Origin IGS: aattttctccttaagataatagacagttatctgttaaaggatagcaattagctgaaagtttgtatatagcaatccatcgctaaaaacactttaaaaaccaaccgcactttttgtcatagttaaaaatcctacgcaacatcaaaaaataaatttaatgtgttaaacgcattgactctgttcggactgcatagtagttttcactataataaaacttttccgtctttaaacggctacagtcatgtactcttgttggcgatattggaaaaattactgttgatctaaaaatgcaacttaaaaatggatcacattgatttacttggtgacgttagtggatggattacaggtgtattggctgagtacatcgcgtaaaatccggtacttttttactaatcctgtgcacaagggattctaatctgtaggtcgaaagttcgaatccttcacgacctatcaattagttattgacatcaaaattattaaattgactgctaactttttacttattatatctgcattcacacgagtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taactcgtgtgaatgcagatataataagtaaaaagttagcagtcaatttaataattttgatgtcaataactaattgataggtcgtgaaggattcgaactttcgacctacagattagaatcccttgtgcacaggattagtaaaaaagtaccggattttacgcgatgtactcagccaatacacctgtaatccatccactaacgtcaccaagtaaatcaatgtgatccatttttaagttgcatttttagatcaacagtaatttttccaatatcgccaacaagagtacatgactgtagccgtttaaagacggaaaagttttattatagtgaaaactactatgcagtccgaacagagtcaatgcgtttaacacattaaatttattttttgatgttgcgtaggatttttaactatgacaaaaagtgcggttggtttttaaagtgtttttagcgatggattgctatatacaaactttcagctaattgctatcctttaacagataactgtctattatcttaaggagaaaatt Mask Tandem Repeat Region ================================================ aattttctccttaagataatagacagttatctgttaaaggatagcaattagctgaaagtttgtatatagcaatccatcgctaaaaacactttaaaaaccaaccgcactttttgtcatagttaaaaatcctacgcaacatcaaaaaataaatttaatgtgttaaacgcattgactctgttcggactgcatagtagttttcactataataaaacttttccgtctttaaacggctacagtcatgtactcttgttggcgatattggaaaaattactgttgatctaaaaatgcaacttaaaaatggatcacattgatttacttggtgacgttagtggatggattacaggtgtattggctgagtacatcgcgtaaaatccggtacttttttactaatcctgtgcacaagggattctaatctgtaggtcgaaagttcgaatccttcacgacctatcaattagttattgacatcaaaattattaaattgactgctaactttttacttattatatctgcattcacacgagtta Find is-nt database================================================ Query_seq: AA02591:AA02592|AA02591:AA02592:conserved hypothetical protein (possible membrane protein):conserved hypothetical protein:->->:1802647..1803170 524 aattttctccttaagataatagacagttatctgttaaaggatagcaattagctgaaagtttgtatatagcaatccatcgctaaaaacactttaaaaaccaaccgcactttttgtcatagttaaaaatcctacgcaacatcaaaaaataaatttaatgtgttaaacgcattgactctgttcggactgcatagtagttttcactataataaaacttttccgtctttaaacggctacagtcatgtactcttgttggcgatattggaaaaattactgttgatctaaaaatgcaacttaaaaatggatcacattgatttacttggtgacgttagtggatggattacaggtgtattggctgagtacatcgcgtaaaatccggtacttttttactaatcctgtgcacaagggattctaatctgtaggtcgaaagttcgaatccttcacgacctatcaattagttattgacatcaaaattattaaattgactgctaactttttacttattatatctgcattcacacgagtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02591:AA02592|AA02591:AA02592:conserved hypothetical protein (possible membrane protein):conserved hypothetical protein:->->:1802647..1803170 524 aattttctccttaagataatagacagttatctgttaaaggatagcaattagctgaaagtttgtatatagcaatccatcgctaaaaacactttaaaaaccaaccgcactttttgtcatagttaaaaatcctacgcaacatcaaaaaataaatttaatgtgttaaacgcattgactctgttcggactgcatagtagttttcactataataaaacttttccgtctttaaacggctacagtcatgtactcttgttggcgatattggaaaaattactgttgatctaaaaatgcaacttaaaaatggatcacattgatttacttggtgacgttagtggatggattacaggtgtattggctgagtacatcgcgtaaaatccggtacttttttactaatcctgtgcacaagggattctaatctgtaggtcgaaagttcgaatccttcacgacctatcaattagttattgacatcaaaattattaaattgactgctaactttttacttattatatctgcattcacacgagtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02591:AA02592|AA02591:AA02592:conserved hypothetical protein (possible membrane protein):conserved hypothetical protein:->->:1802647..1803170 524 aattttctccttaagataatagacagttatctgttaaaggatagcaattagctgaaagtttgtatatagcaatccatcgctaaaaacactttaaaaaccaaccgcactttttgtcatagttaaaaatcctacgcaacatcaaaaaataaatttaatgtgttaaacgcattgactctgttcggactgcatagtagttttcactataataaaacttttccgtctttaaacggctacagtcatgtactcttgttggcgatattggaaaaattactgttgatctaaaaatgcaacttaaaaatggatcacattgatttacttggtgacgttagtggatggattacaggtgtattggctgagtacatcgcgtaaaatccggtacttttttactaatcctgtgcacaagggattctaatctgtaggtcgaaagttcgaatccttcacgacctatcaattagttattgacatcaaaattattaaattgactgctaactttttacttattatatctgcattcacacgagtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aattttctccttaagataatagacagttatctgttaaaggatagcaattagctgaaagtttgtatatagcaatccatcgctaaaaacactttaaaaaccaaccgcactttttgtcatagttaaaaatcctacgcaacatcaaaaaataaatttaatgtgttaaacgcattgactctgttcggactgcatagtagttttcactataataaaacttttccgtctttaaacggctacagtcatgtactcttgttggcgatattggaaaaattactgttgatctaaaaatgcaacttaaaaatggatcacattgatttacttggtgacgttagtggatggattacaggtgtattggctgagtacatcgcgtaaaatccggtacttttttactaatcctgtgcacaagggattctaatctgtaggtcgaaagttcgaatccttcacgacctatcaattagttattgacatcaaaattattaaattgactgctaactttttacttattatatctgcattcacacgagtta Predict ORF larger than 30AA ================================================ Protein_Len: 41 Strand: + Start: 157 End: 279 ............................................................................................................................................................ M L N A L T L F G L H S S F H Y N K T F P S L N G Y S H V L L L A I L E K L L L I ..................................................................................................................................................................................................................................................... Protein_Len: 36 Strand: + Start: 302 End: 409 ............................................................................................................................................................................................................................................................................................................. M T L I Y L V T L V D G L Q V Y W L S T S R K I R Y F F T N P V H K G F ................................................................................................................... Protein_Len: 37 Strand: - Start: 341 End: 451 .................................................................................................................................................................................................................................................................................................................................................... L H I P Q T C R T F D P V K K S I R H V L S E L R Y T S L E F G E R G I M ......................................................................... Protein_Len: 44 Strand: - Start: 155 End: 286 .......................................................................................................................................................... H T L R M S E T R V A Y Y N E S Y Y F K E T K L R S C D H V R T P S I P F I V T S R F M .............................................................................................................................................................................................................................................. aattttctccttaagataatagacagttatctgttaaaggatagcaattagctgaaagtttgtatatagcaatccatcgctaaaaacactttaaaaaccaaccgcactttttgtcatagttaaaaatcctacgcaacatcaaaaaataaatttaatgtgttaaacgcattgactctgttcggactgcatagtagttttcactataataaaacttttccgtctttaaacggctacagtcatgtactcttgttggcgatattggaaaaattactgttgatctaaaaatgcaacttaaaaatggatcacattgatttacttggtgacgttagtggatggattacaggtgtattggctgagtacatcgcgtaaaatccggtacttttttactaatcctgtgcacaagggattctaatctgtaggtcgaaagttcgaatccttcacgacctatcaattagttattgacatcaaaattattaaattgactgctaactttttacttattatatctgcattcacacgagtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aattttctccttaagataatagacagttatctgttaaaggatagcaattagctgaaagtttgtatatagcaatccatcgctaaaaacactttaaaaaccaaccgcactttttgtcatagttaaaaatcctacgcaacatcaaaaaataaatttaatgtgttaaacgcattgactctgttcggactgcatagtagttttcactataataaaacttttccgtctttaaacggctacagtcatgtactcttgttggcgatattggaaaaattactgttgatctaaaaatgcaacttaaaaatggatcacattgatttacttggtgacgttagtggatggattacaggtgtattggctgagtacatcgcgtaaaatccggtacttttttactaatcctgtgcacaagggattctaatctgtaggtcgaaagttcgaatccttcacgacctatcaattagttattgacatcaaaattattaaattgactgctaactttttacttattatatctgcattcacacgagtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02591:AA02592|AA02591:AA02592:conserved hypothetical protein (possible membrane protein):conserved hypothetical protein:->->:1802647..1803170 524 aattttctccttaagataatagacagttatctgttaaaggatagcaattagctgaaagtttgtatatagcaatccatcgctaaaaacactttaaaaaccaaccgcactttttgtcatagttaaaaatcctacgcaacatcaaaaaataaatttaatgtgttaaacgcattgactctgttcggactgcatagtagttttcactataataaaacttttccgtctttaaacggctacagtcatgtactcttgttggcgatattggaaaaattactgttgatctaaaaatgcaacttaaaaatggatcacattgatttacttggtgacgttagtggatggattacaggtgtattggctgagtacatcgcgtaaaatccggtacttttttactaatcctgtgcacaagggattctaatctgtaggtcgaaagttcgaatccttcacgacctatcaattagttattgacatcaaaattattaaattgactgctaactttttacttattatatctgcattcacacgagtta Intra-Species Hit: Count: 1 Min: 1 Max: 524 Len: 524 Subject: aact_AA02591_AA02592|conserved hypothetical protein (possible membrane protein):conserved hypothetical protein|POSITIVE:POSITIVE|[1802647,1803170]|524 HSP 1 e-value: 0.0 bit: 1039.0 Len: 524 Query Start:1 Query End:524 Subject Strand: POSITIVE Subject Start: 1 Subject End: 524 aattttctccttaagataatagacagttatctgttaaaggatagcaattagctgaaagtttgtatatagcaatccatcgctaaaaacactttaaaaaccaaccgcactttttgtcatagttaaaaatcctacgcaacatcaaaaaataaatttaatgtgttaaacgcattgactctgttcggactgcatagtagttttcactataataaaacttttccgtctttaaacggctacagtcatgtactcttgttggcgatattggaaaaattactgttgatctaaaaatgcaacttaaaaatggatcacattgatttacttggtgacgttagtggatggattacaggtgtattggctgagtacatcgcgtaaaatccggtacttttttactaatcctgtgcacaagggattctaatctgtaggtcgaaagttcgaatccttcacgacctatcaattagttattgacatcaaaattattaaattgactgctaactttttacttattatatctgcattcacacgagtta aattttctccttaagataatagacagttatctgttaaaggatagcaattagctgaaagtttgtatatagcaatccatcgctaaaaacactttaaaaaccaaccgcactttttgtcatagttaaaaatcctacgcaacatcaaaaaataaatttaatgtgttaaacgcattgactctgttcggactgcatagtagttttcactataataaaacttttccgtctttaaacggctacagtcatgtactcttgttggcgatattggaaaaattactgttgatctaaaaatgcaacttaaaaatggatcacattgatttacttggtgacgttagtggatggattacaggtgtattggctgagtacatcgcgtaaaatccggtacttttttactaatcctgtgcacaagggattctaatctgtaggtcgaaagttcgaatccttcacgacctatcaattagttattgacatcaaaattattaaattgactgctaactttttacttattatatctgcattcacacgagtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUUUCUCCUUAAGAUAAUAGACAGUUAUCUGUUAAAGGAUAGCAAUUAGCUGAAAGUUUGUAUAUAGCAAUCCAUCGCUAAAAACACUUUAAAAACCAACCGCACUUUUUGUCAUAGUUAAAAAUCCUACGCAACAUCAAAAAAUAAAUUUAAUGUGUUAAACGCAUUGACUCUGUUCGGACUGCAUAGUAGUUUUCACUAUAAUAAAACUUUUCCGUCUUUAAACGGCUACAGUCAUGUACUCUUGUUGGCGAUAUUGGAAAAAUUACUGUUGAUCUAAAAAUGCAACUUAAAAAUGGAUCACAUUGAUUUACUUGGUGACGUUAGUGGAUGGAUUACAGGUGUAUUGGCUGAGUACAUCGCGUAAAAUCCGGUACUUUUUUACUAAUCCUGUGCACAAGGGAUUCUAAUCUGUAGGUCGAAAGUUCGAAUCCUUCACGACCUAUCAAUUAGUUAUUGACAUCAAAAUUAUUAAAUUGACUGCUAACUUUUUACUUAUUAUAUCUGCAUUCACACGAGUUA ......(((....(((((((.....)))))))......(((((..(((.((..(((((((.(((..((((........))))....((.(((((..............((((((.(((((.((((...(((.(((................((((((((.....)))))))).(((....))).))).)))...)))).))))).)))))).....((((......)))).....((((....(((((((..(((.....(((.((((((.(((.((((((.................))))))))).))))))...((((((....((((..((((((((.((((((((.....))))))))..)))..))))).))))...)))))).)))..))))))))))...(((((..((((((((.(((........)))..))))))))..)))))....))))..........))))).))..))).)))))))..)).))).)))))..........)))... (-108.33) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACUCGUGUGAAUGCAGAUAUAAUAAGUAAAAAGUUAGCAGUCAAUUUAAUAAUUUUGAUGUCAAUAACUAAUUGAUAGGUCGUGAAGGAUUCGAACUUUCGACCUACAGAUUAGAAUCCCUUGUGCACAGGAUUAGUAAAAAAGUACCGGAUUUUACGCGAUGUACUCAGCCAAUACACCUGUAAUCCAUCCACUAACGUCACCAAGUAAAUCAAUGUGAUCCAUUUUUAAGUUGCAUUUUUAGAUCAACAGUAAUUUUUCCAAUAUCGCCAACAAGAGUACAUGACUGUAGCCGUUUAAAGACGGAAAAGUUUUAUUAUAGUGAAAACUACUAUGCAGUCCGAACAGAGUCAAUGCGUUUAACACAUUAAAUUUAUUUUUUGAUGUUGCGUAGGAUUUUUAACUAUGACAAAAAGUGCGGUUGGUUUUUAAAGUGUUUUUAGCGAUGGAUUGCUAUAUACAAACUUUCAGCUAAUUGCUAUCCUUUAACAGAUAACUGUCUAUUAUCUUAAGGAGAAAAUU ..(((.(.((((.(((...........)))....(((((.......((((.((((((((.((((((...((((((..(((((((.((((........)))))))))))..))))))......))).))))))))))).))))........((((.(((((.(.((((.........))))).)))))...)))).))))).))))).)))........(..(((((((.(((((..(((((((.((((((((.(((.(((((...(((..................(((((((.(((((....))))).............((((....))))...))))))).....((((((..(((((.....((((((((.......)))))))).))))).))))))....)))...))))).))).))))))))..))))))).))))).)))))))..)...............(((.....))).(((((....((((((.......)))))).)))))....... (-113.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 430 119 76 167 125 aact:AA02673|5end_gluconate_utilization_operon_repressor_1858913..1859143_POSITIVE 393 109 66 93 43 aact:AA01641|5end_hypothetical_protein_1110819..1111049_POSITIVE 375 113 74 80 41 aact:AA02123|5end_hypothetical_protein_1448244..1448474_POSITIVE 360 114 73 197 155 aact:AA00268|5end_acyl_carrier_protein_(ACP)_188104..188334_POSITIVE 360 114 73 46 4 aact:AA00267|5end_hypothetical_protein_187953..188183_POSITIVE 357 370 321 78 35 aact:AA02316|5end_exodeoxyribonuclease_VII_large_subunit_1581682..1581912_POSITIVE 354 115 72 155 102 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 351 358 318 61 14 aact:AA01787|5end_conserved_hypothetical_protein_1205292..1205522_POSITIVE 342 398 352 51 3 aact:AA01421|5end_phosphoglycolate_phosphatase_955055..955285_POSITIVE 342 110 69 121 84 aact:AA01308|5end_cyclic_AMP_receptor_protein_888044..888274_POSITIVE 340 140 93 227 180 aact:AA01471|5end_type_I_restriction_enzyme_M_(modification_chain)_987321..987551_POSITIVE 338 124 82 139 95 aact:AA02252|5end_conserved_hypothetical_protein_1533840..1534070_POSITIVE 329 115 75 127 87 aact:AA00552|5end_periplasmic_nitrate_reductase_precursor_377331..377561_POSITIVE 324 138 99 139 104 aact:AA02703|5end_hypothetical_protein_1885365..1885595_POSITIVE 324 116 75 197 155 aact:AA01635|5end_ribonucleoside_diphosphate_reductase,_beta_chain_1104615..1104845_POSITIVE 322 115 71 151 95 aact:AA01008|5end_mazG_beta-lactamase_regulatory_protein_homolog_703470..703700_POSITIVE 321 359 318 173 132 aact:AA01756|5end_cobalt_membrane_transport_protein_1186475..1186705_POSITIVE 321 115 81 135 97 aact:AA01436|5end_fructose-bisphosphate_aldolase_class_II_962981..963211_POSITIVE 319 110 65 180 140 aact:AA00561|5end_DNA_transformation_protein_384584..384814_POSITIVE 317 116 74 168 116 aact:AA01883|5end_recycling_protein;_permease_1269821..1270051_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 430 119 76 91 49 aact:AA02671|3end_gluconokinase_1858917..1859067_POSITIVE 374 377 333 130 83 aact:AA02865|3end_iron-regulated_outer_membrane_protein_(omp64)_2012542..2012692_POSITIVE 363 116 79 132 93 aact:AA01937|3end_sugar_fermentation_stimulation_protein_1309334..1309484_POSITIVE 357 370 321 68 25 aact:AA02315|3end_conserved_hypothetical_protein_1581752..1581902_POSITIVE 354 115 72 150 97 aact:AA00323|3end_conserved_hypothetical_protein_(possible_SAM-dependent_methyltransferase)_220222..220372_POSITIVE 338 124 82 62 18 aact:AA02251|3end_biotin_sulfoxide_reductase;_Trimethylamine-N-oxide_reductase_precursor_1533843..1533993_POSITIVE 331 118 82 41 3 aact:AA02540|3end_5-methyltetrahydropteroyltriglutamate-homocystein_e_methyltransferase_1769499..1769649_POSITIVE 329 115 75 119 79 aact:AA00551|3end_hypothetical_protein_377403..377553_POSITIVE 324 138 99 79 44 aact:AA02702|3end_succinyl-CoA_synthetase,_beta_chain_1885385..1885535_POSITIVE 321 115 81 95 57 aact:AA01434|3end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_963021..963171_POSITIVE 319 110 65 107 67 aact:AA00560|3end_RNA_polymerase_sigma-32_factor_384591..384741_POSITIVE 317 112 81 147 116 aact:AA01823|3end_hypothetical_protein_1227747..1227897_POSITIVE 317 112 81 71 40 aact:AA01822|3end_lysostaphin_precursor_1227671..1227821_POSITIVE 313 115 78 41 1 aact:AA01019|3end_conserved_hypothetical_protein_712719..712869_POSITIVE 311 359 318 66 18 aact:AA02916|3end_2-hydroxyacid_dehydrogenase;_D-lactate_dehydrogenase_2042147..2042297_POSITIVE 311 127 81 88 35 aact:AA02136|3end_aspartate_aminotransferase_1460476..1460626_POSITIVE 308 139 94 113 74 aact:AA00973|3end_transcriptional_regulator,_DeoR_family_679237..679387_POSITIVE 304 202 165 80 38 aact:AA02432|3end_penicillin-binding_protein_1B_1678325..1678475_POSITIVE 303 116 79 81 41 aact:AA01457|3end_D-xylose_ABC_transporter,_membrane_spanning_protein_978808..978958_POSITIVE 299 110 67 107 70 aact:AA02046|3end_PTS_system_galactitol-specific_enzyme_IIC_1393687..1393837_POSITIVE