Origin IGS: agtgaaattcctttattctatcgccgtgggtaataaattacccacgattatgtataattcagccccttttgggctgtataggttccccaaaggggaacaactatgcctaacctagggtaacttgttaccctaggtcattgcagtttccattaaatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatttaatggaaactgcaatgacctagggtaacaagttaccctaggttaggcatagttgttcccctttggggaacctatacagcccaaaaggggctgaattatacataatcgtgggtaatttattacccacggcgatagaataaaggaatttcact Mask Tandem Repeat Region ================================================ agtgaaattcctttattctatcgccgtgggtaataaattacccacgattatgtataattcagccccttttgggctgtataggttccccaaaggggaacaactatgcctaacctagggtaacttgttaccctaggtcattgcagtttccattaaatta Find is-nt database================================================ Query_seq: AA02465:AA02466|AA02465:AA02466:glucosamine-6-phosphate isomerase:N-acetylneuraminate lyase:->->:1704946..1705102 157 agtgaaattcctttattctatcgccgtgggtaataaattacccacgattatgtataattcagccccttttgggctgtataggttccccaaaggggaacaactatgcctaacctagggtaacttgttaccctaggtcattgcagtttccattaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02465:AA02466|AA02465:AA02466:glucosamine-6-phosphate isomerase:N-acetylneuraminate lyase:->->:1704946..1705102 157 agtgaaattcctttattctatcgccgtgggtaataaattacccacgattatgtataattcagccccttttgggctgtataggttccccaaaggggaacaactatgcctaacctagggtaacttgttaccctaggtcattgcagtttccattaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02465:AA02466|AA02465:AA02466:glucosamine-6-phosphate isomerase:N-acetylneuraminate lyase:->->:1704946..1705102 157 agtgaaattcctttattctatcgccgtgggtaataaattacccacgattatgtataattcagccccttttgggctgtataggttccccaaaggggaacaactatgcctaacctagggtaacttgttaccctaggtcattgcagtttccattaaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agtgaaattcctttattctatcgccgtgggtaataaattacccacgattatgtataattcagccccttttgggctgtataggttccccaaaggggaacaactatgcctaacctagggtaacttgttaccctaggtcattgcagtttccattaaatta Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 15 End: 107 .............. M L S P W V I N Y P R L C I I Q P L L G C I G S P K G N N Y A .................................................. Protein_Len: 37 Strand: + Start: 2 End: 112 . M K F L Y S I A V G N K L P T I M Y N S A P F G L Y R F P K G E Q L C L T ............................................. agtgaaattcctttattctatcgccgtgggtaataaattacccacgattatgtataattcagccccttttgggctgtataggttccccaaaggggaacaactatgcctaacctagggtaacttgttaccctaggtcattgcagtttccattaaatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agtgaaattcctttattctatcgccgtgggtaataaattacccacgattatgtataattcagccccttttgggctgtataggttccccaaaggggaacaactatgcctaacctagggtaacttgttaccctaggtcattgcagtttccattaaatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 55 HP_score: -6.8 Tail_Score: -2.52464 Start: 58 End: 78 Full_Region: CACGATTATGTATAA TTCAGCCC CTTTT GGGCTGTA TAGGTTCCCCAAAGG .........................................................TTCAGCCCCTTTTGGGCTGTA............................................................................... TransTerm Strand: - Conf: 61 HP_score: -6.8 Tail_Score: -3.38272 Start: 58 End: 78 Full_Region: cctttggggaaccta tacagccc aaaag gggctgaa ttatacataatcgtg .........................................................tacagcccaaaaggggctgaa............................................................................... TransTerm Strand: + Conf: 95 HP_score: -14.0 Tail_Score: -2.7728 Start: 111 End: 134 Full_Region: AACAACTATGCCTAA CCTAGGGTAA CTTG TTACCCTAGG TCATTGCAGTTTCCA ..............................................................................................................CCTAGGGTAACTTGTTACCCTAGG....................... Find igs database================================================ Query_seq: AA02465:AA02466|AA02465:AA02466:glucosamine-6-phosphate isomerase:N-acetylneuraminate lyase:->->:1704946..1705102 157 agtgaaattcctttattctatcgccgtgggtaataaattacccacgattatgtataattcagccccttttgggctgtataggttccccaaaggggaacaactatgcctaacctagggtaacttgttaccctaggtcattgcagtttccattaaatta Intra-Species Hit: Count: 1 Min: 1 Max: 157 Len: 157 Subject: aact_AA02465_AA02466|glucosamine-6-phosphate isomerase:N-acetylneuraminate lyase|POSITIVE:POSITIVE|[1704946,1705102]|157 HSP 1 e-value: 4.0E-84 bit: 311.0 Len: 157 Query Start:1 Query End:157 Subject Strand: POSITIVE Subject Start: 1 Subject End: 157 agtgaaattcctttattctatcgccgtgggtaataaattacccacgattatgtataattcagccccttttgggctgtataggttccccaaaggggaacaactatgcctaacctagggtaacttgttaccctaggtcattgcagtttccattaaatta agtgaaattcctttattctatcgccgtgggtaataaattacccacgattatgtataattcagccccttttgggctgtataggttccccaaaggggaacaactatgcctaacctagggtaacttgttaccctaggtcattgcagtttccattaaatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUGAAAUUCCUUUAUUCUAUCGCCGUGGGUAAUAAAUUACCCACGAUUAUGUAUAAUUCAGCCCCUUUUGGGCUGUAUAGGUUCCCCAAAGGGGAACAACUAUGCCUAACCUAGGGUAACUUGUUACCCUAGGUCAUUGCAGUUUCCAUUAAAUUA .(.((((((.(.............(((((((((....))))))))).....(((((...((((((.....)))))).....(((((((...)))))))...)))))...(((((((((((....)))))))))))....).)))))))......... (-58.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUAAUGGAAACUGCAAUGACCUAGGGUAACAAGUUACCCUAGGUUAGGCAUAGUUGUUCCCCUUUGGGGAACCUAUACAGCCCAAAAGGGGCUGAAUUAUACAUAAUCGUGGGUAAUUUAUUACCCACGGCGAUAGAAUAAAGGAAUUUCACU ........(((((..(((..(((((((((((((....))))))))))))).))).....(((((((...)))))))((((.((((((.....))))))............((((((((((....))))))))))..))))..........))))).. (-61.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 676 130 85 186 141 aact:AA00982|5end_aldo-keto_reductase_686526..686756_POSITIVE 646 129 81 165 117 aact:AA00702|5end_conserved_hypothetical_protein_481660..481890_POSITIVE 497 69 23 162 116 aact:AA00978|5end_conserved_hypothetical_protein_683399..683629_POSITIVE 416 130 85 211 168 aact:AA01354|5end_conserved_hypothetical_protein_916489..916719_POSITIVE 381 88 48 149 106 aact:AA01991|5end_conserved_hypothetical_protein_(possible_nitroreductase)_1352372..1352602_POSITIVE 314 135 92 123 78 aact:AA00918|5end_hypothetical_protein_630044..630274_POSITIVE 301 118 72 156 101 aact:AA00674|5end_nicotinate-nucleotide-dimethylbenzimidazole_phosphoribosyltransferase_461810..462040_POSITIVE 293 130 83 216 169 aact:AA02414|5end_hypothetical_protein_1658698..1658928_POSITIVE 287 99 54 88 44 aact:AA01252|5end_conserved_hypothetical_protein_(probable_SirA_homolog)_852023..852253_POSITIVE 287 110 61 195 123 aact:AA00056|5end_cytochrome_c-type_biogenesis_protein_43925..44155_POSITIVE 283 148 101 100 51 aact:AA00164|5end_dephospho-CoA_kinase_(dephosphocoenzyme_A_kinase)_116745..116975_POSITIVE 280 100 62 229 181 aact:AA02949|5end_hypothetical_protein_2061944..2062174_POSITIVE 277 130 86 186 142 aact:AA00276|5end_dethiobiotin_synthase_193232..193462_POSITIVE 271 97 62 227 182 aact:AA01379|5end_glycerophosphoryl_diester_phosphodiesterase_(protein_D)_933029..933259_POSITIVE 270 100 56 195 141 aact:AA02108|5end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1433393..1433623_POSITIVE 269 68 23 208 159 aact:AA02670|5end_hypothetical_protein_1858049..1858279_POSITIVE 269 118 72 160 110 aact:AA00545|5end_glutathionylspermidine_synthetase;amidase_373607..373837_POSITIVE 267 52 22 79 52 aact:AA01932|5end_tRNA_pseudouridine_synthase_B_1305098..1305328_POSITIVE 266 89 41 149 97 aact:AA01995|5end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355120..1355350_POSITIVE 266 135 91 187 147 aact:AA00983|5end_aldo-keto_reductase_687570..687800_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 444 106 61 57 9 aact:AA00977|3end_transcriptional_regulator_(LysR_family)_683334..683484_POSITIVE 375 113 72 70 29 aact:AA01342|3end_conserved_hypotheticl_protein_(possible_ribosomal_RNA_adenine_dimethylase)_911085..911235_POSITIVE 314 135 92 150 105 aact:AA00915|3end_xanthine-guanine_phosphoribosyltransferase_630151..630301_POSITIVE 302 120 71 53 4 aact:AA02465|3end_glucosamine-6-phosphate_isomerase_1704885..1705035_POSITIVE 301 98 52 151 101 aact:AA01566|3end_conserved_hypothetical_protein_1052091..1052241_POSITIVE 301 98 52 151 101 aact:AA01261|3end_conserved_hypothetical_protein_856723..856873_POSITIVE 300 106 62 93 31 aact:AA02947|3end_riboflavin_biosynthesis_protein_2061874..2062024_POSITIVE 300 56 18 31 2 aact:AA01842|3end_conserved_hypothetical_protein_1238467..1238617_POSITIVE 286 105 61 93 44 aact:AA00978|3end_conserved_hypothetical_protein_684585..684735_POSITIVE 284 107 62 96 53 aact:AA01614|3end_D-isomer_specific_2-hydroxyacid_dehydrogenase_family_protein_1090719..1090869_POSITIVE 283 63 22 58 13 aact:AA01351|3end_probable_sulfatase_916393..916543_POSITIVE 282 134 93 85 46 aact:AA01567|3end_conserved_hypothetical_protein_(possible_cell-wall-associated_hydrolase)_1055215..1055365_POSITIVE 278 148 104 47 2 aact:AA00163|3end_type_IV_prepilin_peptidase;_type_II_secretion_pathway_related_protein_116772..116922_POSITIVE 277 130 86 111 67 aact:AA00275|3end_conserved_hypothetical_protein_193237..193387_POSITIVE 269 135 95 60 22 aact:AA00861|3end_transcription_repair_coupling_factor_596150..596300_POSITIVE 267 52 22 76 49 aact:AA01930|3end_ribosome-binding_factor_A_1305175..1305325_POSITIVE 266 135 91 120 80 aact:AA00982|3end_aldo-keto_reductase_687583..687733_POSITIVE 265 66 21 118 72 aact:AA02048|3end_galactitol_utilization_operon_repressor_1395579..1395729_POSITIVE 260 107 68 92 39 aact:AA02695|3end_hydrogenase-3_component_G_1879418..1879568_POSITIVE 257 149 103 140 92 aact:AA01889|3end_conserved_hypothetical_protein_(possible_permease)_1274590..1274740_POSITIVE