Origin IGS: taaataggncgacnaagccgattatacccctacaacaaatgcaattcattatcatctgaatatttattggtttacatcaaaaaaacatcgccaaaaaccaccgcactttagagaaaaatacgctccccgtcgccaaaagtgcggtcgtttttgcatttaaaaataaatccttgatcccgctcacaatatcaccacaagatgcttttcttattttgagcgtctttttacaatgaaagcgtttttaaaacattaaaaacaccattcacatttacttcagatatcacaagggtcttatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aataagacccttgtgatatctgaagtaaatgtgaatggtgtttttaatgttttaaaaacgctttcattgtaaaaagacgctcaaaataagaaaagcatcttgtggtgatattgtgagcgggatcaaggatttatttttaaatgcaaaaacgaccgcacttttggcgacggggagcgtatttttctctaaagtgcggtggtttttggcgatgtttttttgatgtaaaccaataaatattcagatgataatgaattgcatttgttgtaggggtataatcggcttngtcgncctattta Mask Tandem Repeat Region ================================================ taaataggncgacnaagccgattatacccctacaacaaatgcaattcattatcatctgaatatttattggtttacatcaaaaaaacatcgccaaaaaccaccgcactttagagaaaaatacgctccccgtcgccaaaagtgcggtcgtttttgcatttaaaaataaatccttgatcccgctcacaatatcaccacaagatgcttttcttattttgagcgtctttttacaatgaaagcgtttttaaaacattaaaaacaccattcacatttacttcagatatcacaagggtcttatt Find is-nt database================================================ Query_seq: AA02770:AA02772|AA02770:AA02772:hypothetical protein:hypothetical protein:->->:1936088..1936383 296 taaataggncgacnaagccgattatacccctacaacaaatgcaattcattatcatctgaatatttattggtttacatcaaaaaaacatcgccaaaaaccaccgcactttagagaaaaatacgctccccgtcgccaaaagtgcggtcgtttttgcatttaaaaataaatccttgatcccgctcacaatatcaccacaagatgcttttcttattttgagcgtctttttacaatgaaagcgtttttaaaacattaaaaacaccattcacatttacttcagatatcacaagggtcttatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02770:AA02772|AA02770:AA02772:hypothetical protein:hypothetical protein:->->:1936088..1936383 296 taaataggncgacnaagccgattatacccctacaacaaatgcaattcattatcatctgaatatttattggtttacatcaaaaaaacatcgccaaaaaccaccgcactttagagaaaaatacgctccccgtcgccaaaagtgcggtcgtttttgcatttaaaaataaatccttgatcccgctcacaatatcaccacaagatgcttttcttattttgagcgtctttttacaatgaaagcgtttttaaaacattaaaaacaccattcacatttacttcagatatcacaagggtcttatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02770:AA02772|AA02770:AA02772:hypothetical protein:hypothetical protein:->->:1936088..1936383 296 taaataggncgacnaagccgattatacccctacaacaaatgcaattcattatcatctgaatatttattggtttacatcaaaaaaacatcgccaaaaaccaccgcactttagagaaaaatacgctccccgtcgccaaaagtgcggtcgtttttgcatttaaaaataaatccttgatcccgctcacaatatcaccacaagatgcttttcttattttgagcgtctttttacaatgaaagcgtttttaaaacattaaaaacaccattcacatttacttcagatatcacaagggtcttatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaataggncgacnaagccgattatacccctacaacaaatgcaattcattatcatctgaatatttattggtttacatcaaaaaaacatcgccaaaaaccaccgcactttagagaaaaatacgctccccgtcgccaaaagtgcggtcgtttttgcatttaaaaataaatccttgatcccgctcacaatatcaccacaagatgcttttcttattttgagcgtctttttacaatgaaagcgtttttaaaacattaaaaacaccattcacatttacttcagatatcacaagggtcttatt Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 4 End: 108 ... M G R X S R L Y P Y N K C N S L S S E Y L L V Y I K K T S P K T T A L ............................................................................................................................................................................................ Protein_Len: 38 Strand: + Start: 44 End: 157 ........................................... M H Y H L N I Y W F T S K K H R Q K P P H F R E K Y A P R R Q K C G R F C I ........................................................................................................................................... Protein_Len: 57 Strand: + Start: 60 End: 230 ........................................................... M F I G L H Q K N I A K N H R T L E K N T L P V A K S A V V F A F K N K S L I P L T I S P Q D A F L I L S V F L Q .................................................................. Protein_Len: 44 Strand: - Start: 80 End: 211 ............................................................................... F F M A L F W R V K S F F V S G T A L L A T T K A N L F L D K I G S V I D G C S A K R M ..................................................................................... Protein_Len: 30 Strand: - Start: 67 End: 156 .................................................................. Q N V D F F C R W F G G C K L S F Y A G R R W F H P R K Q M ............................................................................................................................................ taaataggncgacnaagccgattatacccctacaacaaatgcaattcattatcatctgaatatttattggtttacatcaaaaaaacatcgccaaaaaccaccgcactttagagaaaaatacgctccccgtcgccaaaagtgcggtcgtttttgcatttaaaaataaatccttgatcccgctcacaatatcaccacaagatgcttttcttattttgagcgtctttttacaatgaaagcgtttttaaaacattaaaaacaccattcacatttacttcagatatcacaagggtcttatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaataggncgacnaagccgattatacccctacaacaaatgcaattcattatcatctgaatatttattggtttacatcaaaaaaacatcgccaaaaaccaccgcactttagagaaaaatacgctccccgtcgccaaaagtgcggtcgtttttgcatttaaaaataaatccttgatcccgctcacaatatcaccacaagatgcttttcttattttgagcgtctttttacaatgaaagcgtttttaaaacattaaaaacaccattcacatttacttcagatatcacaagggtcttatt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 74 HP_score: -7.6 Tail_Score: -3.9941 Start: 236 End: 259 Full_Region: aagtaaatgtgaatg gtgtttttaa tgtt ttaaaaacgc tttcattgtaaaaag ...........................................................................................................................................................................................................................................gtgtttttaatgttttaaaaacgc..................................... Find igs database================================================ Query_seq: AA02770:AA02772|AA02770:AA02772:hypothetical protein:hypothetical protein:->->:1936088..1936383 296 taaataggncgacnaagccgattatacccctacaacaaatgcaattcattatcatctgaatatttattggtttacatcaaaaaaacatcgccaaaaaccaccgcactttagagaaaaatacgctccccgtcgccaaaagtgcggtcgtttttgcatttaaaaataaatccttgatcccgctcacaatatcaccacaagatgcttttcttattttgagcgtctttttacaatgaaagcgtttttaaaacattaaaaacaccattcacatttacttcagatatcacaagggtcttatt Intra-Species Hit: Count: 1 Min: 1 Max: 296 Len: 296 Subject: aact_AA02770_AA02772|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1936088,1936383]|296 HSP 1 e-value: 1.0E-150 bit: 533.0 Len: 296 Query Start:1 Query End:296 Subject Strand: POSITIVE Subject Start: 1 Subject End: 296 taaataggncgacnaagccgattatacccctacaacaaatgcaattcattatcatctgaatatttattggtttacatcnnnnnnncatcgccaaaaaccaccgcactttagagaaaaatacgctccccgtcgccaaaagtgcggtcgtttttgcatttaaaaataaatccttgatcccgctcacaatatcaccacaagatgcttttcttattttgagcgtctttttacaatgaaagcgtttttaaaacattaaaaacaccattcacatttacttcagatatcacaagggtcttatt taaataggncgacnaagccgattatacccctacaacaaatgcaattcattatcatctgaatatttattggtttacatcaaaaaaacatcgccaaaaaccaccgcactttagagaaaaatacgctccccgtcgccaaaagtgcggtcgtttttgcatttaaaaataaatccttgatcccgctcacaatatcaccacaagatgcttttcttattttgagcgtctttttacaatgaaagcgtttttaaaacattaaaaacaccattcacatttacttcagatatcacaagggtcttatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUAGGNCGACNAAGCCGAUUAUACCCCUACAACAAAUGCAAUUCAUUAUCAUCUGAAUAUUUAUUGGUUUACAUCAAAAAAACAUCGCCAAAAACCACCGCACUUUAGAGAAAAAUACGCUCCCCGUCGCCAAAAGUGCGGUCGUUUUUGCAUUUAAAAAUAAAUCCUUGAUCCCGCUCACAAUAUCACCACAAGAUGCUUUUCUUAUUUUGAGCGUCUUUUUACAAUGAAAGCGUUUUUAAAACAUUAAAAACACCAUUCACAUUUACUUCAGAUAUCACAAGGGUCUUAUU ......((.(......)))........((((......((((.....))))...(((((((..(((((((......(((((.........((.((((((.((((((((((.(.(......(((.....)))..)).)))))))))).)))))))).((((....))))...)))))..((((((.((((........(((......))))))).))))))........)))))))...((((((((....))))))))...............)))))))......))))....... (-55.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAAGACCCUUGUGAUAUCUGAAGUAAAUGUGAAUGGUGUUUUUAAUGUUUUAAAAACGCUUUCAUUGUAAAAAGACGCUCAAAAUAAGAAAAGCAUCUUGUGGUGAUAUUGUGAGCGGGAUCAAGGAUUUAUUUUUAAAUGCAAAAACGACCGCACUUUUGGCGACGGGGAGCGUAUUUUUCUCUAAAGUGCGGUGGUUUUUGGCGAUGUUUUUUUGAUGUAAACCAAUAAAUAUUCAGAUGAUAAUGAAUUGCAUUUGUUGUAGGGGUAUAAUCGGCUUNGUCGNCCUAUUUA ......(((((((..(((((((((......(((((.(((((((((((....))))))))))))))))..........((((((.((((.......((((....)))).)))).))))))..((((((((..((((.......((((((((.((((((((((.......((((((.......)))))))))))))))).)))))).))))))..))))))))...............)))))))...((((.....)))).))..))))))).....((((...))))......... (-77.02) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 442 180 131 230 175 aact:AA01175|5end_6-phosphogluconate_dehydrogenase_804864..805094_POSITIVE 442 180 131 66 11 aact:AA01174|5end_hypothetical_protein_804700..804930_POSITIVE 422 109 80 38 9 aact:AA01660|5end_hypothetical_protein_1125035..1125265_POSITIVE 413 158 120 50 1 aact:AA02096|5end_hypothetical_protein_1428754..1428984_POSITIVE 400 158 126 119 83 aact:AA02097|5end_DedA-family_integral_membrane_protein_1428823..1429053_POSITIVE 397 152 121 172 139 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 396 147 101 197 158 aact:AA00008|5end_hypothetical_protein_(possible_transposase_fragment)_5032..5262_POSITIVE 390 136 94 184 149 aact:AA01981|5end_4-alpha-glucanotransferase_1337202..1337432_POSITIVE 390 136 94 37 2 aact:AA01978|5end_hypothetical_protein_1337055..1337285_POSITIVE 385 180 135 194 143 aact:AA03003|5end_GTP-binding_membrane_protein_2096544..2096774_POSITIVE 385 180 135 73 22 aact:AA03002|5end_hypothetical_protein_2096423..2096653_POSITIVE 383 154 121 179 144 aact:AA01991|5end_conserved_hypothetical_protein_(possible_nitroreductase)_1352372..1352602_POSITIVE 378 152 111 159 114 aact:AA02963|5end_opacity_associated_protein_B_2072302..2072532_POSITIVE 376 126 81 228 168 aact:AA01471|5end_type_I_restriction_enzyme_M_(modification_chain)_987321..987551_POSITIVE 371 154 119 64 24 aact:AA01538|5end_conserved_hypothetical_protein_1031527..1031757_POSITIVE 370 180 131 88 35 aact:AA02143|5end_hypothetical_protein_1467433..1467663_POSITIVE 366 133 91 64 20 aact:AA01566|5end_conserved_hypothetical_protein_1051811..1052041_POSITIVE 366 133 91 64 20 aact:AA01261|5end_conserved_hypothetical_protein_856443..856673_POSITIVE 364 122 81 125 81 aact:AA01008|5end_mazG_beta-lactamase_regulatory_protein_homolog_703470..703700_POSITIVE 362 150 101 164 117 aact:AA01519|5end_conserved_hypothetical_protein_1021794..1022024_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 397 152 121 67 34 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 396 147 101 140 101 aact:AA00007|3end_superoxide_dismutase_[Cu-Zn]_precursor_5055..5205_POSITIVE 389 136 98 34 4 aact:AA01976|3end_colicin_V_production_protein_1337132..1337282_POSITIVE 385 180 135 57 6 aact:AA03001|3end_probable_glycyl_radical_protein/pyruvate-formate_lyase_2096487..2096637_POSITIVE 378 152 111 62 17 aact:AA02962|3end_recombination_associated_protein_2072285..2072435_POSITIVE 371 154 119 64 24 aact:AA01537|3end_possible_glucokinase_regulatory_protein_1031607..1031757_POSITIVE 370 180 131 107 54 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 362 150 101 81 34 aact:AA01517|3end_peptidyl-prolyl_cis-trans_isomerase;_macrophage_infectivity_protein_1021791..1021941_POSITIVE 361 150 101 72 24 aact:AA00592|3end_conserved_hypothetical_protein_(membrane/transport_protein)_407286..407436_POSITIVE 359 110 65 145 104 aact:AA00507|3end_hypothetical_protein_353201..353351_POSITIVE 357 137 91 120 62 aact:AA00578|3end_DNA_topoisomerase_395201..395351_POSITIVE 348 154 125 80 39 aact:AA01280|3end_groES_chaperonin_868530..868680_POSITIVE 345 147 101 146 102 aact:AA01417|3end_probable_4-diphosphocytidyl-2C-methyl-D-erthyritol_synthase_(MEP_cytidyltransferase)_(MCT)_954028..954178_POSITIVE 345 180 133 112 56 aact:AA00508|3end_SAM-dependent_methyltransferase_353883..354033_POSITIVE 342 150 103 128 71 aact:AA00253|3end_hypothetical_protein_177636..177786_POSITIVE 340 109 68 64 23 aact:AA00675|3end_glutaredoxin_2_463248..463398_POSITIVE 335 109 86 68 45 aact:AA01179|3end_beta-ketoacyl-ACP_reductase;_short-chain_alcohol_dehydrogenase_808801..808951_POSITIVE 335 130 81 52 5 aact:AA00420|3end_tRNA/rRNA_methyltransferase_285506..285656_POSITIVE 334 159 124 145 107 aact:AA01160|3end_ADP_compounds_hydrolase_794190..794340_POSITIVE 333 109 66 84 36 aact:AA02940|3end_conserved_hypothetical_protein_2058652..2058802_POSITIVE