Origin IGS: tatattcatctccctgttaaaaattcgtacgtagaattatagcacttacctattgaaaaatccttgataaattcttgaatggaatagtgggttaatggataatacagcctcgactaaatcgtggggaattaca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgtaattccccacgatttagtcgaggctgtattatccattaacccactattccattcaagaatttatcaaggatttttcaataggtaagtgctataattctacgtacgaatttttaacagggagatgaatata Mask Tandem Repeat Region ================================================ tatattcatctccctgttaaaaattcgtacgtagaattatagcacttacctattgaaaaatccttgataaattcttgaatggaatagtgggttaatggataatacagcctcgactaaatcgtggggaattaca Find is-nt database================================================ Query_seq: AA02429:AA02430|AA02429:AA02430:methionine aminopeptidase:conserved hypothetical protein:->->:1675258..1675390 133 tatattcatctccctgttaaaaattcgtacgtagaattatagcacttacctattgaaaaatccttgataaattcttgaatggaatagtgggttaatggataatacagcctcgactaaatcgtggggaattaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02429:AA02430|AA02429:AA02430:methionine aminopeptidase:conserved hypothetical protein:->->:1675258..1675390 133 tatattcatctccctgttaaaaattcgtacgtagaattatagcacttacctattgaaaaatccttgataaattcttgaatggaatagtgggttaatggataatacagcctcgactaaatcgtggggaattaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02429:AA02430|AA02429:AA02430:methionine aminopeptidase:conserved hypothetical protein:->->:1675258..1675390 133 tatattcatctccctgttaaaaattcgtacgtagaattatagcacttacctattgaaaaatccttgataaattcttgaatggaatagtgggttaatggataatacagcctcgactaaatcgtggggaattaca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tatattcatctccctgttaaaaattcgtacgtagaattatagcacttacctattgaaaaatccttgataaattcttgaatggaatagtgggttaatggataatacagcctcgactaaatcgtggggaattaca Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: - Start: 9 End: 107 ........ R G T L F E Y T S N Y C K G I S F D K I F E Q I S Y H T L P Y Y M .......................... tatattcatctccctgttaaaaattcgtacgtagaattatagcacttacctattgaaaaatccttgataaattcttgaatggaatagtgggttaatggataatacagcctcgactaaatcgtggggaattaca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tatattcatctccctgttaaaaattcgtacgtagaattatagcacttacctattgaaaaatccttgataaattcttgaatggaatagtgggttaatggataatacagcctcgactaaatcgtggggaattaca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02429:AA02430|AA02429:AA02430:methionine aminopeptidase:conserved hypothetical protein:->->:1675258..1675390 133 tatattcatctccctgttaaaaattcgtacgtagaattatagcacttacctattgaaaaatccttgataaattcttgaatggaatagtgggttaatggataatacagcctcgactaaatcgtggggaattaca Intra-Species Hit: Count: 1 Min: 1 Max: 133 Len: 133 Subject: aact_AA02429_AA02430|methionine aminopeptidase:conserved hypothetical protein|POSITIVE:POSITIVE|[1675258,1675390]|133 HSP 1 e-value: 7.0E-70 bit: 264.0 Len: 133 Query Start:1 Query End:133 Subject Strand: POSITIVE Subject Start: 1 Subject End: 133 tatattcatctccctgttaaaaattcgtacgtagaattatagcacttacctattgaaaaatccttgataaattcttgaatggaatagtgggttaatggataatacagcctcgactaaatcgtggggaattaca tatattcatctccctgttaaaaattcgtacgtagaattatagcacttacctattgaaaaatccttgataaattcttgaatggaatagtgggttaatggataatacagcctcgactaaatcgtggggaattaca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUAUUCAUCUCCCUGUUAAAAAUUCGUACGUAGAAUUAUAGCACUUACCUAUUGAAAAAUCCUUGAUAAAUUCUUGAAUGGAAUAGUGGGUUAAUGGAUAAUACAGCCUCGACUAAAUCGUGGGGAAUUACA ..........((((..(.......(((...((.(.(((((..((...((((((((....((.(..((.....))..).))....))))))))...)).))))).).))..))).......)..))))...... (-21.24) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUAAUUCCCCACGAUUUAGUCGAGGCUGUAUUAUCCAUUAACCCACUAUUCCAUUCAAGAAUUUAUCAAGGAUUUUUCAAUAGGUAAGUGCUAUAAUUCUACGUACGAAUUUUUAACAGGGAGAUGAAUAUA .....(((((..((((...))))....(((((.....((((...((((...(((((.((((((((.....)))))))).))).))..))))...)))).....)))))...........)))))......... (-19.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 295 125 86 54 15 aact:AA02972|5end_conserved_hypothetical_protein_(tyrosine_phenol_lyase-related_protein)_2077381..2077611_POSITIVE 293 119 73 57 6 aact:AA02742|5end_tRNA_nucleotidyltransferase_1921308..1921538_POSITIVE 290 93 55 153 120 aact:AA02237|5end_molybdopterin_biosynthesis_protein_E_chain_1522760..1522990_POSITIVE 276 127 81 120 67 aact:AA00679|5end_cytoplasmic_axial_filament_(Ribonuclease_G)_463288..463518_POSITIVE 261 128 91 153 113 aact:AA01950|5end_ABC_transporter,_membrane_spanning_protein_1318116..1318346_POSITIVE 257 123 81 100 53 aact:AA01288|5end_hypothetical_protein_872086..872316_POSITIVE 257 120 72 212 170 aact:AA00292|5end_dihydrofolate_reductase_201800..202030_POSITIVE 257 120 72 127 85 aact:AA00291|5end_hypothetical_protein_201715..201945_POSITIVE 255 128 81 229 166 aact:AA00803|5end_hypothetical_protein_549526..549756_POSITIVE 253 109 61 141 98 aact:AA00909|5end_molybdopterin-guanine_dinucleotide_biosynthesis_protein_B_626594..626824_POSITIVE 245 128 86 131 90 aact:AA00013|5end_hypothetical_protein_6695..6925_POSITIVE 240 115 80 108 75 aact:AA02409|5end_DNA_ligase_1653315..1653545_POSITIVE 234 129 81 118 71 aact:AA01449|5end_prophage_CP4-57_integrase_970569..970799_POSITIVE 234 126 82 132 74 aact:AA00046|5end_formate_dependent_nitrite_reductase,_cytochrome-c_type_protein_36014..36244_POSITIVE 232 128 81 55 10 aact:AA00260|5end_conserved_hypothetical_protein_183315..183545_POSITIVE 231 59 16 115 70 aact:AA03011|5end_inner_membrane_protein,_60_kDa_2102585..2102815_POSITIVE 229 109 63 97 38 aact:AA01211|5end_ATP-dependent_Clp_protease,_ATPase_subunit_827035..827265_POSITIVE 228 128 97 224 188 aact:AA01825|5end_iron(III)_ABC_transporter,_permease_protein_1228475..1228705_POSITIVE 226 128 87 60 29 aact:AA01956|5end_3-deoxy-manno-octulosonate_cytidylyltransferase_1322436..1322666_POSITIVE 226 130 81 214 150 aact:AA01151|5end_porphobilinogen_synthase_(delta-aminolevulinic_acid_dehydratase)_788323..788553_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 293 119 73 63 12 aact:AA02740|3end_conserved_hypothetical_protein_1921394..1921544_POSITIVE 257 123 81 103 56 aact:AA01287|3end_transposase_872169..872319_POSITIVE 257 120 72 145 103 aact:AA00290|3end_conserved_hypothetical_protein_201813..201963_POSITIVE 253 109 61 57 14 aact:AA00908|3end_probable_sigma-E_factor_regulatory_protein_626590..626740_POSITIVE 250 127 84 46 7 aact:AA02191|3end_hypothetical_protein_1493772..1493922_POSITIVE 245 128 86 135 94 aact:AA00012|3end_conserved_hypothetical_protein_6779..6929_POSITIVE 234 48 8 54 21 aact:AA02568|3end_sugar_phosphotransferase_component_II_B_1788725..1788875_POSITIVE 234 126 82 132 74 aact:AA00044|3end_formate_dependent_nitrite_reductase,_Fe-S_protein/_anaerobic_DMSO_reductase_B_36094..36244_POSITIVE 229 109 63 81 22 aact:AA01210|3end_hypothetical_protein_827099..827249_POSITIVE 226 128 90 76 38 aact:AA01393|3end_ABC_transporter,_ATP-binding_protein_942029..942179_POSITIVE 223 125 88 62 29 aact:AA01543|3end_hypothetical_protein_1034350..1034500_POSITIVE 221 130 81 44 1 aact:AA02109|3end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1436933..1437083_POSITIVE 221 125 104 51 32 aact:AA00332|3end_PTS_system,_fructose-specific_IIA/FPr_component_(diphosphoryl_transfer_protein)_225617..225767_POSITIVE 220 128 81 127 81 aact:AA02201|3end_transferrin-binding_protein_1_precursor_1499265..1499415_POSITIVE 220 123 91 148 113 aact:AA01948|3end_ABC_transporter_permease,_membrane_spanning_protein_1318196..1318346_POSITIVE 218 126 85 138 89 aact:AA00177|3end_conserved_hypothetical_protein_126313..126463_POSITIVE 216 126 81 86 30 aact:AA02237|3end_molybdopterin_biosynthesis_protein_E_chain_1523292..1523442_POSITIVE 214 127 85 77 34 aact:AA00820|3end_succinyl-diaminopimelate_desuccinylase_564536..564686_POSITIVE 211 128 81 147 97 aact:AA01873|3end_mannose-6-phosphate_isomerase_1261940..1262090_POSITIVE 211 129 84 92 38 aact:AA00521|3end_phosphatase_358324..358474_POSITIVE