Origin IGS: taatggaaaagtgcggtcggttttggaaatgttttttgagaacaaaatgaaggttcaaacatccaatgcttaaacctgatcagaacaagctgtgaatgcttgcgctatgccaaaacagcaggggcaggtcctgtgcctgcccgaatttttcgatgaatccaacgggcgggcacaggacccgcccctacgcgaaaacacaaaaaaacacctttgatagcgcacgcttcatagcgcgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcaccagcaaaatgtaacgtttttcaaaattcaactgtcataaggaaactct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agagtttccttatgacagttgaattttgaaaaacgttacattttgctggtgcgcgtttcctaacgcgtgcctgtaatatcaataagttaaaagcacgcgctatgaagcgtgcgctatcaaaggtgtttttttgtgttttcgcgtaggggcgggtcctgtgcccgcccgttggattcatcgaaaaattcgggcaggcacaggacctgcccctgctgttttggcatagcgcaagcattcacagcttgttctgatcaggtttaagcattggatgtttgaaccttcattttgttctcaaaaaacatttccaaaaccgaccgcacttttccatta Mask Tandem Repeat Region ================================================ taatggaaaagtgcggtcggttttggaaatgttttttgagaacaaaatgaaggttcaaacatccaatgcttaaacctgatcagaacaagctgtgaatgcttgcgctatgccaaaacagcaggggcaggtcctgtgcctgcccgaatttttcgatgaatccaacgggcgggcacaggacccgcccctacgcgaaaacacaaaaaaacacctttgatagcgcacgcttcatagcgcgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcaccagcaaaatgtaacgtttttcaaaattcaactgtcataaggaaactct Find is-nt database================================================ Query_seq: AA02443:AA02446|AA02443:AA02446:cytochrome C related protein:conserved hypothetical protein:->->:1686651..1686980 330 taatggaaaagtgcggtcggttttggaaatgttttttgagaacaaaatgaaggttcaaacatccaatgcttaaacctgatcagaacaagctgtgaatgcttgcgctatgccaaaacagcaggggcaggtcctgtgcctgcccgaatttttcgatgaatccaacgggcgggcacaggacccgcccctacgcgaaaacacaaaaaaacacctttgatagcgcacgcttcatagcgcgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcaccagcaaaatgtaacgtttttcaaaattcaactgtcataaggaaactct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02443:AA02446|AA02443:AA02446:cytochrome C related protein:conserved hypothetical protein:->->:1686651..1686980 330 taatggaaaagtgcggtcggttttggaaatgttttttgagaacaaaatgaaggttcaaacatccaatgcttaaacctgatcagaacaagctgtgaatgcttgcgctatgccaaaacagcaggggcaggtcctgtgcctgcccgaatttttcgatgaatccaacgggcgggcacaggacccgcccctacgcgaaaacacaaaaaaacacctttgatagcgcacgcttcatagcgcgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcaccagcaaaatgtaacgtttttcaaaattcaactgtcataaggaaactct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02443:AA02446|AA02443:AA02446:cytochrome C related protein:conserved hypothetical protein:->->:1686651..1686980 330 taatggaaaagtgcggtcggttttggaaatgttttttgagaacaaaatgaaggttcaaacatccaatgcttaaacctgatcagaacaagctgtgaatgcttgcgctatgccaaaacagcaggggcaggtcctgtgcctgcccgaatttttcgatgaatccaacgggcgggcacaggacccgcccctacgcgaaaacacaaaaaaacacctttgatagcgcacgcttcatagcgcgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcaccagcaaaatgtaacgtttttcaaaattcaactgtcataaggaaactct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatggaaaagtgcggtcggttttggaaatgttttttgagaacaaaatgaaggttcaaacatccaatgcttaaacctgatcagaacaagctgtgaatgcttgcgctatgccaaaacagcaggggcaggtcctgtgcctgcccgaatttttcgatgaatccaacgggcgggcacaggacccgcccctacgcgaaaacacaaaaaaacacctttgatagcgcacgcttcatagcgcgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcaccagcaaaatgtaacgtttttcaaaattcaactgtcataaggaaactct Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 61 End: 153 ............................................................ M Q C L N L I R T S C E C L R Y A K T A G A G P V P A R I F R ................................................................................................................................................................................. Protein_Len: 40 Strand: + Start: 92 End: 211 ........................................................................................... M N A C A M P K Q Q G Q V L C L P E F F D E S N G R A Q D P P L R E N T K K H L ....................................................................................................................... Protein_Len: 60 Strand: + Start: 96 End: 329 ............................................................................................... M L A L C Q N S R G R S C A C P N F S M N P T G G H R T R P Y A K T Q K N T F D S A R F I A R A F N L L I L Q A R V R K . Protein_Len: 36 Strand: - Start: 108 End: 215 ........................................................................................................... A L V A P A P G T G A R I K R H I W R A P V P G A G V R F C L F V G K M ................................................................................................................... Protein_Len: 31 Strand: - Start: 83 End: 175 .................................................................................. F L S H I S A S H W F L L P L D Q A Q G F K E I F G V P P C M ........................................................................................................................................................... Protein_Len: 50 Strand: - Start: 73 End: 222 ........................................................................ V Q D S C A T F A Q A I G F C C P C T R H R G S N K S S D L P R A C S G G R R S F V F F C R Q Y R M ............................................................................................................ taatggaaaagtgcggtcggttttggaaatgttttttgagaacaaaatgaaggttcaaacatccaatgcttaaacctgatcagaacaagctgtgaatgcttgcgctatgccaaaacagcaggggcaggtcctgtgcctgcccgaatttttcgatgaatccaacgggcgggcacaggacccgcccctacgcgaaaacacaaaaaaacacctttgatagcgcacgcttcatagcgcgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcaccagcaaaatgtaacgtttttcaaaattcaactgtcataaggaaactct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatggaaaagtgcggtcggttttggaaatgttttttgagaacaaaatgaaggttcaaacatccaatgcttaaacctgatcagaacaagctgtgaatgcttgcgctatgccaaaacagcaggggcaggtcctgtgcctgcccgaatttttcgatgaatccaacgggcgggcacaggacccgcccctacgcgaaaacacaaaaaaacacctttgatagcgcacgcttcatagcgcgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcaccagcaaaatgtaacgtttttcaaaattcaactgtcataaggaaactct Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 74 HP_score: -8.7 Tail_Score: -3.85542 Start: 52 End: 76 Full_Region: agcttgttctgatca ggtttaagcat tgg atgtttgaacc ttcattttgttctca ...................................................ggtttaagcattggatgtttgaacc.............................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 49 HP_score: -3.7 Tail_Score: -3.43288 Start: 86 End: 102 Full_Region: tgttttggcatagcg caagc attcaca gcttg ttctgatcaggttta .....................................................................................caagcattcacagcttg.................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 76 HP_score: -7.9 Tail_Score: -4.36729 Start: 217 End: 238 Full_Region: AAAACACCTTTGATA GCGCACGCT TCAT AGCGCGTGC TTTTAACTTATTGAT ........................................................................................................................................................................................................................GCGCACGCTTCATAGCGCGTGC............................................................................................ TransTerm Strand: - Conf: 74 HP_score: -11.0 Tail_Score: -2.8655 Start: 260 End: 283 Full_Region: acgttacattttgct ggtgcgcgtt tcct aacgcgtgcc tgtaatatcaataag ...................................................................................................................................................................................................................................................................ggtgcgcgtttcctaacgcgtgcc............................................... Find igs database================================================ Query_seq: AA02443:AA02446|AA02443:AA02446:cytochrome C related protein:conserved hypothetical protein:->->:1686651..1686980 330 taatggaaaagtgcggtcggttttggaaatgttttttgagaacaaaatgaaggttcaaacatccaatgcttaaacctgatcagaacaagctgtgaatgcttgcgctatgccaaaacagcaggggcaggtcctgtgcctgcccgaatttttcgatgaatccaacgggcgggcacaggacccgcccctacgcgaaaacacaaaaaaacacctttgatagcgcacgcttcatagcgcgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcaccagcaaaatgtaacgtttttcaaaattcaactgtcataaggaaactct Intra-Species Hit: Count: 1 Min: 1 Max: 330 Len: 330 Subject: aact_AA02443_AA02446|cytochrome C related protein:conserved hypothetical protein|POSITIVE:POSITIVE|[1686651,1686980]|330 HSP 1 e-value: 1.0E-174 bit: 613.0 Len: 330 Query Start:1 Query End:330 Subject Strand: POSITIVE Subject Start: 1 Subject End: 330 taatggaaaagtgcggtcggttttggaaatgttttttgagaacaaaatgaaggttcaaacatccaatgcttaaacctgatcagaacaagctgtgaatgcttgcgctatgccaaaacagcaggggcaggtcctgtgcctgcccgaatttttcgatgaatccaacgggcgggcacaggacccgcccctacgcgaaaacacnnnnnnncacctttgatagcgcacgcttcatagcgcgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcaccagcaaaatgtaacgtttttcaaaattcaactgtcataaggaaactct taatggaaaagtgcggtcggttttggaaatgttttttgagaacaaaatgaaggttcaaacatccaatgcttaaacctgatcagaacaagctgtgaatgcttgcgctatgccaaaacagcaggggcaggtcctgtgcctgcccgaatttttcgatgaatccaacgggcgggcacaggacccgcccctacgcgaaaacacaaaaaaacacctttgatagcgcacgcttcatagcgcgtgcttttaacttattgatattacaggcacgcgttaggaaacgcgcaccagcaaaatgtaacgtttttcaaaattcaactgtcataaggaaactct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGGAAAAGUGCGGUCGGUUUUGGAAAUGUUUUUUGAGAACAAAAUGAAGGUUCAAACAUCCAAUGCUUAAACCUGAUCAGAACAAGCUGUGAAUGCUUGCGCUAUGCCAAAACAGCAGGGGCAGGUCCUGUGCCUGCCCGAAUUUUUCGAUGAAUCCAACGGGCGGGCACAGGACCCGCCCCUACGCGAAAACACAAAAAAACACCUUUGAUAGCGCACGCUUCAUAGCGCGUGCUUUUAACUUAUUGAUAUUACAGGCACGCGUUAGGAAACGCGCACCAGCAAAAUGUAACGUUUUUCAAAAUUCAACUGUCAUAAGGAAACUCU ......(((((..((....(((((((...(((((.....)))))......(((((.((.(((...))).)).)))))..)))))))..((((((((.((.((((((((.........((((((((.(((((((((((((((((.....(((...))).....))))))))))))))))).))))))..)).......((((........)))))))))))).)))))))))).))..)))))..(((((.((((.....((..((((((....))))))..)).(.((((((...)))))).)..........)))))))))........ (-119.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAGUUUCCUUAUGACAGUUGAAUUUUGAAAAACGUUACAUUUUGCUGGUGCGCGUUUCCUAACGCGUGCCUGUAAUAUCAAUAAGUUAAAAGCACGCGCUAUGAAGCGUGCGCUAUCAAAGGUGUUUUUUUGUGUUUUCGCGUAGGGGCGGGUCCUGUGCCCGCCCGUUGGAUUCAUCGAAAAAUUCGGGCAGGCACAGGACCUGCCCCUGCUGUUUUGGCAUAGCGCAAGCAUUCACAGCUUGUUCUGAUCAGGUUUAAGCAUUGGAUGUUUGAACCUUCAUUUUGUUCUCAAAAAACAUUUCCAAAACCGACCGCACUUUUCCAUUA ................(((.(((....((((((((.....(((((.(((((((((((((.((.(((((((...((((........))))...))))))).)).))))))))))))).))))).)))))))).((((..(((.((((((((((((((((((((.(((((.....(((...))).....))))).)))))))))))))))))))).(((((((..(((.((((((.......)))))).)))...((((((((((((...))))))))))))...(((((....))))).......))))))))))..))))..))).))). (-134.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 709 189 141 205 166 aact:AA02696|5end_formate_hydrogenlyase_maturation_protein_1879476..1879706_POSITIVE 427 171 131 129 73 aact:AA00551|5end_hypothetical_protein_377225..377455_POSITIVE 427 37 1 128 92 aact:AA00200|5end_thymidylate_synthase_138892..139122_POSITIVE 423 246 209 159 122 aact:AA01982|5end_maltodextrin_phosphorylase_1339387..1339617_POSITIVE 421 180 131 201 148 aact:AA01025|5end_hypothetical_protein_717522..717752_POSITIVE 419 297 257 121 81 aact:AA01432|5end_lysophospholipase_L2_960715..960945_POSITIVE 416 170 121 152 106 aact:AA01635|5end_ribonucleoside_diphosphate_reductase,_beta_chain_1104615..1104845_POSITIVE 416 279 231 81 31 aact:AA00738|5end_hypothetical_protein_505087..505317_POSITIVE 409 289 260 136 107 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 409 177 132 63 5 aact:AA00075|5end_conserved_hypothetical_protein_54963..55193_POSITIVE 401 43 3 192 149 aact:AA02261|5end_30S_ribosomal_protein_S9_1538013..1538243_POSITIVE 393 139 100 231 196 aact:AA01681|5end_molybdate-binding_periplasmic_protein_1135159..1135389_POSITIVE 393 130 82 102 46 aact:AA01654|5end_periplasmic_maltose-binding_protein_precursor_1119896..1120126_POSITIVE 393 200 151 93 31 aact:AA00634|5end_DNA_polymerase_IV_(Pol_IV)_435182..435412_POSITIVE 391 50 4 155 116 aact:AA01475|5end_conserved_hypothetical_protein_(possible_cytoplasmic_protein;_DNA_binding_protein)_990364..990594_POSITIVE 391 50 4 50 11 aact:AA01474|5end_hypothetical_protein_990259..990489_POSITIVE 387 300 260 182 152 aact:AA02212|5end_ribonucleoside-diphosphate_reductase_beta_chain_1506012..1506242_POSITIVE 384 41 2 139 100 aact:AA00386|5end_aspartate--ammonia_ligase_(asparagine_synthetase_A)_261150..261380_POSITIVE 382 139 91 185 122 aact:AA02736|5end_conserved_hypothetical_protein_1917350..1917580_POSITIVE 380 49 5 224 181 aact:AA02774|5end_collagenase_prtC_homolog,_possible_protease_1936870..1937100_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 709 189 141 67 28 aact:AA02695|3end_hydrogenase-3_component_G_1879418..1879568_POSITIVE 423 246 209 46 9 aact:AA01981|3end_4-alpha-glucanotransferase_1339354..1339504_POSITIVE 419 297 257 77 37 aact:AA01431|3end_conserved_hypothetical_protein_(possible_hydrolase)_960751..960901_POSITIVE 416 170 121 127 81 aact:AA01634|3end_hypothetical_protein_1104670..1104820_POSITIVE 416 279 231 57 7 aact:AA00737|3end_citrate_lyase,_alpha_chain_(citrase)_505143..505293_POSITIVE 409 289 260 53 24 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 401 43 3 61 18 aact:AA02260|3end_pseudouridylate_synthase_I_1537962..1538112_POSITIVE 393 130 82 130 74 aact:AA01651|3end_hypothetical_protein_1120004..1120154_POSITIVE 391 50 4 142 103 aact:AA01474|3end_hypothetical_protein_990431..990581_POSITIVE 387 300 260 46 16 aact:AA02210|3end_ribonucleoside-diphosphate_reductase_alpha_chain_1505956..1506106_POSITIVE 384 41 2 77 38 aact:AA00384|3end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_261168..261318_POSITIVE 380 49 5 50 7 aact:AA02772|3end_hypothetical_protein_1936776..1936926_POSITIVE 380 37 2 87 52 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 377 41 4 70 32 aact:AA02777|3end_tRNA-guanine_transglycosylase_1942527..1942677_POSITIVE 373 280 231 56 1 aact:AA01169|3end_hypothetical_protein_802229..802379_POSITIVE 366 191 153 66 38 aact:AA02952|3end_lipoprotein_signal_peptidase_2065687..2065837_POSITIVE 366 176 131 148 110 aact:AA02950|3end_isoleucyl-tRNA_synthetase_2065113..2065263_POSITIVE 366 187 150 149 107 aact:AA02005|3end_vancomycin_sensitivity;_SanA_protein_1364020..1364170_POSITIVE 362 142 101 136 89 aact:AA02477|3end_PTS_system,_N-acetylglucosamine-permease_IIABC_component_1713371..1713521_POSITIVE 359 165 121 125 71 aact:AA02103|3end_D-alanyl-D-alanine-endopeptidase;_penicillin-binding_protein_7_1431784..1431934_POSITIVE