Origin IGS: aatttttccttatgtttggggactgtggacgtttcattttgcatttgagctagcatttatttttatgataacaaggcaaaataaacctagccctttgggttgtgcctttaggcgacaattcaaatgcaaaatgaaacgtccacagccccgagtaaaacgatcgtaaaaacgaccgcactttttgttcatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatgaacaaaaagtgcggtcgtttttacgatcgttttactcggggctgtggacgtttcattttgcatttgaattgtcgcctaaaggcacaacccaaagggctaggtttattttgccttgttatcataaaaataaatgctagctcaaatgcaaaatgaaacgtccacagtccccaaacataaggaaaaatt Mask Tandem Repeat Region ================================================ aatttttccttatgtttggggactgtggacgtttcattttgcatttgagctagcatttatttttatgataacaaggcaaaataaacctagccctttgggttgtgcctttaggcgacaattcaaatgcaaaatgaaacgtccacagccccgagtaaaacgatcgtaaaaacgaccgcactttttgttcatta Find is-nt database================================================ Query_seq: AA02417:AA02418|AA02417:AA02418:GTP-binding protein:peptidyl-tRNA hydrolase:->->:1661039..1661229 191 aatttttccttatgtttggggactgtggacgtttcattttgcatttgagctagcatttatttttatgataacaaggcaaaataaacctagccctttgggttgtgcctttaggcgacaattcaaatgcaaaatgaaacgtccacagccccgagtaaaacgatcgtaaaaacgaccgcactttttgttcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02417:AA02418|AA02417:AA02418:GTP-binding protein:peptidyl-tRNA hydrolase:->->:1661039..1661229 191 aatttttccttatgtttggggactgtggacgtttcattttgcatttgagctagcatttatttttatgataacaaggcaaaataaacctagccctttgggttgtgcctttaggcgacaattcaaatgcaaaatgaaacgtccacagccccgagtaaaacgatcgtaaaaacgaccgcactttttgttcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02417:AA02418|AA02417:AA02418:GTP-binding protein:peptidyl-tRNA hydrolase:->->:1661039..1661229 191 aatttttccttatgtttggggactgtggacgtttcattttgcatttgagctagcatttatttttatgataacaaggcaaaataaacctagccctttgggttgtgcctttaggcgacaattcaaatgcaaaatgaaacgtccacagccccgagtaaaacgatcgtaaaaacgaccgcactttttgttcatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatttttccttatgtttggggactgtggacgtttcattttgcatttgagctagcatttatttttatgataacaaggcaaaataaacctagccctttgggttgtgcctttaggcgacaattcaaatgcaaaatgaaacgtccacagccccgagtaaaacgatcgtaaaaacgaccgcactttttgttcatta Predict ORF larger than 30AA ================================================ Protein_Len: 54 Strand: + Start: 2 End: 163 . M F P Y V W G L W T F H F A F E L A F I F M I T R Q N K P S P L G C A F R R Q F K C K M K R P Q P R V K R S ............................ Protein_Len: 42 Strand: - Start: 53 End: 178 .................................................... C K N K H Y C P L I F R A R Q T T G K P S L E F A F H F T W L G S Y F S R L F S R M ............. Protein_Len: 51 Strand: - Start: 37 End: 189 .................................... K A N S S A N I K I I V L C F L G L G K P Q A K L R C N L H L I F R G C G R T F R D Y F R G C K K N M .. aatttttccttatgtttggggactgtggacgtttcattttgcatttgagctagcatttatttttatgataacaaggcaaaataaacctagccctttgggttgtgcctttaggcgacaattcaaatgcaaaatgaaacgtccacagccccgagtaaaacgatcgtaaaaacgaccgcactttttgttcatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatttttccttatgtttggggactgtggacgtttcattttgcatttgagctagcatttatttttatgataacaaggcaaaataaacctagccctttgggttgtgcctttaggcgacaattcaaatgcaaaatgaaacgtccacagccccgagtaaaacgatcgtaaaaacgaccgcactttttgttcatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02417:AA02418|AA02417:AA02418:GTP-binding protein:peptidyl-tRNA hydrolase:->->:1661039..1661229 191 aatttttccttatgtttggggactgtggacgtttcattttgcatttgagctagcatttatttttatgataacaaggcaaaataaacctagccctttgggttgtgcctttaggcgacaattcaaatgcaaaatgaaacgtccacagccccgagtaaaacgatcgtaaaaacgaccgcactttttgttcatta Intra-Species Hit: Count: 1 Min: 1 Max: 191 Len: 191 Subject: aact_AA02417_AA02418|GTP-binding protein:peptidyl-tRNA hydrolase|POSITIVE:POSITIVE|[1661039,1661229]|191 HSP 1 e-value: 1.0E-104 bit: 379.0 Len: 191 Query Start:1 Query End:191 Subject Strand: POSITIVE Subject Start: 1 Subject End: 191 aatttttccttatgtttggggactgtggacgtttcattttgcatttgagctagcatttatttttatgataacaaggcaaaataaacctagccctttgggttgtgcctttaggcgacaattcaaatgcaaaatgaaacgtccacagccccgagtaaaacgatcgtaaaaacgaccgcactttttgttcatta aatttttccttatgtttggggactgtggacgtttcattttgcatttgagctagcatttatttttatgataacaaggcaaaataaacctagccctttgggttgtgcctttaggcgacaattcaaatgcaaaatgaaacgtccacagccccgagtaaaacgatcgtaaaaacgaccgcactttttgttcatta HSP 2 e-value: 7.0E-6 bit: 52.0 Len: 26 Query Start:120 Query End:145 Subject Strand: NEGATIVE Subject Start: 23 Subject End: 48 .......................................................................................................................tcaaatgcaaaatgaaacgtccacag.............................................. .......................................................................................................................tcaaatgcaaaatgaaacgtccacag.............................................. HSP 3 e-value: 7.0E-6 bit: 52.0 Len: 26 Query Start:23 Query End:48 Subject Strand: NEGATIVE Subject Start: 120 Subject End: 145 ......................ctgtggacgtttcattttgcatttga............................................................................................................................................... ......................ctgtggacgtttcattttgcatttga............................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUUUUCCUUAUGUUUGGGGACUGUGGACGUUUCAUUUUGCAUUUGAGCUAGCAUUUAUUUUUAUGAUAACAAGGCAAAAUAAACCUAGCCCUUUGGGUUGUGCCUUUAGGCGACAAUUCAAAUGCAAAAUGAAACGUCCACAGCCCCGAGUAAAACGAUCGUAAAAACGACCGCACUUUUUGUUCAUUA ..............(((((((.(((((((((((((((((((((((((((...((..(((((.....))))).((((((.....(((((((....))))))).))))))...)).....)))))))))))))))))))))))))))))))))).....((.((((....)))).))................ (-74.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGAACAAAAAGUGCGGUCGUUUUUACGAUCGUUUUACUCGGGGCUGUGGACGUUUCAUUUUGCAUUUGAAUUGUCGCCUAAAGGCACAACCCAAAGGGCUAGGUUUAUUUUGCCUUGUUAUCAUAAAAAUAAAUGCUAGCUCAAAUGCAAAAUGAAACGUCCACAGUCCCCAAACAUAAGGAAAAAUU ..(((.......(((((((((((....))))))))...))).(((((((((((((((((((((((((((((..((((.(((....))))))).......(((((((((((((((....((....))..))))))))).)))))))))))))))))))))))))))))))))))....)))........... (-81.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 443 185 141 180 133 aact:AA00212|5end_ribokinase_146056..146286_POSITIVE 443 185 141 79 32 aact:AA00211|5end_hypothetical_protein_145955..146185_POSITIVE 401 181 142 212 167 aact:AA00347|5end_50S_ribosomal_protein_L11_232137..232367_POSITIVE 396 188 144 144 99 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 388 180 137 121 71 aact:AA01603|5end_oligopeptidase_A_1082898..1083128_POSITIVE 383 184 147 125 85 aact:AA02937|5end_conserved_hypothetical_protein_2056379..2056609_POSITIVE 382 186 147 181 142 aact:AA00819|5end_D-alanyl-D-alanine_carboxypeptidase_562644..562874_POSITIVE 379 180 131 60 10 aact:AA02091|5end_hypothetical_protein_1424441..1424671_POSITIVE 361 188 143 51 3 aact:AA02507|5end_penicillin-insensitive_murein_endopeptidase_A_1740058..1740288_POSITIVE 356 180 131 149 96 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 356 185 142 197 151 aact:AA00268|5end_acyl_carrier_protein_(ACP)_188104..188334_POSITIVE 352 180 137 79 31 aact:AA01969|5end_hypothetical_protein_1331969..1332199_POSITIVE 352 190 145 131 85 aact:AA00792|5end_conserved_hypothetical_protein_543142..543372_POSITIVE 344 185 147 46 5 aact:AA00267|5end_hypothetical_protein_187953..188183_POSITIVE 341 180 136 220 153 aact:AA01424|5end_threonine_deaminase_(dehydratase)_957740..957970_POSITIVE 338 187 149 145 101 aact:AA00409|5end_methionyl-tRNA_formyltransferase_273611..273841_POSITIVE 335 180 131 62 10 aact:AA00349|5end_hypothetical_protein_233274..233504_POSITIVE 332 185 154 128 97 aact:AA02716|5end_peptidase_T_1899789..1900019_POSITIVE 332 189 142 61 18 aact:AA01204|5end_hypothetical_protein_825015..825245_POSITIVE 330 190 148 58 12 aact:AA02846|5end_crossover_junction_endodeoxyribonuclease_1998648..1998878_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 505 154 121 78 44 aact:AA02417|3end_GTP-binding_protein_1660978..1661128_POSITIVE 443 185 141 76 29 aact:AA00209|3end_D-ribose_ABC_transporter,_periplasmic-binding_protein_146032..146182_POSITIVE 408 48 6 60 13 aact:AA01115|3end_carboxylesterase_767353..767503_POSITIVE 401 181 142 53 8 aact:AA00345|3end_transcription_antitermination_protein_232058..232208_POSITIVE 389 188 153 39 4 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 379 180 131 88 38 aact:AA02090|3end_glucose-1-phosphate_adenylyltransferase_1424549..1424699_POSITIVE 363 184 149 39 2 aact:AA02936|3end_lipoprotein_E_precursor;_outer_membrane_protein_P4_precursor;_acid_phosphatase_2056373..2056523_POSITIVE 356 180 131 144 91 aact:AA00323|3end_conserved_hypothetical_protein_(possible_SAM-dependent_methyltransferase)_220222..220372_POSITIVE 341 180 136 80 13 aact:AA01423|3end_cell_division_protein_X,_ABC_system_permease_957680..957830_POSITIVE 338 187 149 142 98 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 336 190 154 43 7 aact:AA00790|3end_recombination_DNA_repair_protein_543134..543284_POSITIVE 332 185 154 46 15 aact:AA02715|3end_paraquat-inducible_protein_pqiB_homolog_1899787..1899937_POSITIVE 330 115 75 128 93 aact:AA02077|3end_probable_outer_membrane_protein_1412708..1412858_POSITIVE 329 181 148 39 1 aact:AA00463|3end_class_B_acid_phosphatase_323898..324048_POSITIVE 328 187 141 118 65 aact:AA01185|3end_conserved_hypothetical_protein_(possible_membrane_protein)_813401..813551_POSITIVE 328 182 145 80 43 aact:AA00578|3end_DNA_topoisomerase_395201..395351_POSITIVE 324 180 132 148 96 aact:AA01520|3end_hypothetical_protein_1022887..1023037_POSITIVE 321 180 135 148 106 aact:AA01025|3end_hypothetical_protein_717769..717919_POSITIVE 320 180 137 44 1 aact:AA00348|3end_50S_ribosomal_protein_L1_233336..233486_POSITIVE 318 184 143 132 94 aact:AA01259|3end_protoporphyrinogen_oxidase_854943..855093_POSITIVE