Origin IGS: cataaaccccataaaaatcgattcaataaattaagcgccagattatagcaaaaagccttgttattttaaatgcgcccatgcgccgtaaaaacacgaggaaaaaccaccgcacttaatctgtcacttcactaaaacatgaacccaattaattaactaaaaagctaccctggatatttcgctacggaaggtagtcaaagttacaaaattttgtatcaaatattttataaatttattctttacattaaaccaaacaaaaatataatgcgaatcgtttttatctatggccagaatctgaggaaatact .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agtatttcctcagattctggccatagataaaaacgattcgcattatatttttgtttggtttaatgtaaagaataaatttataaaatatttgatacaaaattttgtaactttgactaccttccgtagcgaaatatccagggtagctttttagttaattaattgggttcatgttttagtgaagtgacagattaagtgcggtggtttttcctcgtgtttttacggcgcatgggcgcatttaaaataacaaggctttttgctataatctggcgcttaatttattgaatcgatttttatggggtttatg Mask Tandem Repeat Region ================================================ cataaaccccataaaaatcgattcaataaattaagcgccagattatagcaaaaagccttgttattttaaatgcgcccatgcgccgtaaaaacacgaggaaaaaccaccgcacttaatctgtcacttcactaaaacatgaacccaattaattaactaaaaagctaccctggatatttcgctacggaaggtagtcaaagttacaaaattttgtatcaaatattttataaatttattctttacattaaaccaaacaaaaatataatgcgaatcgtttttatctatggccagaatctgaggaaatact Find is-nt database================================================ Query_seq: AA02531:AA02532|AA02531:AA02532:conserved hypothetical protein:conserved hypothetical protein:->->:1759402..1759705 304 cataaaccccataaaaatcgattcaataaattaagcgccagattatagcaaaaagccttgttattttaaatgcgcccatgcgccgtaaaaacacgaggaaaaaccaccgcacttaatctgtcacttcactaaaacatgaacccaattaattaactaaaaagctaccctggatatttcgctacggaaggtagtcaaagttacaaaattttgtatcaaatattttataaatttattctttacattaaaccaaacaaaaatataatgcgaatcgtttttatctatggccagaatctgaggaaatact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02531:AA02532|AA02531:AA02532:conserved hypothetical protein:conserved hypothetical protein:->->:1759402..1759705 304 cataaaccccataaaaatcgattcaataaattaagcgccagattatagcaaaaagccttgttattttaaatgcgcccatgcgccgtaaaaacacgaggaaaaaccaccgcacttaatctgtcacttcactaaaacatgaacccaattaattaactaaaaagctaccctggatatttcgctacggaaggtagtcaaagttacaaaattttgtatcaaatattttataaatttattctttacattaaaccaaacaaaaatataatgcgaatcgtttttatctatggccagaatctgaggaaatact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02531:AA02532|AA02531:AA02532:conserved hypothetical protein:conserved hypothetical protein:->->:1759402..1759705 304 cataaaccccataaaaatcgattcaataaattaagcgccagattatagcaaaaagccttgttattttaaatgcgcccatgcgccgtaaaaacacgaggaaaaaccaccgcacttaatctgtcacttcactaaaacatgaacccaattaattaactaaaaagctaccctggatatttcgctacggaaggtagtcaaagttacaaaattttgtatcaaatattttataaatttattctttacattaaaccaaacaaaaatataatgcgaatcgtttttatctatggccagaatctgaggaaatact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cataaaccccataaaaatcgattcaataaattaagcgccagattatagcaaaaagccttgttattttaaatgcgcccatgcgccgtaaaaacacgaggaaaaaccaccgcacttaatctgtcacttcactaaaacatgaacccaattaattaactaaaaagctaccctggatatttcgctacggaaggtagtcaaagttacaaaattttgtatcaaatattttataaatttattctttacattaaaccaaacaaaaatataatgcgaatcgtttttatctatggccagaatctgaggaaatact Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 167 End: 259 ...................................................................................................................................................................... M D I S L R K V V K V T K F C I K Y F I N L F F T L N Q T K I ............................................. Protein_Len: 33 Strand: + Start: 205 End: 303 ............................................................................................................................................................................................................ M L Y Q I F Y K F I L Y I K P N K N I M R I V F I Y G Q N L R K Y . Protein_Len: 45 Strand: - Start: 157 End: 291 ............................................................................................................................................................ F A V R S I E S R F T T L T V F N Q I L Y K I F K N K V N F W V F I Y H S D N K D I A L M ............. cataaaccccataaaaatcgattcaataaattaagcgccagattatagcaaaaagccttgttattttaaatgcgcccatgcgccgtaaaaacacgaggaaaaaccaccgcacttaatctgtcacttcactaaaacatgaacccaattaattaactaaaaagctaccctggatatttcgctacggaaggtagtcaaagttacaaaattttgtatcaaatattttataaatttattctttacattaaaccaaacaaaaatataatgcgaatcgtttttatctatggccagaatctgaggaaatact Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 87 Start: 42 End: 71 .........................................ATTTAAAATAACAAGGCTTTTTGCTATAAT......................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cataaaccccataaaaatcgattcaataaattaagcgccagattatagcaaaaagccttgttattttaaatgcgcccatgcgccgtaaaaacacgaggaaaaaccaccgcacttaatctgtcacttcactaaaacatgaacccaattaattaactaaaaagctaccctggatatttcgctacggaaggtagtcaaagttacaaaattttgtatcaaatattttataaatttattctttacattaaaccaaacaaaaatataatgcgaatcgtttttatctatggccagaatctgaggaaatact Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02531:AA02532|AA02531:AA02532:conserved hypothetical protein:conserved hypothetical protein:->->:1759402..1759705 304 cataaaccccataaaaatcgattcaataaattaagcgccagattatagcaaaaagccttgttattttaaatgcgcccatgcgccgtaaaaacacgaggaaaaaccaccgcacttaatctgtcacttcactaaaacatgaacccaattaattaactaaaaagctaccctggatatttcgctacggaaggtagtcaaagttacaaaattttgtatcaaatattttataaatttattctttacattaaaccaaacaaaaatataatgcgaatcgtttttatctatggccagaatctgaggaaatact Intra-Species Hit: Count: 1 Min: 1 Max: 304 Len: 304 Subject: aact_AA02531_AA02532|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1759402,1759705]|304 HSP 1 e-value: 1.0E-171 bit: 603.0 Len: 304 Query Start:1 Query End:304 Subject Strand: POSITIVE Subject Start: 1 Subject End: 304 cataaaccccataaaaatcgattcaataaattaagcgccagattatagcaaaaagccttgttattttaaatgcgcccatgcgccgtaaaaacacgaggaaaaaccaccgcacttaatctgtcacttcactaaaacatgaacccaattaattaactaaaaagctaccctggatatttcgctacggaaggtagtcaaagttacaaaattttgtatcaaatattttataaatttattctttacattaaaccaaacaaaaatataatgcgaatcgtttttatctatggccagaatctgaggaaatact cataaaccccataaaaatcgattcaataaattaagcgccagattatagcaaaaagccttgttattttaaatgcgcccatgcgccgtaaaaacacgaggaaaaaccaccgcacttaatctgtcacttcactaaaacatgaacccaattaattaactaaaaagctaccctggatatttcgctacggaaggtagtcaaagttacaaaattttgtatcaaatattttataaatttattctttacattaaaccaaacaaaaatataatgcgaatcgtttttatctatggccagaatctgaggaaatact Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAUAAACCCCAUAAAAAUCGAUUCAAUAAAUUAAGCGCCAGAUUAUAGCAAAAAGCCUUGUUAUUUUAAAUGCGCCCAUGCGCCGUAAAAACACGAGGAAAAACCACCGCACUUAAUCUGUCACUUCACUAAAACAUGAACCCAAUUAAUUAACUAAAAAGCUACCCUGGAUAUUUCGCUACGGAAGGUAGUCAAAGUUACAAAAUUUUGUAUCAAAUAUUUUAUAAAUUUAUUCUUUACAUUAAACCAAACAAAAAUAUAAUGCGAAUCGUUUUUAUCUAUGGCCAGAAUCUGAGGAAAUACU ........(((((((((.((((((.((((((((((.(.(((((((..((......((((((..(((((...((((....))))..)))))..))))))..........))...))))))).).)))..........(((((..((((...(((((.....(((((((((............)))..))))))...)))))...))))..)).)))...........)))))))......(((((...............))))).)))))).))))...))))).................... (-47.45) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUAUUUCCUCAGAUUCUGGCCAUAGAUAAAAACGAUUCGCAUUAUAUUUUUGUUUGGUUUAAUGUAAAGAAUAAAUUUAUAAAAUAUUUGAUACAAAAUUUUGUAACUUUGACUACCUUCCGUAGCGAAAUAUCCAGGGUAGCUUUUUAGUUAAUUAAUUGGGUUCAUGUUUUAGUGAAGUGACAGAUUAAGUGCGGUGGUUUUUCCUCGUGUUUUUACGGCGCAUGGGCGCAUUUAAAAUAACAAGGCUUUUUGCUAUAAUCUGGCGCUUAAUUUAUUGAAUCGAUUUUUAUGGGGUUUAUG .(((...(((((...((((....))))((((((((((((((((((.(((.......))).))))))....((((((((..(((((((..(((.((..((((...(((((..(((((((((..(((......)))..))))))).))...)))))...))))..))))))))))))...(((((.(((((((...((((.((((((......((((((....((((....))))....))))))..))))))..))))..))))))).))))))))))))))))))).)))))))))))..))). (-66.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 457 117 74 61 5 aact:AA01937|5end_sugar_fermentation_stimulation_protein_1308478..1308708_POSITIVE 419 120 72 186 121 aact:AA02668|5end_gluconate_permease_1856758..1856988_POSITIVE 417 128 82 90 38 aact:AA00750|5end_periplasmic_D-galactose-binding_ABC_transport_protein_514904..515134_POSITIVE 410 114 71 93 48 aact:AA01641|5end_hypothetical_protein_1110819..1111049_POSITIVE 410 126 81 201 158 aact:AA00268|5end_acyl_carrier_protein_(ACP)_188104..188334_POSITIVE 410 126 81 50 7 aact:AA00267|5end_hypothetical_protein_187953..188183_POSITIVE 408 120 81 221 175 aact:AA00515|5end_hypothetical_protein_355730..355960_POSITIVE 408 120 81 100 54 aact:AA00514|5end_hypothetical_protein_355609..355839_POSITIVE 405 114 72 176 138 aact:AA02144|5end_iron_(III)_ABC_transporter,_permease_protein_1467583..1467813_POSITIVE 394 129 83 212 165 aact:AA02944|5end_30S_ribosomal_protein_S20_2058688..2058918_POSITIVE 394 129 83 59 12 aact:AA02941|5end_hypothetical_protein_2058535..2058765_POSITIVE 387 114 73 120 74 aact:AA01390|5end_LexA_repressor_937059..937289_POSITIVE 386 120 81 80 42 aact:AA02123|5end_hypothetical_protein_1448244..1448474_POSITIVE 377 120 73 125 73 aact:AA01187|5end_conserved_hypothetical_protein_813331..813561_POSITIVE 364 129 82 149 104 aact:AA00409|5end_methionyl-tRNA_formyltransferase_273611..273841_POSITIVE 362 114 80 131 94 aact:AA00075|5end_conserved_hypothetical_protein_54963..55193_POSITIVE 361 114 71 120 64 aact:AA01181|5end_conserved_hypothetical_protein_808774..809004_POSITIVE 356 150 106 150 102 aact:AA00674|5end_nicotinate-nucleotide-dimethylbenzimidazole_phosphoribosyltransferase_461810..462040_POSITIVE 353 120 76 156 104 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 348 114 72 80 31 aact:AA02378|5end_hypothetical_protein_1627753..1627983_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 408 120 81 97 51 aact:AA00513|3end_pseudouridylate_synthase_(pseudouridine_synthase)_355686..355836_POSITIVE 405 114 72 45 7 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 399 128 81 104 45 aact:AA02020|3end_conserved_hypothetical_protein_(possible_tetrapyrrole_methylase_family_protein)_1374742..1374892_POSITIVE 394 129 83 96 49 aact:AA02940|3end_conserved_hypothetical_protein_2058652..2058802_POSITIVE 387 114 73 139 93 aact:AA01389|3end_hypothetical_protein_937158..937308_POSITIVE 377 120 73 115 63 aact:AA01185|3end_conserved_hypothetical_protein_(possible_membrane_protein)_813401..813551_POSITIVE 375 114 78 80 41 aact:AA02865|3end_iron-regulated_outer_membrane_protein_(omp64)_2012542..2012692_POSITIVE 365 110 65 151 91 aact:AA02667|3end_hypothetical_protein_1856814..1856964_POSITIVE 364 129 82 146 101 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 364 114 76 151 115 aact:AA00267|3end_hypothetical_protein_188143..188293_POSITIVE 361 114 71 67 11 aact:AA01179|3end_beta-ketoacyl-ACP_reductase;_short-chain_alcohol_dehydrogenase_808801..808951_POSITIVE 353 120 76 151 99 aact:AA00323|3end_conserved_hypothetical_protein_(possible_SAM-dependent_methyltransferase)_220222..220372_POSITIVE 351 111 72 151 116 aact:AA02143|3end_hypothetical_protein_1467641..1467791_POSITIVE 344 118 71 77 18 aact:AA01057|3end_DnaJ-like_protein_djlA_735047..735197_POSITIVE 343 119 73 85 25 aact:AA00191|3end_conserved_hypothetical_protein_(possible_acetyltransferase,_GNAT_family)_135545..135695_POSITIVE 342 75 31 70 28 aact:AA02872|3end_conserved_hypothetical_protein_2016182..2016332_POSITIVE 341 114 71 121 78 aact:AA01044|3end_A/G-specific_adenine_glycosylase_727978..728128_POSITIVE 333 119 72 112 51 aact:AA01614|3end_D-isomer_specific_2-hydroxyacid_dehydrogenase_family_protein_1090719..1090869_POSITIVE 332 118 77 93 44 aact:AA02090|3end_glucose-1-phosphate_adenylyltransferase_1424549..1424699_POSITIVE 332 125 83 80 39 aact:AA01611|3end_anthranilate_synthase_component_II_1089619..1089769_POSITIVE