990236_POSITIVE 214 203 161 140 104 aact:AA01969|3end_hypothetical_protein_1332165..1332315_POSITIVE 214 237 194 101 57 aact:AA00668|3end_cytolethal_distending_toxin_protein_B_459442..459592_POSITIVE 211 201 162 104 73 aact:AA00241|3end_formate_acetyltransferase_activating_enzyme_(anaerobic_ribonucleoside-triphosphate_reductase_activating_protein)_172090..172240_POSITIVE