Origin IGS: tagtttatgaagttaantgataaccaaatttatattcttgttttatccgtgggtaaacggaatagattagancgcatacaanaanatgcagaattcagacaagctcggtaaaacgggctttttttaacccttgaatttacaatccaagtgcctctctgctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagcagagaggcacttggattgtaaattcaagggttaaaaaaagcccgttttaccgagcttgtctgaattctgcatnttnttgtatgcgntctaatctattccgtttacccacggataaaacaagaatataaatttggttatcanttaacttcataaacta Mask Tandem Repeat Region ================================================ tagtttatgaagttaantgataaccaaatttatattcttgttttatccgtgggtaaacggaatagattagancgcatacaanaanatgcagaattcagacaagctcggtaaaacgggctttttttaacccttgaatttacaatccaagtgcctctctgctta Find is-nt database================================================ Query_seq: AA02767:AA02768|AA02767:AA02768:conserved hypothetical protein (possible stability determinant):conserved hypothetical protein:->->:1935518..1935679 162 tagtttatgaagttaantgataaccaaatttatattcttgttttatccgtgggtaaacggaatagattagancgcatacaanaanatgcagaattcagacaagctcggtaaaacgggctttttttaacccttgaatttacaatccaagtgcctctctgctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02767:AA02768|AA02767:AA02768:conserved hypothetical protein (possible stability determinant):conserved hypothetical protein:->->:1935518..1935679 162 tagtttatgaagttaantgataaccaaatttatattcttgttttatccgtgggtaaacggaatagattagancgcatacaanaanatgcagaattcagacaagctcggtaaaacgggctttttttaacccttgaatttacaatccaagtgcctctctgctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02767:AA02768|AA02767:AA02768:conserved hypothetical protein (possible stability determinant):conserved hypothetical protein:->->:1935518..1935679 162 tagtttatgaagttaantgataaccaaatttatattcttgttttatccgtgggtaaacggaatagattagancgcatacaanaanatgcagaattcagacaagctcggtaaaacgggctttttttaacccttgaatttacaatccaagtgcctctctgctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagtttatgaagttaantgataaccaaatttatattcttgttttatccgtgggtaaacggaatagattagancgcatacaanaanatgcagaattcagacaagctcggtaaaacgggctttttttaacccttgaatttacaatccaagtgcctctctgctta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 7 End: 108 ...... M K L X D N Q I Y I L V L S V G K R N R L X R I Q X X A E F R Q A R ...................................................... Protein_Len: 37 Strand: - Start: 33 End: 143 ................................ I R T K D T P L R F L N S R M C X X A S N L C A R Y F P S K K V R S N V M ................... tagtttatgaagttaantgataaccaaatttatattcttgttttatccgtgggtaaacggaatagattagancgcatacaanaanatgcagaattcagacaagctcggtaaaacgggctttttttaacccttgaatttacaatccaagtgcctctctgctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagtttatgaagttaantgataaccaaatttatattcttgttttatccgtgggtaaacggaatagattagancgcatacaanaanatgcagaattcagacaagctcggtaaaacgggctttttttaacccttgaatttacaatccaagtgcctctctgctta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 79 HP_score: -3.9 Tail_Score: -5.43866 Start: 103 End: 118 Full_Region: GCAGAATTCAGACAA GCTCG GTAAAA CGGGC TTTTTTTAACCCTTG ......................................................................................................GCTCGGTAAAACGGGC............................................ Find igs database================================================ Query_seq: AA02767:AA02768|AA02767:AA02768:conserved hypothetical protein (possible stability determinant):conserved hypothetical protein:->->:1935518..1935679 162 tagtttatgaagttaantgataaccaaatttatattcttgttttatccgtgggtaaacggaatagattagancgcatacaanaanatgcagaattcagacaagctcggtaaaacgggctttttttaacccttgaatttacaatccaagtgcctctctgctta Intra-Species Hit: Count: 1 Min: 1 Max: 162 Len: 162 Subject: aact_AA02767_AA02768|conserved hypothetical protein (possible stability determinant):conserved hypothetical protein|POSITIVE:POSITIVE|[1935518,1935679]|162 HSP 1 e-value: 2.0E-67 bit: 256.0 Len: 162 Query Start:1 Query End:162 Subject Strand: POSITIVE Subject Start: 1 Subject End: 162 tagtttatgaagttaantgataaccaaatttatattcttgttttatccgtgggtaaacggaatagattagancgcatacaanaanatgcagaattcagacaagctcggtaaaacgggcnnnnnnnaacccttgaatttacaatccaagtgcctctctgctta tagtttatgaagttaantgataaccaaatttatattcttgttttatccgtgggtaaacggaatagattagancgcatacaanaanatgcagaattcagacaagctcggtaaaacgggctttttttaacccttgaatttacaatccaagtgcctctctgctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUUUAUGAAGUUAANUGAUAACCAAAUUUAUAUUCUUGUUUUAUCCGUGGGUAAACGGAAUAGAUUAGANCGCAUACAANAANAUGCAGAAUUCAGACAAGCUCGGUAAAACGGGCUUUUUUUAACCCUUGAAUUUACAAUCCAAGUGCCUCUCUGCUUA .....((((..((((.....))))...........(((.((((..(((((......)))))..)))).)))...)))).........(((((....(((.(((((((......))))))).))).....((((...........))))......)))))... (-28.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGCAGAGAGGCACUUGGAUUGUAAAUUCAAGGGUUAAAAAAAGCCCGUUUUACCGAGCUUGUCUGAAUUCUGCAUNUUNUUGUAUGCGNUCUAAUCUAUUCCGUUUACCCACGGAUAAAACAAGAAUAUAAAUUUGGUUAUCANUUAACUUCAUAAACUA ...((((((((((((((((..((......)).(((((......)))))......)))))..)))))...))))))......((((((....(((.......(((((......))))).......)))))))))....(((((.....))))).......... (-32.64) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 292 150 102 161 103 aact:AA01556|5end_ABC_transporter_ATP-binding_protein_1044218..1044448_POSITIVE 291 130 88 79 22 aact:AA01911|5end_hypothetical_protein_1287791..1288021_POSITIVE 274 117 87 164 127 aact:AA02667|5end_hypothetical_protein_1856609..1856839_POSITIVE 271 160 114 202 149 aact:AA02420|5end_ISRSO11_related_transposase_1662070..1662300_POSITIVE 261 150 105 229 178 aact:AA00172|5end_conserved_hypothetical_protein_(possible_regulator_protein_/_nitrogen_fixation_protein)_121778..122008_POSITIVE 257 120 97 166 140 aact:AA01305|5end_conserved_hypothetical_protein_885598..885828_POSITIVE 251 129 89 211 157 aact:AA02928|5end_conserved_hypothetical_protein_2048361..2048591_POSITIVE 251 129 89 83 29 aact:AA02927|5end_hypothetical_protein_2048233..2048463_POSITIVE 250 119 77 142 101 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 248 79 31 50 3 aact:AA02423|5end_undecaprenyl-phosphate_alpha-N-acetylglucosaminyltransferase_1669102..1669332_POSITIVE 247 149 102 177 138 aact:AA01269|5end_hypothetical_protein_863172..863402_POSITIVE 247 149 102 225 186 aact:AA01078|5end_transcriptional_regulator_(probable_MerR_family)_746873..747103_POSITIVE 239 132 94 175 136 aact:AA01260|5end_hypothetical_protein_855550..855780_POSITIVE 238 120 73 56 17 aact:AA02604|5end_thioredoxin_reductase_1815552..1815782_POSITIVE 235 75 35 68 41 aact:AA02236|5end_stringent_starvation_protein_A_1521919..1522149_POSITIVE 235 160 115 101 44 aact:AA01762|5end_hypothetical_protein_1190386..1190616_POSITIVE 235 149 130 220 201 aact:AA01263|5end_hypothetical_protein_860782..861012_POSITIVE 235 117 86 62 24 aact:AA00835|5end_UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapep_tide)_575212..575442_POSITIVE 234 127 86 223 177 aact:AA01609|5end_p-aminobenzoate_synthase_component_I_homolog_1087903..1088133_POSITIVE 229 126 86 206 163 aact:AA01745|5end_conserved_hypothetical_protein_(possible_peptide_chain_release_factor_2)_1180394..1180624_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 274 117 87 58 21 aact:AA02666|3end_DNA_helicase_II_1856583..1856733_POSITIVE 257 120 97 68 42 aact:AA01304|3end_penicillin-binding_protein_1A_885580..885730_POSITIVE 251 129 89 117 63 aact:AA02926|3end_hypothetical_protein_2048347..2048497_POSITIVE 250 119 77 59 18 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 250 120 86 67 29 aact:AA01149|3end_glycyl-tRNA_synthetase_beta_subunit_788185..788335_POSITIVE 247 149 102 142 103 aact:AA02342|3end_ISRSO11/IS150_related_transposase,_orfB_1600151..1600301_POSITIVE 247 149 102 142 103 aact:AA01268|3end_transposase_863217..863367_POSITIVE 247 149 102 142 103 aact:AA01077|3end_transposase_746870..747020_POSITIVE 247 149 102 142 103 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 235 75 35 53 26 aact:AA02235|3end_stringent_starvation_protein_B_1521984..1522134_POSITIVE 229 126 86 84 41 aact:AA01744|3end_peptide_chain_release_factor_RF-2_1180352..1180502_POSITIVE 226 123 86 132 91 aact:AA00982|3end_aldo-keto_reductase_687583..687733_POSITIVE 225 135 95 101 58 aact:AA00083|3end_hypothetical_protein_60851..61001_POSITIVE 221 74 35 121 85 aact:AA00372|3end_acetate_CoA-transferase_(alpha_subunit)_251078..251228_POSITIVE 220 159 113 91 51 aact:AA02230|3end_sugar_kinase_1517617..1517767_POSITIVE 220 150 105 126 76 aact:AA00031|3end_conserved_hypothetical_protein_25717..25867_POSITIVE 219 118 73 122 71 aact:AA00813|3end_hypothetical_protein_559021..559171_POSITIVE 218 123 97 71 44 aact:AA01897|3end_conserved_hypothetical_protein_1281284..1281434_POSITIVE 218 130 89 65 20 aact:AA01572|3end_peptide_ABC_transporter,_permease_protein_1059838..1059988_POSITIVE 217 130 88 145 94 aact:AA02645|3end_dTDP-4-rhamnose_reductase_1842419..1842569_POSITIVE