Origin IGS: taagcgggattcataaaagtgcggtcagctaaaaaaacgtttttcaaactagccgcacttttgtttgttaagtatttagctataaattcatcttatctaacaatatcgtttgcattgtacgggttatttataccacgaagtagcaagttcatgattttggtcgggaacatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgatgttcccgaccaaaatcatgaacttgctacttcgtggtataaataacccgtacaatgcaaacgatattgttagataagatgaatttatagctaaatacttaacaaacaaaagtgcggctagtttgaaaaacgtttttttagctgaccgcacttttatgaatcccgctta Mask Tandem Repeat Region ================================================ taagcgggattcataaaagtgcggtcagctaaaaaaacgtttttcaaactagccgcacttttgtttgttaagtatttagctataaattcatcttatctaacaatatcgtttgcattgtacgggttatttataccacgaagtagcaagttcatgattttggtcgggaacatca Find is-nt database================================================ Query_seq: AA02562:AA02563|AA02562:AA02563:adenine specific methylase:transketolase C-terminal section:->->:1785207..1785378 172 taagcgggattcataaaagtgcggtcagctaaaaaaacgtttttcaaactagccgcacttttgtttgttaagtatttagctataaattcatcttatctaacaatatcgtttgcattgtacgggttatttataccacgaagtagcaagttcatgattttggtcgggaacatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02562:AA02563|AA02562:AA02563:adenine specific methylase:transketolase C-terminal section:->->:1785207..1785378 172 taagcgggattcataaaagtgcggtcagctaaaaaaacgtttttcaaactagccgcacttttgtttgttaagtatttagctataaattcatcttatctaacaatatcgtttgcattgtacgggttatttataccacgaagtagcaagttcatgattttggtcgggaacatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02562:AA02563|AA02562:AA02563:adenine specific methylase:transketolase C-terminal section:->->:1785207..1785378 172 taagcgggattcataaaagtgcggtcagctaaaaaaacgtttttcaaactagccgcacttttgtttgttaagtatttagctataaattcatcttatctaacaatatcgtttgcattgtacgggttatttataccacgaagtagcaagttcatgattttggtcgggaacatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagcgggattcataaaagtgcggtcagctaaaaaaacgtttttcaaactagccgcacttttgtttgttaagtatttagctataaattcatcttatctaacaatatcgtttgcattgtacgggttatttataccacgaagtagcaagttcatgattttggtcgggaacatca Predict ORF larger than 30AA ================================================ Protein_Len: 53 Strand: + Start: 13 End: 171 ............ M K V R S A K K T F F K L A A L L F V K Y L A I N S S Y L T I S F A L Y G L F I P R S S K F M I L V G N I . taagcgggattcataaaagtgcggtcagctaaaaaaacgtttttcaaactagccgcacttttgtttgttaagtatttagctataaattcatcttatctaacaatatcgtttgcattgtacgggttatttataccacgaagtagcaagttcatgattttggtcgggaacatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagcgggattcataaaagtgcggtcagctaaaaaaacgtttttcaaactagccgcacttttgtttgttaagtatttagctataaattcatcttatctaacaatatcgtttgcattgtacgggttatttataccacgaagtagcaagttcatgattttggtcgggaacatca Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 78 HP_score: -7.2 Tail_Score: -4.42386 Start: 19 End: 58 Full_Region: acttaacaaacaaaa gtgcggctagtttgaaaa acgt ttttttagctgaccgcac ttttatgaatcccgc ..................gtgcggctagtttgaaaaacgtttttttagctgaccgcac.................................................................................................................. Find igs database================================================ Query_seq: AA02562:AA02563|AA02562:AA02563:adenine specific methylase:transketolase C-terminal section:->->:1785207..1785378 172 taagcgggattcataaaagtgcggtcagctaaaaaaacgtttttcaaactagccgcacttttgtttgttaagtatttagctataaattcatcttatctaacaatatcgtttgcattgtacgggttatttataccacgaagtagcaagttcatgattttggtcgggaacatca Intra-Species Hit: Count: 1 Min: 1 Max: 172 Len: 172 Subject: aact_AA02562_AA02563|adenine specific methylase:transketolase C-terminal section|POSITIVE:POSITIVE|[1785207,1785378]|172 HSP 1 e-value: 2.0E-80 bit: 299.0 Len: 172 Query Start:1 Query End:172 Subject Strand: POSITIVE Subject Start: 1 Subject End: 172 taagcgggattcataaaagtgcggtcagctnnnnnnncgtttttcaaactagccgcacttttgtttgttaagtatttagctataaattcatcttatctaacaatatcgtttgcattgtacgggttatttataccacgaagtagcaagttcatgattttggtcgggaacatca taagcgggattcataaaagtgcggtcagctaaaaaaacgtttttcaaactagccgcacttttgtttgttaagtatttagctataaattcatcttatctaacaatatcgtttgcattgtacgggttatttataccacgaagtagcaagttcatgattttggtcgggaacatca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGCGGGAUUCAUAAAAGUGCGGUCAGCUAAAAAAACGUUUUUCAAACUAGCCGCACUUUUGUUUGUUAAGUAUUUAGCUAUAAAUUCAUCUUAUCUAACAAUAUCGUUUGCAUUGUACGGGUUAUUUAUACCACGAAGUAGCAAGUUCAUGAUUUUGGUCGGGAACAUCA ...((..(((..((((((((((((..((..(((((....)))))....))..))))))))))))..)))..)).....(((((................(((((.........))))).(((((.......))).))..)))))..((((.((((....)))).)))).... (-38.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUGUUCCCGACCAAAAUCAUGAACUUGCUACUUCGUGGUAUAAAUAACCCGUACAAUGCAAACGAUAUUGUUAGAUAAGAUGAAUUUAUAGCUAAAUACUUAACAAACAAAAGUGCGGCUAGUUUGAAAAACGUUUUUUUAGCUGACCGCACUUUUAUGAAUCCCGCUUA ..................(((((((.((((.....((.(((.......)))))......))))......(((((.(.((((....(((((....))))).)))).).))))).(((((((.(((((.(((((....))))).))))).)))))))))))))).......... (-32.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 486 60 13 168 121 aact:AA00721|5end_probable_beta-N-acetylhexosaminidase_(lacto-N-biosidase);_dispersin_B_494739..494969_POSITIVE 486 60 13 56 9 aact:AA00719|5end_hypothetical_protein_494627..494857_POSITIVE 420 60 13 158 111 aact:AA00766|5end_heat_shock_protein_B25.3_(HSP-70_cofactor)_527384..527614_POSITIVE 410 60 15 150 105 aact:AA01648|5end_alpha-amylase_protein;_1,4-alpha-D-glucan_glucanohydrolase_1115170..1115400_POSITIVE 410 60 15 49 4 aact:AA01646|5end_hypothetical_protein_1115069..1115299_POSITIVE 410 60 15 170 125 aact:AA01117|5end_signal_recognition_particle_protein_54_(GTP-binding_export_factor)_767919..768149_POSITIVE 397 60 12 46 3 aact:AA01798|5end_hypothetical_protein_1212070..1212300_POSITIVE 391 60 15 219 174 aact:AA02778|5end_hypothetical_protein_1942585..1942815_POSITIVE 391 68 21 213 169 aact:AA00912|5end_short-chain_dehydrogenase_627200..627430_POSITIVE 391 68 21 101 57 aact:AA00910|5end_hypothetical_protein_627088..627318_POSITIVE 380 60 16 153 114 aact:AA02113|5end_conserved_hypothetical_protein_1442183..1442413_POSITIVE 377 60 15 157 112 aact:AA02700|5end_succinyl-CoA_synthase,_alpha_subunit_1883256..1883486_POSITIVE 359 60 15 150 105 aact:AA00386|5end_aspartate--ammonia_ligase_(asparagine_synthetase_A)_261150..261380_POSITIVE 357 60 16 162 118 aact:AA01967|5end_hypothetical_protein_1331511..1331741_POSITIVE 354 49 4 231 189 aact:AA02168|5end_possible_protease;_heat_shock_protein_1483529..1483759_POSITIVE 354 60 15 164 119 aact:AA01981|5end_4-alpha-glucanotransferase_1337202..1337432_POSITIVE 353 60 15 149 104 aact:AA02888|5end_ssrA-binding_protein,_small_protein_B_2024628..2024858_POSITIVE 340 60 15 155 116 aact:AA01085|5end_mutator_mutT_protein_(7,8-dihydro-8-oxoguanine-triphosphatase)_(8-oxo-dGTPase)_(dGTP_pyrophosphohydrolase)_750970..751200_POSITIVE 339 60 15 167 111 aact:AA02597|5end_ribonuclease_D_1807124..1807354_POSITIVE 338 60 13 134 92 aact:AA00091|5end_short-patch_reverse_transcription_protein_67741..67971_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 486 60 13 72 25 aact:AA00718|3end_ABC-related_ATP-binding_protein;_MRP_protein_494723..494873_POSITIVE 465 60 16 74 30 aact:AA00629|3end_inosine_5'_monophosphate_dehydrogenase_(IMP_dehydrogenase)_433469..433619_POSITIVE 410 60 15 84 39 aact:AA01645|3end_ferric_transport_ABC-related_ATP-binding_protein_1115184..1115334_POSITIVE 397 60 12 64 21 aact:AA01796|3end_branched-chain_amino_acid_aminotransferase_1212168..1212318_POSITIVE 391 60 15 81 36 aact:AA02777|3end_tRNA-guanine_transglycosylase_1942527..1942677_POSITIVE 391 68 21 116 72 aact:AA00909|3end_molybdopterin-guanine_dinucleotide_biosynthesis_protein_B_627183..627333_POSITIVE 380 60 16 74 35 aact:AA02112|3end_cell_division_protein_1442184..1442334_POSITIVE 376 60 13 74 27 aact:AA02562|3end_adenine_specific_methylase_1785146..1785296_POSITIVE 364 60 15 85 45 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 359 60 15 88 43 aact:AA00384|3end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_261168..261318_POSITIVE 357 60 16 85 41 aact:AA01966|3end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_1331514..1331664_POSITIVE 354 49 4 96 54 aact:AA02167|3end_hypothetical_protein_1483474..1483624_POSITIVE 354 60 15 139 94 aact:AA01978|3end_hypothetical_protein_1337257..1337407_POSITIVE 353 60 15 75 30 aact:AA02886|3end_OBG-related_GTP-binding_protein_2024634..2024784_POSITIVE 350 60 13 77 33 aact:AA00218|3end_argininosuccinate_lyase_152211..152361_POSITIVE 340 60 15 75 36 aact:AA01083|3end_preprotein_translocase_SecA_subunit_750970..751120_POSITIVE 339 60 15 73 17 aact:AA02595|3end_cytochrome_c_peroxidase_1807110..1807260_POSITIVE 338 60 13 62 20 aact:AA00089|3end_S-adenosylmethionine_synthase;_methionine_adenosyltransferase_67749..67899_POSITIVE 338 60 14 56 15 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 336 60 13 148 105 aact:AA00697|3end_hypothetical_protein_477174..477324_POSITIVE