Origin IGS: aattttctcctttttgaggttaattgattcagcaactgagatgcagtctatgacggaacagcagggggatcaatgcacaggtttgaaaactgtgatcaggacgacattttgagaaaggcttccgaaggtataagaacaggaatgacatctgatggttcaagcgtccacgcttggaccgacacaagcaaaagcgcggatgcttgcgctatcatgggagctatcctggtggactggaattacggttggtgagcttgtcgaaccatgagtaattccggctctcgagcaacataaaactcatgcttcgactaacccttcgaaaagctcaggaaccgtctcagcaaccgttttgtgtattaaccggattat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ataatccggttaatacacaaaacggttgctgagacggttcctgagcttttcgaagggttagtcgaagcatgagttttatgttgctcgagagccggaattactcatggttcgacaagctcaccaaccgtaattccagtccaccaggatagctcccatgatagcgcaagcatccgcgcttttgcttgtgtcggtccaagcgtggacgcttgaaccatcagatgtcattcctgttcttataccttcggaagcctttctcaaaatgtcgtcctgatcacagttttcaaacctgtgcattgatccccctgctgttccgtcatagactgcatctcagttgctgaatcaattaacctcaaaaaggagaaaatt Mask Tandem Repeat Region ================================================ aattttctcctttttgaggttaattgattcagcaactgagatgcagtctatgacggaacagcagggggatcaatgcacaggtttgaaaactgtgatcaggacgacattttgagaaaggcttccgaaggtataagaacaggaatgacatctgatggttcaagcgtccacgcttggaccgacacaagcaaaagcgcggatgcttgcgctatcatgggagctatcctggtggactggaattacggttggtgagcttgtcgaaccatgagtaattccggctctcgagcaacataaaactcatgcttcgactaacccttcgaaaagctcaggaaccgtctcagcaaccgttttgtgtattaaccggattat Find is-nt database================================================ Query_seq: AA02568:AA02569|AA02568:AA02569:sugar phosphotransferase component II B:hypothetical protein:->->:1788786..1789151 366 aattttctcctttttgaggttaattgattcagcaactgagatgcagtctatgacggaacagcagggggatcaatgcacaggtttgaaaactgtgatcaggacgacattttgagaaaggcttccgaaggtataagaacaggaatgacatctgatggttcaagcgtccacgcttggaccgacacaagcaaaagcgcggatgcttgcgctatcatgggagctatcctggtggactggaattacggttggtgagcttgtcgaaccatgagtaattccggctctcgagcaacataaaactcatgcttcgactaacccttcgaaaagctcaggaaccgtctcagcaaccgttttgtgtattaaccggattat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02568:AA02569|AA02568:AA02569:sugar phosphotransferase component II B:hypothetical protein:->->:1788786..1789151 366 aattttctcctttttgaggttaattgattcagcaactgagatgcagtctatgacggaacagcagggggatcaatgcacaggtttgaaaactgtgatcaggacgacattttgagaaaggcttccgaaggtataagaacaggaatgacatctgatggttcaagcgtccacgcttggaccgacacaagcaaaagcgcggatgcttgcgctatcatgggagctatcctggtggactggaattacggttggtgagcttgtcgaaccatgagtaattccggctctcgagcaacataaaactcatgcttcgactaacccttcgaaaagctcaggaaccgtctcagcaaccgttttgtgtattaaccggattat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02568:AA02569|AA02568:AA02569:sugar phosphotransferase component II B:hypothetical protein:->->:1788786..1789151 366 aattttctcctttttgaggttaattgattcagcaactgagatgcagtctatgacggaacagcagggggatcaatgcacaggtttgaaaactgtgatcaggacgacattttgagaaaggcttccgaaggtataagaacaggaatgacatctgatggttcaagcgtccacgcttggaccgacacaagcaaaagcgcggatgcttgcgctatcatgggagctatcctggtggactggaattacggttggtgagcttgtcgaaccatgagtaattccggctctcgagcaacataaaactcatgcttcgactaacccttcgaaaagctcaggaaccgtctcagcaaccgttttgtgtattaaccggattat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aattttctcctttttgaggttaattgattcagcaactgagatgcagtctatgacggaacagcagggggatcaatgcacaggtttgaaaactgtgatcaggacgacattttgagaaaggcttccgaaggtataagaacaggaatgacatctgatggttcaagcgtccacgcttggaccgacacaagcaaaagcgcggatgcttgcgctatcatgggagctatcctggtggactggaattacggttggtgagcttgtcgaaccatgagtaattccggctctcgagcaacataaaactcatgcttcgactaacccttcgaaaagctcaggaaccgtctcagcaaccgttttgtgtattaaccggattat Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 2 End: 109 . M F S F L R L I D S A T E M Q S M T E Q Q G D Q C T G L K T V I R T T F ................................................................................................................................................................................................................................................................. Protein_Len: 58 Strand: + Start: 73 End: 246 ........................................................................ M H R F E N C D Q D D I L R K A S E G I R T G M T S D G S S V H A W T D T S K S A D A C A I M G A I L V D W N Y G W ........................................................................................................................ Protein_Len: 38 Strand: + Start: 149 End: 262 .................................................................................................................................................... M M V Q A S T L G P T Q A K A R M L A L S W E L S W W T G I T V G E L V E P ........................................................................................................ Protein_Len: 32 Strand: + Start: 171 End: 266 .......................................................................................................................................................................... M D R H K Q K R G C L R Y H G S Y P G G L E L R L V S L S N H E .................................................................................................... Protein_Len: 32 Strand: + Start: 269 End: 364 ............................................................................................................................................................................................................................................................................ M P A L E Q H K T H A S T N P S K S S G T V S A T V L C I N R I .. Protein_Len: 37 Strand: - Start: 160 End: 270 ............................................................................................................................................................... A D V S P G V C A F A R I S A S D H S S D Q H V P I V T P S S T S G H T M ................................................................................................ Protein_Len: 41 Strand: - Start: 99 End: 221 .................................................................................................. S S M K L F A E S P I L V P I V D S P E L T W A Q V S V L L L A S A Q A I M P A M ................................................................................................................................................. Protein_Len: 51 Strand: - Start: 32 End: 184 ............................... C S L H L R H R F L L P I L A C T Q F S H D P R C K S F P K R L Y L F L F S M Q H N L R G R K S R C M ...................................................................................................................................................................................... aattttctcctttttgaggttaattgattcagcaactgagatgcagtctatgacggaacagcagggggatcaatgcacaggtttgaaaactgtgatcaggacgacattttgagaaaggcttccgaaggtataagaacaggaatgacatctgatggttcaagcgtccacgcttggaccgacacaagcaaaagcgcggatgcttgcgctatcatgggagctatcctggtggactggaattacggttggtgagcttgtcgaaccatgagtaattccggctctcgagcaacataaaactcatgcttcgactaacccttcgaaaagctcaggaaccgtctcagcaaccgttttgtgtattaaccggattat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aattttctcctttttgaggttaattgattcagcaactgagatgcagtctatgacggaacagcagggggatcaatgcacaggtttgaaaactgtgatcaggacgacattttgagaaaggcttccgaaggtataagaacaggaatgacatctgatggttcaagcgtccacgcttggaccgacacaagcaaaagcgcggatgcttgcgctatcatgggagctatcctggtggactggaattacggttggtgagcttgtcgaaccatgagtaattccggctctcgagcaacataaaactcatgcttcgactaacccttcgaaaagctcaggaaccgtctcagcaaccgttttgtgtattaaccggattat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02568:AA02569|AA02568:AA02569:sugar phosphotransferase component II B:hypothetical protein:->->:1788786..1789151 366 aattttctcctttttgaggttaattgattcagcaactgagatgcagtctatgacggaacagcagggggatcaatgcacaggtttgaaaactgtgatcaggacgacattttgagaaaggcttccgaaggtataagaacaggaatgacatctgatggttcaagcgtccacgcttggaccgacacaagcaaaagcgcggatgcttgcgctatcatgggagctatcctggtggactggaattacggttggtgagcttgtcgaaccatgagtaattccggctctcgagcaacataaaactcatgcttcgactaacccttcgaaaagctcaggaaccgtctcagcaaccgttttgtgtattaaccggattat Intra-Species Hit: Count: 1 Min: 1 Max: 366 Len: 366 Subject: aact_AA02568_AA02569|sugar phosphotransferase component II B:hypothetical protein|POSITIVE:POSITIVE|[1788786,1789151]|366 HSP 1 e-value: 0.0 bit: 726.0 Len: 366 Query Start:1 Query End:366 Subject Strand: POSITIVE Subject Start: 1 Subject End: 366 aattttctcctttttgaggttaattgattcagcaactgagatgcagtctatgacggaacagcagggggatcaatgcacaggtttgaaaactgtgatcaggacgacattttgagaaaggcttccgaaggtataagaacaggaatgacatctgatggttcaagcgtccacgcttggaccgacacaagcaaaagcgcggatgcttgcgctatcatgggagctatcctggtggactggaattacggttggtgagcttgtcgaaccatgagtaattccggctctcgagcaacataaaactcatgcttcgactaacccttcgaaaagctcaggaaccgtctcagcaaccgttttgtgtattaaccggattat aattttctcctttttgaggttaattgattcagcaactgagatgcagtctatgacggaacagcagggggatcaatgcacaggtttgaaaactgtgatcaggacgacattttgagaaaggcttccgaaggtataagaacaggaatgacatctgatggttcaagcgtccacgcttggaccgacacaagcaaaagcgcggatgcttgcgctatcatgggagctatcctggtggactggaattacggttggtgagcttgtcgaaccatgagtaattccggctctcgagcaacataaaactcatgcttcgactaacccttcgaaaagctcaggaaccgtctcagcaaccgttttgtgtattaaccggattat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUUUCUCCUUUUUGAGGUUAAUUGAUUCAGCAACUGAGAUGCAGUCUAUGACGGAACAGCAGGGGGAUCAAUGCACAGGUUUGAAAACUGUGAUCAGGACGACAUUUUGAGAAAGGCUUCCGAAGGUAUAAGAACAGGAAUGACAUCUGAUGGUUCAAGCGUCCACGCUUGGACCGACACAAGCAAAAGCGCGGAUGCUUGCGCUAUCAUGGGAGCUAUCCUGGUGGACUGGAAUUACGGUUGGUGAGCUUGUCGAACCAUGAGUAAUUCCGGCUCUCGAGCAACAUAAAACUCAUGCUUCGACUAACCCUUCGAAAAGCUCAGGAACCGUCUCAGCAACCGUUUUGUGUAUUAACCGGAUUAU .................(((((((..((..(((..((((((..((((((((..((((..(((.............(((((.........))))).((((((.....)))))).....)))))))............(((((..(...(((((((((.(((((((((.(((((................))))).))))))))).))))))).))...)..)))))))))))))......(((((..(((((((((((((.(((((((.......(((....)))........))))))).))))))............)))))))..))))))))))).....)))..))..)))))))....... (-113.95) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAUCCGGUUAAUACACAAAACGGUUGCUGAGACGGUUCCUGAGCUUUUCGAAGGGUUAGUCGAAGCAUGAGUUUUAUGUUGCUCGAGAGCCGGAAUUACUCAUGGUUCGACAAGCUCACCAACCGUAAUUCCAGUCCACCAGGAUAGCUCCCAUGAUAGCGCAAGCAUCCGCGCUUUUGCUUGUGUCGGUCCAAGCGUGGACGCUUGAACCAUCAGAUGUCAUUCCUGUUCUUAUACCUUCGGAAGCCUUUCUCAAAAUGUCGUCCUGAUCACAGUUUUCAAACCUGUGCAUUGAUCCCCCUGCUGUUCCGUCAUAGACUGCAUCUCAGUUGCUGAAUCAAUUAACCUCAAAAAGGAGAAAAUU ....((((((((((........((((.((((((((((((..((((((..((....))...((((((.(((((((........(((....))).......))))))).)))))).))))))..)))))......(((((...(((((..((.....((((.(..(((((.(((((((((..(((......)))..))))))))).)))))..).))))...))...)))))............(((((((.........((((..........(((((.........))))))))).........)))..)))).....)))))..))))))).)))).....))))))).......)))....... (-107.96) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 584 267 231 229 193 aact:AA01151|5end_porphobilinogen_synthase_(delta-aminolevulinic_acid_dehydratase)_788323..788553_POSITIVE 396 249 203 182 137 aact:AA00408|5end_SUN_protein_(FMU_protein)_272256..272486_POSITIVE 394 265 222 226 185 aact:AA02998|5end_conserved_hypothetical_protein_2093707..2093937_POSITIVE 385 198 153 124 73 aact:AA01432|5end_lysophospholipase_L2_960715..960945_POSITIVE 384 233 191 167 123 aact:AA01770|5end_hypothetical_protein_1193111..1193341_POSITIVE 381 210 164 175 115 aact:AA02450|5end_conserved_hypothetical_protein_1687701..1687931_POSITIVE 376 109 61 179 134 aact:AA01310|5end_conserved_hypothetical_protein_888726..888956_POSITIVE 372 207 161 70 3 aact:AA02100|5end_lipoprotein_1430074..1430304_POSITIVE 371 218 172 62 18 aact:AA00688|5end_curved_DNA-binding_protein_469165..469395_POSITIVE 370 288 241 122 75 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 362 249 204 62 1 aact:AA02713|5end_possible_prop_effector_homolog_1894984..1895214_POSITIVE 360 207 165 203 163 aact:AA00335|5end_PTS_fructose-specific_IIBC_component_226489..226719_POSITIVE 358 197 151 182 129 aact:AA02076|5end_conserved_hypothetical_protein_1409926..1410156_POSITIVE 356 240 192 52 1 aact:AA02425|5end_glutamate-1-semialdehyde_aminotransferase_1670301..1670531_POSITIVE 356 220 174 199 147 aact:AA01259|5end_protoporphyrinogen_oxidase_854354..854584_POSITIVE 355 276 236 222 175 aact:AA02442|5end_thioredoxin-family_protein_1685704..1685934_POSITIVE 353 208 162 74 16 aact:AA01872|5end_conserved_hypothetical_protein_1258804..1259034_POSITIVE 352 235 192 170 115 aact:AA01025|5end_hypothetical_protein_717522..717752_POSITIVE 352 210 161 217 171 aact:AA00788|5end_hypothetical_protein_540351..540581_POSITIVE 348 209 161 199 157 aact:AA01810|5end_electron_transport_complex_protein_1219250..1219480_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 396 353 311 95 56 aact:AA01953|3end_hypothetical_protein_1320349..1320499_POSITIVE 385 198 153 80 29 aact:AA01431|3end_conserved_hypothetical_protein_(possible_hydrolase)_960751..960901_POSITIVE 384 233 191 108 64 aact:AA01769|3end_hypothetical_protein_1193132..1193282_POSITIVE 376 109 61 105 60 aact:AA01308|3end_cyclic_AMP_receptor_protein_888732..888882_POSITIVE 370 288 241 117 70 aact:AA00323|3end_conserved_hypothetical_protein_(possible_SAM-dependent_methyltransferase)_220222..220372_POSITIVE 362 340 295 70 38 aact:AA01149|3end_glycyl-tRNA_synthetase_beta_subunit_788185..788335_POSITIVE 349 196 155 87 40 aact:AA02075|3end_conserved_hypothetical_protein_1409979..1410129_POSITIVE 347 208 161 140 94 aact:AA01137|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_A_781057..781207_POSITIVE 346 247 203 129 82 aact:AA00458|3end_nickel-binding_periplasmic_protein_320884..321034_POSITIVE 345 248 202 144 92 aact:AA00987|3end_tetracenomycin_polyketide_synthesis_O-methyltransferase_690586..690736_POSITIVE 344 204 161 133 87 aact:AA00744|3end_aldose_1-epimerase_(mutarotase)_511246..511396_POSITIVE 340 278 231 75 5 aact:AA00647|3end_protoporphyrinogen_oxidase_443314..443464_POSITIVE 337 196 161 56 15 aact:AA01751|3end_conserved_hypothetical_protein_1185392..1185542_POSITIVE 335 206 161 60 8 aact:AA02357|3end_bifunctional_protein:_aspartokinase_I;_homoserine_dehydrogenaseI_1617192..1617342_POSITIVE 335 220 184 146 108 aact:AA02006|3end_PEP--fructosephosphotransferase_system_repressor,_DeoR_family_1364993..1365143_POSITIVE 334 209 161 55 10 aact:AA00474|3end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_331724..331874_POSITIVE 333 200 156 65 21 aact:AA00601|3end_hypothetical_protein_409119..409269_POSITIVE 332 276 233 147 106 aact:AA01704|3end_tyrosine-specific_transport_protein_1157461..1157611_POSITIVE 332 247 203 149 110 aact:AA00407|3end_trk_system_potassium_uptake_protein_272309..272459_POSITIVE 325 210 168 42 2 aact:AA01866|3end_thiamin_ABC_transporter,_permease_protein_1254946..1255096_POSITIVE