Origin IGS: tttcgtctcctaaaatcgaataaaaacaaaaaaccagccattaaatacaattttctatttgcatttccgcgaatttggaatagcatacagccaacgctttatgctataaagtgtcacttaattttactcaaaacaggagcaaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atttgctcctgttttgagtaaaattaagtgacactttatagcataaagcgttggctgtatgctattccaaattcgcggaaatgcaaatagaaaattgtatttaatggctggttttttgtttttattcgattttaggagacgaaa Mask Tandem Repeat Region ================================================ tttcgtctcctaaaatcgaataaaaacaaaaaaccagccattaaatacaattttctatttgcatttccgcgaatttggaatagcatacagccaacgctttatgctataaagtgtcacttaattttactcaaaacaggagcaaat Find is-nt database================================================ Query_seq: AA02553:AA02554|AA02553:AA02554:acylphosphatase:conserved hypothetical protein:->->:1779731..1779874 144 tttcgtctcctaaaatcgaataaaaacaaaaaaccagccattaaatacaattttctatttgcatttccgcgaatttggaatagcatacagccaacgctttatgctataaagtgtcacttaattttactcaaaacaggagcaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02553:AA02554|AA02553:AA02554:acylphosphatase:conserved hypothetical protein:->->:1779731..1779874 144 tttcgtctcctaaaatcgaataaaaacaaaaaaccagccattaaatacaattttctatttgcatttccgcgaatttggaatagcatacagccaacgctttatgctataaagtgtcacttaattttactcaaaacaggagcaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02553:AA02554|AA02553:AA02554:acylphosphatase:conserved hypothetical protein:->->:1779731..1779874 144 tttcgtctcctaaaatcgaataaaaacaaaaaaccagccattaaatacaattttctatttgcatttccgcgaatttggaatagcatacagccaacgctttatgctataaagtgtcacttaattttactcaaaacaggagcaaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttcgtctcctaaaatcgaataaaaacaaaaaaccagccattaaatacaattttctatttgcatttccgcgaatttggaatagcatacagccaacgctttatgctataaagtgtcacttaattttactcaaaacaggagcaaat Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 40 End: 144 ....................................... M K Y N F L F A F P R I W N S I Q P T L Y A I K C H L I L L K T G A N tttcgtctcctaaaatcgaataaaaacaaaaaaccagccattaaatacaattttctatttgcatttccgcgaatttggaatagcatacagccaacgctttatgctataaagtgtcacttaattttactcaaaacaggagcaaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 57 End: 86 ........................................................ATTTGCATTTCCGCGAATTTGGAATAGCAT.......................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttcgtctcctaaaatcgaataaaaacaaaaaaccagccattaaatacaattttctatttgcatttccgcgaatttggaatagcatacagccaacgctttatgctataaagtgtcacttaattttactcaaaacaggagcaaat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02553:AA02554|AA02553:AA02554:acylphosphatase:conserved hypothetical protein:->->:1779731..1779874 144 tttcgtctcctaaaatcgaataaaaacaaaaaaccagccattaaatacaattttctatttgcatttccgcgaatttggaatagcatacagccaacgctttatgctataaagtgtcacttaattttactcaaaacaggagcaaat Intra-Species Hit: Count: 1 Min: 34 Max: 144 Len: 111 Subject: aact_AA02553_AA02554|acylphosphatase:conserved hypothetical protein|POSITIVE:POSITIVE|[1779731,1779874]|144 HSP 1 e-value: 1.0E-56 bit: 220.0 Len: 111 Query Start:34 Query End:144 Subject Strand: POSITIVE Subject Start: 34 Subject End: 144 .................................ccagccattaaatacaattttctatttgcatttccgcgaatttggaatagcatacagccaacgctttatgctataaagtgtcacttaattttactcaaaacaggagcaaat .................................ccagccattaaatacaattttctatttgcatttccgcgaatttggaatagcatacagccaacgctttatgctataaagtgtcacttaattttactcaaaacaggagcaaat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CCAGCCAUUAAAUACAAUUUUCUAUUUGCAUUUCCGCGAAUUUGGAAUAGCAUACAGCCAACGCUUUAUGCUAUAAAGUGUCACUUAAUUUUACUCAAAACAGGAGCAAAU .......................((((((...(((.....(((((.((((((((.(((....))).)))))))).(((.....)))........)))))...))))))))) (-19.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUGCUCCUGUUUUGAGUAAAAUUAAGUGACACUUUAUAGCAUAAAGCGUUGGCUGUAUGCUAUUCCAAAUUCGCGGAAAUGCAAAUAGAAAAUUGUAUUUAAUGGCUGG .(((((((.......))))))).....((((......((((((((.(((....))).)))))))).......))))..((((((((........))))))))......... (-26.42) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 308 80 34 148 105 aact:AA02077|5end_probable_outer_membrane_protein_1411294..1411524_POSITIVE 279 66 24 134 94 aact:AA00240|5end_hypothetical_protein_171390..171620_POSITIVE 266 72 34 70 24 aact:AA00052|5end_cytochrome_c-type_biogenesis_protein_precursor_40842..41072_POSITIVE 265 80 34 65 12 aact:AA01666|5end_penicillin-binding_protein_4;_D-alanyl-D-alanine_carboxypeptidase;_D-alanyl-D-alanine-endopeptidase_1128231..1128461_POSITIVE 256 63 22 215 168 aact:AA00549|5end_ferredoxin-type_protein_375563..375793_POSITIVE 255 76 31 94 59 aact:AA02151|5end_ferric_enterobactin_receptor;_TonB-dependent_outer_membrane_siderophore_receptor_protein_1470856..1471086_POSITIVE 254 46 4 219 176 aact:AA02265|5end_exodeoxyribonuclease_V,_alpha_chain_1539701..1539931_POSITIVE 253 77 34 201 163 aact:AA01117|5end_signal_recognition_particle_protein_54_(GTP-binding_export_factor)_767919..768149_POSITIVE 252 60 12 214 160 aact:AA01194|5end_acyl_carrier_protein_819595..819825_POSITIVE 251 55 23 75 34 aact:AA00735|5end_citrate_lyase,_beta_chain_502674..502904_POSITIVE 247 64 22 46 4 aact:AA02885|5end_conserved_hypothetical_protein_2022428..2022658_POSITIVE 246 71 31 194 158 aact:AA02025|5end_hypothetical_protein_1378667..1378897_POSITIVE 246 63 30 73 43 aact:AA00682|5end_S-adenosylmethionine-dependent_methyltransferase_466322..466552_POSITIVE 245 77 36 231 181 aact:AA00809|5end_possible_oxidoreductase_553211..553441_POSITIVE 243 80 34 165 126 aact:AA01355|5end_large_subunit_pseudouridylate_synthase_D_917228..917458_POSITIVE 240 74 34 50 19 aact:AA01743|5end_lysyl-tRNA_synthetase_1177808..1178038_POSITIVE 240 72 33 226 187 aact:AA00047|5end_formate_dependent_nitrite_reductase,_cytochrome_c552_36753..36983_POSITIVE 238 75 35 92 47 aact:AA01360|5end_XAA-pro_aminopeptidase_(X-pro_aminopeptidase)_921047..921277_POSITIVE 237 66 24 163 110 aact:AA01434|5end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_961631..961861_POSITIVE 237 75 35 77 41 aact:AA00578|5end_DNA_topoisomerase_394576..394806_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 322 81 42 132 88 aact:AA00807|3end_fumarate_reductase_13_kD_hydrophobic_protein_553188..553338_POSITIVE 279 66 24 82 42 aact:AA00239|3end_hypothetical_protein_171418..171568_POSITIVE 266 72 34 67 21 aact:AA00050|3end_cytochrome_c-type_biogenesis_protein_precursor_(reductase)_40919..41069_POSITIVE 255 76 31 143 108 aact:AA02150|3end_conserved_hypothetical_protein_1470985..1471135_POSITIVE 253 77 34 42 4 aact:AA01116|3end_hypothetical_protein_767840..767990_POSITIVE 253 62 22 123 79 aact:AA01009|3end_universal_stress_protein_A_704945..705095_POSITIVE 251 55 23 75 34 aact:AA00734|3end_citrate_lyase,_gamma_chain_(acyl_carrier_protein)_502754..502904_POSITIVE 240 72 33 149 110 aact:AA00046|3end_formate_dependent_nitrite_reductase,_cytochrome-c_type_protein_36756..36906_POSITIVE 238 75 35 74 29 aact:AA01359|3end_conserved_hypothetical_protein_921109..921259_POSITIVE 237 75 35 69 33 aact:AA00576|3end_conserved_hypothetical_protein_(possible_translation_factor)_394648..394798_POSITIVE 236 72 33 137 98 aact:AA01439|3end_probable_ferredoxin_(fdx-2)_966498..966648_POSITIVE 235 79 38 116 64 aact:AA00765|3end_hypothetical_protein_528132..528282_POSITIVE 232 70 28 60 20 aact:AA01539|3end_UDP-N-acetylglucosamine_pyrophosphorylase_(bifunctional_enzyme)_1034187..1034337_POSITIVE 229 59 15 118 81 aact:AA02684|3end_hydrogenase_4_membrane_subunit_1871612..1871762_POSITIVE 229 59 28 64 31 aact:AA01956|3end_3-deoxy-manno-octulosonate_cytidylyltransferase_1323286..1323436_POSITIVE 227 73 34 116 71 aact:AA01335|3end_primosomal_protein_N'_904109..904259_POSITIVE 227 68 21 104 56 aact:AA00894|3end_selenium_transferase_(Cysteinyl-tRNA(Sec)_selenium_transferase)_621350..621500_POSITIVE 227 67 25 83 42 aact:AA00474|3end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_331724..331874_POSITIVE 226 80 36 151 102 aact:AA02922|3end_gamma-glutamylphosphate_reductase_2047441..2047591_POSITIVE 225 67 24 132 94 aact:AA01011|3end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_707769..707919_POSITIVE