Origin IGS: tagagtttcatttagtgccaaatttatggtatagttcgacgcgggtttcactgtggcttatcattatcactttacaaataattctctttattgaaatgtgaattgaaaaaccaataatccacaatatagcaaaacaagattccgactgtattactttagatttatttagcgagatccgtaaagttaagcatgcaaaaaccaatctattttaggcgggaaaaaccaattcgaagtaataaatgctctcagttaaaatgcggtaaatcctgtgggtaaaaacgtgtgaagctagagttgcacacgggtttggttcagcagttatgaggatgtgtgccaacacgaaacctaaaccgtttcgagttaatcgtaacagttttacaagatgattttagtgttaagaagttaagaaaatagggaacaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttgttccctattttcttaacttcttaacactaaaatcatcttgtaaaactgttacgattaactcgaaacggtttaggtttcgtgttggcacacatcctcataactgctgaaccaaacccgtgtgcaactctagcttcacacgtttttacccacaggatttaccgcattttaactgagagcatttattacttcgaattggtttttcccgcctaaaatagattggtttttgcatgcttaactttacggatctcgctaaataaatctaaagtaatacagtcggaatcttgttttgctatattgtggattattggtttttcaattcacatttcaataaagagaattatttgtaaagtgataatgataagccacagtgaaacccgcgtcgaactataccataaatttggcactaaatgaaactcta Mask Tandem Repeat Region ================================================ tagagtttcatttagtgccaaatttatggtatagttcgacgcgggtttcactgtggcttatcattatcactttacaaataattctctttattgaaatgtgaattgaaaaaccaataatccacaatatagcaaaacaagattccgactgtattactttagatttatttagcgagatccgtaaagttaagcatgcaaaaaccaatctattttaggcgggaaaaaccaattcgaagtaataaatgctctcagttaaaatgcggtaaatcctgtgggtaaaaacgtgtgaagctagagttgcacacgggtttggttcagcagttatgaggatgtgtgccaacacgaaacctaaaccgtttcgagttaatcgtaacagttttacaagatgattttagtgttaagaagttaagaaaatagggaacaaa Find is-nt database================================================ Query_seq: AA02514:AA02515|AA02514:AA02515:esterase/lipase:flavodoxin:->->:1748439..1748860 422 tagagtttcatttagtgccaaatttatggtatagttcgacgcgggtttcactgtggcttatcattatcactttacaaataattctctttattgaaatgtgaattgaaaaaccaataatccacaatatagcaaaacaagattccgactgtattactttagatttatttagcgagatccgtaaagttaagcatgcaaaaaccaatctattttaggcgggaaaaaccaattcgaagtaataaatgctctcagttaaaatgcggtaaatcctgtgggtaaaaacgtgtgaagctagagttgcacacgggtttggttcagcagttatgaggatgtgtgccaacacgaaacctaaaccgtttcgagttaatcgtaacagttttacaagatgattttagtgttaagaagttaagaaaatagggaacaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02514:AA02515|AA02514:AA02515:esterase/lipase:flavodoxin:->->:1748439..1748860 422 tagagtttcatttagtgccaaatttatggtatagttcgacgcgggtttcactgtggcttatcattatcactttacaaataattctctttattgaaatgtgaattgaaaaaccaataatccacaatatagcaaaacaagattccgactgtattactttagatttatttagcgagatccgtaaagttaagcatgcaaaaaccaatctattttaggcgggaaaaaccaattcgaagtaataaatgctctcagttaaaatgcggtaaatcctgtgggtaaaaacgtgtgaagctagagttgcacacgggtttggttcagcagttatgaggatgtgtgccaacacgaaacctaaaccgtttcgagttaatcgtaacagttttacaagatgattttagtgttaagaagttaagaaaatagggaacaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 126 Max: 393 Len: 268 Subject: gi|89107543|ref|AP_001323.1| LexA regulated protein [Escherichia coli W3110] HSP 1 e-value: 9.0E-6 bit: 39.3 Len: 180 Query Start:214 Query End:393 Subject Strand: null Subject Start: 30 Subject End: 89 ..................................................................................................................................................................................................................... R E K P I R S N K C S Q L K C G K S C G * K R V K L E L H T G L V Q Q L * G C V P T R N L N R F E L I V T V L Q D D F S ............................. ..................................................................................................................................................................................................................... R D E Q L R I N K R N Q L K R D K V R G L K R V E L K L N A E A V E A L N E L A E S R N M S R S E L I E E M L M Q Q L A ............................. HSP 2 e-value: 9.0E-6 bit: 26.2 Len: 78 Query Start:126 Query End:203 Subject Strand: null Subject Start: 1 Subject End: 26 ............................................................................................................................. I A K Q D S D C I T L D L F S E I R K V K H A K T N ........................................................................................................................................................................................................................... ............................................................................................................................. M A K E Q T D R T T L D L F A H E R R P G R P K T N ........................................................................................................................................................................................................................... Find nr database================================================ Query_seq: AA02514:AA02515|AA02514:AA02515:esterase/lipase:flavodoxin:->->:1748439..1748860 422 tagagtttcatttagtgccaaatttatggtatagttcgacgcgggtttcactgtggcttatcattatcactttacaaataattctctttattgaaatgtgaattgaaaaaccaataatccacaatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnctattttaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngttaagaagttaagaaaatagggaacaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagagtttcatttagtgccaaatttatggtatagttcgacgcgggtttcactgtggcttatcattatcactttacaaataattctctttattgaaatgtgaattgaaaaaccaataatccacaatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnctattttaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngttaagaagttaagaaaatagggaacaaa Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 102 End: 209 ..................................................................................................... M E K P I I H N I A K Q D S D C I T L D L F S E I R K V K H A K T N L F ..................................................................................................................................................................................................................... Protein_Len: 38 Strand: + Start: 160 End: 273 ............................................................................................................................................................... M Y L A R S V K L S M Q K P I Y F R R E K P I R S N K C S Q L K C G K S C G ..................................................................................................................................................... Protein_Len: 40 Strand: + Start: 264 End: 383 ....................................................................................................................................................................................................................................................................... M L W V K T C E A R V A H G F G S A V M R M C A N T K P K P F R V N R N S F T R ....................................... Protein_Len: 54 Strand: - Start: 212 End: 373 ................................................................................................................................................................................................................... A P F V L E F Y Y I S E T L I R Y I R H T F V H S A L T A C P N P E A T I L I H A L V F G L G N R T L R L M ................................................. Protein_Len: 60 Strand: - Start: 11 End: 202 .......... K H Y L E V R T E S H S I M I V K C I I R K I S I H I S F G I I W L I A F C S E S Q I V K S K N L S I R L T L C A F V M ............................................................................................................................................................................................................................ tagagtttcatttagtgccaaatttatggtatagttcgacgcgggtttcactgtggcttatcattatcactttacaaataattctctttattgaaatgtgaattgaaaaaccaataatccacaatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnctattttaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngttaagaagttaagaaaatagggaacaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 6 End: 35 .....TTTCATTTAGTGCCAAATTTATGGTATAGT................................................................................................................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagagtttcatttagtgccaaatttatggtatagttcgacgcgggtttcactgtggcttatcattatcactttacaaataattctctttattgaaatgtgaattgaaaaaccaataatccacaatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnctattttaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngttaagaagttaagaaaatagggaacaaa Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 71 HP_score: -5.2 Tail_Score: -4.56597 Start: 280 End: 303 Full_Region: ctgctgaaccaaacc cgtgtgcaac tcta gcttcacacg tttttacccacagga .......................................................................................................................................................................................................................................................................................cgtgtgcaactctagcttcacacg....................................................................................................................... TransTerm Strand: + Conf: 47 HP_score: -3.4 Tail_Score: -3.21595 Start: 338 End: 360 Full_Region: GAGGATGTGTGCCAA CACGAAAC CTAAACC GTTTCGAG TTAATCGTAACAGTT .................................................................................................................................................................................................................................................................................................................................................CACGAAACCTAAACCGTTTCGAG.............................................................. TransTerm Strand: - Conf: 50 HP_score: -4.6 Tail_Score: -3.02144 Start: 340 End: 358 Full_Region: ctgttacgattaact cgaaac ggtttag gtttcg tgttggcacacatcc ...................................................................................................................................................................................................................................................................................................................................................cgaaacggtttaggtttcg................................................................ TransTerm Strand: - Conf: 56 HP_score: -4.1 Tail_Score: -3.88823 Start: 351 End: 373 Full_Region: aatcatcttgtaaaa ctgttacga ttaac tcgaaacgg tttaggtttcgtgtt ..............................................................................................................................................................................................................................................................................................................................................................ctgttacgattaactcgaaacgg................................................. Find igs database================================================ Query_seq: AA02514:AA02515|AA02514:AA02515:esterase/lipase:flavodoxin:->->:1748439..1748860 422 tagagtttcatttagtgccaaatttatggtatagttcgacgcgggtttcactgtggcttatcattatcactttacaaataattctctttattgaaatgtgaattgaaaaaccaataatccacaatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnctattttaggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngttaagaagttaagaaaatagggaacaaa Intra-Species Hit: Count: 1 Min: 1 Max: 422 Len: 422 Subject: aact_AA02514_AA02515|esterase/lipase:flavodoxin|POSITIVE:POSITIVE|[ 1748439,1748860]|422 HSP 1 e-value: 1.0E-64 bit: 248.0 Len: 125 Query Start:1 Query End:125 Subject Strand: POSITIVE Subject Start: 1 Subject End: 125 tagagtttcatttagtgccaaatttatggtatagttcgacgcgggtttcactgtggcttatcattatcactttacaaataattctctttattgaaatgtgaattgaaaaaccaataatccacaat......................................................................................................................................................................................................................................................................................................... tagagtttcatttagtgccaaatttatggtatagttcgacgcgggtttcactgtggcttatcattatcactttacaaataattctctttattgaaatgtgaattgaaaaaccaataatccacaat......................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-6 bit: 56.0 Len: 28 Query Start:395 Query End:422 Subject Strand: POSITIVE Subject Start: 395 Subject End: 422 ..........................................................................................................................................................................................................................................................................................................................................................................................................ttaagaagttaagaaaatagggaacaaa ..........................................................................................................................................................................................................................................................................................................................................................................................................ttaagaagttaagaaaatagggaacaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAGUUUCAUUUAGUGCCAAAUUUAUGGUAUAGUUCGACGCGGGUUUCACUGUGGCUUAUCAUUAUCACUUUACAAAUAAUUCUCUUUAUUGAAAUGUGAAUUGAAAAACCAAUAAUCCACAAUAUAGCAAAACAAGAUUCCGACUGUAUUACUUUAGAUUUAUUUAGCGAGAUCCGUAAAGUUAAGCAUGCAAAAACCAAUCUAUUUUAGGCGGGAAAAACCAAUUCGAAGUAAUAAAUGCUCUCAGUUAAAAUGCGGUAAAUCCUGUGGGUAAAAACGUGUGAAGCUAGAGUUGCACACGGGUUUGGUUCAGCAGUUAUGAGGAUGUGUGCCAACACGAAACCUAAACCGUUUCGAGUUAAUCGUAACAGUUUUACAAGAUGAUUUUAGUGUUAAGAAGUUAAGAAAAUAGGGAACAAA ....(((((.....((((((......)))))).....(((((.((((((....((((.((((....(((((((...(((((......))))).))).))))((((...((((((..(((((((.....(.....)..((((...(((((((((((..(((((((((...((((..((.((((((.....................)))))))).((......))..))))....))))))).))...)))...)))))))).)))).))))))).....((((((.(((....))).))))))...))))))...))))......))))...))))....)))))).((((.(((.(((.....))).))).))))...............)))))..................)))))... (-87.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUGUUCCCUAUUUUCUUAACUUCUUAACACUAAAAUCAUCUUGUAAAACUGUUACGAUUAACUCGAAACGGUUUAGGUUUCGUGUUGGCACACAUCCUCAUAACUGCUGAACCAAACCCGUGUGCAACUCUAGCUUCACACGUUUUUACCCACAGGAUUUACCGCAUUUUAACUGAGAGCAUUUAUUACUUCGAAUUGGUUUUUCCCGCCUAAAAUAGAUUGGUUUUUGCAUGCUUAACUUUACGGAUCUCGCUAAAUAAAUCUAAAGUAAUACAGUCGGAAUCUUGUUUUGCUAUAUUGUGGAUUAUUGGUUUUUCAAUUCACAUUUCAAUAAAGAGAAUUAUUUGUAAAGUGAUAAUGAUAAGCCACAGUGAAACCCGCGUCGAACUAUACCAUAAAUUUGGCACUAAAUGAAACUCUA ......................................(((((((.((((((((.(((.....))).)))))))).(((((.((.(..(((..............)))..))).)))))((((((...(....)...)))))).........)))))))................(((..((((((....(((((...((((((.(....(((...)))..((((((...(((.....((((((((((((((...............(((((.((((........)))).)))))....(((((((...(((....))))))))))..........))))....))))))))))....)))..))))))..).))))))....))))).....(((......)))...))))))...))).. (-77.94) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 335 334 297 217 174 aact:AA02585|5end_hypothetical_protein_1798798..1799028_POSITIVE 332 57 15 151 101 aact:AA00395|5end_conserved_hypothetical_protein_265278..265508_POSITIVE 309 333 292 100 62 aact:AA01201|5end_phosphatase;_hydrolase_822513..822743_POSITIVE 308 368 321 198 135 aact:AA00991|5end_conserved_hypothetical_protein_693191..693421_POSITIVE 305 289 241 218 156 aact:AA00535|5end_hypothetical_protein_365695..365925_POSITIVE 304 309 266 82 43 aact:AA02168|5end_possible_protease;_heat_shock_protein_1483529..1483759_POSITIVE 304 57 15 202 163 aact:AA00549|5end_ferredoxin-type_protein_375563..375793_POSITIVE 302 317 271 51 11 aact:AA00546|5end_cytochrome_C-type_protein_(denitrification_system_component)_374427..374657_POSITIVE 301 327 281 60 6 aact:AA02597|5end_ribonuclease_D_1807124..1807354_POSITIVE 297 258 211 161 111 aact:AA02109|5end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1436563..1436793_POSITIVE 296 57 15 51 5 aact:AA00229|5end_DNA_polymerase_III,_alpha_subunit_156863..157093_POSITIVE 294 300 257 100 59 aact:AA01437|5end_phosphoglycerate_kinase_964125..964355_POSITIVE 293 327 283 212 167 aact:AA02618|5end_phosphatidylglycerophosphatase_B_1824649..1824879_POSITIVE 293 333 294 105 67 aact:AA02512|5end_O-succinylbenzoate--CoA_ligase_1745379..1745609_POSITIVE 292 259 211 82 42 aact:AA02524|5end_orotidine_5'-phosphate_decarboxylase_1753313..1753543_POSITIVE 290 326 281 190 134 aact:AA00761|5end_conserved_hypothetical_protein_(possible_peptidase)_523554..523784_POSITIVE 287 359 311 126 68 aact:AA02933|5end_conserved_hypothetical_protein_(HesA/MoeB/ThiF_family_protein)_2053467..2053697_POSITIVE 285 334 294 224 179 aact:AA02686|5end_hydrogenase_4_component_C_1871546..1871776_POSITIVE 285 310 264 222 156 aact:AA01648|5end_alpha-amylase_protein;_1,4-alpha-D-glucan_glucanohydrolase_1115170..1115400_POSITIVE 285 310 264 121 55 aact:AA01646|5end_hypothetical_protein_1115069..1115299_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 335 334 297 68 25 aact:AA02584|3end_ADP-heptose_synthase_1798729..1798879_POSITIVE 295 335 302 45 3 aact:AA02609|3end_ABC_transporter,_ATP-binding_protein/permease_1820184..1820334_POSITIVE 293 333 294 93 55 aact:AA02511|3end_transmembrane_protein_1745447..1745597_POSITIVE 292 259 211 73 33 aact:AA02523|3end_conserved_hypothetical_protein_1753384..1753534_POSITIVE 288 59 20 68 25 aact:AA01467|3end_mannose-specific_phosphotransferase_system_IID_987214..987364_POSITIVE 287 359 311 123 65 aact:AA02932|3end_lytic_murein_transglycosylase_A_2053544..2053694_POSITIVE 280 57 26 149 119 aact:AA02173|3end_anthranilate_synthase_component_I_1488365..1488515_POSITIVE 279 330 283 51 9 aact:AA00448|3end_C-terminal_region_of_competence_protein_M_313058..313208_POSITIVE 277 348 306 90 49 aact:AA01923|3end_type_III_restriction-modification_system_methylation_subunit_1299936..1300086_POSITIVE 277 342 302 64 29 aact:AA01041|3end_hypothetical_protein_725055..725205_POSITIVE 277 59 15 86 51 aact:AA00425|3end_N-terminal_region_of_competence_protein_M_288073..288223_POSITIVE 277 361 321 120 82 aact:AA00349|3end_hypothetical_protein_233527..233677_POSITIVE 277 346 302 148 101 aact:AA00218|3end_argininosuccinate_lyase_152211..152361_POSITIVE 276 60 15 147 101 aact:AA01763|3end_conserved_hypothetical_protein_(possible_transcriptional_regulatory_protein)_1190818..1190968_POSITIVE 276 75 37 41 1 aact:AA01566|3end_conserved_hypothetical_protein_1052091..1052241_POSITIVE 276 75 37 41 1 aact:AA01261|3end_conserved_hypothetical_protein_856723..856873_POSITIVE 275 268 223 143 93 aact:AA02425|3end_glutamate-1-semialdehyde_aminotransferase_1671661..1671811_POSITIVE 275 366 326 105 56 aact:AA02001|3end_conserved_hypothetical_protein_1360239..1360389_POSITIVE 274 56 17 47 9 aact:AA02757|3end_histidinol-phosphate_aminotransferase_2_1931808..1931958_POSITIVE 273 59 14 45 11 aact:AA01295|3end_3-dehydroquinate_synthase_878437..878587_POSITIVE