Origin IGS: tattcaatcaaattcaaacatcacatcatcaaacttattttagaagagcattgaggcattcgtgaagtcacacaacacgatgcccattcttctgttatagt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 actataacagaagaatgggcatcgtgttgtgtgacttcacgaatgcctcaatgctcttctaaaataagtttgatgatgtgatgtttgaatttgattgaata Mask Tandem Repeat Region ================================================ tattcaatcaaattcaaacatcacatcatcaaacttattttagaagagcattgaggcattcgtgaagtcacacaacacgatgcccattcttctgttatagt Find is-nt database================================================ Query_seq: AA02629:AA02630|AA02629:AA02630:hypothetical protein:hypothetical protein:->->:1830335..1830435 101 tattcaatcaaattcaaacatcacatcatcaaacttattttagaagagcattgaggcattcgtgaagtcacacaacacgatgcccattcttctgttatagt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02629:AA02630|AA02629:AA02630:hypothetical protein:hypothetical protein:->->:1830335..1830435 101 tattcaatcaaattcaaacatcacatcatcaaacttattttagaagagcattgaggcattcgtgaagtcacacaacacgatgcccattcttctgttatagt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02629:AA02630|AA02629:AA02630:hypothetical protein:hypothetical protein:->->:1830335..1830435 101 tattcaatcaaattcaaacatcacatcatcaaacttattttagaagagcattgaggcattcgtgaagtcacacaacacgatgcccattcttctgttatagt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tattcaatcaaattcaaacatcacatcatcaaacttattttagaagagcattgaggcattcgtgaagtcacacaacacgatgcccattcttctgttatagt Predict ORF larger than 30AA ================================================ tattcaatcaaattcaaacatcacatcatcaaacttattttagaagagcattgaggcattcgtgaagtcacacaacacgatgcccattcttctgttatagt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tattcaatcaaattcaaacatcacatcatcaaacttattttagaagagcattgaggcattcgtgaagtcacacaacacgatgcccattcttctgttatagt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02629:AA02630|AA02629:AA02630:hypothetical protein:hypothetical protein:->->:1830335..1830435 101 tattcaatcaaattcaaacatcacatcatcaaacttattttagaagagcattgaggcattcgtgaagtcacacaacacgatgcccattcttctgttatagt Intra-Species Hit: Count: 1 Min: 1 Max: 101 Len: 101 Subject: aact_AA02629_AA02630|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1830335,1830435]|101 HSP 1 e-value: 6.0E-51 bit: 200.0 Len: 101 Query Start:1 Query End:101 Subject Strand: POSITIVE Subject Start: 1 Subject End: 101 tattcaatcaaattcaaacatcacatcatcaaacttattttagaagagcattgaggcattcgtgaagtcacacaacacgatgcccattcttctgttatagt tattcaatcaaattcaaacatcacatcatcaaacttattttagaagagcattgaggcattcgtgaagtcacacaacacgatgcccattcttctgttatagt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUUCAAUCAAAUUCAAACAUCACAUCAUCAAACUUAUUUUAGAAGAGCAUUGAGGCAUUCGUGAAGUCACACAACACGAUGCCCAUUCUUCUGUUAUAGU ..................................((((..((((((((...((.(((((.((((..(.....)..)))))))))))))))))))..)))). (-22.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACUAUAACAGAAGAAUGGGCAUCGUGUUGUGUGACUUCACGAAUGCCUCAAUGCUCUUCUAAAAUAAGUUUGAUGAUGUGAUGUUUGAAUUUGAUUGAAUA ........((((((.(((((((((((..(.....)..)))).))))).))....))))))....((((((..(...........)..))))))........ (-21.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 300 100 51 59 4 aact:AA02675|5end_formate_dehydrogenase_alpha_subunit_1861011..1861241_POSITIVE 279 84 46 70 26 aact:AA02940|5end_conserved_hypothetical_protein_2057922..2058152_POSITIVE 278 94 54 136 86 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 275 92 55 83 38 aact:AA00596|5end_uridylate_kinase_407496..407726_POSITIVE 271 87 42 61 2 aact:AA02067|5end_conserved_hypothetical_protein;_possible_membrane_protein_1405233..1405463_POSITIVE 270 87 45 185 141 aact:AA02688|5end_hydrogenase_4_component_E_1873977..1874207_POSITIVE 263 30 1 208 179 aact:AA00644|5end_lipoprotein_440785..441015_POSITIVE 258 84 48 230 184 aact:AA00952|5end_hemY_protein_homolog_657541..657771_POSITIVE 253 85 48 221 179 aact:AA01684|5end_ABC_transporter,_ATP-binding_protein;_possible_ATP-binding_component_of_sulfate_permease_A_protein_1136302..1136532_POSITIVE 251 98 56 173 132 aact:AA01961|5end_lipid_A_export_ATP-binding/permease_protein_msbA_1324405..1324635_POSITIVE 251 80 34 64 21 aact:AA01289|5end_hypothetical_protein_872385..872615_POSITIVE 248 95 59 98 54 aact:AA01019|5end_conserved_hypothetical_protein_712223..712453_POSITIVE 248 88 55 218 182 aact:AA00528|5end_conserved_hypothetical_protein_361396..361626_POSITIVE 242 85 48 107 67 aact:AA01556|5end_ABC_transporter_ATP-binding_protein_1044218..1044448_POSITIVE 238 95 56 47 2 aact:AA00372|5end_acetate_CoA-transferase_(alpha_subunit)_250348..250578_POSITIVE 236 88 48 214 174 aact:AA00817|5end_MutT-family_protein_561775..562005_POSITIVE 235 88 41 174 119 aact:AA01939|5end_NAD(P)_transhydrogenase_subunit_beta_1311039..1311269_POSITIVE 235 97 52 191 149 aact:AA00753|5end_galactoside_ABC_transporter,_permease_protein_517528..517758_POSITIVE 233 84 49 57 13 aact:AA01429|5end_conserved_hypothetical_protein_959581..959811_POSITIVE 232 90 44 199 141 aact:AA00824|5end_hypothetical_protein_566920..567150_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 334 49 1 151 103 aact:AA01549|3end_hypothetical_protein_1037880..1038030_POSITIVE 271 87 42 62 3 aact:AA02066|3end_hypothetical_protein_1405314..1405464_POSITIVE 263 30 1 37 8 aact:AA00643|3end_hypothetical_protein_440694..440844_POSITIVE 253 83 42 68 23 aact:AA02340|3end_hypothetical_protein_1599341..1599491_POSITIVE 253 83 42 76 31 aact:AA02115|3end_hypothetical_protein_1444991..1445141_POSITIVE 253 83 42 76 31 aact:AA01075|3end_hypothetical_protein_746068..746218_POSITIVE 253 92 54 126 81 aact:AA00884|3end_sodium-dependent_transporter_615971..616121_POSITIVE 253 83 42 76 31 aact:AA00008|3end_hypothetical_protein_(possible_transposase_fragment)_5495..5645_POSITIVE 251 98 56 151 110 aact:AA01959|3end_tetraacyldisaccharide_4'-kinase_1324463..1324613_POSITIVE 249 93 52 49 5 aact:AA02629|3end_hypothetical_protein_1830274..1830424_POSITIVE 248 95 59 95 51 aact:AA01018|3end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_712300..712450_POSITIVE 242 85 48 132 92 aact:AA01555|3end_ABC_transporter,_ATP-binding_protein_1044323..1044473_POSITIVE 239 97 56 149 109 aact:AA00249|3end_cysteinyl-tRNA_synthetase_(cysteine--tRNA_ligase)_175422..175572_POSITIVE 236 88 48 124 84 aact:AA00816|3end_lacZ_expression_regulator_561765..561915_POSITIVE 231 95 56 136 97 aact:AA01431|3end_conserved_hypothetical_protein_(possible_hydrolase)_960751..960901_POSITIVE 231 87 46 56 13 aact:AA00278|3end_conserved_hypothetical_protein_195358..195508_POSITIVE 229 86 55 117 84 aact:AA01273|3end_hypothetical_protein_864588..864738_POSITIVE 228 88 43 118 77 aact:AA02495|3end_conserved_hypothetical_protein_1731992..1732142_POSITIVE 228 98 54 137 97 aact:AA00482|3end_hydrolase_(haloacid_dehalogenase-like)_(HAD_superfamily)_336484..336634_POSITIVE 226 93 51 71 29 aact:AA00461|3end_heat_shock_protein;_protease_322979..323129_POSITIVE