Origin IGS: taaaaggaagaacaataaaaacgaggaaaaaaattaccgcactttccgatgcaactcacacagcatgaattaaaccggatttatgcagcgggttaacgccttatattaagattgaaaaaatgcctgataatgaaatttatcaggcattttttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaaaaatgcctgataaatttcattatcaggcattttttcaatcttaatataaggcgttaacccgctgcataaatccggtttaattcatgctgtgtgagttgcatcggaaagtgcggtaatttttttcctcgtttttattgttcttcctttta Mask Tandem Repeat Region ================================================ taaaaggaagaacaataaaaacgaggaaaaaaattaccgcactttccgatgcaactcacacagcatgaattaaaccggatttatgcagcgggttaacgccttatattaagattgaaaaaatgcctgataatgaaatttatcaggcattttttta Find is-nt database================================================ Query_seq: AA02610:AA02611|AA02610:AA02611:acyl-CoA thioesterase II:hypothetical protein:->->:1821375..1821528 154 taaaaggaagaacaataaaaacgaggaaaaaaattaccgcactttccgatgcaactcacacagcatgaattaaaccggatttatgcagcgggttaacgccttatattaagattgaaaaaatgcctgataatgaaatttatcaggcattttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02610:AA02611|AA02610:AA02611:acyl-CoA thioesterase II:hypothetical protein:->->:1821375..1821528 154 taaaaggaagaacaataaaaacgaggaaaaaaattaccgcactttccgatgcaactcacacagcatgaattaaaccggatttatgcagcgggttaacgccttatattaagattgaaaaaatgcctgataatgaaatttatcaggcattttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02610:AA02611|AA02610:AA02611:acyl-CoA thioesterase II:hypothetical protein:->->:1821375..1821528 154 taaaaggaagaacaataaaaacgaggaaaaaaattaccgcactttccgatgcaactcacacagcatgaattaaaccggatttatgcagcgggttaacgccttatattaagattgaaaaaatgcctgataatgaaatttatcaggcattttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaaggaagaacaataaaaacgaggaaaaaaattaccgcactttccgatgcaactcacacagcatgaattaaaccggatttatgcagcgggttaacgccttatattaagattgaaaaaatgcctgataatgaaatttatcaggcattttttta Predict ORF larger than 30AA ================================================ taaaaggaagaacaataaaaacgaggaaaaaaattaccgcactttccgatgcaactcacacagcatgaattaaaccggatttatgcagcgggttaacgccttatattaagattgaaaaaatgcctgataatgaaatttatcaggcattttttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaaggaagaacaataaaaacgaggaaaaaaattaccgcactttccgatgcaactcacacagcatgaattaaaccggatttatgcagcgggttaacgccttatattaagattgaaaaaatgcctgataatgaaatttatcaggcattttttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02610:AA02611|AA02610:AA02611:acyl-CoA thioesterase II:hypothetical protein:->->:1821375..1821528 154 taaaaggaagaacaataaaaacgaggaaaaaaattaccgcactttccgatgcaactcacacagcatgaattaaaccggatttatgcagcgggttaacgccttatattaagattgaaaaaatgcctgataatgaaatttatcaggcattttttta Intra-Species Hit: Count: 1 Min: 1 Max: 146 Len: 146 Subject: aact_AA02610_AA02611|acyl-CoA thioesterase II:hypothetical protein|POSITIVE:POSITIVE|[1821375,1821528]|154 HSP 1 e-value: 5.0E-65 bit: 248.0 Len: 146 Query Start:1 Query End:146 Subject Strand: POSITIVE Subject Start: 1 Subject End: 146 taaaaggaagaacaataaaaacgaggnnnnnnnttaccgcactttccgatgcaactcacacagcatgaattaaaccggatttatgcagcgggttaacgccttatattaagattgaaaaaatgcctgataatgaaatttatcaggca........ taaaaggaagaacaataaaaacgaggaaaaaaattaccgcactttccgatgcaactcacacagcatgaattaaaccggatttatgcagcgggttaacgccttatattaagattgaaaaaatgcctgataatgaaatttatcaggca........ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAGGAAGAACAAUAAAAACGAGGAAAAAAAUUACCGCACUUUCCGAUGCAACUCACACAGCAUGAAUUAAACCGGAUUUAUGCAGCGGGUUAACGCCUUAUAUUAAGAUUGAAAAAAUGCCUGAUAAUGAAAUUUAUCAGGCA ............((((......((((..........((((.......((......)).....(((((((((......))))))))).)))).......))))........))))......((((((((((......)))))))))) (-34.87) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGCCUGAUAAAUUUCAUUAUCAGGCAUUUUUUCAAUCUUAAUAUAAGGCGUUAACCCGCUGCAUAAAUCCGGUUUAAUUCAUGCUGUGUGAGUUGCAUCGGAAAGUGCGGUAAUUUUUUUCCUCGUUUUUAUUGUUCUUCCUUUUA ((((((((((......))))))))))............(((((.((((((.......(((((((...((((((.((((((((.....)))))))).))))))..)))))))............)))))))))))............ (-40.01) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 368 57 17 176 137 aact:AA00250|5end_lipoate_biosynthesis_protein_A_175496..175726_POSITIVE 363 59 14 173 119 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 350 64 21 67 26 aact:AA02846|5end_crossover_junction_endodeoxyribonuclease_1998648..1998878_POSITIVE 349 52 17 135 94 aact:AA01187|5end_conserved_hypothetical_protein_813331..813561_POSITIVE 345 65 21 153 115 aact:AA00409|5end_methionyl-tRNA_formyltransferase_273611..273841_POSITIVE 326 70 21 231 175 aact:AA01633|5end_probable_ferredoxin-like_protein_1104008..1104238_POSITIVE 321 100 56 181 143 aact:AA02455|5end_conserved_hypothetical_protein_1694353..1694583_POSITIVE 306 58 12 207 158 aact:AA00213|5end_cold_shock_protein_(negative_regulator_of_cspA_transcription)_147071..147301_POSITIVE 303 55 16 136 96 aact:AA01692|5end_hexulose-6-phosphate_synthase_1149178..1149408_POSITIVE 296 88 44 81 31 aact:AA02270|5end_endopeptidase_La;_lon_protease;_protease_La_homolog;_ATP-dependent_protease_LA_(lon-1)_1546870..1547100_POSITIVE 295 99 51 86 44 aact:AA02080|5end_ABC_transporter,_ATP-binding/permease_1412744..1412974_POSITIVE 295 102 65 225 187 aact:AA00470|5end_conserved_hypothetical_protein_(possible_Zn-dependent_protease_with_chaperone_function)_326809..327039_POSITIVE 294 53 18 121 83 aact:AA01728|5end_conserved_hypothetical_protein(related_to_HipA_protein)_1168337..1168567_POSITIVE 293 48 13 147 115 aact:AA02066|5end_hypothetical_protein_1405115..1405345_POSITIVE 293 49 6 70 25 aact:AA00196|5end_conserved_hypothetical_protein_137378..137608_POSITIVE 290 100 52 68 14 aact:AA00069|5end_conserved_hypothetical_protein_52465..52695_POSITIVE 289 90 45 209 164 aact:AA00440|5end_hypothetical_protein_305400..305630_POSITIVE 286 102 62 48 5 aact:AA02099|5end_50S_ribosomal_protein_L25_1429639..1429869_POSITIVE 285 79 36 174 113 aact:AA01978|5end_hypothetical_protein_1337055..1337285_POSITIVE 283 52 16 148 105 aact:AA02963|5end_opacity_associated_protein_B_2072302..2072532_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 349 52 17 125 84 aact:AA01185|3end_conserved_hypothetical_protein_(possible_membrane_protein)_813401..813551_POSITIVE 345 65 21 150 112 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 326 70 21 58 2 aact:AA01631|3end_dihydrodipicolinate_reductase_1103915..1104065_POSITIVE 306 58 12 103 54 aact:AA00212|3end_ribokinase_147047..147197_POSITIVE 295 99 51 55 13 aact:AA02078|3end_hypothetical_protein_1412793..1412943_POSITIVE 295 102 65 146 108 aact:AA00469|3end_inorganic_pyrophosphatase,_chain_A_326810..326960_POSITIVE 294 53 18 124 86 aact:AA01727|3end_conserved_hypothetical_protein_(possible_HipA_protein)_1168420..1168570_POSITIVE 293 78 35 125 79 aact:AA00486|3end_nucleoside_transporter_338026..338176_POSITIVE 293 49 6 54 9 aact:AA00195|3end_conserved_hypothetical_protein_137442..137592_POSITIVE 287 78 36 123 80 aact:AA00574|3end_regulatory_protein_392381..392531_POSITIVE 284 45 5 88 50 aact:AA02020|3end_conserved_hypothetical_protein_(possible_tetrapyrrole_methylase_family_protein)_1374742..1374892_POSITIVE 283 52 16 51 8 aact:AA02962|3end_recombination_associated_protein_2072285..2072435_POSITIVE 279 57 17 99 60 aact:AA00081|3end_fatty_acid_metabolism_regulator_protein_60767..60917_POSITIVE 278 53 17 61 26 aact:AA02964|3end_opacity_associated_protein_A_2074128..2074278_POSITIVE 277 100 51 150 98 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 275 98 52 146 94 aact:AA01750|3end_MTA/SAH_nucleosidase_[Includes:_5'-methylthioadenosine_nucleosidase;_S-adenosylhomocysteine_nucleosidase]_1184519..1184669_POSITIVE 275 45 10 123 86 aact:AA01552|3end_hypothetical_protein_1039300..1039450_POSITIVE 274 57 17 98 56 aact:AA02940|3end_conserved_hypothetical_protein_2058652..2058802_POSITIVE 274 52 13 64 21 aact:AA00345|3end_transcription_antitermination_protein_232058..232208_POSITIVE 272 49 18 146 112 aact:AA01735|3end_hypothetical_protein_1172714..1172864_POSITIVE