Origin IGS: tgaaaatcttaaattgcacttaaaggctgctattcgcattctttcatgaggaactgcggttatttttaccggtgtttttttctcttacaataaacaagatagaaagaattttattcactactgagtgtaactaagccggcacgttgtatatatccataaatggaaatatcaatagattcaatcaggtgggcatattacacaaatctgctacaatgtccctgatggttatttataccatcattaagcaggcaaaaattgtaattgtcattttattcaaggagttaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 attaactccttgaataaaatgacaattacaatttttgcctgcttaatgatggtataaataaccatcagggacattgtagcagatttgtgtaatatgcccacctgattgaatctattgatatttccatttatggatatatacaacgtgccggcttagttacactcagtagtgaataaaattctttctatcttgtttattgtaagagaaaaaaacaccggtaaaaataaccgcagttcctcatgaaagaatgcgaatagcagcctttaagtgcaatttaagattttca Mask Tandem Repeat Region ================================================ tgaaaatcttaaattgcacttaaaggctgctattcgcattctttcatgaggaactgcggttatttttaccggtgtttttttctcttacaataaacaagatagaaagaattttattcactactgagtgtaactaagccggcacgttgtatatatccataaatggaaatatcaatagattcaatcaggtgggcatattacacaaatctgctacaatgtccctgatggttatttataccatcattaagcaggcaaaaattgtaattgtcattttattcaaggagttaat Find is-nt database================================================ Query_seq: AA02491:AA02492|AA02491:AA02492:topoisomerase IV, subunit A:glutathione S-transferase:->->:1729220..1729505 286 tgaaaatcttaaattgcacttaaaggctgctattcgcattctttcatgaggaactgcggttatttttaccggtgtttttttctcttacaataaacaagatagaaagaattttattcactactgagtgtaactaagccggcacgttgtatatatccataaatggaaatatcaatagattcaatcaggtgggcatattacacaaatctgctacaatgtccctgatggttatttataccatcattaagcaggcaaaaattgtaattgtcattttattcaaggagttaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02491:AA02492|AA02491:AA02492:topoisomerase IV, subunit A:glutathione S-transferase:->->:1729220..1729505 286 tgaaaatcttaaattgcacttaaaggctgctattcgcattctttcatgaggaactgcggttatttttaccggtgtttttttctcttacaataaacaagatagaaagaattttattcactactgagtgtaactaagccggcacgttgtatatatccataaatggaaatatcaatagattcaatcaggtgggcatattacacaaatctgctacaatgtccctgatggttatttataccatcattaagcaggcaaaaattgtaattgtcattttattcaaggagttaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02491:AA02492|AA02491:AA02492:topoisomerase IV, subunit A:glutathione S-transferase:->->:1729220..1729505 286 tgaaaatcttaaattgcacttaaaggctgctattcgcattctttcatgaggaactgcggttatttttaccggtgtttttttctcttacaataaacaagatagaaagaattttattcactactgagtgtaactaagccggcacgttgtatatatccataaatggaaatatcaatagattcaatcaggtgggcatattacacaaatctgctacaatgtccctgatggttatttataccatcattaagcaggcaaaaattgtaattgtcattttattcaaggagttaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgaaaatcttaaattgcacttaaaggctgctattcgcattctttcatgaggaactgcggttatttttaccggtgtttttttctcttacaataaacaagatagaaagaattttattcactactgagtgtaactaagccggcacgttgtatatatccataaatggaaatatcaatagattcaatcaggtgggcatattacacaaatctgctacaatgtccctgatggttatttataccatcattaagcaggcaaaaattgtaattgtcattttattcaaggagttaat Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 27 End: 131 .......................... M L F A F F H E E L R L F L P V F F S L T I N K I E R I L F T T E C N ........................................................................................................................................................... Protein_Len: 47 Strand: + Start: 144 End: 284 ............................................................................................................................................... M Y I S I N G N I N R F N Q V G I L H K S A T M S L M V I Y T I I K Q A K I V I V I L F K E L .. tgaaaatcttaaattgcacttaaaggctgctattcgcattctttcatgaggaactgcggttatttttaccggtgtttttttctcttacaataaacaagatagaaagaattttattcactactgagtgtaactaagccggcacgttgtatatatccataaatggaaatatcaatagattcaatcaggtgggcatattacacaaatctgctacaatgtccctgatggttatttataccatcattaagcaggcaaaaattgtaattgtcattttattcaaggagttaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgaaaatcttaaattgcacttaaaggctgctattcgcattctttcatgaggaactgcggttatttttaccggtgtttttttctcttacaataaacaagatagaaagaattttattcactactgagtgtaactaagccggcacgttgtatatatccataaatggaaatatcaatagattcaatcaggtgggcatattacacaaatctgctacaatgtccctgatggttatttataccatcattaagcaggcaaaaattgtaattgtcattttattcaaggagttaat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02491:AA02492|AA02491:AA02492:topoisomerase IV, subunit A:glutathione S-transferase:->->:1729220..1729505 286 tgaaaatcttaaattgcacttaaaggctgctattcgcattctttcatgaggaactgcggttatttttaccggtgtttttttctcttacaataaacaagatagaaagaattttattcactactgagtgtaactaagccggcacgttgtatatatccataaatggaaatatcaatagattcaatcaggtgggcatattacacaaatctgctacaatgtccctgatggttatttataccatcattaagcaggcaaaaattgtaattgtcattttattcaaggagttaat Intra-Species Hit: Count: 1 Min: 1 Max: 286 Len: 286 Subject: aact_AA02491_AA02492|topoisomerase IV, subunit A:glutathione S-transferase|POSITIVE:POSITIVE|[1729220,1729505]|286 HSP 1 e-value: 1.0E-148 bit: 525.0 Len: 286 Query Start:1 Query End:286 Subject Strand: POSITIVE Subject Start: 1 Subject End: 286 tgaaaatcttaaattgcacttaaaggctgctattcgcattctttcatgaggaactgcggttatttttaccggtgnnnnnnnctcttacaataaacaagatagaaagaattttattcactactgagtgtaactaagccggcacgttgtatatatccataaatggaaatatcaatagattcaatcaggtgggcatattacacaaatctgctacaatgtccctgatggttatttataccatcattaagcaggcaaaaattgtaattgtcattttattcaaggagttaat tgaaaatcttaaattgcacttaaaggctgctattcgcattctttcatgaggaactgcggttatttttaccggtgtttttttctcttacaataaacaagatagaaagaattttattcactactgagtgtaactaagccggcacgttgtatatatccataaatggaaatatcaatagattcaatcaggtgggcatattacacaaatctgctacaatgtccctgatggttatttataccatcattaagcaggcaaaaattgtaattgtcattttattcaaggagttaat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAAAUCUUAAAUUGCACUUAAAGGCUGCUAUUCGCAUUCUUUCAUGAGGAACUGCGGUUAUUUUUACCGGUGUUUUUUUCUCUUACAAUAAACAAGAUAGAAAGAAUUUUAUUCACUACUGAGUGUAACUAAGCCGGCACGUUGUAUAUAUCCAUAAAUGGAAAUAUCAAUAGAUUCAAUCAGGUGGGCAUAUUACACAAAUCUGCUACAAUGUCCCUGAUGGUUAUUUAUACCAUCAUUAAGCAGGCAAAAAUUGUAAUUGUCAUUUUAUUCAAGGAGUUAAU ..(((((....(((((((......(.(((((....((((((((((..(((((((..((((.......))))..)))))))..((((.........))))..)))))))................((((((.((.(((.((..((((.((((.((((....)))).)))))))).(((...)))..)).))).)))))))).....)))...........(((((((.......)))))))...))))).)......)))))))...)))))............... (-61.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUAACUCCUUGAAUAAAAUGACAAUUACAAUUUUUGCCUGCUUAAUGAUGGUAUAAAUAACCAUCAGGGACAUUGUAGCAGAUUUGUGUAAUAUGCCCACCUGAUUGAAUCUAUUGAUAUUUCCAUUUAUGGAUAUAUACAACGUGCCGGCUUAGUUACACUCAGUAGUGAAUAAAAUUCUUUCUAUCUUGUUUAUUGUAAGAGAAAAAAACACCGGUAAAAAUAACCGCAGUUCCUCAUGAAAGAAUGCGAAUAGCAGCCUUUAAGUGCAAUUUAAGAUUUUCA ...........((((.........(((((....(((((.(((((..(((((((.......)))))))..))....))))))))...(((((((..((((((..(((...)))..(((((((.((((....)))).)))).))).)))..)))...)))))))...))))).......)))).....((((((...((((((.(((.....(((..((((.......))))..))).)))...((((..(((.....)))..))))...)))))).))))))..... (-55.89) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 379 79 31 223 167 aact:AA02288|5end_conserved_hypothetical_protein_1558590..1558820_POSITIVE 356 80 32 170 128 aact:AA02899|5end_oligopeptide_ABC_transporter,_ATP-binding_protein_2030063..2030293_POSITIVE 343 59 13 202 145 aact:AA02409|5end_DNA_ligase_1653315..1653545_POSITIVE 340 60 14 162 122 aact:AA01025|5end_hypothetical_protein_717522..717752_POSITIVE 328 89 45 143 104 aact:AA02215|5end_conserved_hypothetical_protein_1508800..1509030_POSITIVE 323 78 34 63 22 aact:AA00582|5end_conserved_hypothetical_protein_397904..398134_POSITIVE 320 80 31 162 110 aact:AA02536|5end_conserved_hypothetical_protein_1764854..1765084_POSITIVE 318 79 36 127 84 aact:AA01059|5end_orotate_phosphoribosyltransferase_735052..735282_POSITIVE 314 80 55 150 125 aact:AA02885|5end_conserved_hypothetical_protein_2022428..2022658_POSITIVE 308 92 55 163 126 aact:AA01075|5end_hypothetical_protein_745605..745835_POSITIVE 305 79 36 122 79 aact:AA00198|5end_conserved_hypothetical_protein_(Cytosine_deaminase-related_enzyme)_138039..138269_POSITIVE 304 225 184 151 93 aact:AA02132|5end_dihydroorotate_dehydrogenase_1455089..1455319_POSITIVE 304 80 32 165 113 aact:AA01603|5end_oligopeptidase_A_1082898..1083128_POSITIVE 300 87 42 195 152 aact:AA01991|5end_conserved_hypothetical_protein_(possible_nitroreductase)_1352372..1352602_POSITIVE 299 58 12 177 125 aact:AA01451|5end_D-xylulokinase_971935..972165_POSITIVE 299 58 12 102 50 aact:AA01450|5end_hypothetical_protein_971860..972090_POSITIVE 298 148 111 47 18 aact:AA00223|5end_catalase_(hydroperoxidase;_iron_heme_axial_ligand)_154277..154507_POSITIVE 297 74 35 215 179 aact:AA01575|5end_conserved_hypothetical_protein_1061664..1061894_POSITIVE 296 88 55 192 164 aact:AA02162|5end_lipoprotein_1478950..1479180_POSITIVE 293 78 36 126 85 aact:AA00467|5end_hypothetical_protein_324565..324795_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 379 79 31 89 33 aact:AA02286|3end_sugar_ABC_transporter,_ATP-binding_protein_1558536..1558686_POSITIVE 366 79 31 56 9 aact:AA01107|3end_adenylosuccinate_lyase_763754..763904_POSITIVE 349 80 32 89 42 aact:AA00168|3end_possible_sigma_54_modulation_protein_120240..120390_POSITIVE 323 78 34 45 4 aact:AA00581|3end_thiosulfate_sulfurtransferase_(rhodanese)_397966..398116_POSITIVE 323 80 34 79 29 aact:AA00221|3end_NADP-specific_glutamate_dehydrogenase_153865..154015_POSITIVE 320 80 31 89 37 aact:AA02534|3end_zinc_protease_1764861..1765011_POSITIVE 314 80 55 69 44 aact:AA02883|3end_conserved_hypothetical_protein_2022427..2022577_POSITIVE 308 92 55 101 64 aact:AA01074|3end_diacylglycerol_kinase_745623..745773_POSITIVE 306 60 13 122 64 aact:AA01880|3end_N-acetylglucosamine_repressor_1268383..1268533_POSITIVE 304 225 184 134 76 aact:AA02131|3end_conserved_hypothetical_protein_(possible_TrpH_protein)_1455152..1455302_POSITIVE 296 88 55 91 63 aact:AA02160|3end_conserved_hypothetical_protein_1478929..1479079_POSITIVE 296 79 45 42 10 aact:AA01057|3end_DnaJ-like_protein_djlA_735047..735197_POSITIVE 296 67 22 64 18 aact:AA01009|3end_universal_stress_protein_A_704945..705095_POSITIVE 295 78 34 117 79 aact:AA02135|3end_hypothetical_protein_1459067..1459217_POSITIVE 295 78 34 133 95 aact:AA02135.1|3end_hypothetical_protein_1459083..1459233_POSITIVE 293 78 36 81 40 aact:AA00465|3end_hypothetical_protein_324600..324750_POSITIVE 285 79 33 79 32 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 281 78 35 115 68 aact:AA00555|3end_hypothetical_protein_380442..380592_POSITIVE 276 64 25 131 77 aact:AA02139|3end_possible_membrane_protein_1462218..1462368_POSITIVE 273 74 32 74 33 aact:AA00453|3end_phosphoglyceromutase_318009..318159_POSITIVE