Origin IGS: taattgttaatcgcgaaacacaaaaaaacccgctttcattgaaaacgggttttgttttattgatgcagccaaaaacacctaaaattctgaccgcactttaagtgaatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taattcacttaaagtgcggtcagaattttaggtgtttttggctgcatcaataaaacaaaacccgttttcaatgaaagcgggtttttttgtgtttcgcgattaacaatta Mask Tandem Repeat Region ================================================ taattgttaatcgcgaaacacaaaaaaacccgctttcattgaaaacgggttttgttttattgatgcagccaaaaacacctaaaattctgaccgcactttaagtgaatta Find is-nt database================================================ Query_seq: AA02484:AA02485|AA02484:AA02485:ABC transporter, ATP-binding protein:outer membrane protein 100:->->:1722981..1723089 109 taattgttaatcgcgaaacacaaaaaaacccgctttcattgaaaacgggttttgttttattgatgcagccaaaaacacctaaaattctgaccgcactttaagtgaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02484:AA02485|AA02484:AA02485:ABC transporter, ATP-binding protein:outer membrane protein 100:->->:1722981..1723089 109 taattgttaatcgcgaaacacaaaaaaacccgctttcattgaaaacgggttttgttttattgatgcagccaaaaacacctaaaattctgaccgcactttaagtgaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02484:AA02485|AA02484:AA02485:ABC transporter, ATP-binding protein:outer membrane protein 100:->->:1722981..1723089 109 taattgttaatcgcgaaacacaaaaaaacccgctttcattgaaaacgggttttgttttattgatgcagccaaaaacacctaaaattctgaccgcactttaagtgaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattgttaatcgcgaaacacaaaaaaacccgctttcattgaaaacgggttttgttttattgatgcagccaaaaacacctaaaattctgaccgcactttaagtgaatta Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 3 End: 98 .. M V N R E T Q K N P L S L K T G F V L L M Q P K T P K I L T A L ........... taattgttaatcgcgaaacacaaaaaaacccgctttcattgaaaacgggttttgttttattgatgcagccaaaaacacctaaaattctgaccgcactttaagtgaatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattgttaatcgcgaaacacaaaaaaacccgctttcattgaaaacgggttttgttttattgatgcagccaaaaacacctaaaattctgaccgcactttaagtgaatta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -11.9 Tail_Score: -5.88197 Start: 29 End: 49 Full_Region: atcaataaaacaaaa cccgttttc aat gaaagcggg tttttttgtgtttcg ............................cccgttttcaatgaaagcggg............................................................ Find igs database================================================ Query_seq: AA02484:AA02485|AA02484:AA02485:ABC transporter, ATP-binding protein:outer membrane protein 100:->->:1722981..1723089 109 taattgttaatcgcgaaacacaaaaaaacccgctttcattgaaaacgggttttgttttattgatgcagccaaaaacacctaaaattctgaccgcactttaagtgaatta Intra-Species Hit: Count: 1 Min: 1 Max: 109 Len: 109 Subject: aact_AA02484_AA02485|ABC transporter, ATP-binding protein:outer membrane protein 100|POSITIVE:POSITIVE|[1722981,1723089]|109 HSP 1 e-value: 4.0E-43 bit: 174.0 Len: 109 Query Start:1 Query End:109 Subject Strand: POSITIVE Subject Start: 1 Subject End: 109 taattgttaatcgcgaaacacnnnnnnncccgctttcattgaaaacgggttttgttttattgatgcagccaaaaacacctaaaattctgaccgcactttaagtgaatta taattgttaatcgcgaaacacaaaaaaacccgctttcattgaaaacgggttttgttttattgatgcagccaaaaacacctaaaattctgaccgcactttaagtgaatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUGUUAAUCGCGAAACACAAAAAAACCCGCUUUCAUUGAAAACGGGUUUUGUUUUAUUGAUGCAGCCAAAAACACCUAAAAUUCUGACCGCACUUUAAGUGAAUUA .....(((.((((.((((((.....(((((((.((((...)))).)))))))))))))..))))..)))........................(((.....)))..... (-17.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUCACUUAAAGUGCGGUCAGAAUUUUAGGUGUUUUUGGCUGCAUCAAUAAAACAAAACCCGUUUUCAAUGAAAGCGGGUUUUUUUGUGUUUCGCGAUUAACAAUUA .....(((.....((((((((((((..........)))))))))))).........(((((((((((((...)))))))))))))...))).................. (-30.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 376 70 22 185 127 aact:AA02357|5end_bifunctional_protein:_aspartokinase_I;_homoserine_dehydrogenaseI_1614668..1614898_POSITIVE 333 99 61 220 180 aact:AA02733|5end_cell_division_protein_K;_sporulation_protein_spoIIIE_1912208..1912438_POSITIVE 330 99 64 136 100 aact:AA01027|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_F_717676..717906_POSITIVE 326 99 61 109 69 aact:AA01008|5end_mazG_beta-lactamase_regulatory_protein_homolog_703470..703700_POSITIVE 326 100 65 133 94 aact:AA00075|5end_conserved_hypothetical_protein_54963..55193_POSITIVE 320 76 37 52 4 aact:AA00750|5end_periplasmic_D-galactose-binding_ABC_transport_protein_514904..515134_POSITIVE 319 100 60 45 6 aact:AA01770|5end_hypothetical_protein_1193111..1193341_POSITIVE 318 99 61 71 31 aact:AA02589|5end_NAD(P)H_oxidoreductase_1800493..1800723_POSITIVE 315 99 54 129 81 aact:AA00254|5end_penicillin-binding_protein_5_177579..177809_POSITIVE 312 99 52 156 103 aact:AA02097|5end_DedA-family_integral_membrane_protein_1428823..1429053_POSITIVE 312 99 52 87 34 aact:AA02096|5end_hypothetical_protein_1428754..1428984_POSITIVE 312 99 52 226 184 aact:AA01810|5end_electron_transport_complex_protein_1219250..1219480_POSITIVE 308 100 68 216 181 aact:AA02479|5end_outer_membrane_protein_34_1715134..1715364_POSITIVE 305 99 69 133 103 aact:AA02047|5end_galactitol-1-phosphate_5-dehydrogenase_1393633..1393863_POSITIVE 295 98 65 76 44 aact:AA00952|5end_hemY_protein_homolog_657541..657771_POSITIVE 290 99 64 112 73 aact:AA00160|5end_pilA-like_biogenesis_protein_/_prepilin_peptidase_dependent_proteinD_112836..113066_POSITIVE 289 99 65 184 150 aact:AA00679|5end_cytoplasmic_axial_filament_(Ribonuclease_G)_463288..463518_POSITIVE 282 99 61 193 144 aact:AA00268|5end_acyl_carrier_protein_(ACP)_188104..188334_POSITIVE 281 99 57 168 118 aact:AA01458|5end_aminotransferase_978820..979050_POSITIVE 280 100 53 121 77 aact:AA02106|5end_hypothetical_protein_1433241..1433471_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 376 70 22 93 35 aact:AA02356|3end_hypothetical_protein_1614656..1614806_POSITIVE 333 99 61 124 84 aact:AA02731|3end_outer_membrane_lipoprotein_carrier_precursor_1912192..1912342_POSITIVE 330 99 64 149 113 aact:AA01025|3end_hypothetical_protein_717769..717919_POSITIVE 318 99 61 68 28 aact:AA02587|3end_conserved_hypothetical_protein_1800570..1800720_POSITIVE 315 99 54 106 58 aact:AA00253|3end_hypothetical_protein_177636..177786_POSITIVE 312 99 60 89 53 aact:AA02564|3end_transketolase_N-terminal_section_1786783..1786933_POSITIVE 305 99 69 107 77 aact:AA02046|3end_PTS_system_galactitol-specific_enzyme_IIC_1393687..1393837_POSITIVE 290 99 64 131 92 aact:AA00158|3end_ampD_signalling_protein_(N-acetylmuramyl-L-alanine_amidase)_112935..113085_POSITIVE 289 99 65 64 30 aact:AA00675|3end_glutaredoxin_2_463248..463398_POSITIVE 281 99 57 76 26 aact:AA01457|3end_D-xylose_ABC_transporter,_membrane_spanning_protein_978808..978958_POSITIVE 280 100 53 90 46 aact:AA02105|3end_killing_protein_1433290..1433440_POSITIVE 276 100 55 62 24 aact:AA01945|3end_selenophosphate_synthase_1316022..1316172_POSITIVE 276 99 66 136 100 aact:AA00528|3end_conserved_hypothetical_protein_361703..361853_POSITIVE 273 98 61 151 103 aact:AA00267|3end_hypothetical_protein_188143..188293_POSITIVE 271 99 64 38 1 aact:AA02711|3end_conserved_hypothetical_protein_1892852..1893002_POSITIVE 271 100 69 37 1 aact:AA00072|3end_conserved_hypothetical_protein_54947..55097_POSITIVE 267 99 71 72 44 aact:AA02446|3end_conserved_hypothetical_protein_1687583..1687733_POSITIVE 264 99 61 81 42 aact:AA02865|3end_iron-regulated_outer_membrane_protein_(omp64)_2012542..2012692_POSITIVE 264 99 59 132 87 aact:AA01335|3end_primosomal_protein_N'_904109..904259_POSITIVE 263 58 11 71 9 aact:AA01852|3end_peptide_chain_release_factor_3_1246551..1246701_POSITIVE