Origin IGS: taagccgcttttccctttttttaagaatttttcttaacgaaaagttagcagactccgcctaacgcatcgctggcgtaactttcctaaaaaatccttaaaaaaatgggcgtcttacacagggataaataatcaacaaaaaccgactacacttttatcatgccctagcggagtgctttcttttgcctacttttcttttcacaagcaaagacaaaatccaaggattttgaacgttggctttgccaatggtacgaagtgcaagcgcagcgcagtaaaagtaggtcgatgccgaaacaaaccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggtttgtttcggcatcgacctacttttactgcgctgcgcttgcacttcgtaccattggcaaagccaacgttcaaaatccttggattttgtctttgcttgtgaaaagaaaagtaggcaaaagaaagcactccgctagggcatgataaaagtgtagtcggtttttgttgattatttatccctgtgtaagacgcccatttttttaaggattttttaggaaagttacgccagcgatgcgttaggcggagtctgctaacttttcgttaagaaaaattcttaaaaaaagggaaaagcggctta Mask Tandem Repeat Region ================================================ taagccgcttttccctttttttaagaatttttcttaacgaaaagttagcagactccgcctaacgcatcgctggcgtaactttcctaaaaaatccttaaaaaaatgggcgtcttacacagggataaataatcaacaaaaaccgactacacttttatcatgccctagcggagtgctttcttttgcctacttttcttttcacaagcaaagacaaaatccaaggattttgaacgttggctttgccaatggtacgaagtgcaagcgcagcgcagtaaaagtaggtcgatgccgaaacaaaccta Find is-nt database================================================ Query_seq: AA02727:AA02728|AA02727:AA02728:hypothetical protein:conserved hypothetical protein (possible ATPase):->->:1909986..1910284 299 taagccgcttttccctttttttaagaatttttcttaacgaaaagttagcagactccgcctaacgcatcgctggcgtaactttcctaaaaaatccttaaaaaaatgggcgtcttacacagggataaataatcaacaaaaaccgactacacttttatcatgccctagcggagtgctttcttttgcctacttttcttttcacaagcaaagacaaaatccaaggattttgaacgttggctttgccaatggtacgaagtgcaagcgcagcgcagtaaaagtaggtcgatgccgaaacaaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02727:AA02728|AA02727:AA02728:hypothetical protein:conserved hypothetical protein (possible ATPase):->->:1909986..1910284 299 taagccgcttttccctttttttaagaatttttcttaacgaaaagttagcagactccgcctaacgcatcgctggcgtaactttcctaaaaaatccttaaaaaaatgggcgtcttacacagggataaataatcaacaaaaaccgactacacttttatcatgccctagcggagtgctttcttttgcctacttttcttttcacaagcaaagacaaaatccaaggattttgaacgttggctttgccaatggtacgaagtgcaagcgcagcgcagtaaaagtaggtcgatgccgaaacaaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02727:AA02728|AA02727:AA02728:hypothetical protein:conserved hypothetical protein (possible ATPase):->->:1909986..1910284 299 taagccgcttttccctttttttaagaatttttcttaacgaaaagttagcagactccgcctaacgcatcgctggcgtaactttcctaaaaaatccttaaaaaaatgggcgtcttacacagggataaataatcaacaaaaaccgactacacttttatcatgccctagcggagtgctttcttttgcctacttttcttttcacaagcaaagacaaaatccaaggattttgaacgttggctttgccaatggtacgaagtgcaagcgcagcgcagtaaaagtaggtcgatgccgaaacaaaccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagccgcttttccctttttttaagaatttttcttaacgaaaagttagcagactccgcctaacgcatcgctggcgtaactttcctaaaaaatccttaaaaaaatgggcgtcttacacagggataaataatcaacaaaaaccgactacacttttatcatgccctagcggagtgctttcttttgcctacttttcttttcacaagcaaagacaaaatccaaggattttgaacgttggctttgccaatggtacgaagtgcaagcgcagcgcagtaaaagtaggtcgatgccgaaacaaaccta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 126 End: 224 ............................................................................................................................. M I N K N R L H F Y H A L A E C F L L P T F L F T S K D K I Q G F ........................................................................... Protein_Len: 59 Strand: + Start: 122 End: 298 ......................................................................................................................... M N N Q Q K P T T L L S C P S G V L S F A Y F S F H K Q R Q N P R I L N V G F A N G T K C K R S A V K V G R C R N K P . Protein_Len: 32 Strand: - Start: 199 End: 294 ...................................................................................................................................................................................................... L C L C F G L I K F T P K A L P V F H L R L A T F T P R H R F M ..... Protein_Len: 40 Strand: - Start: 165 End: 284 .................................................................................................................................................................... R L A K K Q R S K K E C A F V F D L S K S R Q S Q W H Y S T C A C R L L L L D M ............... taagccgcttttccctttttttaagaatttttcttaacgaaaagttagcagactccgcctaacgcatcgctggcgtaactttcctaaaaaatccttaaaaaaatgggcgtcttacacagggataaataatcaacaaaaaccgactacacttttatcatgccctagcggagtgctttcttttgcctacttttcttttcacaagcaaagacaaaatccaaggattttgaacgttggctttgccaatggtacgaagtgcaagcgcagcgcagtaaaagtaggtcgatgccgaaacaaaccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagccgcttttccctttttttaagaatttttcttaacgaaaagttagcagactccgcctaacgcatcgctggcgtaactttcctaaaaaatccttaaaaaaatgggcgtcttacacagggataaataatcaacaaaaaccgactacacttttatcatgccctagcggagtgctttcttttgcctacttttcttttcacaagcaaagacaaaatccaaggattttgaacgttggctttgccaatggtacgaagtgcaagcgcagcgcagtaaaagtaggtcgatgccgaaacaaaccta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02727:AA02728|AA02727:AA02728:hypothetical protein:conserved hypothetical protein (possible ATPase):->->:1909986..1910284 299 taagccgcttttccctttttttaagaatttttcttaacgaaaagttagcagactccgcctaacgcatcgctggcgtaactttcctaaaaaatccttaaaaaaatgggcgtcttacacagggataaataatcaacaaaaaccgactacacttttatcatgccctagcggagtgctttcttttgcctacttttcttttcacaagcaaagacaaaatccaaggattttgaacgttggctttgccaatggtacgaagtgcaagcgcagcgcagtaaaagtaggtcgatgccgaaacaaaccta Intra-Species Hit: Count: 1 Min: 1 Max: 299 Len: 299 Subject: aact_AA02727_AA02728|hypothetical protein:conserved hypothetical protein (possible ATPase)|POSITIVE:POSITIVE|[1909986,1910284]|299 HSP 1 e-value: 1.0E-143 bit: 509.0 Len: 299 Query Start:1 Query End:299 Subject Strand: POSITIVE Subject Start: 1 Subject End: 299 taagccgcttttcccnnnnnnnaagaatttttcttaacgaaaagttagcagactccgcctaacgcatcgctggcgtaactttcctaaaaaatccttnnnnnnntgggcgtcttacacagggataaataatcaacaaaaaccgactacacttttatcatgccctagcggagtgctttcttttgcctacttttcttttcacaagcaaagacaaaatccaaggattttgaacgttggctttgccaatggtacgaagtgcaagcgcagcgcagtaaaagtaggtcgatgccgaaacaaaccta taagccgcttttccctttttttaagaatttttcttaacgaaaagttagcagactccgcctaacgcatcgctggcgtaactttcctaaaaaatccttaaaaaaatgggcgtcttacacagggataaataatcaacaaaaaccgactacacttttatcatgccctagcggagtgctttcttttgcctacttttcttttcacaagcaaagacaaaatccaaggattttgaacgttggctttgccaatggtacgaagtgcaagcgcagcgcagtaaaagtaggtcgatgccgaaacaaaccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGCCGCUUUUCCCUUUUUUUAAGAAUUUUUCUUAACGAAAAGUUAGCAGACUCCGCCUAACGCAUCGCUGGCGUAACUUUCCUAAAAAAUCCUUAAAAAAAUGGGCGUCUUACACAGGGAUAAAUAAUCAACAAAAACCGACUACACUUUUAUCAUGCCCUAGCGGAGUGCUUUCUUUUGCCUACUUUUCUUUUCACAAGCAAAGACAAAAUCCAAGGAUUUUGAACGUUGGCUUUGCCAAUGGUACGAAGUGCAAGCGCAGCGCAGUAAAAGUAGGUCGAUGCCGAAACAAACCUA ((((.(((....((.((((((((((.((((((.(((((.....))))).......((((............))))...........)))))).)))))))))).))))).))))...((((((.....))).........(((((..((((((((..(((.((.(((...(((...((((((((..((((.(((.....))).)))).(((((((....)))))))...((((((...)))))))))).)))).)))..))))).))))))))))).))))).............))). (-69.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGUUUGUUUCGGCAUCGACCUACUUUUACUGCGCUGCGCUUGCACUUCGUACCAUUGGCAAAGCCAACGUUCAAAAUCCUUGGAUUUUGUCUUUGCUUGUGAAAAGAAAAGUAGGCAAAAGAAAGCACUCCGCUAGGGCAUGAUAAAAGUGUAGUCGGUUUUUGUUGAUUAUUUAUCCCUGUGUAAGACGCCCAUUUUUUUAAGGAUUUUUUAGGAAAGUUACGCCAGCGAUGCGUUAGGCGGAGUCUGCUAACUUUUCGUUAAGAAAAAUUCUUAAAAAAAGGGAAAAGCGGCUUA ((((..((..(((((((((((.((((((((((((.((..((((((..(((....(((.(((((((.(((.((((((.....)))))).))).))))))).)))....)))..)))))).......(((.....))))).))).).))))))))..)))))....)))))).....))..))))..((((.(((((.((((((((((((((((((.(((((((((.((..((..(((.....)))..))..))))))))))).....)))))))))))))))))).))....))).)))) (-91.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 576 260 211 197 140 aact:AA02108|5end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1433393..1433623_POSITIVE 473 270 223 53 1 aact:AA02106|5end_hypothetical_protein_1433241..1433471_POSITIVE 408 208 164 229 181 aact:AA02489|5end_hypothetical_protein_1726454..1726684_POSITIVE 408 208 164 52 4 aact:AA02487|5end_hypothetical_protein_1726277..1726507_POSITIVE 395 264 222 158 119 aact:AA01633|5end_probable_ferredoxin-like_protein_1104008..1104238_POSITIVE 394 77 38 223 182 aact:AA00461|5end_heat_shock_protein;_protease_322375..322605_POSITIVE 394 264 223 226 188 aact:AA00200|5end_thymidylate_synthase_138892..139122_POSITIVE 369 264 222 140 89 aact:AA01098|5end_bis(5'-nucleosyl)-tetraphosphatase_757699..757929_POSITIVE 362 76 48 69 33 aact:AA00603|5end_undecaprenyl_pyrophosphate_synthetase_(UPPS)_410283..410513_POSITIVE 356 80 38 108 66 aact:AA00989|5end_hypothetical_protein_692319..692549_POSITIVE 348 94 55 212 168 aact:AA01998|5end_16S_rRNA_pseudouridine_synthase_1356842..1357072_POSITIVE 344 288 247 56 10 aact:AA02933|5end_conserved_hypothetical_protein_(HesA/MoeB/ThiF_family_protein)_2053467..2053697_POSITIVE 344 270 229 82 42 aact:AA02733|5end_cell_division_protein_K;_sporulation_protein_spoIIIE_1912208..1912438_POSITIVE 342 266 228 109 63 aact:AA01426|5end_threonine_deaminase_(dehydratase)_958207..958437_POSITIVE 341 46 1 71 27 aact:AA00658|5end_hypothetical_protein_451618..451848_POSITIVE 333 267 223 177 142 aact:AA00991|5end_conserved_hypothetical_protein_693191..693421_POSITIVE 332 270 225 176 123 aact:AA00866|5end_conserved_hypothetical_protein_599925..600155_POSITIVE 330 176 136 109 62 aact:AA01986|5end_pyruvate_dehydrogenase_complex_E2_1348753..1348983_POSITIVE 326 86 52 199 152 aact:AA01146|5end_conserved_hypothetical_protein_784433..784663_POSITIVE 324 84 49 53 17 aact:AA01861|5end_phosphomethylpyrimidine_kinase_1251806..1252036_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 451 239 207 151 118 aact:AA02106|3end_hypothetical_protein_1433455..1433605_POSITIVE 415 178 134 151 102 aact:AA02184|3end_anthranilate_isomerase;_indole-3-glycerol_phosphate_synthase;_phosphoribosylanthranilate_isomerase_1492541..1492691_POSITIVE 394 76 36 151 98 aact:AA00396|3end_conserved_hypothetical_protein_265952..266102_POSITIVE 369 269 229 62 15 aact:AA01167|3end_glucose-6-phosphate_1-dehydrogenase_801067..801217_POSITIVE 344 288 247 53 7 aact:AA02932|3end_lytic_murein_transglycosylase_A_2053544..2053694_POSITIVE 342 266 228 55 9 aact:AA01424|3end_threonine_deaminase_(dehydratase)_958233..958383_POSITIVE 334 266 229 140 101 aact:AA00218|3end_argininosuccinate_lyase_152211..152361_POSITIVE 330 176 136 77 30 aact:AA01985|3end_pyruvate_dehydrogenase,_E1_component_1348801..1348951_POSITIVE 326 262 222 68 18 aact:AA01342|3end_conserved_hypotheticl_protein_(possible_ribosomal_RNA_adenine_dimethylase)_911085..911235_POSITIVE 326 86 52 146 99 aact:AA01145|3end_glycyl-tRNA_synthetase_alpha_subunit_784460..784610_POSITIVE 324 84 49 57 21 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 321 270 228 139 103 aact:AA01344|3end_magnesium_and_cobalt_transport_protein_912685..912835_POSITIVE 319 275 232 148 92 aact:AA01550|3end_hypothetical_protein_1038292..1038442_POSITIVE 318 270 222 145 89 aact:AA02167|3end_hypothetical_protein_1483474..1483624_POSITIVE 315 289 244 148 108 aact:AA00865|3end_conserved_hypothetical_protein_600014..600164_POSITIVE 315 290 254 105 66 aact:AA00179|3end_conserved_hypothetical_protein_(possible_outer_membrane_protein)_127725..127875_POSITIVE 313 289 244 140 99 aact:AA02686|3end_hydrogenase_4_component_C_1872585..1872735_POSITIVE 313 264 228 46 1 aact:AA01097|3end_conserved_hypothetical_protein_757685..757835_POSITIVE 309 267 227 61 7 aact:AA01744|3end_peptide_chain_release_factor_RF-2_1180352..1180502_POSITIVE 308 277 231 73 28 aact:AA01875|3end_PTS_system,_glucose-specific_enzyme_II,_A_component_1263506..1263656_POSITIVE