Origin IGS: ctaacgatcctttcttaaaatttaagtattaccagcaattagcctatacattaattaatctgaaaattaaaataatatactgatctacttaagaattagttagttgcctatgatttaggtcaaattgattttacatctttcagcaataacagataactatataaattaagtatagaatgaaaaaccgacttgtaactaggagatcttt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaagatctcctagttacaagtcggtttttcattctatacttaatttatatagttatctgttattgctgaaagatgtaaaatcaatttgacctaaatcataggcaactaactaattcttaagtagatcagtatattattttaattttcagattaattaatgtataggctaattgctggtaatacttaaattttaagaaaggatcgttag Mask Tandem Repeat Region ================================================ ctaacgatcctttcttaaaatttaagtattaccagcaattagcctatacattaattaatctgaaaattaaaataatatactgatctacttaagaattagttagttgcctatgatttaggtcaaattgattttacatctttcagcaataacagataactatataaattaagtatagaatgaaaaaccgacttgtaactaggagatcttt Find is-nt database================================================ Query_seq: AA02592:AA02593|AA02592:AA02593:conserved hypothetical protein:conserved hypothetical protein (exported protein):->->:1803990..1804197 208 ctaacgatcctttcttaaaatttaagtattaccagcaattagcctatacattaattaatctgaaaattaaaataatatactgatctacttaagaattagttagttgcctatgatttaggtcaaattgattttacatctttcagcaataacagataactatataaattaagtatagaatgaaaaaccgacttgtaactaggagatcttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02592:AA02593|AA02592:AA02593:conserved hypothetical protein:conserved hypothetical protein (exported protein):->->:1803990..1804197 208 ctaacgatcctttcttaaaatttaagtattaccagcaattagcctatacattaattaatctgaaaattaaaataatatactgatctacttaagaattagttagttgcctatgatttaggtcaaattgattttacatctttcagcaataacagataactatataaattaagtatagaatgaaaaaccgacttgtaactaggagatcttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02592:AA02593|AA02592:AA02593:conserved hypothetical protein:conserved hypothetical protein (exported protein):->->:1803990..1804197 208 ctaacgatcctttcttaaaatttaagtattaccagcaattagcctatacattaattaatctgaaaattaaaataatatactgatctacttaagaattagttagttgcctatgatttaggtcaaattgattttacatctttcagcaataacagataactatataaattaagtatagaatgaaaaaccgacttgtaactaggagatcttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctaacgatcctttcttaaaatttaagtattaccagcaattagcctatacattaattaatctgaaaattaaaataatatactgatctacttaagaattagttagttgcctatgatttaggtcaaattgattttacatctttcagcaataacagataactatataaattaagtatagaatgaaaaaccgacttgtaactaggagatcttt Predict ORF larger than 30AA ================================================ ctaacgatcctttcttaaaatttaagtattaccagcaattagcctatacattaattaatctgaaaattaaaataatatactgatctacttaagaattagttagttgcctatgatttaggtcaaattgattttacatctttcagcaataacagataactatataaattaagtatagaatgaaaaaccgacttgtaactaggagatcttt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctaacgatcctttcttaaaatttaagtattaccagcaattagcctatacattaattaatctgaaaattaaaataatatactgatctacttaagaattagttagttgcctatgatttaggtcaaattgattttacatctttcagcaataacagataactatataaattaagtatagaatgaaaaaccgacttgtaactaggagatcttt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02592:AA02593|AA02592:AA02593:conserved hypothetical protein:conserved hypothetical protein (exported protein):->->:1803990..1804197 208 ctaacgatcctttcttaaaatttaagtattaccagcaattagcctatacattaattaatctgaaaattaaaataatatactgatctacttaagaattagttagttgcctatgatttaggtcaaattgattttacatctttcagcaataacagataactatataaattaagtatagaatgaaaaaccgacttgtaactaggagatcttt Intra-Species Hit: Count: 1 Min: 1 Max: 208 Len: 208 Subject: aact_AA02592_AA02593|conserved hypothetical protein:conserved hypothetical protein (exported protein)|POSITIVE:POSITIVE|[1803990,1804197]|208 HSP 1 e-value: 1.0E-114 bit: 412.0 Len: 208 Query Start:1 Query End:208 Subject Strand: POSITIVE Subject Start: 1 Subject End: 208 ctaacgatcctttcttaaaatttaagtattaccagcaattagcctatacattaattaatctgaaaattaaaataatatactgatctacttaagaattagttagttgcctatgatttaggtcaaattgattttacatctttcagcaataacagataactatataaattaagtatagaatgaaaaaccgacttgtaactaggagatcttt ctaacgatcctttcttaaaatttaagtattaccagcaattagcctatacattaattaatctgaaaattaaaataatatactgatctacttaagaattagttagttgcctatgatttaggtcaaattgattttacatctttcagcaataacagataactatataaattaagtatagaatgaaaaaccgacttgtaactaggagatcttt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUAACGAUCCUUUCUUAAAAUUUAAGUAUUACCAGCAAUUAGCCUAUACAUUAAUUAAUCUGAAAAUUAAAAUAAUAUACUGAUCUACUUAAGAAUUAGUUAGUUGCCUAUGAUUUAGGUCAAAUUGAUUUUACAUCUUUCAGCAAUAACAGAUAACUAUAUAAAUUAAGUAUAGAAUGAAAAACCGACUUGUAACUAGGAGAUCUUU .....((((...(((((.....(((((.......((.....))((((((.((((((.(((((................((((((((.....)).))))))..(((((....(((..(((((.....)))))...))).....)))))..))))).........))))))))))))............)))))....)))))))))... (-29.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAGAUCUCCUAGUUACAAGUCGGUUUUUCAUUCUAUACUUAAUUUAUAUAGUUAUCUGUUAUUGCUGAAAGAUGUAAAAUCAAUUUGACCUAAAUCAUAGGCAACUAACUAAUUCUUAAGUAGAUCAGUAUAUUAUUUUAAUUUUCAGAUUAAUUAAUGUAUAGGCUAAUUGCUGGUAAUACUUAAAUUUUAAGAAAGGAUCGUUAG ...(((((.(((((....((((..............(((((((...((.((((((.......((((........)))).......(((.((((.....))))))).))))))))..))))))).....((((((((..((((((....)))))).)))))))).))))....))))).....((((.....))))...)))))..... (-34.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 208 145 101 192 140 aact:AA01859|5end_hypothetical_protein_1251718..1251948_POSITIVE 201 138 102 221 183 aact:AA01718|5end_DNA_polymerase_III,_chi_subunit_1162790..1163020_POSITIVE 198 110 77 105 66 aact:AA02609|5end_ABC_transporter,_ATP-binding_protein/permease_1818446..1818676_POSITIVE 197 64 28 76 44 aact:AA01079|5end_conserved_hypothetical_protein_747404..747634_POSITIVE 197 196 163 185 153 aact:AA00552|5end_periplasmic_nitrate_reductase_precursor_377331..377561_POSITIVE 197 196 163 79 47 aact:AA00551|5end_hypothetical_protein_377225..377455_POSITIVE 195 45 6 146 105 aact:AA01183|5end_conserved_hypothetical_protein_810442..810672_POSITIVE 185 121 99 230 209 aact:AA01102|5end_pyrimidine_operon_regulatory_protein_760480..760710_POSITIVE 177 135 96 179 142 aact:AA01688|5end_conserved_hypothetical_protein;_possible_membrane_protein_1139635..1139865_POSITIVE 177 119 72 137 90 aact:AA00077|5end_conserved_hypothetical_protein_(possible_periplasmic_binding_transport_protein)_56111..56341_POSITIVE 174 49 26 63 34 aact:AA02910|5end_ribonuclease_E_2036137..2036367_POSITIVE 174 49 26 219 190 aact:AA01255|5end_hypothetical_protein_852656..852886_POSITIVE 174 128 81 142 94 aact:AA00288|5end_conserved_hypothetical_protein_200485..200715_POSITIVE 171 200 168 175 142 aact:AA02001|5end_conserved_hypothetical_protein_1359842..1360072_POSITIVE 170 148 106 70 30 aact:AA01834|5end_hypothetical_protein_1234640..1234870_POSITIVE 169 44 1 141 110 aact:AA00902|5end_RNA_polymerase_sigma-E_factor_sigma-24_623829..624059_POSITIVE 169 50 5 146 95 aact:AA00813|5end_hypothetical_protein_558807..559037_POSITIVE 168 144 102 151 121 aact:AA01848|5end_argininosuccinate_synthetase_1242278..1242508_POSITIVE 168 147 105 147 109 aact:AA01332|5end_galactosyltransferase_II_898875..899105_POSITIVE 167 127 98 141 115 aact:AA02774|5end_collagenase_prtC_homolog,_possible_protease_1936870..1937100_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 197 64 28 45 13 aact:AA01078|3end_transcriptional_regulator_(probable_MerR_family)_747453..747603_POSITIVE 195 45 6 134 93 aact:AA01182|3end_3-oxoacyl-(acyl-carrier-protein)_synthase_810510..810660_POSITIVE 191 147 101 109 60 aact:AA00817|3end_MutT-family_protein_562475..562625_POSITIVE 183 146 101 129 85 aact:AA01263|3end_hypothetical_protein_861086..861236_POSITIVE 177 135 96 125 88 aact:AA01687|3end_TldD_protein_1139661..1139811_POSITIVE 177 69 24 91 47 aact:AA00030|3end_hypothetical_protein_25168..25318_POSITIVE 174 128 81 84 36 aact:AA00287|3end_glutamate_5-kinase_(gamma-glutamyl_kinase)_200507..200657_POSITIVE 173 145 103 52 8 aact:AA01244|3end_transposase_848093..848243_POSITIVE 171 200 168 54 21 aact:AA02000|3end_2,3,4,5-tetrahydropyridine-2-carboxylate_1359801..1359951_POSITIVE 170 120 86 110 73 aact:AA01894|3end_conserved_hypothetical_protein_(possible_transthyretin-like_periplasmic_protein)_1279457..1279607_POSITIVE 169 50 5 109 58 aact:AA00812|3end_ATPase_(PP-loop_superfamily),_confers_aluminum_resistance_558850..559000_POSITIVE 168 187 141 142 102 aact:AA00807|3end_fumarate_reductase_13_kD_hydrophobic_protein_553188..553338_POSITIVE 167 127 98 107 81 aact:AA02773|3end_hypothetical_protein_1936916..1937066_POSITIVE 167 149 101 116 57 aact:AA00766|3end_heat_shock_protein_B25.3_(HSP-70_cofactor)_528039..528189_POSITIVE 167 48 6 63 18 aact:AA00461|3end_heat_shock_protein;_protease_322979..323129_POSITIVE 166 123 81 126 89 aact:AA00641|3end_cell_division_inhibitor_440130..440280_POSITIVE 164 139 95 126 83 aact:AA01876|3end_PTS_system,_IIBC_components_1264131..1264281_POSITIVE 163 147 104 141 97 aact:AA01854|3end_conserved_hypothetical_protein_1247089..1247239_POSITIVE 161 196 172 77 49 aact:AA01747|3end_single-stranded-DNA-specific_exonuclease_1183130..1183280_POSITIVE 160 44 7 133 94 aact:AA01811|3end_electron_transport_complex_protein_1221033..1221183_POSITIVE