Origin IGS: aagttgttacctttattctattgctaataccagttgaaaacggtgtttattctatatcgtattaacaaaatagactagcaaaaacatggactaaagtgcggttgtttttatcaatgtttttctccgccgctaaaaatttttagccaaaaagcttaagcgatttagtgtgccaaacgctgaattcttaatgtcaggaattttgatttatcgcaacaatctacataattctactacttaagaagtagccttaatgttagactaatgccgatttttcaatcataaaatgacattaatttccggagtatct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agatactccggaaattaatgtcattttatgattgaaaaatcggcattagtctaacattaaggctacttcttaagtagtagaattatgtagattgttgcgataaatcaaaattcctgacattaagaattcagcgtttggcacactaaatcgcttaagctttttggctaaaaatttttagcggcggagaaaaacattgataaaaacaaccgcactttagtccatgtttttgctagtctattttgttaatacgatatagaataaacaccgttttcaactggtattagcaatagaataaaggtaacaactt Mask Tandem Repeat Region ================================================ aagttgttacctttattctattgctaataccagttgaaaacggtgtttattctatatcgtattaacaaaatagactagcaaaaacatggactaaagtgcggttgtttttatcaatgtttttctccgccgctaaaaatttttagccaaaaagcttaagcgatttagtgtgccaaacgctgaattcttaatgtcaggaattttgatttatcgcaacaatctacataattctactacttaagaagtagccttaatgttagactaatgccgatttttcaatcataaaatgacattaatttccggagtatct Find is-nt database================================================ Query_seq: AA02724:AA02726|AA02724:AA02726:seryl-tRNA ligase:anaerobic C4-dicarboxylate transporter:->->:1907811..1908117 307 aagttgttacctttattctattgctaataccagttgaaaacggtgtttattctatatcgtattaacaaaatagactagcaaaaacatggactaaagtgcggttgtttttatcaatgtttttctccgccgctaaaaatttttagccaaaaagcttaagcgatttagtgtgccaaacgctgaattcttaatgtcaggaattttgatttatcgcaacaatctacataattctactacttaagaagtagccttaatgttagactaatgccgatttttcaatcataaaatgacattaatttccggagtatct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02724:AA02726|AA02724:AA02726:seryl-tRNA ligase:anaerobic C4-dicarboxylate transporter:->->:1907811..1908117 307 aagttgttacctttattctattgctaataccagttgaaaacggtgtttattctatatcgtattaacaaaatagactagcaaaaacatggactaaagtgcggttgtttttatcaatgtttttctccgccgctaaaaatttttagccaaaaagcttaagcgatttagtgtgccaaacgctgaattcttaatgtcaggaattttgatttatcgcaacaatctacataattctactacttaagaagtagccttaatgttagactaatgccgatttttcaatcataaaatgacattaatttccggagtatct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02724:AA02726|AA02724:AA02726:seryl-tRNA ligase:anaerobic C4-dicarboxylate transporter:->->:1907811..1908117 307 aagttgttacctttattctattgctaataccagttgaaaacggtgtttattctatatcgtattaacaaaatagactagcaaaaacatggactaaagtgcggttgtttttatcaatgtttttctccgccgctaaaaatttttagccaaaaagcttaagcgatttagtgtgccaaacgctgaattcttaatgtcaggaattttgatttatcgcaacaatctacataattctactacttaagaagtagccttaatgttagactaatgccgatttttcaatcataaaatgacattaatttccggagtatct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aagttgttacctttattctattgctaataccagttgaaaacggtgtttattctatatcgtattaacaaaatagactagcaaaaacatggactaaagtgcggttgtttttatcaatgtttttctccgccgctaaaaatttttagccaaaaagcttaagcgatttagtgtgccaaacgctgaattcttaatgtcaggaattttgatttatcgcaacaatctacataattctactacttaagaagtagccttaatgttagactaatgccgatttttcaatcataaaatgacattaatttccggagtatct Predict ORF larger than 30AA ================================================ aagttgttacctttattctattgctaataccagttgaaaacggtgtttattctatatcgtattaacaaaatagactagcaaaaacatggactaaagtgcggttgtttttatcaatgtttttctccgccgctaaaaatttttagccaaaaagcttaagcgatttagtgtgccaaacgctgaattcttaatgtcaggaattttgatttatcgcaacaatctacataattctactacttaagaagtagccttaatgttagactaatgccgatttttcaatcataaaatgacattaatttccggagtatct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aagttgttacctttattctattgctaataccagttgaaaacggtgtttattctatatcgtattaacaaaatagactagcaaaaacatggactaaagtgcggttgtttttatcaatgtttttctccgccgctaaaaatttttagccaaaaagcttaagcgatttagtgtgccaaacgctgaattcttaatgtcaggaattttgatttatcgcaacaatctacataattctactacttaagaagtagccttaatgttagactaatgccgatttttcaatcataaaatgacattaatttccggagtatct Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02724:AA02726|AA02724:AA02726:seryl-tRNA ligase:anaerobic C4-dicarboxylate transporter:->->:1907811..1908117 307 aagttgttacctttattctattgctaataccagttgaaaacggtgtttattctatatcgtattaacaaaatagactagcaaaaacatggactaaagtgcggttgtttttatcaatgtttttctccgccgctaaaaatttttagccaaaaagcttaagcgatttagtgtgccaaacgctgaattcttaatgtcaggaattttgatttatcgcaacaatctacataattctactacttaagaagtagccttaatgttagactaatgccgatttttcaatcataaaatgacattaatttccggagtatct Intra-Species Hit: Count: 1 Min: 1 Max: 307 Len: 307 Subject: aact_AA02724_AA02726|seryl-tRNA ligase:anaerobic C4-dicarboxylate transporter|POSITIVE:POSITIVE|[1907811,1908117]|307 HSP 1 e-value: 1.0E-173 bit: 609.0 Len: 307 Query Start:1 Query End:307 Subject Strand: POSITIVE Subject Start: 1 Subject End: 307 aagttgttacctttattctattgctaataccagttgaaaacggtgtttattctatatcgtattaacaaaatagactagcaaaaacatggactaaagtgcggttgtttttatcaatgtttttctccgccgctaaaaatttttagccaaaaagcttaagcgatttagtgtgccaaacgctgaattcttaatgtcaggaattttgatttatcgcaacaatctacataattctactacttaagaagtagccttaatgttagactaatgccgatttttcaatcataaaatgacattaatttccggagtatct aagttgttacctttattctattgctaataccagttgaaaacggtgtttattctatatcgtattaacaaaatagactagcaaaaacatggactaaagtgcggttgtttttatcaatgtttttctccgccgctaaaaatttttagccaaaaagcttaagcgatttagtgtgccaaacgctgaattcttaatgtcaggaattttgatttatcgcaacaatctacataattctactacttaagaagtagccttaatgttagactaatgccgatttttcaatcataaaatgacattaatttccggagtatct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGUUGUUACCUUUAUUCUAUUGCUAAUACCAGUUGAAAACGGUGUUUAUUCUAUAUCGUAUUAACAAAAUAGACUAGCAAAAACAUGGACUAAAGUGCGGUUGUUUUUAUCAAUGUUUUUCUCCGCCGCUAAAAAUUUUUAGCCAAAAAGCUUAAGCGAUUUAGUGUGCCAAACGCUGAAUUCUUAAUGUCAGGAAUUUUGAUUUAUCGCAACAAUCUACAUAAUUCUACUACUUAAGAAGUAGCCUUAAUGUUAGACUAAUGCCGAUUUUUCAAUCAUAAAAUGACAUUAAUUUCCGGAGUAUCU .((..((...(((((.((((((((((.((...(((((..(((((((.......))))))).)))))....))...))))).....))))).))))).))..))..................(((((..((((((....))))))..............((((((((((.....)))))))))).(((((((((......((((...((((((....(((((((((..(((((.(....).)))))..)).))).))))....)).))))...))))........)))))))))....)))))..... (-62.04) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAUACUCCGGAAAUUAAUGUCAUUUUAUGAUUGAAAAAUCGGCAUUAGUCUAACAUUAAGGCUACUUCUUAAGUAGUAGAAUUAUGUAGAUUGUUGCGAUAAAUCAAAAUUCCUGACAUUAAGAAUUCAGCGUUUGGCACACUAAAUCGCUUAAGCUUUUUGGCUAAAAAUUUUUAGCGGCGGAGAAAAACAUUGAUAAAAACAACCGCACUUUAGUCCAUGUUUUUGCUAGUCUAUUUUGUUAAUACGAUAUAGAAUAAACACCGUUUUCAACUGGUAUUAGCAAUAGAAUAAAGGUAACAACUU .....(((((....(((((((((......((((....)))).(((.(((((((.(((.((..(((((........)))))..)))))))))))).)))................)))))))))......(((((((((....))))).))))...........((((((....))))))..)))))......................((.(((((.((..((((..(((((((((((.((((....)))).)))))..((((...))))....))))))..))))...)).)))))))........ (-68.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 417 133 93 183 144 aact:AA02509|5end_chorismate_synthase_1740952..1741182_POSITIVE 392 130 88 182 143 aact:AA00569|5end_ketol-acid_reductoisomerase_391191..391421_POSITIVE 376 130 83 69 4 aact:AA01174|5end_hypothetical_protein_804700..804930_POSITIVE 355 121 86 121 82 aact:AA00837|5end_cell_division_protein_W_576317..576547_POSITIVE 350 127 87 176 135 aact:AA00084|5end_aminotransferase_60814..61044_POSITIVE 348 131 91 192 151 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 346 129 87 148 97 aact:AA01954|5end_excinuclease_ABC,_subunit_C_1320468..1320698_POSITIVE 345 128 93 154 121 aact:AA02885|5end_conserved_hypothetical_protein_2022428..2022658_POSITIVE 345 131 93 198 160 aact:AA02162|5end_lipoprotein_1478950..1479180_POSITIVE 345 125 88 126 89 aact:AA01286|5end_5,10-methylenetetrahydrofolate_dehydrogenase_(bifunctional_protein)_870309..870539_POSITIVE 341 130 87 162 112 aact:AA00356|5end_DNA-directed_RNA_polymerase,_beta'_chain_238937..239167_POSITIVE 335 127 86 152 109 aact:AA02757|5end_histidinol-phosphate_aminotransferase_2_1930634..1930864_POSITIVE 329 130 89 42 1 aact:AA01798|5end_hypothetical_protein_1212070..1212300_POSITIVE 327 130 93 154 114 aact:AA00766|5end_heat_shock_protein_B25.3_(HSP-70_cofactor)_527384..527614_POSITIVE 327 126 86 175 136 aact:AA00292|5end_dihydrofolate_reductase_201800..202030_POSITIVE 327 126 86 90 51 aact:AA00291|5end_hypothetical_protein_201715..201945_POSITIVE 326 121 85 198 158 aact:AA01793|5end_conserved_hypothetical_protein_1208627..1208857_POSITIVE 323 130 86 140 77 aact:AA02034|5end_ferredoxin_NADP+_reductase_1385198..1385428_POSITIVE 322 130 93 151 111 aact:AA02860|5end_conserved_hypothetical_protein_2006074..2006304_POSITIVE 322 127 87 169 129 aact:AA00987|5end_tetracenomycin_polyketide_synthesis_O-methyltransferase_689745..689975_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 357 131 93 103 67 aact:AA02208|3end_tryptophan_synthase,__alpha_subunit_1503996..1504146_POSITIVE 355 121 86 77 38 aact:AA00835|3end_UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapep_tide)_576353..576503_POSITIVE 350 127 87 133 92 aact:AA00083|3end_hypothetical_protein_60851..61001_POSITIVE 350 127 87 49 8 aact:AA00081|3end_fatty_acid_metabolism_regulator_protein_60767..60917_POSITIVE 348 131 91 109 68 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 345 128 93 73 40 aact:AA02883|3end_conserved_hypothetical_protein_2022427..2022577_POSITIVE 345 131 93 97 59 aact:AA02160|3end_conserved_hypothetical_protein_1478929..1479079_POSITIVE 341 130 87 56 6 aact:AA00355|3end_DNA-directed_RNA_polymerase_beta_chain_(transcriptase_beta_chain)_238911..239061_POSITIVE 339 130 87 60 13 aact:AA01796|3end_branched-chain_amino_acid_aminotransferase_1212168..1212318_POSITIVE 335 127 86 74 31 aact:AA02756|3end_phosphoserine_aminotransferase_1930636..1930786_POSITIVE 327 126 86 108 69 aact:AA00290|3end_conserved_hypothetical_protein_201813..201963_POSITIVE 326 121 85 78 38 aact:AA01792|3end_conserved_hypothetical_protein_1208587..1208737_POSITIVE 325 130 92 123 72 aact:AA02253|3end_hypothetical_protein_1534786..1534936_POSITIVE 322 130 93 65 25 aact:AA02858|3end_conserved_hypothetical_protein_2006068..2006218_POSITIVE 322 127 87 85 45 aact:AA00985|3end_transcriptional_regulator_(LysR_family)_689741..689891_POSITIVE 321 128 92 44 8 aact:AA01843|3end_sensor_protein;_probable_two-component_system_sensor_histidine_kinase_1239830..1239980_POSITIVE 317 127 85 45 3 aact:AA02992|3end_RNA_methyltransferase_2091740..2091890_POSITIVE 315 131 93 101 51 aact:AA01718|3end_DNA_polymerase_III,_chi_subunit_1163316..1163466_POSITIVE 314 136 92 128 87 aact:AA01917|3end_hypothetical_protein_1290996..1291146_POSITIVE 312 135 93 107 58 aact:AA01280|3end_groES_chaperonin_868530..868680_POSITIVE