Origin IGS: taatccgaaaagtgcggtcattttaaaaaacgttttttaaatcgaccgcacttttctcaacgacagaaacaaccacatcttttttctaccggatctgaagaagcttaaaaatcatcaaatctgctaaataaacaaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atttgtttatttagcagatttgatgatttttaagcttcttcagatccggtagaaaaaagatgtggttgtttctgtcgttgagaaaagtgcggtcgatttaaaaaacgttttttaaaatgaccgcacttttcggatta Mask Tandem Repeat Region ================================================ taatccgaaaagtgcggtcattttaaaaaacgttttttaaatcgaccgcacttttctcaacgacagaaacaaccacatcttttttctaccggatctgaagaagcttaaaaatcatcaaatctgctaaataaacaaat Find is-nt database================================================ Query_seq: AA02777:AA02778|AA02777:AA02778:tRNA-guanine transglycosylase:hypothetical protein:->->:1942588..1942724 137 taatccgaaaagtgcggtcattttaaaaaacgttttttaaatcgaccgcacttttctcaacgacagaaacaaccacatcttttttctaccggatctgaagaagcttaaaaatcatcaaatctgctaaataaacaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02777:AA02778|AA02777:AA02778:tRNA-guanine transglycosylase:hypothetical protein:->->:1942588..1942724 137 taatccgaaaagtgcggtcattttaaaaaacgttttttaaatcgaccgcacttttctcaacgacagaaacaaccacatcttttttctaccggatctgaagaagcttaaaaatcatcaaatctgctaaataaacaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02777:AA02778|AA02777:AA02778:tRNA-guanine transglycosylase:hypothetical protein:->->:1942588..1942724 137 taatccgaaaagtgcggtcattttaaaaaacgttttttaaatcgaccgcacttttctcaacgacagaaacaaccacatcttttttctaccggatctgaagaagcttaaaaatcatcaaatctgctaaataaacaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatccgaaaagtgcggtcattttaaaaaacgttttttaaatcgaccgcacttttctcaacgacagaaacaaccacatcttttttctaccggatctgaagaagcttaaaaatcatcaaatctgctaaataaacaaat Predict ORF larger than 30AA ================================================ taatccgaaaagtgcggtcattttaaaaaacgttttttaaatcgaccgcacttttctcaacgacagaaacaaccacatcttttttctaccggatctgaagaagcttaaaaatcatcaaatctgctaaataaacaaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatccgaaaagtgcggtcattttaaaaaacgttttttaaatcgaccgcacttttctcaacgacagaaacaaccacatcttttttctaccggatctgaagaagcttaaaaatcatcaaatctgctaaataaacaaat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02777:AA02778|AA02777:AA02778:tRNA-guanine transglycosylase:hypothetical protein:->->:1942588..1942724 137 taatccgaaaagtgcggtcattttaaaaaacgttttttaaatcgaccgcacttttctcaacgacagaaacaaccacatcttttttctaccggatctgaagaagcttaaaaatcatcaaatctgctaaataaacaaat Intra-Species Hit: Count: 1 Min: 1 Max: 137 Len: 137 Subject: aact_AA02777_AA02778|tRNA-guanine transglycosylase:hypothetical protein|POSITIVE:POSITIVE|[1942588,1942724]|137 HSP 1 e-value: 3.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 1 Subject End: 137 taatccgaaaagtgcggtcattttaaaaaacgttttttaaatcgaccgcacttttctcaacgacagaaacaaccacatcttttttctaccggatctgaagaagcttaaaaatcatcaaatctgctaaataaacaaat taatccgaaaagtgcggtcattttaaaaaacgttttttaaatcgaccgcacttttctcaacgacagaaacaaccacatcttttttctaccggatctgaagaagcttaaaaatcatcaaatctgctaaataaacaaat HSP 2 e-value: 8.0E-11 bit: 67.9 Len: 50 Query Start:7 Query End:56 Subject Strand: NEGATIVE Subject Start: 7 Subject End: 56 ......gaaaagtgcggtcattttaaaaaacgttttttaaatcgaccgcacttttc................................................................................. ......gaaaagtgcggtcgatttaaaaaacgttttttaaaatgaccgcacttttc................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCCGAAAAGUGCGGUCAUUUUAAAAAACGUUUUUUAAAUCGACCGCACUUUUCUCAACGACAGAAACAACCACAUCUUUUUUCUACCGGAUCUGAAGAAGCUUAAAAAUCAUCAAAUCUGCUAAAUAAACAAAU ......(((((((((((((..((((((((....))))))))..)))))))))))))........(((((.((.......)).)))))..(((((.(((.((..........)).))).))))).............. (-32.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUGUUUAUUUAGCAGAUUUGAUGAUUUUUAAGCUUCUUCAGAUCCGGUAGAAAAAAGAUGUGGUUGUUUCUGUCGUUGAGAAAAGUGCGGUCGAUUUAAAAAACGUUUUUUAAAAUGACCGCACUUUUCGGAUUA ................(((((((.((..........)).)))))))((..(((((...(((...))).)))))..))....((((((((((((((.((((((((....)))))))).))))))))))))))...... (-40.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 696 58 11 159 108 aact:AA01648|5end_alpha-amylase_protein;_1,4-alpha-D-glucan_glucanohydrolase_1115170..1115400_POSITIVE 696 58 11 58 7 aact:AA01646|5end_hypothetical_protein_1115069..1115299_POSITIVE 620 55 11 170 126 aact:AA00721|5end_probable_beta-N-acetylhexosaminidase_(lacto-N-biosidase);_dispersin_B_494739..494969_POSITIVE 620 55 11 58 14 aact:AA00719|5end_hypothetical_protein_494627..494857_POSITIVE 577 55 11 172 128 aact:AA01117|5end_signal_recognition_particle_protein_54_(GTP-binding_export_factor)_767919..768149_POSITIVE 574 57 11 163 115 aact:AA02700|5end_succinyl-CoA_synthase,_alpha_subunit_1883256..1883486_POSITIVE 522 54 11 159 116 aact:AA00766|5end_heat_shock_protein_B25.3_(HSP-70_cofactor)_527384..527614_POSITIVE 517 60 11 174 124 aact:AA00356|5end_DNA-directed_RNA_polymerase,_beta'_chain_238937..239167_POSITIVE 502 59 11 154 107 aact:AA02888|5end_ssrA-binding_protein,_small_protein_B_2024628..2024858_POSITIVE 480 54 11 151 108 aact:AA00386|5end_aspartate--ammonia_ligase_(asparagine_synthetase_A)_261150..261380_POSITIVE 479 55 11 164 120 aact:AA01967|5end_hypothetical_protein_1331511..1331741_POSITIVE 462 58 11 160 113 aact:AA02860|5end_conserved_hypothetical_protein_2006074..2006304_POSITIVE 432 57 12 157 116 aact:AA00300|5end_conserved_hypothetical_protein_203785..204015_POSITIVE 424 55 11 165 113 aact:AA02249|5end_thiamine_biosynthesis_protein_1528365..1528595_POSITIVE 420 55 11 195 154 aact:AA02902|5end_aerobic_respiration_control_protein_2031171..2031401_POSITIVE 387 54 11 165 122 aact:AA01981|5end_4-alpha-glucanotransferase_1337202..1337432_POSITIVE 381 60 11 165 101 aact:AA01059|5end_orotate_phosphoribosyltransferase_735052..735282_POSITIVE 380 55 11 76 35 aact:AA02002|5end_hypothetical_protein_1360196..1360426_POSITIVE 377 45 1 116 69 aact:AA02449|5end_hypothetical_protein_1687575..1687805_POSITIVE 377 67 21 140 89 aact:AA00792|5end_conserved_hypothetical_protein_543142..543372_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 696 58 11 93 42 aact:AA01645|3end_ferric_transport_ABC-related_ATP-binding_protein_1115184..1115334_POSITIVE 620 55 11 74 30 aact:AA00718|3end_ABC-related_ATP-binding_protein;_MRP_protein_494723..494873_POSITIVE 553 56 11 84 39 aact:AA02777|3end_tRNA-guanine_transglycosylase_1942527..1942677_POSITIVE 517 60 11 68 18 aact:AA00355|3end_DNA-directed_RNA_polymerase_beta_chain_(transcriptase_beta_chain)_238911..239061_POSITIVE 502 59 11 80 33 aact:AA02886|3end_OBG-related_GTP-binding_protein_2024634..2024784_POSITIVE 497 55 11 76 32 aact:AA00629|3end_inosine_5'_monophosphate_dehydrogenase_(IMP_dehydrogenase)_433469..433619_POSITIVE 480 54 11 89 46 aact:AA00384|3end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_261168..261318_POSITIVE 479 55 11 87 43 aact:AA01966|3end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_1331514..1331664_POSITIVE 477 54 13 42 1 aact:AA00764|3end_hemoglobin_binding_protein_A_527347..527497_POSITIVE 462 58 11 74 27 aact:AA02858|3end_conserved_hypothetical_protein_2006068..2006218_POSITIVE 462 42 6 151 115 aact:AA01646|3end_hypothetical_protein_1115262..1115412_POSITIVE 424 55 11 75 23 aact:AA02246|3end_peptidase_E_1528355..1528505_POSITIVE 420 55 11 82 41 aact:AA02899|3end_oligopeptide_ABC_transporter,_ATP-binding_protein_2031138..2031288_POSITIVE 388 57 11 62 19 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 387 54 11 140 97 aact:AA01978|3end_hypothetical_protein_1337257..1337407_POSITIVE 382 55 11 76 32 aact:AA02562|3end_adenine_specific_methylase_1785146..1785296_POSITIVE 381 60 11 80 16 aact:AA01057|3end_DnaJ-like_protein_djlA_735047..735197_POSITIVE 377 59 21 87 50 aact:AA02443|3end_cytochrome_C_related_protein_1686590..1686740_POSITIVE 377 59 21 132 95 aact:AA02444|3end_hypothetical_protein_1686635..1686785_POSITIVE 377 67 21 52 1 aact:AA00790|3end_recombination_DNA_repair_protein_543134..543284_POSITIVE