Origin IGS: taacacaaaaattcaccgcacttataggcttttaggcacagtaccgaaaccagaacaagggctgtgctttctgacttcattttacactgctatacaacctctttcagccggccggcacgatagtaagtttattttacttatactattcatcttaacaaatccacactaacaatagggtaatttttacacaaacagttctttttaaacttgaatttaagcgaattttaaataaaatgcttctcatttgattttaacaacaatagatacaaattaagatattt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaatatcttaatttgtatctattgttgttaaaatcaaatgagaagcattttatttaaaattcgcttaaattcaagtttaaaaagaactgtttgtgtaaaaattaccctattgttagtgtggatttgttaagatgaatagtataagtaaaataaacttactatcgtgccggccggctgaaagaggttgtatagcagtgtaaaatgaagtcagaaagcacagcccttgttctggtttcggtactgtgcctaaaagcctataagtgcggtgaatttttgtgtta Mask Tandem Repeat Region ================================================ taacacaaaaattcaccgcacttataggcttttaggcacagtaccgaaaccagaacaagggctgtgctttctgacttcattttacactgctatacaacctctttcagccggccggcacgatagtaagtttattttacttatactattcatcttaacaaatccacactaacaatagggtaatttttacacaaacagttctttttaaacttgaatttaagcgaattttaaataaaatgcttctcatttgattttaacaacaatagatacaaattaagatattt Find is-nt database================================================ Query_seq: AA02619:AA02620|AA02619:AA02620:GTP cyclohydrolase II:orf14; possible membrane protein:->->:1826254..1826534 281 taacacaaaaattcaccgcacttataggcttttaggcacagtaccgaaaccagaacaagggctgtgctttctgacttcattttacactgctatacaacctctttcagccggccggcacgatagtaagtttattttacttatactattcatcttaacaaatccacactaacaatagggtaatttttacacaaacagttctttttaaacttgaatttaagcgaattttaaataaaatgcttctcatttgattttaacaacaatagatacaaattaagatattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02619:AA02620|AA02619:AA02620:GTP cyclohydrolase II:orf14; possible membrane protein:->->:1826254..1826534 281 taacacaaaaattcaccgcacttataggcttttaggcacagtaccgaaaccagaacaagggctgtgctttctgacttcattttacactgctatacaacctctttcagccggccggcacgatagtaagtttattttacttatactattcatcttaacaaatccacactaacaatagggtaatttttacacaaacagttctttttaaacttgaatttaagcgaattttaaataaaatgcttctcatttgattttaacaacaatagatacaaattaagatattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02619:AA02620|AA02619:AA02620:GTP cyclohydrolase II:orf14; possible membrane protein:->->:1826254..1826534 281 taacacaaaaattcaccgcacttataggcttttaggcacagtaccgaaaccagaacaagggctgtgctttctgacttcattttacactgctatacaacctctttcagccggccggcacgatagtaagtttattttacttatactattcatcttaacaaatccacactaacaatagggtaatttttacacaaacagttctttttaaacttgaatttaagcgaattttaaataaaatgcttctcatttgattttaacaacaatagatacaaattaagatattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacacaaaaattcaccgcacttataggcttttaggcacagtaccgaaaccagaacaagggctgtgctttctgacttcattttacactgctatacaacctctttcagccggccggcacgatagtaagtttattttacttatactattcatcttaacaaatccacactaacaatagggtaatttttacacaaacagttctttttaaacttgaatttaagcgaattttaaataaaatgcttctcatttgattttaacaacaatagatacaaattaagatattt Predict ORF larger than 30AA ================================================ Protein_Len: 52 Strand: + Start: 11 End: 166 .......... M H R T Y R L L G T V P K P E Q G L C F L T S F Y T A I Q P L S A G R H D S K F I L L I L F I L T N P H ................................................................................................................... Protein_Len: 30 Strand: - Start: 155 End: 244 .......................................................................................................................................................... C I W V L L L T I K V C V T R K L S S N L R I K F L I S R M ..................................... taacacaaaaattcaccgcacttataggcttttaggcacagtaccgaaaccagaacaagggctgtgctttctgacttcattttacactgctatacaacctctttcagccggccggcacgatagtaagtttattttacttatactattcatcttaacaaatccacactaacaatagggtaatttttacacaaacagttctttttaaacttgaatttaagcgaattttaaataaaatgcttctcatttgattttaacaacaatagatacaaattaagatattt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacacaaaaattcaccgcacttataggcttttaggcacagtaccgaaaccagaacaagggctgtgctttctgacttcattttacactgctatacaacctctttcagccggccggcacgatagtaagtttattttacttatactattcatcttaacaaatccacactaacaatagggtaatttttacacaaacagttctttttaaacttgaatttaagcgaattttaaataaaatgcttctcatttgattttaacaacaatagatacaaattaagatattt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02619:AA02620|AA02619:AA02620:GTP cyclohydrolase II:orf14; possible membrane protein:->->:1826254..1826534 281 taacacaaaaattcaccgcacttataggcttttaggcacagtaccgaaaccagaacaagggctgtgctttctgacttcattttacactgctatacaacctctttcagccggccggcacgatagtaagtttattttacttatactattcatcttaacaaatccacactaacaatagggtaatttttacacaaacagttctttttaaacttgaatttaagcgaattttaaataaaatgcttctcatttgattttaacaacaatagatacaaattaagatattt Intra-Species Hit: Count: 1 Min: 1 Max: 281 Len: 281 Subject: aact_AA02619_AA02620|GTP cyclohydrolase II:orf14; possible membrane protein|POSITIVE:POSITIVE|[1826254,1826534]|281 HSP 1 e-value: 1.0E-158 bit: 557.0 Len: 281 Query Start:1 Query End:281 Subject Strand: POSITIVE Subject Start: 1 Subject End: 281 taacacaaaaattcaccgcacttataggcttttaggcacagtaccgaaaccagaacaagggctgtgctttctgacttcattttacactgctatacaacctctttcagccggccggcacgatagtaagtttattttacttatactattcatcttaacaaatccacactaacaatagggtaatttttacacaaacagttctttttaaacttgaatttaagcgaattttaaataaaatgcttctcatttgattttaacaacaatagatacaaattaagatattt taacacaaaaattcaccgcacttataggcttttaggcacagtaccgaaaccagaacaagggctgtgctttctgacttcattttacactgctatacaacctctttcagccggccggcacgatagtaagtttattttacttatactattcatcttaacaaatccacactaacaatagggtaatttttacacaaacagttctttttaaacttgaatttaagcgaattttaaataaaatgcttctcatttgattttaacaacaatagatacaaattaagatattt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACACAAAAAUUCACCGCACUUAUAGGCUUUUAGGCACAGUACCGAAACCAGAACAAGGGCUGUGCUUUCUGACUUCAUUUUACACUGCUAUACAACCUCUUUCAGCCGGCCGGCACGAUAGUAAGUUUAUUUUACUUAUACUAUUCAUCUUAACAAAUCCACACUAACAAUAGGGUAAUUUUUACACAAACAGUUCUUUUUAAACUUGAAUUUAAGCGAAUUUUAAAUAAAAUGCUUCUCAUUUGAUUUUAACAACAAUAGAUACAAAUUAAGAUAUUU .................((.......((((...(((((((((.((.............)))))))))))..((....))..........................)))).....))..((((((((((.......)))..))))))).(((((((...((((............))))................(((....(((((.(..(((..((((..(((((....)))))))))...)))..).))))).)))............))))))).... (-36.62) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUAUCUUAAUUUGUAUCUAUUGUUGUUAAAAUCAAAUGAGAAGCAUUUUAUUUAAAAUUCGCUUAAAUUCAAGUUUAAAAAGAACUGUUUGUGUAAAAAUUACCCUAUUGUUAGUGUGGAUUUGUUAAGAUGAAUAGUAUAAGUAAAAUAAACUUACUAUCGUGCCGGCCGGCUGAAAGAGGUUGUAUAGCAGUGUAAAAUGAAGUCAGAAAGCACAGCCCUUGUUCUGGUUUCGGUACUGUGCCUAAAAGCCUAUAAGUGCGGUGAAUUUUUGUGUUA ........(((..(((.(((....(((.......)))...))).)))..))).........(((..(((((((............((((((..(.(..(((((((.(((....))).)).)))))....).)..))))))...(((((.......)))))..((..(.....((((.....((((......((((((.....((((..((((..(((.......)))))))..)))).))))))))))...)))).....)..)).)))))))..)))... (-54.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 366 119 80 195 131 aact:AA02487|5end_hypothetical_protein_1726277..1726507_POSITIVE 352 119 75 212 159 aact:AA02025|5end_hypothetical_protein_1378667..1378897_POSITIVE 337 121 85 184 137 aact:AA01957|5end_conserved_hypothetical_protein_1323211..1323441_POSITIVE 328 120 72 150 95 aact:AA01418|5end_conserved_hypothetical_protein_953951..954181_POSITIVE 326 41 2 154 113 aact:AA01441|5end_tRNA/rRNA_methylase_966490..966720_POSITIVE 312 122 86 57 15 aact:AA01755|5end_cobalt_membrane_transport_protein_1185825..1186055_POSITIVE 310 120 71 72 29 aact:AA02756|5end_phosphoserine_aminotransferase_1929477..1929707_POSITIVE 310 42 7 225 190 aact:AA01621|5end_replicative_DNA_helicase_1095647..1095877_POSITIVE 310 42 7 91 56 aact:AA01620|5end_hypothetical_protein_1095513..1095743_POSITIVE 307 132 103 176 139 aact:AA00811|5end_conserved_hypothetical_protein_556736..556966_POSITIVE 301 47 8 67 28 aact:AA01770|5end_hypothetical_protein_1193111..1193341_POSITIVE 301 117 71 192 134 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 299 68 26 50 4 aact:AA02573|5end_ABC_transporter,_ATP-binding_protein_1790409..1790639_POSITIVE 299 60 11 211 163 aact:AA00820|5end_succinyl-diaminopimelate_desuccinylase_563326..563556_POSITIVE 299 117 75 207 155 aact:AA00416|5end_D-methionine_transport_system_permease_protein_(ABC_transport_system)_282677..282907_POSITIVE 296 123 85 48 1 aact:AA01875|5end_PTS_system,_glucose-specific_enzyme_II,_A_component_1262563..1262793_POSITIVE 295 120 84 70 27 aact:AA01517|5end_peptidyl-prolyl_cis-trans_isomerase;_macrophage_infectivity_protein_1020989..1021219_POSITIVE 291 66 26 85 39 aact:AA01039|5end_transcriptional_regulator_(possible_murein_gene_regulator)_723864..724094_POSITIVE 289 57 12 145 108 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 285 48 15 155 113 aact:AA01537|5end_possible_glucokinase_regulatory_protein_1030616..1030846_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 366 119 80 150 86 aact:AA02486|3end_topoisomerase_IV,_subunit_B_1726312..1726462_POSITIVE 328 120 72 147 92 aact:AA01417|3end_probable_4-diphosphocytidyl-2C-methyl-D-erthyritol_synthase_(MEP_cytidyltransferase)_(MCT)_954028..954178_POSITIVE 326 41 2 82 41 aact:AA01439|3end_probable_ferredoxin_(fdx-2)_966498..966648_POSITIVE 312 122 86 54 12 aact:AA01753|3end_conserved_hypothetical_protein_1185902..1186052_POSITIVE 310 42 7 76 41 aact:AA01619|3end_phospho-2-dehydro-3-deoxyheptonate_aldolase_(DAHP_synthase)_1095578..1095728_POSITIVE 308 119 75 143 97 aact:AA02465|3end_glucosamine-6-phosphate_isomerase_1704885..1705035_POSITIVE 307 132 103 91 54 aact:AA00810|3end_alcohol_dehydrogenase_556731..556881_POSITIVE 292 36 2 95 56 aact:AA02865|3end_iron-regulated_outer_membrane_protein_(omp64)_2012542..2012692_POSITIVE 291 66 26 53 7 aact:AA01037|3end_hypothetical_protein_723912..724062_POSITIVE 291 123 85 149 112 aact:AA00335|3end_PTS_fructose-specific_IIBC_component_228230..228380_POSITIVE 289 57 12 84 47 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 281 120 77 85 47 aact:AA01861|3end_phosphomethylpyrimidine_kinase_1252107..1252257_POSITIVE 279 48 2 55 4 aact:AA01530|3end_conserved_hypothetical_protein_1028749..1028899_POSITIVE 279 90 45 53 13 aact:AA00164|3end_dephospho-CoA_kinase_(dephosphocoenzyme_A_kinase)_117445..117595_POSITIVE 277 122 87 137 81 aact:AA01948|3end_ABC_transporter_permease,_membrane_spanning_protein_1318196..1318346_POSITIVE 276 124 81 49 3 aact:AA01537|3end_possible_glucokinase_regulatory_protein_1031607..1031757_POSITIVE 275 120 80 143 100 aact:AA00345|3end_transcription_antitermination_protein_232058..232208_POSITIVE 274 46 8 98 69 aact:AA02697|3end_hydrogenase_3_maturation_protease_1880408..1880558_POSITIVE 274 60 14 49 2 aact:AA01842|3end_conserved_hypothetical_protein_1238467..1238617_POSITIVE 273 34 2 98 63 aact:AA00579|3end_probable_DNA_recombination_protein_396955..397105_POSITIVE