Origin IGS: cgctcacctcactctctgttaataaggtgattttacctttttctcaggtttcgaacagctatggtcgggagacgctcgccatcgtggggggataatatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatattatccccccacgatggcgagcgtctcccgaccatagctgttcgaaacctgagaaaaaggtaaaatcaccttattaacagagagtgaggtgagcg Mask Tandem Repeat Region ================================================ cgctcacctcactctctgttaataaggtgattttacctttttctcaggtttcgaacagctatggtcgggagacgctcgccatcgtggggggataatatta Find is-nt database================================================ Query_seq: AA02451:AA02453|AA02451:AA02453:conserved hypothetical protein:conserved hypothetical protein:->->:1688987..1689086 100 cgctcacctcactctctgttaataaggtgattttacctttttctcaggtttcgaacagctatggtcgggagacgctcgccatcgtggggggataatatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02451:AA02453|AA02451:AA02453:conserved hypothetical protein:conserved hypothetical protein:->->:1688987..1689086 100 cgctcacctcactctctgttaataaggtgattttacctttttctcaggtttcgaacagctatggtcgggagacgctcgccatcgtggggggataatatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02451:AA02453|AA02451:AA02453:conserved hypothetical protein:conserved hypothetical protein:->->:1688987..1689086 100 cgctcacctcactctctgttaataaggtgattttacctttttctcaggtttcgaacagctatggtcgggagacgctcgccatcgtggggggataatatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cgctcacctcactctctgttaataaggtgattttacctttttctcaggtttcgaacagctatggtcgggagacgctcgccatcgtggggggataatatta Predict ORF larger than 30AA ================================================ cgctcacctcactctctgttaataaggtgattttacctttttctcaggtttcgaacagctatggtcgggagacgctcgccatcgtggggggataatatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cgctcacctcactctctgttaataaggtgattttacctttttctcaggtttcgaacagctatggtcgggagacgctcgccatcgtggggggataatatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02451:AA02453|AA02451:AA02453:conserved hypothetical protein:conserved hypothetical protein:->->:1688987..1689086 100 cgctcacctcactctctgttaataaggtgattttacctttttctcaggtttcgaacagctatggtcgggagacgctcgccatcgtggggggataatatta Intra-Species Hit: Count: 1 Min: 1 Max: 100 Len: 100 Subject: aact_AA02451_AA02453|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1688987,1689086]|100 HSP 1 e-value: 2.0E-50 bit: 198.0 Len: 100 Query Start:1 Query End:100 Subject Strand: POSITIVE Subject Start: 1 Subject End: 100 cgctcacctcactctctgttaataaggtgattttacctttttctcaggtttcgaacagctatggtcgggagacgctcgccatcgtggggggataatatta cgctcacctcactctctgttaataaggtgattttacctttttctcaggtttcgaacagctatggtcgggagacgctcgccatcgtggggggataatatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGCUCACCUCACUCUCUGUUAAUAAGGUGAUUUUACCUUUUUCUCAGGUUUCGAACAGCUAUGGUCGGGAGACGCUCGCCAUCGUGGGGGGAUAAUAUUA ...((.((((((..((((..((.((((((....)))))).))..))))............((((.((((.....)))))))).)))))).))........ (-26.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAUUAUCCCCCCACGAUGGCGAGCGUCUCCCGACCAUAGCUGUUCGAAACCUGAGAAAAAGGUAAAAUCACCUUAUUAACAGAGAGUGAGGUGAGCG ................(((((((....(((....)))....)))).)))..((((.......))))....((((((((((.......))))))))))... (-23.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 561 88 48 229 189 aact:AA00474|5end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_330175..330405_POSITIVE 549 85 49 173 137 aact:AA00482|5end_hydrolase_(haloacid_dehalogenase-like)_(HAD_superfamily)_335232..335462_POSITIVE 419 89 41 89 33 aact:AA01741|5end_6-pyruvoyl_tetrahydrobiopterin_synthase_1177193..1177423_POSITIVE 375 89 44 189 131 aact:AA01853|5end_hypothetical_protein_1246458..1246688_POSITIVE 360 86 47 60 14 aact:AA01270|5end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863317..863547_POSITIVE 354 89 41 97 51 aact:AA00761|5end_conserved_hypothetical_protein_(possible_peptidase)_523554..523784_POSITIVE 344 90 49 161 123 aact:AA00299|5end_hypothetical_protein_204256..204486_POSITIVE 342 91 56 228 194 aact:AA00516|5end_autoinducer-2_production_protein_355931..356161_POSITIVE 341 87 44 165 117 aact:AA02972|5end_conserved_hypothetical_protein_(tyrosine_phenol_lyase-related_protein)_2077381..2077611_POSITIVE 341 95 51 57 4 aact:AA00613|5end_lipid-A-disaccharide_synthase_418713..418943_POSITIVE 333 86 51 55 15 aact:AA02321|5end_conserved_hypothetical_protein_1585163..1585393_POSITIVE 333 81 44 93 49 aact:AA00602|5end_1-deoxy-D-xylulose_5-phosphate_reductoisomerase_409077..409307_POSITIVE 332 89 51 96 54 aact:AA01515|5end_SlyX_family_protein_1020682..1020912_POSITIVE 331 92 51 99 48 aact:AA02470|5end_glucose_kinase_1706730..1706960_POSITIVE 326 90 48 55 10 aact:AA02885|5end_conserved_hypothetical_protein_2022428..2022658_POSITIVE 321 94 55 164 120 aact:AA01770|5end_hypothetical_protein_1193111..1193341_POSITIVE 318 86 52 139 104 aact:AA02371|5end_oxygen-independent_coproporphyrinogen_III_oxidase_1624580..1624810_POSITIVE 318 90 46 199 167 aact:AA00199|5end_hypothetical_protein_138575..138805_POSITIVE 317 69 22 231 188 aact:AA01441|5end_tRNA/rRNA_methylase_966490..966720_POSITIVE 317 90 43 181 124 aact:AA00500|5end_hypothetical_protein_347151..347381_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 561 88 48 51 11 aact:AA00473|3end_tryptophanyl-tRNA_synthetase_330077..330227_POSITIVE 549 85 49 60 24 aact:AA00480|3end_mannose-specific_phosphotransferase_system_IID_335199..335349_POSITIVE 518 90 48 68 27 aact:AA01173|3end_VapD-homolog_804385..804535_POSITIVE 496 85 49 48 11 aact:AA00776|3end_conserved_hypothetical_protein_533869..534019_POSITIVE 423 86 42 110 68 aact:AA01660|3end_hypothetical_protein_1125282..1125432_POSITIVE 419 89 41 59 3 aact:AA01740|3end_conserved_hypothetical_protein_1177243..1177393_POSITIVE 385 90 50 146 96 aact:AA01510|3end_ABC_transporter,_ATP-binding_protein/permease_1018165..1018315_POSITIVE 379 88 48 51 10 aact:AA02954|3end_penicillin_tolerance_protein;_hydroxymethylbutenyl_pyrophosphate_reductase_2066631..2066781_POSITIVE 360 86 47 76 30 aact:AA01269|3end_hypothetical_protein_863413..863563_POSITIVE 342 91 56 55 21 aact:AA00514|3end_hypothetical_protein_355838..355988_POSITIVE 341 87 44 95 47 aact:AA02970|3end_tyrosine_phenol-lyase_2077391..2077541_POSITIVE 341 89 46 144 82 aact:AA02911|3end_hypothetical_protein_2039235..2039385_POSITIVE 333 86 51 46 6 aact:AA02320|3end_ABC_transporter_ATP-binding_protein_1585234..1585384_POSITIVE 333 81 44 55 11 aact:AA00601|3end_hypothetical_protein_409119..409269_POSITIVE 331 92 51 86 35 aact:AA02468|3end_conserved_hypothetical_protein_1706797..1706947_POSITIVE 328 99 57 102 60 aact:AA01764|3end_hypothetical_protein_1191698..1191848_POSITIVE 321 94 55 105 61 aact:AA01769|3end_hypothetical_protein_1193132..1193282_POSITIVE 318 86 52 127 92 aact:AA02369|3end_conserved_hypothetical_protein_1624648..1624798_POSITIVE 313 88 46 104 63 aact:AA00927|3end_ATP-dependent_DNA_helicase_637777..637927_POSITIVE 313 91 55 141 103 aact:AA00007|3end_superoxide_dismutase_[Cu-Zn]_precursor_5055..5205_POSITIVE