Origin IGS: taaccaatttattttacaacctaactcctgaggtcaatttacgatgcctgatattaaactcttcgccggtaacgccacaccggagctcgcgaaacgcatttccgaaaacctgttcaccaaattaggtgatgccacc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ggtggcatcacctaatttggtgaacaggttttcggaaatgcgtttcgcgagctccggtgtggcgttaccggcgaagagtttaatatcaggcatcgtaaattgacctcaggagttaggttgtaaaataaattggtta Mask Tandem Repeat Region ================================================ taaccaatttattttacaacctaactcctgaggtcaatttacgatgcctgatattaaactcttcgccggtaacgccacaccggagctcgcgaaacgcatttccgaaaacctgttcaccaaattaggtgatgccacc Find is-nt database================================================ Query_seq: AA02745:AA02747|AA02745:AA02747:isopentenyl monophosphate kinase:ribose-phosphate pyrophosphokinase; phosphoribosylpyrophosphate synthase:->->:1924328..1924463 136 taaccaatttattttacaacctaactcctgaggtcaatttacgatgcctgatattaaactcttcgccggtaacgccacaccggagctcgcgaaacgcatttccgaaaacctgttcaccaaattaggtgatgccacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02745:AA02747|AA02745:AA02747:isopentenyl monophosphate kinase:ribose-phosphate pyrophosphokinase; phosphoribosylpyrophosphate synthase:->->:1924328..1924463 136 taaccaatttattttacaacctaactcctgaggtcaatttacgatgcctgatattaaactcttcgccggtaacgccacaccggagctcgcgaaacgcatttccgaaaacctgttcaccaaattaggtgatgccacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02745:AA02747|AA02745:AA02747:isopentenyl monophosphate kinase:ribose-phosphate pyrophosphokinase; phosphoribosylpyrophosphate synthase:->->:1924328..1924463 136 taaccaatttattttacaacctaactcctgaggtcaatttacgatgcctgatattaaactcttcgccggtaacgccacaccggagctcgcgaaacgcatttccgaaaacctgttcaccaaattaggtgatgccacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaccaatttattttacaacctaactcctgaggtcaatttacgatgcctgatattaaactcttcgccggtaacgccacaccggagctcgcgaaacgcatttccgaaaacctgttcaccaaattaggtgatgccacc Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 44 End: 136 ........................................... M P D I K L F A G N A T P E L A K R I S E N L F T K L G D A T Protein_Len: 33 Strand: - Start: 37 End: 135 .................................... N V I G S I L S K A P L A V G S S A F R M E S F R N V L N P S A M . taaccaatttattttacaacctaactcctgaggtcaatttacgatgcctgatattaaactcttcgccggtaacgccacaccggagctcgcgaaacgcatttccgaaaacctgttcaccaaattaggtgatgccacc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaccaatttattttacaacctaactcctgaggtcaatttacgatgcctgatattaaactcttcgccggtaacgccacaccggagctcgcgaaacgcatttccgaaaacctgttcaccaaattaggtgatgccacc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02745:AA02747|AA02745:AA02747:isopentenyl monophosphate kinase:ribose-phosphate pyrophosphokinase; phosphoribosylpyrophosphate synthase:->->:1924328..1924463 136 taaccaatttattttacaacctaactcctgaggtcaatttacgatgcctgatattaaactcttcgccggtaacgccacaccggagctcgcgaaacgcatttccgaaaacctgttcaccaaattaggtgatgccacc Intra-Species Hit: Count: 1 Min: 1 Max: 136 Len: 136 Subject: aact_AA02745_AA02747|isopentenyl monophosphate kinase:ribose-phosphate pyrophosphokinase; phosphoribosylpyrophosphate synthase|POSITIVE:POSITIVE|[1924328,1924463]|136 HSP 1 e-value: 1.0E-71 bit: 270.0 Len: 136 Query Start:1 Query End:136 Subject Strand: POSITIVE Subject Start: 1 Subject End: 136 taaccaatttattttacaacctaactcctgaggtcaatttacgatgcctgatattaaactcttcgccggtaacgccacaccggagctcgcgaaacgcatttccgaaaacctgttcaccaaattaggtgatgccacc taaccaatttattttacaacctaactcctgaggtcaatttacgatgcctgatattaaactcttcgccggtaacgccacaccggagctcgcgaaacgcatttccgaaaacctgttcaccaaattaggtgatgccacc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACCAAUUUAUUUUACAACCUAACUCCUGAGGUCAAUUUACGAUGCCUGAUAUUAAACUCUUCGCCGGUAACGCCACACCGGAGCUCGCGAAACGCAUUUCCGAAAACCUGUUCACCAAAUUAGGUGAUGCCACC ..............................((((........(((((..((........)).((((.(((..((......))..))).))))...))))).......))))..(((((......)))))....... (-22.06) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUGGCAUCACCUAAUUUGGUGAACAGGUUUUCGGAAAUGCGUUUCGCGAGCUCCGGUGUGGCGUUACCGGCGAAGAGUUUAAUAUCAGGCAUCGUAAAUUGACCUCAGGAGUUAGGUUGUAAAAUAAAUUGGUUA ((((.(.(((((......)))))..((.((((((....((((...))))....((((((......)))))))))))).))........).)))).......(((((........)))))................. (-32.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 447 110 63 150 95 aact:AA00989|5end_hypothetical_protein_692319..692549_POSITIVE 415 106 65 60 17 aact:AA00212|5end_ribokinase_146056..146286_POSITIVE 408 89 42 193 146 aact:AA01589|5end_conserved_hypothetical_protein_1070300..1070530_POSITIVE 404 105 64 52 13 aact:AA00816|5end_lacZ_expression_regulator_560900..561130_POSITIVE 396 110 62 49 15 aact:AA02367|5end_conserved_hypothetical_protein_1623174..1623404_POSITIVE 395 90 42 227 177 aact:AA01247|5end_hypothetical_protein_849664..849894_POSITIVE 386 105 65 124 81 aact:AA01527|5end_hypothetical_protein_1025704..1025934_POSITIVE 383 110 61 209 161 aact:AA02575|5end_transmembrane_protein_1793749..1793979_POSITIVE 376 97 57 69 21 aact:AA01772|5end_shikimate_5-dehydrogenase_protein_1194050..1194280_POSITIVE 374 110 61 62 7 aact:AA00323|5end_conserved_hypothetical_protein_(possible_SAM-dependent_methyltransferase)_219387..219617_POSITIVE 369 90 41 66 27 aact:AA02954|5end_penicillin_tolerance_protein;_hydroxymethylbutenyl_pyrophosphate_reductase_2065610..2065840_POSITIVE 368 96 58 67 15 aact:AA02466|5end_N-acetylneuraminate_lyase_1704963..1705193_POSITIVE 365 104 65 71 15 aact:AA01270|5end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863317..863547_POSITIVE 365 102 64 55 8 aact:AA00475|5end_conserved_hypothetical_protein_(possible_N6-adenine-specific_methylase)_331621..331851_POSITIVE 362 110 64 175 106 aact:AA00576|5end_conserved_hypothetical_protein_(possible_translation_factor)_394020..394250_POSITIVE 361 104 64 202 155 aact:AA02939|5end_phosphoserine_phosphatase_2056881..2057111_POSITIVE 360 117 73 192 148 aact:AA00333|5end_1-phosphofructokinase;_tagatose-6-phosphate_kinase_225543..225773_POSITIVE 358 110 62 83 17 aact:AA01475|5end_conserved_hypothetical_protein_(possible_cytoplasmic_protein;_DNA_binding_protein)_990364..990594_POSITIVE 358 105 65 77 31 aact:AA00550|5end_ferredoxin-type_protein_376444..376674_POSITIVE 355 110 64 203 149 aact:AA01054|5end_conserved_hypothetical_protein_733678..733908_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 415 106 65 87 44 aact:AA00211|3end_hypothetical_protein_146163..146313_POSITIVE 396 110 62 47 13 aact:AA02366|3end_hypothetical_protein_1623252..1623402_POSITIVE 369 90 41 63 24 aact:AA02952|3end_lipoprotein_signal_peptidase_2065687..2065837_POSITIVE 365 104 65 87 31 aact:AA01269|3end_hypothetical_protein_863413..863563_POSITIVE 365 102 64 78 31 aact:AA00474|3end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_331724..331874_POSITIVE 358 110 62 70 4 aact:AA01474|3end_hypothetical_protein_990431..990581_POSITIVE 358 105 65 74 28 aact:AA00549|3end_ferredoxin-type_protein_376521..376671_POSITIVE 353 90 42 113 56 aact:AA02369|3end_conserved_hypothetical_protein_1624648..1624798_POSITIVE 353 100 51 145 104 aact:AA00409|3end_methionyl-tRNA_formyltransferase_274644..274794_POSITIVE 350 110 62 61 3 aact:AA01419|3end_D-ribulose-phosphate-3_epimerase_955129..955279_POSITIVE 342 110 62 151 95 aact:AA00737|3end_citrate_lyase,_alpha_chain_(citrase)_505143..505293_POSITIVE 341 89 42 89 39 aact:AA01954|3end_excinuclease_ABC,_subunit_C_1322314..1322464_POSITIVE 339 106 65 150 110 aact:AA01995|3end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355394..1355544_POSITIVE 335 105 66 79 42 aact:AA01753|3end_conserved_hypothetical_protein_1185902..1186052_POSITIVE 333 110 65 97 54 aact:AA02697|3end_hydrogenase_3_maturation_protease_1880408..1880558_POSITIVE 332 110 61 129 67 aact:AA02014|3end_probable_hemolysin_1370977..1371127_POSITIVE 329 86 43 151 112 aact:AA02917|3end_cystathionine_gamma-synthase_2043280..2043430_POSITIVE 328 110 62 62 25 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 327 90 42 117 69 aact:AA00475|3end_conserved_hypothetical_protein_(possible_N6-adenine-specific_methylase)_332282..332432_POSITIVE 322 97 62 150 116 aact:AA00241|3end_formate_acetyltransferase_activating_enzyme_(anaerobic_ribonucleoside-triphosphate_reductase_activating_protein)_172090..172240_POSITIVE