Origin IGS: taagttattggcttacattctcccgtaggggcgggtcctgtgcccgcccgaatttacgtttggataatgggcgggcacaggacccgcccctacaaaagcaactctgttgcttagatgtaaagcagtaaggacgtatc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gatacgtccttactgctttacatctaagcaacagagttgcttttgtaggggcgggtcctgtgcccgcccattatccaaacgtaaattcgggcgggcacaggacccgcccctacgggagaatgtaagccaataactta Mask Tandem Repeat Region ================================================ taagttattggcttacattctcccgtaggggcgggtcctgtgcccgcccgaatttacgtttggataatgggcgggcacaggacccgcccctacaaaagcaactctgttgcttagatgtaaagcagtaaggacgtatc Find is-nt database================================================ Query_seq: AA02695:AA02696|AA02695:AA02696:hydrogenase-3 component G:formate hydrogenlyase maturation protein:->->:1879479..1879615 137 taagttattggcttacattctcccgtaggggcgggtcctgtgcccgcccgaatttacgtttggataatgggcgggcacaggacccgcccctacaaaagcaactctgttgcttagatgtaaagcagtaaggacgtatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02695:AA02696|AA02695:AA02696:hydrogenase-3 component G:formate hydrogenlyase maturation protein:->->:1879479..1879615 137 taagttattggcttacattctcccgtaggggcgggtcctgtgcccgcccgaatttacgtttggataatgggcgggcacaggacccgcccctacaaaagcaactctgttgcttagatgtaaagcagtaaggacgtatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02695:AA02696|AA02695:AA02696:hydrogenase-3 component G:formate hydrogenlyase maturation protein:->->:1879479..1879615 137 taagttattggcttacattctcccgtaggggcgggtcctgtgcccgcccgaatttacgtttggataatgggcgggcacaggacccgcccctacaaaagcaactctgttgcttagatgtaaagcagtaaggacgtatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagttattggcttacattctcccgtaggggcgggtcctgtgcccgcccgaatttacgtttggataatgggcgggcacaggacccgcccctacaaaagcaactctgttgcttagatgtaaagcagtaaggacgtatc Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 7 End: 111 ...... M G L H S P V G A G P V P A R I Y V W I M G G H R T R P Y K S N S V A .......................... Protein_Len: 43 Strand: - Start: 8 End: 136 ....... Q S V N E R L P P D Q A R G F K R K S L P R A C S G G R C F C S Q Q K S T F C Y P R M . taagttattggcttacattctcccgtaggggcgggtcctgtgcccgcccgaatttacgtttggataatgggcgggcacaggacccgcccctacaaaagcaactctgttgcttagatgtaaagcagtaaggacgtatc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagttattggcttacattctcccgtaggggcgggtcctgtgcccgcccgaatttacgtttggataatgggcgggcacaggacccgcccctacaaaagcaactctgttgcttagatgtaaagcagtaaggacgtatc Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 72 HP_score: -11.8 Tail_Score: -2.57564 Start: 69 End: 89 Full_Region: gagttgcttttgtag gggcgggt cctgt gcccgccc attatccaaacgtaa ....................................................................gggcgggtcctgtgcccgccc................................................ Find igs database================================================ Query_seq: AA02695:AA02696|AA02695:AA02696:hydrogenase-3 component G:formate hydrogenlyase maturation protein:->->:1879479..1879615 137 taagttattggcttacattctcccgtaggggcgggtcctgtgcccgcccgaatttacgtttggataatgggcgggcacaggacccgcccctacaaaagcaactctgttgcttagatgtaaagcagtaaggacgtatc Intra-Species Hit: Count: 1 Min: 1 Max: 137 Len: 137 Subject: aact_AA02695_AA02696|hydrogenase-3 component G:formate hydrogenlyase maturation protein|POSITIVE:POSITIVE|[1879479,1879615]|137 HSP 1 e-value: 3.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 1 Subject End: 137 taagttattggcttacattctcccgtaggggcgggtcctgtgcccgcccgaatttacgtttggataatgggcgggcacaggacccgcccctacaaaagcaactctgttgcttagatgtaaagcagtaaggacgtatc taagttattggcttacattctcccgtaggggcgggtcctgtgcccgcccgaatttacgtttggataatgggcgggcacaggacccgcccctacaaaagcaactctgttgcttagatgtaaagcagtaaggacgtatc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGUUAUUGGCUUACAUUCUCCCGUAGGGGCGGGUCCUGUGCCCGCCCGAAUUUACGUUUGGAUAAUGGGCGGGCACAGGACCCGCCCCUACAAAAGCAACUCUGUUGCUUAGAUGUAAAGCAGUAAGGACGUAUC ....((((((..(((((((.....((((((((((((((((((((((((((.(((((....)))))..))))))))))))))))))))))))))..(((((((...))))))).)))))))..))))))......... (-80.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUACGUCCUUACUGCUUUACAUCUAAGCAACAGAGUUGCUUUUGUAGGGGCGGGUCCUGUGCCCGCCCAUUAUCCAAACGUAAAUUCGGGCGGGCACAGGACCCGCCCCUACGGGAGAAUGUAAGCCAAUAACUUA ........(((((.((((.......))))..........((((((((((((((((((((((((((((((...................))))))))))))))))))))))))))))))...)))))........... (-74.91) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 478 49 21 127 98 aact:AA01635|5end_ribonucleoside_diphosphate_reductase,_beta_chain_1104615..1104845_POSITIVE 440 76 33 128 77 aact:AA02433|5end_hypothetical_protein_1678300..1678530_POSITIVE 429 60 24 190 146 aact:AA02014|5end_probable_hemolysin_1370406..1370636_POSITIVE 425 64 21 54 9 aact:AA00132|5end_conserved_hypothetical_protein_(possible_regulator_protein,_VirR_family)_94938..95168_POSITIVE 407 90 41 74 22 aact:AA00634|5end_DNA_polymerase_IV_(Pol_IV)_435182..435412_POSITIVE 403 87 43 66 13 aact:AA02954|5end_penicillin_tolerance_protein;_hydroxymethylbutenyl_pyrophosphate_reductase_2065610..2065840_POSITIVE 393 50 16 119 77 aact:AA01260|5end_hypothetical_protein_855550..855780_POSITIVE 390 76 31 226 188 aact:AA02158|5end_TonB-dependent_outer_membrane_receptor_1476155..1476385_POSITIVE 385 77 31 128 81 aact:AA01724|5end_valyl-tRNA_synthetase_1164446..1164676_POSITIVE 383 89 41 56 7 aact:AA01422|5end_cell_division_protein_E,_ABC_system_ATP-binding_protein_956009..956239_POSITIVE 380 90 43 215 158 aact:AA00200|5end_thymidylate_synthase_138892..139122_POSITIVE 370 69 24 87 27 aact:AA02099|5end_50S_ribosomal_protein_L25_1429639..1429869_POSITIVE 366 90 44 231 173 aact:AA02934|5end_hypothetical_protein_2054242..2054472_POSITIVE 363 90 44 135 84 aact:AA00525|5end_hypothetical_protein_359254..359484_POSITIVE 363 75 31 70 25 aact:AA00488|5end_purine_nucleoside_phosphorylase_338075..338305_POSITIVE 362 72 32 46 12 aact:AA02775|5end_S-adenosylmethionine:tRNA_ribosyltransferase-isomerase_(Queuosine_biosynthesis_protein_queA)_1938555..1938785_POSITIVE 361 75 32 69 30 aact:AA02421|5end_conserved_hypothetical_protein_1667044..1667274_POSITIVE 361 75 32 75 36 aact:AA02297|5end_conserved_hypothetical_protein_1569620..1569850_POSITIVE 361 75 32 75 36 aact:AA00621|5end_conserved_hypothetical_protein_428727..428957_POSITIVE 361 75 32 75 36 aact:AA00412|5end_conserved_hypothetical_protein_279174..279404_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 786 90 41 63 14 aact:AA02695|3end_hydrogenase-3_component_G_1879418..1879568_POSITIVE 478 49 21 102 73 aact:AA01634|3end_hypothetical_protein_1104670..1104820_POSITIVE 440 76 33 73 22 aact:AA02432|3end_penicillin-binding_protein_1B_1678325..1678475_POSITIVE 429 60 24 78 34 aact:AA02013|3end_carbonic_anhydrase_1370374..1370524_POSITIVE 403 87 43 63 10 aact:AA02952|3end_lipoprotein_signal_peptidase_2065687..2065837_POSITIVE 393 50 16 90 48 aact:AA01565|3end_conserved_hypothetical_protein_1050967..1051117_POSITIVE 360 76 36 151 101 aact:AA01956|3end_3-deoxy-manno-octulosonate_cytidylyltransferase_1323286..1323436_POSITIVE 356 80 34 51 1 aact:AA02844|3end_Holliday_junction_DNA_helicase_1997885..1998035_POSITIVE 351 50 2 141 85 aact:AA00760|3end_conserved_hypothetical_protein_(possible_peptidase)_523815..523965_POSITIVE 350 50 7 88 45 aact:AA02543|3end_conserved_hypothetical_protein_1773434..1773584_POSITIVE 347 74 34 50 4 aact:AA02031|3end_type_III_site-specific_deoxyribonuclease_1385155..1385305_POSITIVE 347 76 35 53 1 aact:AA01372|3end_ribonuclease_r,_virulence_associated_protein_930363..930513_POSITIVE 347 90 41 55 5 aact:AA01201|3end_phosphatase;_hydrolase_823303..823453_POSITIVE 337 110 69 65 23 aact:AA00576|3end_conserved_hypothetical_protein_(possible_translation_factor)_394648..394798_POSITIVE 335 87 43 51 4 aact:AA02106|3end_hypothetical_protein_1433455..1433605_POSITIVE 335 82 45 55 4 aact:AA01625|3end_riboflavin_synthase,_beta_chain_1100514..1100664_POSITIVE 333 90 41 72 14 aact:AA00789|3end_DNA_topoisomerase_III_542545..542695_POSITIVE 331 98 58 150 103 aact:AA02096|3end_hypothetical_protein_1428956..1429106_POSITIVE 331 50 17 54 17 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 331 90 44 56 11 aact:AA00807|3end_fumarate_reductase_13_kD_hydrophobic_protein_553188..553338_POSITIVE