Origin IGS: tagttgatcacataaaagtgcggtagattttttcgatgttttaaaaacacgtggaaaatctaccgcactttttttgtagtatgggatttatcccaccttgatagcgcacgttttacggaacgcaccgatagcctattgatatacaaaaactcaccgctccgccatcggaaggtttttaaaatggtgtgattcccaccctaccattgatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatcaatggtagggtgggaatcacaccattttaaaaaccttccgatggcggagcggtgagtttttgtatatcaataggctatcggtgcgttccgtaaaacgtgcgctatcaaggtgggataaatcccatactacaaaaaaagtgcggtagattttccacgtgtttttaaaacatcgaaaaaatctaccgcacttttatgtgatcaacta Mask Tandem Repeat Region ================================================ tagttgatcacataaaagtgcggtagattttttcgatgttttaaaaacacgtggaaaatctaccgcactttttttgtagtatgggatttatcccaccttgatagcgcacgttttacggaacgcaccgatagcctattgatatacaaaaactcaccgctccgccatcggaaggtttttaaaatggtgtgattcccaccctaccattgatta Find is-nt database================================================ Query_seq: AA02462:AA02463|AA02462:AA02463:thiol peroxidase (scavengase):N-acetylglucosamine-6-phosphate deacetylase:->->:1702734..1702943 210 tagttgatcacataaaagtgcggtagattttttcgatgttttaaaaacacgtggaaaatctaccgcactttttttgtagtatgggatttatcccaccttgatagcgcacgttttacggaacgcaccgatagcctattgatatacaaaaactcaccgctccgccatcggaaggtttttaaaatggtgtgattcccaccctaccattgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02462:AA02463|AA02462:AA02463:thiol peroxidase (scavengase):N-acetylglucosamine-6-phosphate deacetylase:->->:1702734..1702943 210 tagttgatcacataaaagtgcggtagattttttcgatgttttaaaaacacgtggaaaatctaccgcactttttttgtagtatgggatttatcccaccttgatagcgcacgttttacggaacgcaccgatagcctattgatatacaaaaactcaccgctccgccatcggaaggtttttaaaatggtgtgattcccaccctaccattgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02462:AA02463|AA02462:AA02463:thiol peroxidase (scavengase):N-acetylglucosamine-6-phosphate deacetylase:->->:1702734..1702943 210 tagttgatcacataaaagtgcggtagattttttcgatgttttaaaaacacgtggaaaatctaccgcactttttttgtagtatgggatttatcccaccttgatagcgcacgttttacggaacgcaccgatagcctattgatatacaaaaactcaccgctccgccatcggaaggtttttaaaatggtgtgattcccaccctaccattgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagttgatcacataaaagtgcggtagattttttcgatgttttaaaaacacgtggaaaatctaccgcactttttttgtagtatgggatttatcccaccttgatagcgcacgttttacggaacgcaccgatagcctattgatatacaaaaactcaccgctccgccatcggaaggtttttaaaatggtgtgattcccaccctaccattgatta Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 58 End: 186 ......................................................... M Y R T F F V V W D L S H L D S A R F T E R T D S L L I Y K N S P L R H R K V F K M V ........................ Protein_Len: 40 Strand: - Start: 63 End: 182 .............................................................. R V K K T T H S K D W R S L A R K V S R V S L R N I Y L F E G S R W R F T K L M ............................ tagttgatcacataaaagtgcggtagattttttcgatgttttaaaaacacgtggaaaatctaccgcactttttttgtagtatgggatttatcccaccttgatagcgcacgttttacggaacgcaccgatagcctattgatatacaaaaactcaccgctccgccatcggaaggtttttaaaatggtgtgattcccaccctaccattgatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagttgatcacataaaagtgcggtagattttttcgatgttttaaaaacacgtggaaaatctaccgcactttttttgtagtatgggatttatcccaccttgatagcgcacgttttacggaacgcaccgatagcctattgatatacaaaaactcaccgctccgccatcggaaggtttttaaaatggtgtgattcccaccctaccattgatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -15.9 Tail_Score: -5.69473 Start: 18 End: 68 Full_Region: GTTGATCACATAAAA GTGCGGTAGATTTTTTCGATGTTT TAA AAACACGTGGAAAATCTACCGCAC TTTTTTTGTAGTATG .................GTGCGGTAGATTTTTTCGATGTTTTAAAAACACGTGGAAAATCTACCGCAC.............................................................................................................................................. TransTerm Strand: - Conf: 93 HP_score: -11.5 Tail_Score: -4.57353 Start: 18 End: 68 Full_Region: catactacaaaaaaa gtgcggtagattttccacgtgttt tta aaacatcgaaaaaatctaccgcac ttttatgtgatcaac .................gtgcggtagattttccacgtgtttttaaaacatcgaaaaaatctaccgcac.............................................................................................................................................. Find igs database================================================ Query_seq: AA02462:AA02463|AA02462:AA02463:thiol peroxidase (scavengase):N-acetylglucosamine-6-phosphate deacetylase:->->:1702734..1702943 210 tagttgatcacataaaagtgcggtagattttttcgatgttttaaaaacacgtggaaaatctaccgcactttttttgtagtatgggatttatcccaccttgatagcgcacgttttacggaacgcaccgatagcctattgatatacaaaaactcaccgctccgccatcggaaggtttttaaaatggtgtgattcccaccctaccattgatta Intra-Species Hit: Count: 1 Min: 1 Max: 210 Len: 210 Subject: aact_AA02462_AA02463|thiol peroxidase (scavengase):N-acetylglucosamine-6-phosphate deacetylase|POSITIVE:POSITIVE|[1702734,1702943]|210 HSP 1 e-value: 1.0E-103 bit: 375.0 Len: 210 Query Start:1 Query End:210 Subject Strand: POSITIVE Subject Start: 1 Subject End: 210 tagttgatcacataaaagtgcggtagattttttcgatgttttaaaaacacgtggaaaatctaccgcacnnnnnnngtagtatgggatttatcccaccttgatagcgcacgttttacggaacgcaccgatagcctattgatatacaaaaactcaccgctccgccatcggaaggtttttaaaatggtgtgattcccaccctaccattgatta tagttgatcacataaaagtgcggtagattttttcgatgttttaaaaacacgtggaaaatctaccgcactttttttgtagtatgggatttatcccaccttgatagcgcacgttttacggaacgcaccgatagcctattgatatacaaaaactcaccgctccgccatcggaaggtttttaaaatggtgtgattcccaccctaccattgatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUUGAUCACAUAAAAGUGCGGUAGAUUUUUUCGAUGUUUUAAAAACACGUGGAAAAUCUACCGCACUUUUUUUGUAGUAUGGGAUUUAUCCCACCUUGAUAGCGCACGUUUUACGGAACGCACCGAUAGCCUAUUGAUAUACAAAAACUCACCGCUCCGCCAUCGGAAGGUUUUUAAAAUGGUGUGAUUCCCACCCUACCAUUGAUUA ....((((((...((((((((((((((((((((((.((((.....)))))).))))))))))))))))))))...((((..(((((.(((..(((..(..((((.((.(((((....)))))........))))))..).............(((..((((....)))).)))........)))..))).)))))..))))...)))))) (-66.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCAAUGGUAGGGUGGGAAUCACACCAUUUUAAAAACCUUCCGAUGGCGGAGCGGUGAGUUUUUGUAUAUCAAUAGGCUAUCGGUGCGUUCCGUAAAACGUGCGCUAUCAAGGUGGGAUAAAUCCCAUACUACAAAAAAAGUGCGGUAGAUUUUCCACGUGUUUUUAAAACAUCGAAAAAAUCUACCGCACUUUUAUGUGAUCAACUA ..((((...((((..((((((((.((((..............((((((((.((((.....))))(((.....)))...)))))))).((((..((.....)).))))......)))).)))...)))))..))))...((((((((((((((((((...((((((.....)))).))..))))))))))))))))))...))))...... (-71.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 439 78 31 203 158 aact:AA01471|5end_type_I_restriction_enzyme_M_(modification_chain)_987321..987551_POSITIVE 436 70 21 168 109 aact:AA01918|5end_anaerobic_C4-dicarboxylate_transporter_1290931..1291161_POSITIVE 424 70 21 182 132 aact:AA01824|5end_iron(III)_ABC_transporter,_ATP-binding_protein_1227705..1227935_POSITIVE 406 70 21 172 123 aact:AA01666|5end_penicillin-binding_protein_4;_D-alanyl-D-alanine_carboxypeptidase;_D-alanyl-D-alanine-endopeptidase_1128231..1128461_POSITIVE 404 204 162 183 131 aact:AA00979|5end_type_B_carboxylesterase;_p-nitrobenzyl_esterase_684645..684875_POSITIVE 401 70 21 167 118 aact:AA02597|5end_ribonuclease_D_1807124..1807354_POSITIVE 401 85 41 52 11 aact:AA01823|5end_hypothetical_protein_1227563..1227793_POSITIVE 398 97 52 229 184 aact:AA00296|5end_conserved_hypothetical_protein_(possible_hemolysin_/_transport_protein)_202384..202614_POSITIVE 398 97 52 112 67 aact:AA00295|5end_hypothetical_protein_202267..202497_POSITIVE 394 72 33 169 121 aact:AA01458|5end_aminotransferase_978820..979050_POSITIVE 393 78 35 128 88 aact:AA01390|5end_LexA_repressor_937059..937289_POSITIVE 391 42 9 160 126 aact:AA01220|5end_competence_protein_F;_amidophosphoribosyltransferase_832775..833005_POSITIVE 391 42 9 41 7 aact:AA01217|5end_hypothetical_protein_832656..832886_POSITIVE 373 60 13 152 92 aact:AA00186|5end_o-succinylbenzoate-CoA_synthase;_OSB_synthase_131367..131597_POSITIVE 367 86 49 126 86 aact:AA01728|5end_conserved_hypothetical_protein(related_to_HipA_protein)_1168337..1168567_POSITIVE 365 70 21 175 126 aact:AA01564|5end_transaldolase_B_1049099..1049329_POSITIVE 363 70 21 163 117 aact:AA02249|5end_thiamine_biosynthesis_protein_1528365..1528595_POSITIVE 361 89 43 62 11 aact:AA01041|5end_hypothetical_protein_724853..725083_POSITIVE 355 90 43 188 139 aact:AA02706|5end_2-oxoglutarate_dehydrogenase_E1_component_(decarboxylase_component)_1886843..1887073_POSITIVE 355 90 43 62 13 aact:AA02705|5end_hypothetical_protein_1886717..1886947_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 456 70 21 75 26 aact:AA02462|3end_thiol_peroxidase_(scavengase)_1702673..1702823_POSITIVE 424 70 21 144 94 aact:AA01823|3end_hypothetical_protein_1227747..1227897_POSITIVE 424 70 21 68 18 aact:AA01822|3end_lysostaphin_precursor_1227671..1227821_POSITIVE 408 68 21 151 94 aact:AA01917|3end_hypothetical_protein_1290996..1291146_POSITIVE 406 70 21 65 16 aact:AA01663|3end_GTP_cyclohydrolase_I_1128204..1128354_POSITIVE 401 70 21 73 24 aact:AA02595|3end_cytochrome_c_peroxidase_1807110..1807260_POSITIVE 398 97 52 103 58 aact:AA00292|3end_dihydrofolate_reductase_202338..202488_POSITIVE 394 72 33 77 29 aact:AA01457|3end_D-xylose_ABC_transporter,_membrane_spanning_protein_978808..978958_POSITIVE 393 78 35 147 107 aact:AA01389|3end_hypothetical_protein_937158..937308_POSITIVE 373 89 49 104 63 aact:AA02697|3end_hydrogenase_3_maturation_protease_1880408..1880558_POSITIVE 367 86 49 129 89 aact:AA01727|3end_conserved_hypothetical_protein_(possible_HipA_protein)_1168420..1168570_POSITIVE 363 70 21 73 27 aact:AA02246|3end_peptidase_E_1528355..1528505_POSITIVE 361 89 43 75 24 aact:AA01040|3end_possible_lipoprotein_724946..725096_POSITIVE 360 70 21 60 5 aact:AA01945|3end_selenophosphate_synthase_1316022..1316172_POSITIVE 355 90 43 60 11 aact:AA02704|3end_dihydrolipoamide_succinyltransferase_1886795..1886945_POSITIVE 354 73 33 89 55 aact:AA00513|3end_pseudouridylate_synthase_(pseudouridine_synthase)_355686..355836_POSITIVE 351 75 31 76 33 aact:AA02446|3end_conserved_hypothetical_protein_1687583..1687733_POSITIVE 349 77 31 91 47 aact:AA02940|3end_conserved_hypothetical_protein_2058652..2058802_POSITIVE 342 70 21 70 20 aact:AA01057|3end_DnaJ-like_protein_djlA_735047..735197_POSITIVE 340 204 169 43 2 aact:AA00978|3end_conserved_hypothetical_protein_684585..684735_POSITIVE