Origin IGS: aggtcttcccgtaaaaataaagtgcgcaaagcatatcacaaaggcaaaaggctacataaatatttttactaacctcctattttttcagtacaatgtcacacattttttgaacgtaaggataaaaaacg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cgttttttatccttacgttcaaaaaatgtgtgacattgtactgaaaaaataggaggttagtaaaaatatttatgtagccttttgcctttgtgatatgctttgcgcactttatttttacgggaagacct Mask Tandem Repeat Region ================================================ aggtcttcccgtaaaaataaagtgcgcaaagcatatcacaaaggcaaaaggctacataaatatttttactaacctcctattttttcagtacaatgtcacacattttttgaacgtaaggataaaaaacg Find is-nt database================================================ Query_seq: AA02423:AA02425|AA02423:AA02425:undecaprenyl-phosphate alpha-N-acetylglucosaminyltransferase:glutamate-1-semialdehyde aminotransferase:->->:1670313..1670440 128 aggtcttcccgtaaaaataaagtgcgcaaagcatatcacaaaggcaaaaggctacataaatatttttactaacctcctattttttcagtacaatgtcacacattttttgaacgtaaggataaaaaacg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02423:AA02425|AA02423:AA02425:undecaprenyl-phosphate alpha-N-acetylglucosaminyltransferase:glutamate-1-semialdehyde aminotransferase:->->:1670313..1670440 128 aggtcttcccgtaaaaataaagtgcgcaaagcatatcacaaaggcaaaaggctacataaatatttttactaacctcctattttttcagtacaatgtcacacattttttgaacgtaaggataaaaaacg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02423:AA02425|AA02423:AA02425:undecaprenyl-phosphate alpha-N-acetylglucosaminyltransferase:glutamate-1-semialdehyde aminotransferase:->->:1670313..1670440 128 aggtcttcccgtaaaaataaagtgcgcaaagcatatcacaaaggcaaaaggctacataaatatttttactaacctcctattttttcagtacaatgtcacacattttttgaacgtaaggataaaaaacg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aggtcttcccgtaaaaataaagtgcgcaaagcatatcacaaaggcaaaaggctacataaatatttttactaacctcctattttttcagtacaatgtcacacattttttgaacgtaaggataaaaaacg Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 17 End: 127 ................ M K C A K H I T K A K G Y I N I F T N L L F F Q Y N V T H F L N V R I K N . aggtcttcccgtaaaaataaagtgcgcaaagcatatcacaaaggcaaaaggctacataaatatttttactaacctcctattttttcagtacaatgtcacacattttttgaacgtaaggataaaaaacg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aggtcttcccgtaaaaataaagtgcgcaaagcatatcacaaaggcaaaaggctacataaatatttttactaacctcctattttttcagtacaatgtcacacattttttgaacgtaaggataaaaaacg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02423:AA02425|AA02423:AA02425:undecaprenyl-phosphate alpha-N-acetylglucosaminyltransferase:glutamate-1-semialdehyde aminotransferase:->->:1670313..1670440 128 aggtcttcccgtaaaaataaagtgcgcaaagcatatcacaaaggcaaaaggctacataaatatttttactaacctcctattttttcagtacaatgtcacacattttttgaacgtaaggataaaaaacg Intra-Species Hit: Count: 1 Min: 1 Max: 128 Len: 128 Subject: aact_AA02423_AA02425|undecaprenyl-phosphate alpha-N-acetylglucosaminyltransferase:glutamate-1- semialdehyde aminotransferase|POSITIVE:POSITIVE|[1670313,1670440]|128 HSP 1 e-value: 6.0E-67 bit: 254.0 Len: 128 Query Start:1 Query End:128 Subject Strand: POSITIVE Subject Start: 1 Subject End: 128 aggtcttcccgtaaaaataaagtgcgcaaagcatatcacaaaggcaaaaggctacataaatatttttactaacctcctattttttcagtacaatgtcacacattttttgaacgtaaggataaaaaacg aggtcttcccgtaaaaataaagtgcgcaaagcatatcacaaaggcaaaaggctacataaatatttttactaacctcctattttttcagtacaatgtcacacattttttgaacgtaaggataaaaaacg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGGUCUUCCCGUAAAAAUAAAGUGCGCAAAGCAUAUCACAAAGGCAAAAGGCUACAUAAAUAUUUUUACUAACCUCCUAUUUUUUCAGUACAAUGUCACACAUUUUUUGAACGUAAGGAUAAAAAACG ..........(((((((((..((((.....))))........(((.....))).......))))))))).....((((.....(((((...((((.....))))..)))))....))))......... (-19.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGUUUUUUAUCCUUACGUUCAAAAAAUGUGUGACAUUGUACUGAAAAAAUAGGAGGUUAGUAAAAAUAUUUAUGUAGCCUUUUGCCUUUGUGAUAUGCUUUGCGCACUUUAUUUUUACGGGAAGACCU ...........(((.(((..(((.((.(((((.((..(((....(((..(((((((((((((((....))))).))))))))))..)))......)))..))))))).)).)))..)))..))).... (-23.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 251 46 2 83 41 aact:AA01603|5end_oligopeptidase_A_1082898..1083128_POSITIVE 248 46 1 79 28 aact:AA00506|5end_ATP-dependent_RNA_helicase_protein_351681..351911_POSITIVE 247 53 16 127 95 aact:AA00745|5end_galactokinase_511163..511393_POSITIVE 243 70 22 231 175 aact:AA00358|5end_30S_ribosomal_protein_S17_243975..244205_POSITIVE 242 53 17 103 63 aact:AA00141|5end_hypoxanthine_phosphoribosyl_transferase_(HPRT)_100814..101044_POSITIVE 232 52 23 228 200 aact:AA02961|5end_pyrroline-5-carboxylate_reductase_2070785..2071015_POSITIVE 231 52 21 137 93 aact:AA01608|5end_conserved_hypothetical_protein_1087313..1087543_POSITIVE 231 44 1 77 37 aact:AA00125|5end_conserved_hypothetical_protein_(possible_phage-related_protein)_90586..90816_POSITIVE 229 90 41 54 10 aact:AA02092|5end_glycogen_synthase_1424674..1424904_POSITIVE 225 45 3 175 131 aact:AA02936|5end_lipoprotein_E_precursor;_outer_membrane_protein_P4_precursor;_acid_phosphatase_2055610..2055840_POSITIVE 224 45 1 167 122 aact:AA01867|5end_thiamine_transport_ATP-binding_protein_1254853..1255083_POSITIVE 224 44 1 141 93 aact:AA00950|5end_conserved_hypothetical_protein_656922..657152_POSITIVE 223 60 20 44 15 aact:AA02933|5end_conserved_hypothetical_protein_(HesA/MoeB/ThiF_family_protein)_2053467..2053697_POSITIVE 222 65 22 213 156 aact:AA02149|5end_possible_transposase_1469918..1470148_POSITIVE 222 61 26 59 19 aact:AA01875|5end_PTS_system,_glucose-specific_enzyme_II,_A_component_1262563..1262793_POSITIVE 219 44 10 105 63 aact:AA01152|5end_sec-independent_protein_translocase_protein_789364..789594_POSITIVE 217 36 2 60 24 aact:AA02213|5end_hypothetical_protein_1506996..1507226_POSITIVE 215 44 1 73 34 aact:AA02615|5end_conserved_hypothetical_protein_1823040..1823270_POSITIVE 214 55 14 63 23 aact:AA00601|5end_hypothetical_protein_408863..409093_POSITIVE 213 49 8 64 22 aact:AA01524|5end_amino_acid_ABC_transporter,_ATP-binding_protein_1023500..1023730_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 247 53 16 130 98 aact:AA00744|3end_aldose_1-epimerase_(mutarotase)_511246..511396_POSITIVE 242 53 17 81 41 aact:AA00140|3end_hypothetical_protein_100872..101022_POSITIVE 239 46 10 51 4 aact:AA00505|3end_hypothetical_protein_351733..351883_POSITIVE 231 44 1 81 41 aact:AA00124|3end_conserved_hypothetical_protein_(possible_cytoplasmic_protein)_90670..90820_POSITIVE 223 60 20 41 12 aact:AA02932|3end_lytic_murein_transglycosylase_A_2053544..2053694_POSITIVE 219 44 10 83 41 aact:AA01151|3end_porphobilinogen_synthase_(delta-aminolevulinic_acid_dehydratase)_789422..789572_POSITIVE 217 36 2 149 113 aact:AA02213|3end_hypothetical_protein_1507165..1507315_POSITIVE 217 36 2 62 26 aact:AA02212|3end_ribonucleoside-diphosphate_reductase_beta_chain_1507078..1507228_POSITIVE 217 48 8 47 6 aact:AA01984|3end_tellurite_resistance_protein;_hemagglutinin_homolog_1345754..1345904_POSITIVE 214 45 2 113 69 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 214 55 14 59 19 aact:AA00600|3end_ribosome_recycling_factor_(ribosome_releasing_factor)_408939..409089_POSITIVE 213 47 1 86 23 aact:AA01893|3end_acetyl-coenzyme-A_carboxylase_subunit_A_1277872..1278022_POSITIVE 213 49 8 53 11 aact:AA01523|3end_conserved_hypothetical_protein_1023569..1023719_POSITIVE 210 54 23 63 37 aact:AA01887|3end_conserved_hypothetical_protein_(possible_cytochrome_1273134..1273284_POSITIVE 210 49 9 82 52 aact:AA00495|3end_hypothetical_protein_345139..345289_POSITIVE 207 45 2 64 18 aact:AA00686|3end_hypothetical_protein_469219..469369_POSITIVE 206 46 5 38 1 aact:AA01668|3end_transcription_elongation_factor_1130383..1130533_POSITIVE 206 57 21 88 57 aact:AA00162|3end_pilin_C_biogenesis_protein/protein_transport_protein_116086..116236_POSITIVE 206 52 20 149 122 aact:AA00112|3end_uracil_permease_81239..81389_POSITIVE 204 46 9 47 2 aact:AA02261|3end_30S_ribosomal_protein_S9_1538482..1538632_POSITIVE