Origin IGS: ggttttatctctttttcgtgtcagtgattaaggataacttaatcactgctttcttattgaataggcagttacttaaaaattttaaataactgaaattatccacccaatggcttgttaccttcttaaactattctca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgagaatagtttaagaaggtaacaagccattgggtggataatttcagttatttaaaatttttaagtaactgcctattcaataagaaagcagtgattaagttatccttaatcactgacacgaaaaagagataaaacc Mask Tandem Repeat Region ================================================ ggttttatctctttttcgtgtcagtgattaaggataacttaatcactgctttcttattgaataggcagttacttaaaaattttaaataactgaaattatccacccaatggcttgttaccttcttaaactattctca Find is-nt database================================================ Query_seq: AA02541:AA02543|AA02541:AA02543:conserved hypothetical protein:conserved hypothetical protein:->->:1772705..1772840 136 ggttttatctctttttcgtgtcagtgattaaggataacttaatcactgctttcttattgaataggcagttacttaaaaattttaaataactgaaattatccacccaatggcttgttaccttcttaaactattctca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02541:AA02543|AA02541:AA02543:conserved hypothetical protein:conserved hypothetical protein:->->:1772705..1772840 136 ggttttatctctttttcgtgtcagtgattaaggataacttaatcactgctttcttattgaataggcagttacttaaaaattttaaataactgaaattatccacccaatggcttgttaccttcttaaactattctca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02541:AA02543|AA02541:AA02543:conserved hypothetical protein:conserved hypothetical protein:->->:1772705..1772840 136 ggttttatctctttttcgtgtcagtgattaaggataacttaatcactgctttcttattgaataggcagttacttaaaaattttaaataactgaaattatccacccaatggcttgttaccttcttaaactattctca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ggttttatctctttttcgtgtcagtgattaaggataacttaatcactgctttcttattgaataggcagttacttaaaaattttaaataactgaaattatccacccaatggcttgttaccttcttaaactattctca Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: - Start: 8 End: 103 ....... R K K T D T I L S L K I V A K K N F L C N S L F K L Y S F N D M ................................. ggttttatctctttttcgtgtcagtgattaaggataacttaatcactgctttcttattgaataggcagttacttaaaaattttaaataactgaaattatccacccaatggcttgttaccttcttaaactattctca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ggttttatctctttttcgtgtcagtgattaaggataacttaatcactgctttcttattgaataggcagttacttaaaaattttaaataactgaaattatccacccaatggcttgttaccttcttaaactattctca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02541:AA02543|AA02541:AA02543:conserved hypothetical protein:conserved hypothetical protein:->->:1772705..1772840 136 ggttttatctctttttcgtgtcagtgattaaggataacttaatcactgctttcttattgaataggcagttacttaaaaattttaaataactgaaattatccacccaatggcttgttaccttcttaaactattctca Intra-Species Hit: Count: 1 Min: 1 Max: 136 Len: 136 Subject: aact_AA02541_AA02543|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1772705,1772840]|136 HSP 1 e-value: 1.0E-71 bit: 270.0 Len: 136 Query Start:1 Query End:136 Subject Strand: POSITIVE Subject Start: 1 Subject End: 136 ggttttatctctttttcgtgtcagtgattaaggataacttaatcactgctttcttattgaataggcagttacttaaaaattttaaataactgaaattatccacccaatggcttgttaccttcttaaactattctca ggttttatctctttttcgtgtcagtgattaaggataacttaatcactgctttcttattgaataggcagttacttaaaaattttaaataactgaaattatccacccaatggcttgttaccttcttaaactattctca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUUUUAUCUCUUUUUCGUGUCAGUGAUUAAGGAUAACUUAAUCACUGCUUUCUUAUUGAAUAGGCAGUUACUUAAAAAUUUUAAAUAACUGAAAUUAUCCACCCAAUGGCUUGUUACCUUCUUAAACUAUUCUCA ...................(.(((((((((((.....)))))))))))).........((((((.((((((.((((.....)))).)))))).......(((.....))).................))))))... (-27.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAGAAUAGUUUAAGAAGGUAACAAGCCAUUGGGUGGAUAAUUUCAGUUAUUUAAAAUUUUUAAGUAACUGCCUAUUCAAUAAGAAAGCAGUGAUUAAGUUAUCCUUAAUCACUGACACGAAAAAGAGAUAAAACC ...((((((........(((.....)))................(((((((((((.....))))))))))).)))))).........((((((((((((.....))))))))))).)................... (-31.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 249 119 79 195 151 aact:AA01825|5end_iron(III)_ABC_transporter,_permease_protein_1228475..1228705_POSITIVE 236 68 23 55 13 aact:AA00660|5end_hypothetical_protein_453031..453261_POSITIVE 229 59 11 198 147 aact:AA02888|5end_ssrA-binding_protein,_small_protein_B_2024628..2024858_POSITIVE 226 43 3 127 86 aact:AA02388|5end_alpha-glucosidase_1636138..1636368_POSITIVE 225 34 1 51 13 aact:AA00410|5end_peptide_deformylase_(PDF)_(Polypeptide_deformylase)_274633..274863_POSITIVE 220 49 1 55 10 aact:AA02430|5end_conserved_hypothetical_protein_1675251..1675481_POSITIVE 216 52 17 171 144 aact:AA00864|5end_conserved_hypothetical_protein_598605..598835_POSITIVE 208 114 90 142 113 aact:AA01472|5end_hypothetical_protein_988896..989126_POSITIVE 205 119 96 23 3 aact:AA01402|5end_beta_1-4_glucosyltransferase_947544..947774_POSITIVE 202 124 85 166 127 aact:AA00882|5end_hypothetical_protein_613452..613682_POSITIVE 201 122 101 139 116 aact:AA02037|5end_hypothetical_protein_1386832..1387062_POSITIVE 200 123 89 74 31 aact:AA02902|5end_aerobic_respiration_control_protein_2031171..2031401_POSITIVE 200 130 95 157 123 aact:AA02196|5end_oxaloacetate_decarboxylase_beta_chain_1496308..1496538_POSITIVE 200 70 21 204 156 aact:AA01624|5end_glucose-6-phosphate_isomerase_1098175..1098405_POSITIVE 199 50 5 208 169 aact:AA01890|5end_cell-cycle_protein_1274544..1274774_POSITIVE 198 63 21 210 160 aact:AA01471|5end_type_I_restriction_enzyme_M_(modification_chain)_987321..987551_POSITIVE 198 124 92 153 119 aact:AA01231|5end_30S_ribosomal_protein_S14_840103..840333_POSITIVE 196 49 2 181 133 aact:AA01337|5end_cell_division_protein_N_904235..904465_POSITIVE 195 69 21 58 15 aact:AA02593|5end_conserved_hypothetical_protein_(exported_protein)_1804058..1804288_POSITIVE 195 121 90 187 152 aact:AA00982|5end_aldo-keto_reductase_686526..686756_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 237 48 22 69 43 aact:AA02541|3end_conserved_hypothetical_protein_1772644..1772794_POSITIVE 229 59 11 124 73 aact:AA02886|3end_OBG-related_GTP-binding_protein_2024634..2024784_POSITIVE 229 130 89 80 43 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 228 49 1 134 83 aact:AA01857|3end_glutamate-ammonia-ligase_adenylyltransferase_1250357..1250507_POSITIVE 226 43 3 100 59 aact:AA02387|3end_integral_membrane_transport_protein_1636191..1636341_POSITIVE 224 59 15 110 56 aact:AA02602|3end_conserved_hypothetical_protein_1814302..1814452_POSITIVE 220 120 105 148 133 aact:AA01070|3end_DNA_repair_protein_741683..741833_POSITIVE 219 46 8 60 28 aact:AA01170|3end_hypothetical_protein_802639..802789_POSITIVE 206 59 14 38 1 aact:AA00989|3end_hypothetical_protein_692644..692794_POSITIVE 203 119 74 125 79 aact:AA00422|3end_LapB_membrane_protein_286480..286630_POSITIVE 202 124 85 119 80 aact:AA00881|3end_hypothetical_protein_613485..613635_POSITIVE 201 122 101 132 109 aact:AA02035|3end_translation_initiation_factor_IF-3_1386905..1387055_POSITIVE 200 130 95 143 109 aact:AA02195|3end_oxaloacetate_decarboxylase_alpha_chain_1496374..1496524_POSITIVE 198 124 92 137 103 aact:AA01230|3end_50S_ribosomal_protein_L5_840167..840317_POSITIVE 193 58 16 130 89 aact:AA02481|3end_conserved_hypothetical_protein_1720103..1720253_POSITIVE 193 74 32 62 20 aact:AA01972|3end_phosphotransacetylase_1334431..1334581_POSITIVE 192 68 23 76 24 aact:AA01609|3end_p-aminobenzoate_synthase_component_I_homolog_1088822..1088972_POSITIVE 189 114 97 58 40 aact:AA01728|3end_conserved_hypothetical_protein(related_to_HipA_protein)_1168665..1168815_POSITIVE 189 73 39 75 51 aact:AA01354|3end_conserved_hypothetical_protein_917321..917471_POSITIVE 189 34 1 82 52 aact:AA00439|3end_conserved_hypothetical_protein_(possible_recombinase)_305438..305588_POSITIVE