Origin IGS: aattgagactagttatcagttgaaaagtttaagaattttagaggtttttctggaagattgatataatccttacgaattattttcattacggagaagaaatc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gatttcttctccgtaatgaaaataattcgtaaggattatatcaatcttccagaaaaacctctaaaattcttaaacttttcaactgataactagtctcaatt Mask Tandem Repeat Region ================================================ aattgagactagttatcagttgaaaagtttaagaattttagaggtttttctggaagattgatataatccttacgaattattttcattacggagaagaaatc Find is-nt database================================================ Query_seq: AA02494:AA02495|AA02494:AA02495:conserved hypothetical protein:conserved hypothetical protein:->->:1731601..1731701 101 aattgagactagttatcagttgaaaagtttaagaattttagaggtttttctggaagattgatataatccttacgaattattttcattacggagaagaaatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02494:AA02495|AA02494:AA02495:conserved hypothetical protein:conserved hypothetical protein:->->:1731601..1731701 101 aattgagactagttatcagttgaaaagtttaagaattttagaggtttttctggaagattgatataatccttacgaattattttcattacggagaagaaatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02494:AA02495|AA02494:AA02495:conserved hypothetical protein:conserved hypothetical protein:->->:1731601..1731701 101 aattgagactagttatcagttgaaaagtttaagaattttagaggtttttctggaagattgatataatccttacgaattattttcattacggagaagaaatc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aattgagactagttatcagttgaaaagtttaagaattttagaggtttttctggaagattgatataatccttacgaattattttcattacggagaagaaatc Predict ORF larger than 30AA ================================================ aattgagactagttatcagttgaaaagtttaagaattttagaggtttttctggaagattgatataatccttacgaattattttcattacggagaagaaatc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aattgagactagttatcagttgaaaagtttaagaattttagaggtttttctggaagattgatataatccttacgaattattttcattacggagaagaaatc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02494:AA02495|AA02494:AA02495:conserved hypothetical protein:conserved hypothetical protein:->->:1731601..1731701 101 aattgagactagttatcagttgaaaagtttaagaattttagaggtttttctggaagattgatataatccttacgaattattttcattacggagaagaaatc Intra-Species Hit: Count: 1 Min: 1 Max: 101 Len: 101 Subject: aact_AA02494_AA02495|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1731601,1731701]|101 HSP 1 e-value: 6.0E-51 bit: 200.0 Len: 101 Query Start:1 Query End:101 Subject Strand: POSITIVE Subject Start: 1 Subject End: 101 aattgagactagttatcagttgaaaagtttaagaattttagaggtttttctggaagattgatataatccttacgaattattttcattacggagaagaaatc aattgagactagttatcagttgaaaagtttaagaattttagaggtttttctggaagattgatataatccttacgaattattttcattacggagaagaaatc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUGAGACUAGUUAUCAGUUGAAAAGUUUAAGAAUUUUAGAGGUUUUUCUGGAAGAUUGAUAUAAUCCUUACGAAUUAUUUUCAUUACGGAGAAGAAAUC .....(((((..(((.....)))..)))))..........((..((((((((...(((((...))))).....(((.....)))....))))))))...)) (-11.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUUUCUUCUCCGUAAUGAAAAUAAUUCGUAAGGAUUAUAUCAAUCUUCCAGAAAAACCUCUAAAAUUCUUAAACUUUUCAACUGAUAACUAGUCUCAAUU ((((..((.((.((..((((((((((((....)))))))...........(((......)))..............))))))).)).))..))))...... (-11.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 232 50 1 87 37 aact:AA01135|5end_possible_cell_filamentation_protein_777381..777611_POSITIVE 228 78 37 42 2 aact:AA01705|5end_beta-galactosidase_1157643..1157873_POSITIVE 210 60 17 86 43 aact:AA02215|5end_conserved_hypothetical_protein_1508800..1509030_POSITIVE 205 56 18 126 91 aact:AA00845|5end_hypothetical_protein_584531..584761_POSITIVE 203 69 26 141 89 aact:AA02289|5end_cell_division_protein_H,_a_metalloprotease_1559223..1559453_POSITIVE 193 72 34 126 93 aact:AA00706|5end_tRNA/rRNA_methyltransferase_484712..484942_POSITIVE 190 60 21 185 139 aact:AA02796|5end_possible_transcriptional_regulator,_RpiR_family_1963001..1963231_POSITIVE 190 84 41 52 13 aact:AA01895|5end_hypothetical_protein_1279574..1279804_POSITIVE 190 74 31 108 51 aact:AA00002|5end_DNA_polymerase_III,_beta_subunit_1233..1463_POSITIVE 188 75 32 180 133 aact:AA02002|5end_hypothetical_protein_1360196..1360426_POSITIVE 187 76 33 229 187 aact:AA00107|5end_histidyl-tRNA_synthetase_76699..76929_POSITIVE 184 50 12 124 83 aact:AA01737|5end_exoribonuclease_II_1172647..1172877_POSITIVE 182 100 58 135 92 aact:AA00583|5end_GlpG_protein_398258..398488_POSITIVE 178 74 38 229 194 aact:AA02163|5end_integration_host_factor_alpha-subunit_1479490..1479720_POSITIVE 177 100 55 124 71 aact:AA00825|5end_P-protein_(chorismate_mutase/prephenate_dehydratase)_567020..567250_POSITIVE 177 99 51 88 30 aact:AA00044|5end_formate_dependent_nitrite_reductase,_Fe-S_protein/_anaerobic_DMSO_reductase_B_35340..35570_POSITIVE 176 97 52 180 135 aact:AA01476|5end_hypothetical_protein_991402..991632_POSITIVE 175 59 11 77 31 aact:AA02629|5end_hypothetical_protein_1830072..1830302_POSITIVE 174 76 42 144 102 aact:AA00519|5end_O-acetyltransferase_357139..357369_POSITIVE 174 87 41 229 188 aact:AA00429|5end_conserved_hypothetical_protein_290567..290797_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 205 56 18 58 23 aact:AA00844|3end_cell_division_protein_I;_penicillin_binding_protein_2X_584543..584693_POSITIVE 193 72 34 38 5 aact:AA00705|3end_Inositol-1-monophosphatase_(IMPase);_extragenic_suppressor_484704..484854_POSITIVE 190 60 21 53 7 aact:AA02794|3end_myo-inositol_catabolism_protein_1962949..1963099_POSITIVE 190 74 31 94 37 aact:AA00001|3end_chromosomal_replication_initiator_protein_1299..1449_POSITIVE 188 75 32 143 96 aact:AA02001|3end_conserved_hypothetical_protein_1360239..1360389_POSITIVE 184 50 12 111 70 aact:AA01735|3end_hypothetical_protein_1172714..1172864_POSITIVE 182 100 58 92 49 aact:AA00582|3end_conserved_hypothetical_protein_398295..398445_POSITIVE 177 100 55 149 96 aact:AA00824|3end_hypothetical_protein_567125..567275_POSITIVE 177 99 51 88 30 aact:AA00043|3end_formate_dependent_nitrite_reductase,_transmembrane_protein_35420..35570_POSITIVE 176 97 52 128 83 aact:AA01475|3end_conserved_hypothetical_protein_(possible_cytoplasmic_protein;_DNA_binding_protein)_991430..991580_POSITIVE 174 76 42 138 96 aact:AA00518|3end_conserved_hypothetical_protein_357213..357363_POSITIVE 172 63 49 17 3 aact:AA01477|3end_type_I_restriction_enzyme_R_protein_994479..994629_POSITIVE 170 75 36 131 86 aact:AA02897|3end_oligopeptide_ABC_transporter;_permease_protein_2029150..2029300_POSITIVE 170 78 31 69 15 aact:AA02429|3end_methionine_aminopeptidase_1675197..1675347_POSITIVE 169 97 55 50 1 aact:AA00066|3end_NADP-dependent_malic_enzyme_(NADP-ME)_51343..51493_POSITIVE 168 60 15 134 98 aact:AA00513|3end_pseudouridylate_synthase_(pseudouridine_synthase)_355686..355836_POSITIVE 166 57 19 44 13 aact:AA01978|3end_hypothetical_protein_1337257..1337407_POSITIVE 165 70 28 97 54 aact:AA02834|3end_colicin_transport_protein_1991768..1991918_POSITIVE 165 74 34 58 5 aact:AA01711|3end_conserved_hypothetical_protein_1160502..1160652_POSITIVE 164 96 68 69 41 aact:AA01673|3end_riboflavin_synthase,_alpha_chain_1132093..1132243_POSITIVE