Origin IGS: tgatcacaatcacaaaaaattatatttatgcgacatattcagcaatgaatggcattttttatcatataaatattgttaccgataacatttttacgttaagctaagctgattcagaatttcacgaaaaaggaaatcaca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgtgatttcctttttcgtgaaattctgaatcagcttagcttaacgtaaaaatgttatcggtaacaatatttatatgataaaaaatgccattcattgctgaatatgtcgcataaatataattttttgtgattgtgatca Mask Tandem Repeat Region ================================================ tgatcacaatcacaaaaaattatatttatgcgacatattcagcaatgaatggcattttttatcatataaatattgttaccgataacatttttacgttaagctaagctgattcagaatttcacgaaaaaggaaatcaca Find is-nt database================================================ Query_seq: AA02670:AA02671|AA02670:AA02671:hypothetical protein:gluconokinase:->->:1858324..1858461 138 tgatcacaatcacaaaaaattatatttatgcgacatattcagcaatgaatggcattttttatcatataaatattgttaccgataacatttttacgttaagctaagctgattcagaatttcacgaaaaaggaaatcaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02670:AA02671|AA02670:AA02671:hypothetical protein:gluconokinase:->->:1858324..1858461 138 tgatcacaatcacaaaaaattatatttatgcgacatattcagcaatgaatggcattttttatcatataaatattgttaccgataacatttttacgttaagctaagctgattcagaatttcacgaaaaaggaaatcaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02670:AA02671|AA02670:AA02671:hypothetical protein:gluconokinase:->->:1858324..1858461 138 tgatcacaatcacaaaaaattatatttatgcgacatattcagcaatgaatggcattttttatcatataaatattgttaccgataacatttttacgttaagctaagctgattcagaatttcacgaaaaaggaaatcaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgatcacaatcacaaaaaattatatttatgcgacatattcagcaatgaatggcattttttatcatataaatattgttaccgataacatttttacgttaagctaagctgattcagaatttcacgaaaaaggaaatcaca Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 3 End: 101 .. M T I T K N Y I Y A T Y S A M N G I F Y H I N I V T D N I F T L S ..................................... tgatcacaatcacaaaaaattatatttatgcgacatattcagcaatgaatggcattttttatcatataaatattgttaccgataacatttttacgttaagctaagctgattcagaatttcacgaaaaaggaaatcaca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgatcacaatcacaaaaaattatatttatgcgacatattcagcaatgaatggcattttttatcatataaatattgttaccgataacatttttacgttaagctaagctgattcagaatttcacgaaaaaggaaatcaca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02670:AA02671|AA02670:AA02671:hypothetical protein:gluconokinase:->->:1858324..1858461 138 tgatcacaatcacaaaaaattatatttatgcgacatattcagcaatgaatggcattttttatcatataaatattgttaccgataacatttttacgttaagctaagctgattcagaatttcacgaaaaaggaaatcaca Intra-Species Hit: Count: 1 Min: 1 Max: 138 Len: 138 Subject: aact_AA02670_AA02671|hypothetical protein:gluconokinase|POSITIVE:POSITIVE|[1858324, 1858461]|138 HSP 1 e-value: 7.0E-73 bit: 274.0 Len: 138 Query Start:1 Query End:138 Subject Strand: POSITIVE Subject Start: 1 Subject End: 138 tgatcacaatcacaaaaaattatatttatgcgacatattcagcaatgaatggcattttttatcatataaatattgttaccgataacatttttacgttaagctaagctgattcagaatttcacgaaaaaggaaatcaca tgatcacaatcacaaaaaattatatttatgcgacatattcagcaatgaatggcattttttatcatataaatattgttaccgataacatttttacgttaagctaagctgattcagaatttcacgaaaaaggaaatcaca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUCACAAUCACAAAAAAUUAUAUUUAUGCGACAUAUUCAGCAAUGAAUGGCAUUUUUUAUCAUAUAAAUAUUGUUACCGAUAACAUUUUUACGUUAAGCUAAGCUGAUUCAGAAUUUCACGAAAAAGGAAAUCACA .((((((..........(((.(((((((((.((..((((((....))))))..........)).))))))))).))).....((((........))))......).)))))..((.((((........)))).))... (-15.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUGAUUUCCUUUUUCGUGAAAUUCUGAAUCAGCUUAGCUUAACGUAAAAAUGUUAUCGGUAACAAUAUUUAUAUGAUAAAAAAUGCCAUUCAUUGCUGAAUAUGUCGCAUAAAUAUAAUUUUUUGUGAUUGUGAUCA ...(((((((......).)))))).....(((((......((((((....))))))..((((....((((.....)))).....)))).......)))))....((((((...(((........)))...)))))).. (-20.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 275 53 25 32 10 aact:AA02878|5end_conserved_hypothetical_protein_2018790..2019020_POSITIVE 237 63 28 209 173 aact:AA01120|5end_conserved_hypothetical_protein_769449..769679_POSITIVE 237 63 28 51 15 aact:AA01119|5end_hypothetical_protein_769291..769521_POSITIVE 235 110 73 216 178 aact:AA02179|5end_anthanilate_phosphoribosyltransferase_1489367..1489597_POSITIVE 225 97 51 69 30 aact:AA00106|5end_1-hydroxy-2-methyl-2-(E)-butenyl_4-diphosphate_synthase_75544..75774_POSITIVE 225 64 23 77 28 aact:AA00088|5end_30S_ribosomal_protein_S15_66041..66271_POSITIVE 217 109 66 122 87 aact:AA01151|5end_porphobilinogen_synthase_(delta-aminolevulinic_acid_dehydratase)_788323..788553_POSITIVE 214 112 71 41 1 aact:AA01973|5end_acetate_kinase_1334424..1334654_POSITIVE 210 54 28 35 9 aact:AA02124|5end_anaerobic_regulatory_protein;_transcription_regulator_Fnr_1448520..1448750_POSITIVE 209 116 76 156 127 aact:AA01441|5end_tRNA/rRNA_methylase_966490..966720_POSITIVE 208 114 78 207 165 aact:AA01418|5end_conserved_hypothetical_protein_953951..954181_POSITIVE 207 129 84 41 3 aact:AA02907|5end_thiol:disulfide_interchange_protein;_inner_membrane_copper_tolerance_protein_2033477..2033707_POSITIVE 205 130 82 193 134 aact:AA00538|5end_hypothetical_protein_368906..369136_POSITIVE 204 81 42 140 107 aact:AA02470|5end_glucose_kinase_1706730..1706960_POSITIVE 203 54 18 100 58 aact:AA02103|5end_D-alanyl-D-alanine-endopeptidase;_penicillin-binding_protein_7_1431105..1431335_POSITIVE 202 65 26 210 170 aact:AA00889|5end_conserved_hypothetical_protein_616857..617087_POSITIVE 202 65 26 92 52 aact:AA00887|5end_hypothetical_protein_616739..616969_POSITIVE 197 117 73 160 105 aact:AA02362|5end_conserved_hypothetical_protein_1619905..1620135_POSITIVE 195 52 22 201 165 aact:AA00943|5end_conserved_hypothetical_protein_654331..654561_POSITIVE 194 100 74 27 1 aact:AA02670|5end_hypothetical_protein_1858049..1858279_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 237 63 28 55 19 aact:AA01117|3end_signal_recognition_particle_protein_54_(GTP-binding_export_factor)_769375..769525_POSITIVE 225 97 51 54 15 aact:AA00105|3end_conserved_hypothetical_protein_75609..75759_POSITIVE 209 116 76 84 55 aact:AA01439|3end_probable_ferredoxin_(fdx-2)_966498..966648_POSITIVE 204 81 42 127 94 aact:AA02468|3end_conserved_hypothetical_protein_1706797..1706947_POSITIVE 203 54 18 103 61 aact:AA02101|3end_penicillin-binding_protein_7_1431188..1431338_POSITIVE 202 65 26 128 88 aact:AA00886|3end_hypothetical_protein_616855..617005_POSITIVE 197 117 73 122 67 aact:AA02360|3end_threonine_synthase_1619947..1620097_POSITIVE 194 69 24 88 38 aact:AA01832|3end_formate_dehydrogenase_formation_protein_E_1234603..1234753_POSITIVE 193 113 78 78 46 aact:AA02558|3end_conserved_hypothetical_protein_1781480..1781630_POSITIVE 190 48 26 41 10 aact:AA02546|3end_hypothetical_protein_1774761..1774911_POSITIVE 190 54 23 119 86 aact:AA01871|3end_conserved_hypothetical_protein_1258710..1258860_POSITIVE 190 62 30 117 76 aact:AA00849|3end_MraZ_cell_division_protein_586786..586936_POSITIVE 188 64 41 90 63 aact:AA00796|3end_iron(III)_dicitrate_transport_system_permease_protein_546752..546902_POSITIVE 185 129 86 62 7 aact:AA01274|3end_hypothetical_protein_864832..864982_POSITIVE 184 107 76 132 95 aact:AA00867|3end_fimbrial_protein_Flp_precursor_600991..601141_POSITIVE 184 120 78 109 59 aact:AA00512|3end_conserved_hypothetical_protein_354649..354799_POSITIVE 183 67 24 79 36 aact:AA01149|3end_glycyl-tRNA_synthetase_beta_subunit_788185..788335_POSITIVE 182 62 22 146 97 aact:AA00157|3end_hypothetical_protein_112324..112474_POSITIVE 181 64 37 57 34 aact:AA01190|3end_glycosyl_transferase_(possible_dolichol-phosphate_mannosyltransferase)_816077..816227_POSITIVE 181 65 25 111 74 aact:AA00253|3end_hypothetical_protein_177636..177786_POSITIVE