Origin IGS: aaaagccttcatcatttggaaaaaatatttaaaaattatgatataaacagatagtaattatatctaatgacaggataagtatagaagtgaataactgtaaagtacagtaaaacaccaaagaaagataataaatagaatggattcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagaatccattctatttattatctttctttggtgttttactgtactttacagttattcacttctatacttatcctgtcattagatataattactatctgtttatatcataatttttaaatattttttccaaatgatgaaggctttt Mask Tandem Repeat Region ================================================ aaaagccttcatcatttggaaaaaatatttaaaaattatgatataaacagatagtaattatatctaatgacaggataagtatagaagtgaataactgtaaagtacagtaaaacaccaaagaaagataataaatagaatggattcta Find is-nt database================================================ Query_seq: AA02499:AA02500|AA02499:AA02500:dolichol phosphate mannose:conserved hypothetical protein:->->:1734605..1734750 146 aaaagccttcatcatttggaaaaaatatttaaaaattatgatataaacagatagtaattatatctaatgacaggataagtatagaagtgaataactgtaaagtacagtaaaacaccaaagaaagataataaatagaatggattcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02499:AA02500|AA02499:AA02500:dolichol phosphate mannose:conserved hypothetical protein:->->:1734605..1734750 146 aaaagccttcatcatttggaaaaaatatttaaaaattatgatataaacagatagtaattatatctaatgacaggataagtatagaagtgaataactgtaaagtacagtaaaacaccaaagaaagataataaatagaatggattcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02499:AA02500|AA02499:AA02500:dolichol phosphate mannose:conserved hypothetical protein:->->:1734605..1734750 146 aaaagccttcatcatttggaaaaaatatttaaaaattatgatataaacagatagtaattatatctaatgacaggataagtatagaagtgaataactgtaaagtacagtaaaacaccaaagaaagataataaatagaatggattcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaagccttcatcatttggaaaaaatatttaaaaattatgatataaacagatagtaattatatctaatgacaggataagtatagaagtgaataactgtaaagtacagtaaaacaccaaagaaagataataaatagaatggattcta Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: - Start: 32 End: 142 ............................... F N H Y L C I T I I D L S L I L I S T F L Q L T C Y F V L S L Y Y I S H M .... aaaagccttcatcatttggaaaaaatatttaaaaattatgatataaacagatagtaattatatctaatgacaggataagtatagaagtgaataactgtaaagtacagtaaaacaccaaagaaagataataaatagaatggattcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaagccttcatcatttggaaaaaatatttaaaaattatgatataaacagatagtaattatatctaatgacaggataagtatagaagtgaataactgtaaagtacagtaaaacaccaaagaaagataataaatagaatggattcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02499:AA02500|AA02499:AA02500:dolichol phosphate mannose:conserved hypothetical protein:->->:1734605..1734750 146 aaaagccttcatcatttggaaaaaatatttaaaaattatgatataaacagatagtaattatatctaatgacaggataagtatagaagtgaataactgtaaagtacagtaaaacaccaaagaaagataataaatagaatggattcta Intra-Species Hit: Count: 1 Min: 1 Max: 146 Len: 146 Subject: aact_AA02499_AA02500|dolichol phosphate mannose:conserved hypothetical protein|POSITIVE:POSITIVE|[1734605,1734750]|146 HSP 1 e-value: 1.0E-77 bit: 289.0 Len: 146 Query Start:1 Query End:146 Subject Strand: POSITIVE Subject Start: 1 Subject End: 146 aaaagccttcatcatttggaaaaaatatttaaaaattatgatataaacagatagtaattatatctaatgacaggataagtatagaagtgaataactgtaaagtacagtaaaacaccaaagaaagataataaatagaatggattcta aaaagccttcatcatttggaaaaaatatttaaaaattatgatataaacagatagtaattatatctaatgacaggataagtatagaagtgaataactgtaaagtacagtaaaacaccaaagaaagataataaatagaatggattcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAGCCUUCAUCAUUUGGAAAAAAUAUUUAAAAAUUAUGAUAUAAACAGAUAGUAAUUAUAUCUAAUGACAGGAUAAGUAUAGAAGUGAAUAACUGUAAAGUACAGUAAAACACCAAAGAAAGAUAAUAAAUAGAAUGGAUUCUA ...........((.(((((.....(((((((...((((.(((((((((.....))..))))))))))).......)))))))...........((((((...))))))......)))))))...........(((((....))))) (-17.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAAUCCAUUCUAUUUAUUAUCUUUCUUUGGUGUUUUACUGUACUUUACAGUUAUUCACUUCUAUACUUAUCCUGUCAUUAGAUAUAAUUACUAUCUGUUUAUAUCAUAAUUUUUAAAUAUUUUUUCCAAAUGAUGAAGGCUUUU (((((....)))))........(((((...((((....((((((...))))))....))))..............((((((.(((((((..((.....)))))))))...(((....)))..........)))))))))))..... (-18.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 230 121 87 139 108 aact:AA00660|5end_hypothetical_protein_453031..453261_POSITIVE 208 109 61 52 6 aact:AA00998|5end_small_protein_A_homolog_698525..698755_POSITIVE 204 127 101 109 85 aact:AA01024|5end_thiamine_biosynthesis_lipoprotein_precursor_716437..716667_POSITIVE 189 115 71 222 180 aact:AA01319|5end_transcriptional_regulatory_protein_892452..892682_POSITIVE 185 130 84 162 108 aact:AA01092|5end_hypothetical_protein_756114..756344_POSITIVE 184 120 72 106 53 aact:AA00515|5end_hypothetical_protein_355730..355960_POSITIVE 182 74 38 102 73 aact:AA02826|5end_conserved_hypothetical_protein_1986336..1986566_POSITIVE 182 106 65 119 78 aact:AA01500|5end_hypothetical_protein_1009716..1009946_POSITIVE 180 122 84 67 26 aact:AA00692|5end_conserved_hypothetical_protein_473236..473466_POSITIVE 172 107 75 162 131 aact:AA02718|5end_spermidine/putrescine_ABC_transporter,_ATP-binding_protein_1901294..1901524_POSITIVE 170 110 68 204 175 aact:AA01662|5end_molybdopterin_biosynthesis_protein_1126131..1126361_POSITIVE 170 140 95 61 13 aact:AA01124.1|5end_biopolymer_transport_protein_D_772229..772459_POSITIVE 169 110 71 162 126 aact:AA02604|5end_thioredoxin_reductase_1815552..1815782_POSITIVE 165 89 49 123 82 aact:AA02412|5end_cysteine_synthetase_1657528..1657758_POSITIVE 165 117 71 67 23 aact:AA00046|5end_formate_dependent_nitrite_reductase,_cytochrome-c_type_protein_36014..36244_POSITIVE 164 109 68 93 57 aact:AA00422|5end_LapB_membrane_protein_285528..285758_POSITIVE 163 107 66 148 109 aact:AA01507|5end_conserved_hypothetical_protein_1012433..1012663_POSITIVE 162 117 71 169 118 aact:AA01432|5end_lysophospholipase_L2_960715..960945_POSITIVE 160 111 71 181 140 aact:AA02450|5end_conserved_hypothetical_protein_1687701..1687931_POSITIVE 160 111 71 55 14 aact:AA02449|5end_hypothetical_protein_1687575..1687805_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 230 121 87 55 24 aact:AA00659|3end_heat_shock_protein_453027..453177_POSITIVE 204 127 101 27 3 aact:AA01023|3end_conserved_hypothetical_protein_716435..716585_POSITIVE 189 115 71 103 61 aact:AA01318|3end_hypothetical_protein_892413..892563_POSITIVE 186 90 43 94 44 aact:AA00414|3end_possible_histidinol-phosphatase_281533..281683_POSITIVE 184 120 72 134 81 aact:AA00514|3end_hypothetical_protein_355838..355988_POSITIVE 182 74 38 85 56 aact:AA02824|3end_conserved_hypothetical_protein_1986399..1986549_POSITIVE 172 107 75 100 69 aact:AA02717|3end_hypothetical_protein_1901312..1901462_POSITIVE 171 108 73 31 2 aact:AA02354|3end_conserved_hypothetical_protein_1613008..1613158_POSITIVE 170 140 95 54 6 aact:AA01124|3end_biopolymer_transport_protein_772302..772452_POSITIVE 170 106 61 140 101 aact:AA00616|3end_phosphatidate_cytidylyltransferase_422971..423121_POSITIVE 169 110 71 99 63 aact:AA02603|3end_possible_thioredoxin_trxA_homolog_1815569..1815719_POSITIVE 166 117 86 40 14 aact:AA01904|3end_hypothetical_protein_1285049..1285199_POSITIVE 166 24 1 141 114 aact:AA01169|3end_hypothetical_protein_802229..802379_POSITIVE 165 117 71 67 23 aact:AA00044|3end_formate_dependent_nitrite_reductase,_Fe-S_protein/_anaerobic_DMSO_reductase_B_36094..36244_POSITIVE 163 107 66 129 90 aact:AA01506|3end_protein-export_protein_1012494..1012644_POSITIVE 162 117 71 125 74 aact:AA01431|3end_conserved_hypothetical_protein_(possible_hydrolase)_960751..960901_POSITIVE 161 102 63 124 86 aact:AA02713|3end_possible_prop_effector_homolog_1895669..1895819_POSITIVE 159 89 47 67 28 aact:AA01778|3end_aminopeptidase_A/I;_cytosol_aminopeptidase;_leucyl_aminopeptidase_1199965..1200115_POSITIVE 158 88 45 122 73 aact:AA01738|3end_enoyl-(acyl-carrier-protein)_reductase_1175564..1175714_POSITIVE 157 101 67 54 22 aact:AA02301|3end_chorismate_lyase_1572795..1572945_POSITIVE