Origin IGS: taaacctctgacttcaaatttgtaacaaatgctacatggatcacatttcaatgaaaaggaggttgattttcccttgcactctgaatttattagaggataattattatcaattacatcaagcaggaatagttttacgataaggagatgggat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atcccatctccttatcgtaaaactattcctgcttgatgtaattgataataattatcctctaataaattcagagtgcaagggaaaatcaacctccttttcattgaaatgtgatccatgtagcatttgttacaaatttgaagtcagaggttta Mask Tandem Repeat Region ================================================ taaacctctgacttcaaatttgtaacaaatgctacatggatcacatttcaatgaaaaggaggttgattttcccttgcactctgaatttattagaggataattattatcaattacatcaagcaggaatagttttacgataaggagatgggat Find is-nt database================================================ Query_seq: AA02433:AA02434|AA02433:AA02434:hypothetical protein:membrane protein:->->:1678536..1678686 151 taaacctctgacttcaaatttgtaacaaatgctacatggatcacatttcaatgaaaaggaggttgattttcccttgcactctgaatttattagaggataattattatcaattacatcaagcaggaatagttttacgataaggagatgggat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02433:AA02434|AA02433:AA02434:hypothetical protein:membrane protein:->->:1678536..1678686 151 taaacctctgacttcaaatttgtaacaaatgctacatggatcacatttcaatgaaaaggaggttgattttcccttgcactctgaatttattagaggataattattatcaattacatcaagcaggaatagttttacgataaggagatgggat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02433:AA02434|AA02433:AA02434:hypothetical protein:membrane protein:->->:1678536..1678686 151 taaacctctgacttcaaatttgtaacaaatgctacatggatcacatttcaatgaaaaggaggttgattttcccttgcactctgaatttattagaggataattattatcaattacatcaagcaggaatagttttacgataaggagatgggat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaacctctgacttcaaatttgtaacaaatgctacatggatcacatttcaatgaaaaggaggttgattttcccttgcactctgaatttattagaggataattattatcaattacatcaagcaggaatagttttacgataaggagatgggat Predict ORF larger than 30AA ================================================ Protein_Len: 40 Strand: + Start: 18 End: 137 ................. M C N K C Y M D H I S M K R R L I F P C T L N L L E D N Y Y Q L H Q A G I V L R .............. Protein_Len: 46 Strand: - Start: 1 End: 138 L G R V E F K Y C I S C P D C K L S F S T S K G K C E S N I L P Y N N D I V D L L F L K V M ............. taaacctctgacttcaaatttgtaacaaatgctacatggatcacatttcaatgaaaaggaggttgattttcccttgcactctgaatttattagaggataattattatcaattacatcaagcaggaatagttttacgataaggagatgggat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaacctctgacttcaaatttgtaacaaatgctacatggatcacatttcaatgaaaaggaggttgattttcccttgcactctgaatttattagaggataattattatcaattacatcaagcaggaatagttttacgataaggagatgggat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02433:AA02434|AA02433:AA02434:hypothetical protein:membrane protein:->->:1678536..1678686 151 taaacctctgacttcaaatttgtaacaaatgctacatggatcacatttcaatgaaaaggaggttgattttcccttgcactctgaatttattagaggataattattatcaattacatcaagcaggaatagttttacgataaggagatgggat Intra-Species Hit: Count: 1 Min: 1 Max: 151 Len: 151 Subject: aact_AA02433_AA02434|hypothetical protein:membrane protein|POSITIVE:POSITIVE|[1678536,1678686]|151 HSP 1 e-value: 1.0E-80 bit: 299.0 Len: 151 Query Start:1 Query End:151 Subject Strand: POSITIVE Subject Start: 1 Subject End: 151 taaacctctgacttcaaatttgtaacaaatgctacatggatcacatttcaatgaaaaggaggttgattttcccttgcactctgaatttattagaggataattattatcaattacatcaagcaggaatagttttacgataaggagatgggat taaacctctgacttcaaatttgtaacaaatgctacatggatcacatttcaatgaaaaggaggttgattttcccttgcactctgaatttattagaggataattattatcaattacatcaagcaggaatagttttacgataaggagatgggat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACCUCUGACUUCAAAUUUGUAACAAAUGCUACAUGGAUCACAUUUCAAUGAAAAGGAGGUUGAUUUUCCCUUGCACUCUGAAUUUAUUAGAGGAUAAUUAUUAUCAAUUACAUCAAGCAGGAAUAGUUUUACGAUAAGGAGAUGGGAU ..(((((((...((((....((((.(....).)))).(((......)))..))))..))))))).....((((.(((.((((((.....))))))((((.....))))...........)))......(((((........))))))))). (-26.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUCCCAUCUCCUUAUCGUAAAACUAUUCCUGCUUGAUGUAAUUGAUAAUAAUUAUCCUCUAAUAAAUUCAGAGUGCAAGGGAAAAUCAACCUCCUUUUCAUUGAAAUGUGAUCCAUGUAGCAUUUGUUACAAAUUUGAAGUCAGAGGUUUA .((((......((((((....((...((......)).))...))))))........((((.........)))).....)))).....((((((....((((......))))....((((((....))))))............)))))).. (-24.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 260 84 44 187 149 aact:AA00782|5end_conserved_hypothetical_protein_538723..538953_POSITIVE 241 73 45 172 144 aact:AA00311|5end_L-fuculose-phosphate_aldolase_211434..211664_POSITIVE 239 77 31 143 78 aact:AA01532|5end_hypothetical_protein_1028771..1029001_POSITIVE 239 77 31 77 12 aact:AA01531|5end_hypothetical_protein_1028705..1028935_POSITIVE 231 149 103 188 140 aact:AA00033|5end_conserved_hypothetical_protein_25643..25873_POSITIVE 230 70 30 164 124 aact:AA00106|5end_1-hydroxy-2-methyl-2-(E)-butenyl_4-diphosphate_synthase_75544..75774_POSITIVE 228 95 56 206 166 aact:AA02284|5end_phosphoglycerate_transport;_protein_for_signal_transmission_1553978..1554208_POSITIVE 227 80 31 102 61 aact:AA02992|5end_RNA_methyltransferase_2090638..2090868_POSITIVE 227 98 58 72 26 aact:AA00902|5end_RNA_polymerase_sigma-E_factor_sigma-24_623829..624059_POSITIVE 225 80 35 132 92 aact:AA01146|5end_conserved_hypothetical_protein_784433..784663_POSITIVE 224 126 81 120 73 aact:AA02686|5end_hydrogenase_4_component_C_1871546..1871776_POSITIVE 222 149 101 127 82 aact:AA00084|5end_aminotransferase_60814..61044_POSITIVE 221 85 44 49 5 aact:AA03016|5end_hypothetical_protein_2105144..2105374_POSITIVE 221 70 26 180 138 aact:AA02316|5end_exodeoxyribonuclease_VII_large_subunit_1581682..1581912_POSITIVE 219 83 45 158 126 aact:AA02731|5end_outer_membrane_lipoprotein_carrier_precursor_1911498..1911728_POSITIVE 217 72 31 77 46 aact:AA01115|5end_carboxylesterase_766545..766775_POSITIVE 216 83 42 155 108 aact:AA00822|5end_conserved_hypothetical_protein_(possible_arsenate_reductase)_564596..564826_POSITIVE 216 150 112 228 193 aact:AA00122|5end_hypothetical_protein_90212..90442_POSITIVE 215 150 103 206 159 aact:AA02974|5end_conserved_hypothetical_protein_2078990..2079220_POSITIVE 215 134 102 43 5 aact:AA00277|5end_biotin_synthesis_protein_193873..194103_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 233 86 49 79 36 aact:AA00249|3end_cysteinyl-tRNA_synthetase_(cysteine--tRNA_ligase)_175422..175572_POSITIVE 230 70 30 149 109 aact:AA00105|3end_conserved_hypothetical_protein_75609..75759_POSITIVE 225 80 35 79 39 aact:AA01145|3end_glycyl-tRNA_synthetase_alpha_subunit_784460..784610_POSITIVE 224 126 81 106 59 aact:AA02684|3end_hydrogenase_4_membrane_subunit_1871612..1871762_POSITIVE 222 149 101 84 39 aact:AA00083|3end_hypothetical_protein_60851..61001_POSITIVE 221 85 44 76 32 aact:AA03015|3end_hypothetical_protein_2105251..2105401_POSITIVE 219 83 45 142 110 aact:AA02728|3end_conserved_hypothetical_protein_(possible_ATPase)_1911562..1911712_POSITIVE 218 57 12 133 82 aact:AA01531|3end_hypothetical_protein_1028877..1029027_POSITIVE 216 148 101 139 86 aact:AA01670|3end_conserved_hypothetical_protein_1130761..1130911_POSITIVE 215 150 103 134 87 aact:AA02973|3end_tyrosine-specific_transport_protein_2078998..2079148_POSITIVE 215 134 102 40 2 aact:AA00276|3end_dethiobiotin_synthase_193950..194100_POSITIVE 214 79 35 45 3 aact:AA02869|3end_hypothetical_protein_2013972..2014122_POSITIVE 213 83 59 30 5 aact:AA00635|3end_dihydropteroate_synthase_436828..436978_POSITIVE 212 80 50 113 82 aact:AA02074|3end_hypothetical_protein_1409656..1409806_POSITIVE 211 93 51 84 44 aact:AA01199|3end_conserved_hypothetical_protein_822486..822636_POSITIVE 207 68 28 149 111 aact:AA00612|3end_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosami_ne_418705..418855_POSITIVE 201 80 39 70 36 aact:AA02830|3end_colicin_tolerance_protein_1990086..1990236_POSITIVE 201 83 48 124 88 aact:AA00326|3end_periplasmic_oxidoreductase_(por)_(Thiol:disulfide_interchange_protein_dsbA_precursor.)_222397..222547_POSITIVE 197 136 93 125 77 aact:AA01039|3end_transcriptional_regulator_(possible_murein_gene_regulator)_724252..724402_POSITIVE 196 68 25 77 16 aact:AA01274|3end_hypothetical_protein_864832..864982_POSITIVE