Origin IGS: attaattccttaagttaattatattttctaaaaacatcttaaaaaacaaccgcactttttcacattaacatgcaaaacgtggttatttcctttccccttgatagcgcacgcttcatagcgtgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcgccatcaaaaaataccttgacggttgcgtccacatttagaccaatgcaaacacaaacgtgaacgcttgcgctatcatgggagcaaaaacgtggtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaccacgtttttgctcccatgatagcgcaagcgttcacgtttgtgtttgcattggtctaaatgtggacgcaaccgtcaaggtattttttgatggcgcgcgtttcctaacgcgtgcctgtaatatcaataagttaaaagcacacgctatgaagcgtgcgctatcaaggggaaaggaaataaccacgttttgcatgttaatgtgaaaaagtgcggttgttttttaagatgtttttagaaaatataattaacttaaggaattaat Mask Tandem Repeat Region ================================================ attaattccttaagttaattatattttctaaaaacatcttaaaaaacaaccgcactttttcacattaacatgcaaaacgtggttatttcctttccccttgatagcgcacgcttcatagcgtgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcgccatcaaaaaataccttgacggttgcgtccacatttagaccaatgcaaacacaaacgtgaacgcttgcgctatcatgggagcaaaaacgtggtta Find is-nt database================================================ Query_seq: AA02570:AA02573|AA02570:AA02573:conserved hypothetical protein:ABC transporter, ATP-binding protein:->->:1790286..1790548 263 attaattccttaagttaattatattttctaaaaacatcttaaaaaacaaccgcactttttcacattaacatgcaaaacgtggttatttcctttccccttgatagcgcacgcttcatagcgtgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcgccatcaaaaaataccttgacggttgcgtccacatttagaccaatgcaaacacaaacgtgaacgcttgcgctatcatgggagcaaaaacgtggtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02570:AA02573|AA02570:AA02573:conserved hypothetical protein:ABC transporter, ATP-binding protein:->->:1790286..1790548 263 attaattccttaagttaattatattttctaaaaacatcttaaaaaacaaccgcactttttcacattaacatgcaaaacgtggttatttcctttccccttgatagcgcacgcttcatagcgtgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcgccatcaaaaaataccttgacggttgcgtccacatttagaccaatgcaaacacaaacgtgaacgcttgcgctatcatgggagcaaaaacgtggtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02570:AA02573|AA02570:AA02573:conserved hypothetical protein:ABC transporter, ATP-binding protein:->->:1790286..1790548 263 attaattccttaagttaattatattttctaaaaacatcttaaaaaacaaccgcactttttcacattaacatgcaaaacgtggttatttcctttccccttgatagcgcacgcttcatagcgtgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcgccatcaaaaaataccttgacggttgcgtccacatttagaccaatgcaaacacaaacgtgaacgcttgcgctatcatgggagcaaaaacgtggtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attaattccttaagttaattatattttctaaaaacatcttaaaaaacaaccgcactttttcacattaacatgcaaaacgtggttatttcctttccccttgatagcgcacgcttcatagcgtgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcgccatcaaaaaataccttgacggttgcgtccacatttagaccaatgcaaacacaaacgtgaacgcttgcgctatcatgggagcaaaaacgtggtta Predict ORF larger than 30AA ================================================ Protein_Len: 54 Strand: + Start: 64 End: 225 ............................................................... M N M Q N V V I S F P L D S A R F I A C A F N L L I L Q A R V R K R A P S K N T L T V A S T F R P M Q T Q T ...................................... Protein_Len: 50 Strand: - Start: 63 End: 212 .............................................................. M L M C F T T I E K G R S L A R K M A H A K L K N I N C A R T L F R A G D F F V K V T A D V N L G M ................................................... attaattccttaagttaattatattttctaaaaacatcttaaaaaacaaccgcactttttcacattaacatgcaaaacgtggttatttcctttccccttgatagcgcacgcttcatagcgtgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcgccatcaaaaaataccttgacggttgcgtccacatttagaccaatgcaaacacaaacgtgaacgcttgcgctatcatgggagcaaaaacgtggtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attaattccttaagttaattatattttctaaaaacatcttaaaaaacaaccgcactttttcacattaacatgcaaaacgtggttatttcctttccccttgatagcgcacgcttcatagcgtgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcgccatcaaaaaataccttgacggttgcgtccacatttagaccaatgcaaacacaaacgtgaacgcttgcgctatcatgggagcaaaaacgtggtta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -12.3 Tail_Score: -4.36729 Start: 104 End: 125 Full_Region: CCTTTCCCCTTGATA GCGCACGCT TCAT AGCGTGTGC TTTTAACTTATTGAT .......................................................................................................GCGCACGCTTCATAGCGTGTGC.......................................................................................................................................... TransTerm Strand: - Conf: 95 HP_score: -14.6 Tail_Score: -2.8655 Start: 147 End: 170 Full_Region: aaggtattttttgat ggcgcgcgtt tcct aacgcgtgcc tgtaatatcaataag ..................................................................................................................................................ggcgcgcgtttcctaacgcgtgcc............................................................................................. TransTerm Strand: - Conf: 57 HP_score: -2.2 Tail_Score: -4.20707 Start: 182 End: 231 Full_Region: ccatgatagcgcaag cgttcacgtttgtgtttgcatt ggtcta aatgtggacgcaaccgtcaagg tattttttgatggcg .....................................................................................................................................................................................cgttcacgtttgtgtttgcattggtctaaatgtggacgcaaccgtcaagg................................ Find igs database================================================ Query_seq: AA02570:AA02573|AA02570:AA02573:conserved hypothetical protein:ABC transporter, ATP-binding protein:->->:1790286..1790548 263 attaattccttaagttaattatattttctaaaaacatcttaaaaaacaaccgcactttttcacattaacatgcaaaacgtggttatttcctttccccttgatagcgcacgcttcatagcgtgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcgccatcaaaaaataccttgacggttgcgtccacatttagaccaatgcaaacacaaacgtgaacgcttgcgctatcatgggagcaaaaacgtggtta Intra-Species Hit: Count: 1 Min: 1 Max: 263 Len: 263 Subject: aact_AA02570_AA02573|conserved hypothetical protein:ABC transporter, ATP-binding protein|POSITIVE:POSITIVE|[1790286,1790548]|263 HSP 1 e-value: 1.0E-147 bit: 521.0 Len: 263 Query Start:1 Query End:263 Subject Strand: POSITIVE Subject Start: 1 Subject End: 263 attaattccttaagttaattatattttctaaaaacatcttaaaaaacaaccgcactttttcacattaacatgcaaaacgtggttatttcctttccccttgatagcgcacgcttcatagcgtgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcgccatcaaaaaataccttgacggttgcgtccacatttagaccaatgcaaacacaaacgtgaacgcttgcgctatcatgggagcaaaaacgtggtta attaattccttaagttaattatattttctaaaaacatcttaaaaaacaaccgcactttttcacattaacatgcaaaacgtggttatttcctttccccttgatagcgcacgcttcatagcgtgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcgccatcaaaaaataccttgacggttgcgtccacatttagaccaatgcaaacacaaacgtgaacgcttgcgctatcatgggagcaaaaacgtggtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUAAUUCCUUAAGUUAAUUAUAUUUUCUAAAAACAUCUUAAAAAACAACCGCACUUUUUCACAUUAACAUGCAAAACGUGGUUAUUUCCUUUCCCCUUGAUAGCGCACGCUUCAUAGCGUGUGCUUUUAACUUAUUGAUAUUACAGGCACGCGUUAGGAAACGCGCGCCAUCAAAAAAUACCUUGACGGUUGCGUCCACAUUUAGACCAAUGCAAACACAAACGUGAACGCUUGCGCUAUCAUGGGAGCAAAAACGUGGUUA (((((((.....)))))))............................((((((...............((((.....))))..........(((((..((((((((((.((((((((((((((((((.(((..........))).))))))))))).(((..((((.(((.((((........)))).))))))))))..........................))))).)).)))))))))).))))).......)))))). (-68.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACCACGUUUUUGCUCCCAUGAUAGCGCAAGCGUUCACGUUUGUGUUUGCAUUGGUCUAAAUGUGGACGCAACCGUCAAGGUAUUUUUUGAUGGCGCGCGUUUCCUAACGCGUGCCUGUAAUAUCAAUAAGUUAAAAGCACACGCUAUGAAGCGUGCGCUAUCAAGGGGAAAGGAAAUAACCACGUUUUGCAUGUUAAUGUGAAAAAGUGCGGUUGUUUUUUAAGAUGUUUUUAGAAAAUAUAAUUAACUUAAGGAAUUAAU ...((..........((((.(((((((((..((....(((.(((((((..((((......))))..))))))).)))...((((((.......((((((((((....))))))))))...))))))............))..(((((....)))))))))))))).))))..(((((((((((.(((((..(((....)))..)))...)).)))))))))))...((((((.....))))))...........))....... (-87.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 471 173 147 181 152 aact:AA02212|5end_ribonucleoside-diphosphate_reductase_beta_chain_1506012..1506242_POSITIVE 441 190 147 162 107 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 406 169 121 171 123 aact:AA02450|5end_conserved_hypothetical_protein_1687701..1687931_POSITIVE 399 76 31 140 95 aact:AA00792|5end_conserved_hypothetical_protein_543142..543372_POSITIVE 386 150 103 68 23 aact:AA02449|5end_hypothetical_protein_1687575..1687805_POSITIVE 383 127 90 142 102 aact:AA02570|5end_conserved_hypothetical_protein_1789150..1789380_POSITIVE 349 150 101 174 120 aact:AA01770|5end_hypothetical_protein_1193111..1193341_POSITIVE 346 133 97 159 122 aact:AA01982|5end_maltodextrin_phosphorylase_1339387..1339617_POSITIVE 345 160 112 197 148 aact:AA02446|5end_conserved_hypothetical_protein_1686841..1687071_POSITIVE 343 129 81 171 128 aact:AA00042|5end_hypothetical_protein_33810..34040_POSITIVE 338 190 144 122 81 aact:AA01432|5end_lysophospholipase_L2_960715..960945_POSITIVE 337 174 142 188 160 aact:AA01774|5end_5,10-methylenetetrahydrofolate_reductase_1195015..1195245_POSITIVE 337 174 142 50 22 aact:AA01773|5end_hypothetical_protein_1194877..1195107_POSITIVE 335 200 159 52 2 aact:AA01424|5end_threonine_deaminase_(dehydratase)_957740..957970_POSITIVE 333 199 152 190 134 aact:AA00201|5end_prolipoprotein_diacylglyceryl_transferase_139740..139970_POSITIVE 327 67 23 139 98 aact:AA02047|5end_galactitol-1-phosphate_5-dehydrogenase_1393633..1393863_POSITIVE 326 197 152 227 166 aact:AA01054|5end_conserved_hypothetical_protein_733678..733908_POSITIVE 322 190 141 141 90 aact:AA02950|5end_isoleucyl-tRNA_synthetase_2062295..2062525_POSITIVE 322 250 207 137 100 aact:AA02697|5end_hydrogenase_3_maturation_protease_1879870..1880100_POSITIVE 319 238 191 61 10 aact:AA02374|5end_ribose_5-phosphate_isomerase_A_1625839..1626069_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 471 173 147 45 16 aact:AA02210|3end_ribonucleoside-diphosphate_reductase_alpha_chain_1505956..1506106_POSITIVE 441 190 147 79 24 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 425 193 154 126 87 aact:AA01660|3end_hypothetical_protein_1125282..1125432_POSITIVE 399 76 31 52 7 aact:AA00790|3end_recombination_DNA_repair_protein_543134..543284_POSITIVE 383 127 90 96 56 aact:AA02569|3end_hypothetical_protein_1789184..1789334_POSITIVE 366 70 30 46 3 aact:AA02540|3end_5-methyltetrahydropteroyltriglutamate-homocystein_e_methyltransferase_1769499..1769649_POSITIVE 349 150 101 115 61 aact:AA01769|3end_hypothetical_protein_1193132..1193282_POSITIVE 347 126 89 57 22 aact:AA01169|3end_hypothetical_protein_802229..802379_POSITIVE 346 133 97 46 9 aact:AA01981|3end_4-alpha-glucanotransferase_1339354..1339504_POSITIVE 343 129 81 98 55 aact:AA00041|3end_cytochrome_c-type_biogenesis_protein_33817..33967_POSITIVE 338 70 28 142 94 aact:AA01937|3end_sugar_fermentation_stimulation_protein_1309334..1309484_POSITIVE 338 190 144 78 37 aact:AA01431|3end_conserved_hypothetical_protein_(possible_hydrolase)_960751..960901_POSITIVE 337 174 142 35 7 aact:AA01772|3end_shikimate_5-dehydrogenase_protein_1194942..1195092_POSITIVE 333 199 152 139 83 aact:AA00200|3end_thymidylate_synthase_139769..139919_POSITIVE 328 179 136 79 30 aact:AA01173|3end_VapD-homolog_804385..804535_POSITIVE 327 67 23 113 72 aact:AA02046|3end_PTS_system_galactitol-specific_enzyme_IIC_1393687..1393837_POSITIVE 322 250 207 144 107 aact:AA02696|3end_formate_hydrogenlyase_maturation_protein_1879957..1880107_POSITIVE 319 250 211 135 91 aact:AA01840|3end_conserved_hypothetical_protein_1237554..1237704_POSITIVE 319 138 94 73 38 aact:AA01054|3end_conserved_hypothetical_protein_733940..734090_POSITIVE 318 73 31 66 19 aact:AA02251|3end_biotin_sulfoxide_reductase;_Trimethylamine-N-oxide_reductase_precursor_1533843..1533993_POSITIVE