Origin IGS: taaaccggaaaagtgaaaagaaacgctaatcaaaacatggcttgccatattgcaacaagcttactcaagctcaattttactgccatggagctgaaagataattagggcaattaaaggaaacggatttaccgattatacagactaaccaaggttgtctatcggcttgcccgttggcgtgagatgttaattcgcgatgaggttcactttatagcatacagcgcataagctgatgggaaaatttatctgcccaagttttgagaggcatgtgttaataaataatattttttgctccatagcattcatttctttccatggcatattgtttctccgagagattttgacactcattttacaagattgaaatataaacgatgttttgaacgcttcatgtgtgaaccaccgtattgcatacaactgtaatattttgaacta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagttcaaaatattacagttgtatgcaatacggtggttcacacatgaagcgttcaaaacatcgtttatatttcaatcttgtaaaatgagtgtcaaaatctctcggagaaacaatatgccatggaaagaaatgaatgctatggagcaaaaaatattatttattaacacatgcctctcaaaacttgggcagataaattttcccatcagcttatgcgctgtatgctataaagtgaacctcatcgcgaattaacatctcacgccaacgggcaagccgatagacaaccttggttagtctgtataatcggtaaatccgtttcctttaattgccctaattatctttcagctccatggcagtaaaattgagcttgagtaagcttgttgcaatatggcaagccatgttttgattagcgtttcttttcacttttccggttta Mask Tandem Repeat Region ================================================ taaaccggaaaagtgaaaagaaacgctaatcaaaacatggcttgccatattgcaacaagcttactcaagctcaattttactgccatggagctgaaagataattagggcaattaaaggaaacggatttaccgattatacagactaaccaaggttgtctatcggcttgcccgttggcgtgagatgttaattcgcgatgaggttcactttatagcatacagcgcataagctgatgggaaaatttatctgcccaagttttgagaggcatgtgttaataaataatattttttgctccatagcattcatttctttccatggcatattgtttctccgagagattttgacactcattttacaagattgaaatataaacgatgttttgaacgcttcatgtgtgaaccaccgtattgcatacaactgtaatattttgaacta Find is-nt database================================================ Query_seq: AA02681:AA02682|AA02681:AA02682:hypothetical protein:hydrogenase maturation protein:->->:1866012..1866443 432 taaaccggaaaagtgaaaagaaacgctaatcaaaacatggcttgccatattgcaacaagcttactcaagctcaattttactgccatggagctgaaagataattagggcaattaaaggaaacggatttaccgattatacagactaaccaaggttgtctatcggcttgcccgttggcgtgagatgttaattcgcgatgaggttcactttatagcatacagcgcataagctgatgggaaaatttatctgcccaagttttgagaggcatgtgttaataaataatattttttgctccatagcattcatttctttccatggcatattgtttctccgagagattttgacactcattttacaagattgaaatataaacgatgttttgaacgcttcatgtgtgaaccaccgtattgcatacaactgtaatattttgaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02681:AA02682|AA02681:AA02682:hypothetical protein:hydrogenase maturation protein:->->:1866012..1866443 432 taaaccggaaaagtgaaaagaaacgctaatcaaaacatggcttgccatattgcaacaagcttactcaagctcaattttactgccatggagctgaaagataattagggcaattaaaggaaacggatttaccgattatacagactaaccaaggttgtctatcggcttgcccgttggcgtgagatgttaattcgcgatgaggttcactttatagcatacagcgcataagctgatgggaaaatttatctgcccaagttttgagaggcatgtgttaataaataatattttttgctccatagcattcatttctttccatggcatattgtttctccgagagattttgacactcattttacaagattgaaatataaacgatgttttgaacgcttcatgtgtgaaccaccgtattgcatacaactgtaatattttgaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02681:AA02682|AA02681:AA02682:hypothetical protein:hydrogenase maturation protein:->->:1866012..1866443 432 taaaccggaaaagtgaaaagaaacgctaatcaaaacatggcttgccatattgcaacaagcttactcaagctcaattttactgccatggagctgaaagataattagggcaattaaaggaaacggatttaccgattatacagactaaccaaggttgtctatcggcttgcccgttggcgtgagatgttaattcgcgatgaggttcactttatagcatacagcgcataagctgatgggaaaatttatctgcccaagttttgagaggcatgtgttaataaataatattttttgctccatagcattcatttctttccatggcatattgtttctccgagagattttgacactcattttacaagattgaaatataaacgatgttttgaacgcttcatgtgtgaaccaccgtattgcatacaactgtaatattttgaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaccggaaaagtgaaaagaaacgctaatcaaaacatggcttgccatattgcaacaagcttactcaagctcaattttactgccatggagctgaaagataattagggcaattaaaggaaacggatttaccgattatacagactaaccaaggttgtctatcggcttgcccgttggcgtgagatgttaattcgcgatgaggttcactttatagcatacagcgcataagctgatgggaaaatttatctgcccaagttttgagaggcatgtgttaataaataatattttttgctccatagcattcatttctttccatggcatattgtttctccgagagattttgacactcattttacaagattgaaatataaacgatgttttgaacgcttcatgtgtgaaccaccgtattgcatacaactgtaatattttgaacta Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 13 End: 102 ............ M K R N A N Q N M A C H I A T S L L K L N F T A M E L K D N .......................................................................................................................................................................................................................................................................................................................................... Protein_Len: 33 Strand: + Start: 124 End: 222 ........................................................................................................................... M Y R L Y R L T K V V Y R L A R W R E M L I R D E V H F I A Y S A .................................................................................................................................................................................................................. Protein_Len: 44 Strand: + Start: 227 End: 358 .................................................................................................................................................................................................................................. M M G K F I C P S F E R H V L I N N I F C S I A F I S F H G I L F L R E I L T L I L Q D .......................................................................... Protein_Len: 44 Strand: + Start: 286 End: 417 ............................................................................................................................................................................................................................................................................................. M L H S I H F F P W H I V S P R D F D T H F T R L K Y K R C F E R F M C E P P Y C I Q L ............... Protein_Len: 60 Strand: - Start: 187 End: 423 .......................................................................................................................................................................................... Q G L K S L C T N I F L I K Q E M A N M E K W P M N N R R S I K V S M K C S Q F I F S T K F A E H T F W R I A Y L Q L M ......... taaaccggaaaagtgaaaagaaacgctaatcaaaacatggcttgccatattgcaacaagcttactcaagctcaattttactgccatggagctgaaagataattagggcaattaaaggaaacggatttaccgattatacagactaaccaaggttgtctatcggcttgcccgttggcgtgagatgttaattcgcgatgaggttcactttatagcatacagcgcataagctgatgggaaaatttatctgcccaagttttgagaggcatgtgttaataaataatattttttgctccatagcattcatttctttccatggcatattgtttctccgagagattttgacactcattttacaagattgaaatataaacgatgttttgaacgcttcatgtgtgaaccaccgtattgcatacaactgtaatattttgaacta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaccggaaaagtgaaaagaaacgctaatcaaaacatggcttgccatattgcaacaagcttactcaagctcaattttactgccatggagctgaaagataattagggcaattaaaggaaacggatttaccgattatacagactaaccaaggttgtctatcggcttgcccgttggcgtgagatgttaattcgcgatgaggttcactttatagcatacagcgcataagctgatgggaaaatttatctgcccaagttttgagaggcatgtgttaataaataatattttttgctccatagcattcatttctttccatggcatattgtttctccgagagattttgacactcattttacaagattgaaatataaacgatgttttgaacgcttcatgtgtgaaccaccgtattgcatacaactgtaatattttgaacta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02681:AA02682|AA02681:AA02682:hypothetical protein:hydrogenase maturation protein:->->:1866012..1866443 432 taaaccggaaaagtgaaaagaaacgctaatcaaaacatggcttgccatattgcaacaagcttactcaagctcaattttactgccatggagctgaaagataattagggcaattaaaggaaacggatttaccgattatacagactaaccaaggttgtctatcggcttgcccgttggcgtgagatgttaattcgcgatgaggttcactttatagcatacagcgcataagctgatgggaaaatttatctgcccaagttttgagaggcatgtgttaataaataatattttttgctccatagcattcatttctttccatggcatattgtttctccgagagattttgacactcattttacaagattgaaatataaacgatgttttgaacgcttcatgtgtgaaccaccgtattgcatacaactgtaatattttgaacta Intra-Species Hit: Count: 1 Min: 1 Max: 432 Len: 432 Subject: aact_AA02681_AA02682|hypothetical protein:hydrogenase maturation protein|POSITIVE:POSITIVE|[1866012,1866443]|432 HSP 1 e-value: 0.0 bit: 856.0 Len: 432 Query Start:1 Query End:432 Subject Strand: POSITIVE Subject Start: 1 Subject End: 432 taaaccggaaaagtgaaaagaaacgctaatcaaaacatggcttgccatattgcaacaagcttactcaagctcaattttactgccatggagctgaaagataattagggcaattaaaggaaacggatttaccgattatacagactaaccaaggttgtctatcggcttgcccgttggcgtgagatgttaattcgcgatgaggttcactttatagcatacagcgcataagctgatgggaaaatttatctgcccaagttttgagaggcatgtgttaataaataatattttttgctccatagcattcatttctttccatggcatattgtttctccgagagattttgacactcattttacaagattgaaatataaacgatgttttgaacgcttcatgtgtgaaccaccgtattgcatacaactgtaatattttgaacta taaaccggaaaagtgaaaagaaacgctaatcaaaacatggcttgccatattgcaacaagcttactcaagctcaattttactgccatggagctgaaagataattagggcaattaaaggaaacggatttaccgattatacagactaaccaaggttgtctatcggcttgcccgttggcgtgagatgttaattcgcgatgaggttcactttatagcatacagcgcataagctgatgggaaaatttatctgcccaagttttgagaggcatgtgttaataaataatattttttgctccatagcattcatttctttccatggcatattgtttctccgagagattttgacactcattttacaagattgaaatataaacgatgttttgaacgcttcatgtgtgaaccaccgtattgcatacaactgtaatattttgaacta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACCGGAAAAGUGAAAAGAAACGCUAAUCAAAACAUGGCUUGCCAUAUUGCAACAAGCUUACUCAAGCUCAAUUUUACUGCCAUGGAGCUGAAAGAUAAUUAGGGCAAUUAAAGGAAACGGAUUUACCGAUUAUACAGACUAACCAAGGUUGUCUAUCGGCUUGCCCGUUGGCGUGAGAUGUUAAUUCGCGAUGAGGUUCACUUUAUAGCAUACAGCGCAUAAGCUGAUGGGAAAAUUUAUCUGCCCAAGUUUUGAGAGGCAUGUGUUAAUAAAUAAUAUUUUUUGCUCCAUAGCAUUCAUUUCUUUCCAUGGCAUAUUGUUUCUCCGAGAGAUUUUGACACUCAUUUUACAAGAUUGAAAUAUAAACGAUGUUUUGAACGCUUCAUGUGUGAACCACCGUAUUGCAUACAACUGUAAUAUUUUGAACUA ......((...((((........))))..(((((((((.(((((.......((.....)).....)))))((((((((..((((((((((..((((((......(((((..((((.....(((.....)))........((((......)))).((((((((((((..(((((.(....)..........((.((((((....)))))).))...)))))..))))))))))))....))))..)))))..(((.((.(((.((.((((((.....))))))....))))))).)))..)).)))).))))))))))...((((((((...))))....))))..........))))))))..........)))))))))((((.....))))...))...((((((((......))))))))......... (-105.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUUCAAAAUAUUACAGUUGUAUGCAAUACGGUGGUUCACACAUGAAGCGUUCAAAACAUCGUUUAUAUUUCAAUCUUGUAAAAUGAGUGUCAAAAUCUCUCGGAGAAACAAUAUGCCAUGGAAAGAAAUGAAUGCUAUGGAGCAAAAAAUAUUAUUUAUUAACACAUGCCUCUCAAAACUUGGGCAGAUAAAUUUUCCCAUCAGCUUAUGCGCUGUAUGCUAUAAAGUGAACCUCAUCGCGAAUUAACAUCUCACGCCAACGGGCAAGCCGAUAGACAACCUUGGUUAGUCUGUAUAAUCGGUAAAUCCGUUUCCUUUAAUUGCCCUAAUUAUCUUUCAGCUCCAUGGCAGUAAAAUUGAGCUUGAGUAAGCUUGUUGCAAUAUGGCAAGCCAUGUUUUGAUUAGCGUUUCUUUUCACUUUUCCGGUUUA .................((((....)))).(((.(((........((((((((((((((((.((.............((((....((((.(.......).)))).....))))..(((((((............(((((((((((....((((.....)))).....................((((((...........(((((((......)))).)))...(((((..(((.......................(((....)))..(((((((((((((....))).)))).....))))))......)))..))))).)))))).............)))))))))))((((....((((((....)))))).))))..))))))).)).)))))))))...))))))).....)))...)))..... (-101.62) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 429 168 121 52 5 aact:AA01421|5end_phosphoglycolate_phosphatase_955055..955285_POSITIVE 381 200 151 115 57 aact:AA02224|5end_ABC_transport_system_permease_protein_1512282..1512512_POSITIVE 356 200 151 136 81 aact:AA00329|5end_molybdopterin-guanine_dinucleotide_biosynthesis_protein_222737..222967_POSITIVE 351 190 147 67 14 aact:AA02092|5end_glycogen_synthase_1424674..1424904_POSITIVE 350 196 151 95 41 aact:AA02697|5end_hydrogenase_3_maturation_protease_1879870..1880100_POSITIVE 347 207 164 177 135 aact:AA01062|5end_ribonuclease_PH_(RNase_PH)_735706..735936_POSITIVE 339 176 139 74 30 aact:AA00377|5end_acetyl-CoA_acetyltransferase_(Beta-ketothiolase,3-ketoacyl-CoA_thiolase)_253042..253272_POSITIVE 337 202 161 56 16 aact:AA00576|5end_conserved_hypothetical_protein_(possible_translation_factor)_394020..394250_POSITIVE 336 188 143 76 32 aact:AA01114|5end_alcohol_dehydrogenase,_class_III;_glutathione-dependent_formaldehyde_dehydrogenase_765307..765537_POSITIVE 333 268 227 191 148 aact:AA00282|5end_peptidyl-prolyl_cis-trans_isomerase;_macrophage_infectivity_potentiator_198834..199064_POSITIVE 332 230 190 211 167 aact:AA02047|5end_galactitol-1-phosphate_5-dehydrogenase_1393633..1393863_POSITIVE 332 183 149 46 1 aact:AA01418|5end_conserved_hypothetical_protein_953951..954181_POSITIVE 331 196 158 221 181 aact:AA01329|5end_LOS_biosynthesis_enzyme_(D-glycero-D-manno-heptosyl_transferase)_896630..896860_POSITIVE 330 181 146 153 115 aact:AA02926|5end_hypothetical_protein_2048136..2048366_POSITIVE 328 196 156 204 161 aact:AA00612|5end_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosami_ne_417840..418070_POSITIVE 326 206 161 72 17 aact:AA01852|5end_peptide_chain_release_factor_3_1244891..1245121_POSITIVE 325 178 137 163 109 aact:AA02538|5end_conserved_hypothetical_protein_1765187..1765417_POSITIVE 325 206 164 206 158 aact:AA01759|5end_hydrogenase_isoenzymes_formation_protein_1188675..1188905_POSITIVE 324 190 146 181 133 aact:AA00634|5end_DNA_polymerase_IV_(Pol_IV)_435182..435412_POSITIVE 323 200 151 218 160 aact:AA02845|5end_Holliday_junction_DNA_helicase_1997970..1998200_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 381 200 151 122 64 aact:AA02222|3end_conserved_hypothetical_protein_1512369..1512519_POSITIVE 350 196 151 102 48 aact:AA02696|3end_formate_hydrogenlyase_maturation_protein_1879957..1880107_POSITIVE 339 176 139 53 9 aact:AA00374|3end_short-chain_fatty_acids_transporter_253101..253251_POSITIVE 337 202 161 49 9 aact:AA00575|3end_shikimate_5-dehydrogenase_394093..394243_POSITIVE 332 194 153 57 7 aact:AA01417|3end_probable_4-diphosphocytidyl-2C-methyl-D-erthyritol_synthase_(MEP_cytidyltransferase)_(MCT)_954028..954178_POSITIVE 330 181 146 88 50 aact:AA02925|3end_conserved_hypothetical_protein_2048151..2048301_POSITIVE 324 190 146 86 38 aact:AA00633|3end_hypothetical_protein_435167..435317_POSITIVE 321 168 124 46 1 aact:AA01419|3end_D-ribulose-phosphate-3_epimerase_955129..955279_POSITIVE 321 250 210 140 94 aact:AA01113|3end_transcriptional_regulator_(merR_family)_765249..765399_POSITIVE 320 177 146 64 23 aact:AA01182|3end_3-oxoacyl-(acyl-carrier-protein)_synthase_810510..810660_POSITIVE 316 203 161 108 66 aact:AA02675|3end_formate_dehydrogenase_alpha_subunit_1861699..1861849_POSITIVE 311 189 142 129 88 aact:AA02199|3end_hypothetical_protein_1497901..1498051_POSITIVE 310 185 142 145 99 aact:AA02316|3end_exodeoxyribonuclease_VII_large_subunit_1583093..1583243_POSITIVE 308 199 151 82 35 aact:AA01486|3end_ATP_synthase_F1,_subunit_alpha_1000178..1000328_POSITIVE 308 93 59 132 93 aact:AA01476|3end_hypothetical_protein_992261..992411_POSITIVE 307 189 142 66 19 aact:AA01748|3end_thiol:disulfide_interchange_protein_1183810..1183960_POSITIVE 307 185 147 140 108 aact:AA01292|3end_single-stranded_DNA_binding_protein_876383..876533_POSITIVE 303 220 171 137 78 aact:AA01890|3end_cell-cycle_protein_1275967..1276117_POSITIVE 300 184 146 50 8 aact:AA00062|3end_bicyclomycin_resistance_protein_(Sulfonamide_resistance_protein)_48030..48180_POSITIVE 298 201 161 58 15 aact:AA02486|3end_topoisomerase_IV,_subunit_B_1726312..1726462_POSITIVE