Origin IGS: gaaaattaacttttgtggttaaatcagggttgtcagttttaccataatttacacagctcatatattcagtacatgagctattaatttaactaatttagcatcggagtactct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agagtactccgatgctaaattagttaaattaatagctcatgtactgaatatatgagctgtgtaaattatggtaaaactgacaaccctgatttaaccacaaaagttaattttc Mask Tandem Repeat Region ================================================ gaaaattaacttttgtggttaaatcagggttgtcagttttaccataatttacacagctcatatattcagtacatgagctattaatttaactaatttagcatcggagtactct Find is-nt database================================================ Query_seq: AA02750:AA02751|AA02750:AA02751:hypothetical protein:L-lactate permease:->->:1927230..1927341 112 gaaaattaacttttgtggttaaatcagggttgtcagttttaccataatttacacagctcatatattcagtacatgagctattaatttaactaatttagcatcggagtactct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02750:AA02751|AA02750:AA02751:hypothetical protein:L-lactate permease:->->:1927230..1927341 112 gaaaattaacttttgtggttaaatcagggttgtcagttttaccataatttacacagctcatatattcagtacatgagctattaatttaactaatttagcatcggagtactct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02750:AA02751|AA02750:AA02751:hypothetical protein:L-lactate permease:->->:1927230..1927341 112 gaaaattaacttttgtggttaaatcagggttgtcagttttaccataatttacacagctcatatattcagtacatgagctattaatttaactaatttagcatcggagtactct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gaaaattaacttttgtggttaaatcagggttgtcagttttaccataatttacacagctcatatattcagtacatgagctattaatttaactaatttagcatcggagtactct Predict ORF larger than 30AA ================================================ gaaaattaacttttgtggttaaatcagggttgtcagttttaccataatttacacagctcatatattcagtacatgagctattaatttaactaatttagcatcggagtactct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gaaaattaacttttgtggttaaatcagggttgtcagttttaccataatttacacagctcatatattcagtacatgagctattaatttaactaatttagcatcggagtactct Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02750:AA02751|AA02750:AA02751:hypothetical protein:L-lactate permease:->->:1927230..1927341 112 gaaaattaacttttgtggttaaatcagggttgtcagttttaccataatttacacagctcatatattcagtacatgagctattaatttaactaatttagcatcggagtactct Intra-Species Hit: Count: 1 Min: 1 Max: 112 Len: 112 Subject: aact_AA02750_AA02751|hypothetical protein:L-lactate permease|POSITIVE:POSITIVE|[1927230,1927341]|112 HSP 1 e-value: 2.0E-57 bit: 222.0 Len: 112 Query Start:1 Query End:112 Subject Strand: POSITIVE Subject Start: 1 Subject End: 112 gaaaattaacttttgtggttaaatcagggttgtcagttttaccataatttacacagctcatatattcagtacatgagctattaatttaactaatttagcatcggagtactct gaaaattaacttttgtggttaaatcagggttgtcagttttaccataatttacacagctcatatattcagtacatgagctattaatttaactaatttagcatcggagtactct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAAAAUUAACUUUUGUGGUUAAAUCAGGGUUGUCAGUUUUACCAUAAUUUACACAGCUCAUAUAUUCAGUACAUGAGCUAUUAAUUUAACUAAUUUAGCAUCGGAGUACUCU (((..((((((.....))))))....(((((((.((((.......))))...))))))).....))).((((.(((((((..((((.....)))))))).)))..))))... (-19.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAGUACUCCGAUGCUAAAUUAGUUAAAUUAAUAGCUCAUGUACUGAAUAUAUGAGCUGUGUAAAUUAUGGUAAAACUGACAACCCUGAUUUAACCACAAAAGUUAAUUUUC ..((((......)))).........((((((((.(((((((((.....))))))))).((((((((((.(((..........))).)))))))..)))....)))))))).. (-22.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 279 81 46 44 7 aact:AA01636|5end_conserved_hypothetical_protein_1105947..1106177_POSITIVE 279 45 5 75 39 aact:AA00741|5end_possible_2-oxoglutarate/malate_transporter_506659..506889_POSITIVE 236 58 12 117 67 aact:AA01160|5end_ADP_compounds_hydrolase_793565..793795_POSITIVE 229 73 31 138 95 aact:AA01769|5end_hypothetical_protein_1192948..1193178_POSITIVE 221 56 11 183 131 aact:AA00458|5end_nickel-binding_periplasmic_protein_319374..319604_POSITIVE 218 44 12 36 2 aact:AA01372|5end_ribonuclease_r,_virulence_associated_protein_927881..928111_POSITIVE 215 69 25 192 144 aact:AA00606|5end_membrane-associated_zinc_metalloprotease_411808..412038_POSITIVE 214 69 26 200 152 aact:AA00750|5end_periplasmic_D-galactose-binding_ABC_transport_protein_514904..515134_POSITIVE 210 70 21 145 94 aact:AA00899|5end_possible_quaternary_ammonium_compound_resistance_protein_623214..623444_POSITIVE 210 60 14 155 106 aact:AA00229|5end_DNA_polymerase_III,_alpha_subunit_156863..157093_POSITIVE 209 49 12 69 28 aact:AA02416|5end_conserved_hypothetical_protein_1658920..1659150_POSITIVE 209 56 13 200 160 aact:AA01032|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_B_720930..721160_POSITIVE 208 104 67 113 72 aact:AA00170|5end_hypothetical_protein_121238..121468_POSITIVE 207 60 11 171 124 aact:AA01270|5end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863317..863547_POSITIVE 205 60 18 179 138 aact:AA00069|5end_conserved_hypothetical_protein_52465..52695_POSITIVE 203 96 55 145 103 aact:AA00226|5end_hypothetical_protein_156276..156506_POSITIVE 200 54 27 215 195 aact:AA02112|5end_cell_division_protein_1437617..1437847_POSITIVE 200 109 66 156 109 aact:AA00018|5end_hypothetical_protein_10550..10780_POSITIVE 199 98 55 100 59 aact:AA00145|5end_probable_degenerate_transposase_fragment_105046..105276_POSITIVE 199 78 31 144 99 aact:AA00077|5end_conserved_hypothetical_protein_(possible_periplasmic_binding_transport_protein)_56111..56341_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 279 45 5 85 49 aact:AA00740|3end_citrate_lyase_related_biosynthesis_protein_(2-(5'-triphosphoribosyl)-3'-dephosphocoenzyme-A_synthase)_506749..506899_POSITIVE 236 58 12 123 73 aact:AA01159|3end_phosphatase_793651..793801_POSITIVE 229 73 31 71 28 aact:AA01767|3end_conserved_hypothetical_protein_1192961..1193111_POSITIVE 221 56 11 114 62 aact:AA00455|3end_lipoprotein_precursor_319385..319535_POSITIVE 211 58 16 66 38 aact:AA02918|3end_hypothetical_protein_2043501..2043651_POSITIVE 210 70 21 57 6 aact:AA00896|3end_prolyl-tRNA_synthetase_(proline-tRNA_ligase)_623206..623356_POSITIVE 203 58 15 142 79 aact:AA02145|3end_iron(III)_ABC_transporter,_ATP-binding_protein_1469176..1469326_POSITIVE 191 60 25 39 8 aact:AA02898|3end_oligopeptide_transport_ATP-binding_protein_2030143..2030293_POSITIVE 191 57 11 145 93 aact:AA00515|3end_hypothetical_protein_355959..356109_POSITIVE 190 70 25 66 25 aact:AA01959|3end_tetraacyldisaccharide_4'-kinase_1324463..1324613_POSITIVE 189 44 9 134 110 aact:AA01069|3end_translation_elongation_factor_(EF-Ts)_740700..740850_POSITIVE 188 59 11 127 74 aact:AA01585|3end_tRNA_delta(2)-isopentenylpyrophosphate_1067073..1067223_POSITIVE 187 73 37 119 80 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 186 108 73 123 91 aact:AA02494|3end_conserved_hypothetical_protein_1731540..1731690_POSITIVE 184 70 26 137 100 aact:AA02031|3end_type_III_site-specific_deoxyribonuclease_1385155..1385305_POSITIVE 184 37 15 103 81 aact:AA00391|3end_arsenate_reductase_265248..265398_POSITIVE 183 60 14 87 41 aact:AA01083|3end_preprotein_translocase_SecA_subunit_750970..751120_POSITIVE 183 59 25 129 99 aact:AA01046|3end_conserved_hypothetical_protein_729280..729430_POSITIVE 183 58 11 143 87 aact:AA00649|3end_peptide_chain_release_factor_1_(RF-1)_444355..444505_POSITIVE 181 57 16 124 76 aact:AA02135|3end_hypothetical_protein_1459067..1459217_POSITIVE