Origin IGS: gcctgcattatttgaatattttcnaaaatctggtagaanngatgcagaanttaggcagaanttgctgaagagcgtctggcaacccttnnngnatccgattgaggtaagccttgatgancttatannaactcactt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aagtgagttnntataagntcatcaaggcttacctcaatcggatncnnnaagggttgccagacgctcttcagcaanttctgcctaanttctgcatcnnttctaccagattttngaaaatattcaaataatgcaggc Mask Tandem Repeat Region ================================================ gcctgcattatttgaatattttcnaaaatctggtagaanngatgcagaanttaggcagaanttgctgaagagcgtctggcaacccttnnngnatccgattgaggtaagccttgatgancttatannaactcactt Find is-nt database================================================ Query_seq: AA02766:AA02767|AA02766:AA02767:hypothetical protein:conserved hypothetical protein (possible stability determinant):->->:1935206..1935340 135 gcctgcattatttgaatattttcnaaaatctggtagaanngatgcagaanttaggcagaanttgctgaagagcgtctggcaacccttnnngnatccgattgaggtaagccttgatgancttatannaactcactt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02766:AA02767|AA02766:AA02767:hypothetical protein:conserved hypothetical protein (possible stability determinant):->->:1935206..1935340 135 gcctgcattatttgaatattttcnaaaatctggtagaanngatgcagaanttaggcagaanttgctgaagagcgtctggcaacccttnnngnatccgattgaggtaagccttgatgancttatannaactcactt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02766:AA02767|AA02766:AA02767:hypothetical protein:conserved hypothetical protein (possible stability determinant):->->:1935206..1935340 135 gcctgcattatttgaatattttcnaaaatctggtagaanngatgcagaanttaggcagaanttgctgaagagcgtctggcaacccttnnngnatccgattgaggtaagccttgatgancttatannaactcactt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gcctgcattatttgaatattttcnaaaatctggtagaanngatgcagaanttaggcagaanttgctgaagagcgtctggcaacccttnnngnatccgattgaggtaagccttgatgancttatannaactcactt Predict ORF larger than 30AA ================================================ Protein_Len: 41 Strand: - Start: 11 End: 133 .......... K F I K X F R T S X I C F X P L X Q Q L A D P L G X X X R N L Y A K I X K Y X S M .. Protein_Len: 41 Strand: - Start: 1 End: 123 G A N N S Y K X F D P L X S A S X L C F X S F L T Q C G K X X G I S T L R S S X M ............ gcctgcattatttgaatattttcnaaaatctggtagaanngatgcagaanttaggcagaanttgctgaagagcgtctggcaacccttnnngnatccgattgaggtaagccttgatgancttatannaactcactt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gcctgcattatttgaatattttcnaaaatctggtagaanngatgcagaanttaggcagaanttgctgaagagcgtctggcaacccttnnngnatccgattgaggtaagccttgatgancttatannaactcactt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02766:AA02767|AA02766:AA02767:hypothetical protein:conserved hypothetical protein (possible stability determinant):->->:1935206..1935340 135 gcctgcattatttgaatattttcnaaaatctggtagaanngatgcagaanttaggcagaanttgctgaagagcgtctggcaacccttnnngnatccgattgaggtaagccttgatgancttatannaactcactt Intra-Species Hit: Count: 1 Min: 1 Max: 135 Len: 135 Subject: aact_AA02766_AA02767|hypothetical protein:conserved hypothetical protein (possible stability determinant)|POSITIVE:POSITIVE|[1935206,1935340]|135 HSP 1 e-value: 1.0E-49 bit: 196.0 Len: 135 Query Start:1 Query End:135 Subject Strand: POSITIVE Subject Start: 1 Subject End: 135 gcctgcattatttgaatattttcnaaaatctggtagaanngatgcagaanttaggcagaanttgctgaagagcgtctggcaacccttnnngnatccgattgaggtaagccttgatgancttatannaactcactt gcctgcattatttgaatattttcnaaaatctggtagaanngatgcagaanttaggcagaanttgctgaagagcgtctggcaacccttnnngnatccgattgaggtaagccttgatgancttatannaactcactt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCCUGCAUUAUUUGAAUAUUUUCNAAAAUCUGGUAGAANNGAUGCAGAANUUAGGCAGAANUUGCUGAAGAGCGUCUGGCAACCCUUNNNGNAUCCGAUUGAGGUAAGCCUUGAUGANCUUAUANNAACUCACUU ..(((((((.((((...((((....))))....))))...)))))))......(.((((...((((....)))))))).).................((..(((....)))..)).................... (-22.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGUGAGUUNNUAUAAGNUCAUCAAGGCUUACCUCAAUCGGAUNCNNNAAGGGUUGCCAGACGCUCUUCAGCAANUUCUGCCUAANUUCUGCAUCNNUUCUACCAGAUUUUNGAAAAUAUUCAAAUAAUGCAGGC ..(((((((...((......))...)))))))......((((......((((((........))))))........)))).......(((((((....((.....)).....(((....))).....))))))). (-16.34) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 333 117 71 186 138 aact:AA01957|5end_conserved_hypothetical_protein_1323211..1323441_POSITIVE 318 109 62 211 163 aact:AA02337|5end_DNA_mismatch_repair_protein_1597350..1597580_POSITIVE 292 67 30 86 44 aact:AA01780|5end_ABC-related_molybdenum_transport_system_permease_protein_1201084..1201314_POSITIVE 275 85 43 88 45 aact:AA01873|5end_mannose-6-phosphate_isomerase_1260658..1260888_POSITIVE 275 109 64 50 3 aact:AA01098|5end_bis(5'-nucleosyl)-tetraphosphatase_757699..757929_POSITIVE 268 117 72 68 19 aact:AA02057|5end_phosphoglycerate_mutase_1401253..1401483_POSITIVE 256 85 43 65 34 aact:AA00846|5end_cell_division_protein_L_584643..584873_POSITIVE 255 97 54 61 23 aact:AA00275|5end_conserved_hypothetical_protein_192636..192866_POSITIVE 254 110 63 210 169 aact:AA00083|5end_hypothetical_protein_60664..60894_POSITIVE 253 109 69 156 120 aact:AA02898|5end_oligopeptide_transport_ATP-binding_protein_2029083..2029313_POSITIVE 253 77 31 155 112 aact:AA02262|5end_50S_ribosomal_protein_L13_1538422..1538652_POSITIVE 253 110 62 99 47 aact:AA01586|5end_DNA_mismatch_repair_protein_1066966..1067196_POSITIVE 252 99 58 46 2 aact:AA02973|5end_tyrosine-specific_transport_protein_2077707..2077937_POSITIVE 245 117 71 107 55 aact:AA02772|5end_hypothetical_protein_1936244..1936474_POSITIVE 244 112 71 149 105 aact:AA00170|5end_hypothetical_protein_121238..121468_POSITIVE 243 80 32 87 34 aact:AA01357|5end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919133..919363_POSITIVE 243 109 64 176 135 aact:AA01131|5end_conserved_hypothetical_protein_775275..775505_POSITIVE 242 99 54 65 9 aact:AA00042|5end_hypothetical_protein_33810..34040_POSITIVE 239 80 32 83 28 aact:AA00519|5end_O-acetyltransferase_357139..357369_POSITIVE 237 80 43 137 99 aact:AA00056|5end_cytochrome_c-type_biogenesis_protein_43925..44155_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 292 67 30 96 54 aact:AA01779|3end_molybdenum_ABC_transporter,_ATP-binding_protein_1201174..1201324_POSITIVE 269 75 31 114 77 aact:AA00988|3end_conserved_hypothetical_protein_692264..692414_POSITIVE 256 85 43 72 41 aact:AA00845|3end_hypothetical_protein_584730..584880_POSITIVE 255 110 63 83 26 aact:AA01651|3end_hypothetical_protein_1120004..1120154_POSITIVE 255 97 54 41 3 aact:AA00274|3end_50S_ribosomal_protein_L32_192696..192846_POSITIVE 253 109 69 143 107 aact:AA02897|3end_oligopeptide_ABC_transporter;_permease_protein_2029150..2029300_POSITIVE 253 77 31 135 92 aact:AA02261|3end_30S_ribosomal_protein_S9_1538482..1538632_POSITIVE 253 110 62 126 74 aact:AA01585|3end_tRNA_delta(2)-isopentenylpyrophosphate_1067073..1067223_POSITIVE 244 98 55 124 87 aact:AA02014|3end_probable_hemolysin_1370977..1371127_POSITIVE 239 80 32 77 22 aact:AA00518|3end_conserved_hypothetical_protein_357213..357363_POSITIVE 237 80 43 134 96 aact:AA00055|3end_cytochrome_c-type_biogenesis_protein_44002..44152_POSITIVE 235 109 63 74 13 aact:AA01018|3end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_712300..712450_POSITIVE 234 116 71 131 93 aact:AA02150|3end_conserved_hypothetical_protein_1470985..1471135_POSITIVE 230 87 43 76 29 aact:AA02648|3end_lytic_transglycosylase_1845637..1845787_POSITIVE 227 100 52 125 80 aact:AA02048|3end_galactitol_utilization_operon_repressor_1395579..1395729_POSITIVE 227 110 62 144 101 aact:AA00943|3end_conserved_hypothetical_protein_654542..654692_POSITIVE 223 85 41 70 23 aact:AA01874|3end_conserved_hypothetical_protein_1262451..1262601_POSITIVE 223 81 54 66 36 aact:AA01269|3end_hypothetical_protein_863413..863563_POSITIVE 222 85 41 102 40 aact:AA01744|3end_peptide_chain_release_factor_RF-2_1180352..1180502_POSITIVE 221 81 43 150 116 aact:AA02278|3end_conserved_hypothetical_protein_1551033..1551183_POSITIVE