Origin IGS: ggatttctcctaaacagtagttttatctcggtacataaatatctgaaaaacattcccctcttttgtaaagaggagttagggggggatttggttcaatcattcaaacgcttccgccaacgccgcaaccgtcgacaatggtcgcgccgttgtccatctatttttacccaggcgggatattgctatgggttgagatcgtccggatgttgtctaagcaccgtcgagaatcgtaatatttgacatattttgctttcctcaaatgaaataaaaagataccgcacttaaaaagtgcgatcgatttttgaaatgtttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaacatttcaaaaatcgatcgcactttttaagtgcggtatctttttatttcatttgaggaaagcaaaatatgtcaaatattacgattctcgacggtgcttagacaacatccggacgatctcaacccatagcaatatcccgcctgggtaaaaatagatggacaacggcgcgaccattgtcgacggttgcggcgttggcggaagcgtttgaatgattgaaccaaatccccccctaactcctctttacaaaagaggggaatgtttttcagatatttatgtaccgagataaaactactgtttaggagaaatcc Mask Tandem Repeat Region ================================================ ggatttctcctaaacagtagttttatctcggtacataaatatctgaaaaacattcccctcttttgtaaagaggagttagggggggatttggttcaatcattcaaacgcttccgccaacgccgcaaccgtcgacaatggtcgcgccgttgtccatctatttttacccaggcgggatattgctatgggttgagatcgtccggatgttgtctaagcaccgtcgagaatcgtaatatttgacatattttgctttcctcaaatgaaataaaaagataccgcacttaaaaagtgcgatcgatttttgaaatgtttta Find is-nt database================================================ Query_seq: AA02416:AA02417|AA02416:AA02417:conserved hypothetical protein:GTP-binding protein:->->:1659639..1659949 311 ggatttctcctaaacagtagttttatctcggtacataaatatctgaaaaacattcccctcttttgtaaagaggagttagggggggatttggttcaatcattcaaacgcttccgccaacgccgcaaccgtcgacaatggtcgcgccgttgtccatctatttttacccaggcgggatattgctatgggttgagatcgtccggatgttgtctaagcaccgtcgagaatcgtaatatttgacatattttgctttcctcaaatgaaataaaaagataccgcacttaaaaagtgcgatcgatttttgaaatgtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02416:AA02417|AA02416:AA02417:conserved hypothetical protein:GTP-binding protein:->->:1659639..1659949 311 ggatttctcctaaacagtagttttatctcggtacataaatatctgaaaaacattcccctcttttgtaaagaggagttagggggggatttggttcaatcattcaaacgcttccgccaacgccgcaaccgtcgacaatggtcgcgccgttgtccatctatttttacccaggcgggatattgctatgggttgagatcgtccggatgttgtctaagcaccgtcgagaatcgtaatatttgacatattttgctttcctcaaatgaaataaaaagataccgcacttaaaaagtgcgatcgatttttgaaatgtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02416:AA02417|AA02416:AA02417:conserved hypothetical protein:GTP-binding protein:->->:1659639..1659949 311 ggatttctcctaaacagtagttttatctcggtacataaatatctgaaaaacattcccctcttttgtaaagaggagttagggggggatttggttcaatcattcaaacgcttccgccaacgccgcaaccgtcgacaatggtcgcgccgttgtccatctatttttacccaggcgggatattgctatgggttgagatcgtccggatgttgtctaagcaccgtcgagaatcgtaatatttgacatattttgctttcctcaaatgaaataaaaagataccgcacttaaaaagtgcgatcgatttttgaaatgtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ggatttctcctaaacagtagttttatctcggtacataaatatctgaaaaacattcccctcttttgtaaagaggagttagggggggatttggttcaatcattcaaacgcttccgccaacgccgcaaccgtcgacaatggtcgcgccgttgtccatctatttttacccaggcgggatattgctatgggttgagatcgtccggatgttgtctaagcaccgtcgagaatcgtaatatttgacatattttgctttcctcaaatgaaataaaaagataccgcacttaaaaagtgcgatcgatttttgaaatgtttta Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 86 End: 187 ..................................................................................... M W F N H S N A S A N A A T V D N G R A V V H L F L P R R D I A M G ............................................................................................................................ Protein_Len: 44 Strand: + Start: 96 End: 227 ............................................................................................... M I Q T L P P T P Q P S T M V A P L S I Y F Y P G G I L L W V E I V R M L S K H R R E S .................................................................................... Protein_Len: 60 Strand: + Start: 25 End: 234 ........................ M S V H K Y L K N I P L F C K E E L G G D L V Q S F K R F R Q R R N R R Q W S R R C P S I F T Q A G Y C Y G L R S S G C ............................................................................. Protein_Len: 32 Strand: - Start: 183 End: 278 ...................................................................................................................................................................................... P N L D D P H Q R L V T S F R L I Q C I K S E E F S I F L Y R M ................................. Protein_Len: 43 Strand: - Start: 164 End: 292 ................................................................................................................................................................... G P P I N S H T S I T R I N D L C R R S D Y Y K V Y K A K R L H F L F I G C K F L A M ................... ggatttctcctaaacagtagttttatctcggtacataaatatctgaaaaacattcccctcttttgtaaagaggagttagggggggatttggttcaatcattcaaacgcttccgccaacgccgcaaccgtcgacaatggtcgcgccgttgtccatctatttttacccaggcgggatattgctatgggttgagatcgtccggatgttgtctaagcaccgtcgagaatcgtaatatttgacatattttgctttcctcaaatgaaataaaaagataccgcacttaaaaagtgcgatcgatttttgaaatgtttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ggatttctcctaaacagtagttttatctcggtacataaatatctgaaaaacattcccctcttttgtaaagaggagttagggggggatttggttcaatcattcaaacgcttccgccaacgccgcaaccgtcgacaatggtcgcgccgttgtccatctatttttacccaggcgggatattgctatgggttgagatcgtccggatgttgtctaagcaccgtcgagaatcgtaatatttgacatattttgctttcctcaaatgaaataaaaagataccgcacttaaaaagtgcgatcgatttttgaaatgtttta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 63 HP_score: -6.6 Tail_Score: -3.44257 Start: 118 End: 146 Full_Region: AAACGCTTCCGCCAA CGCCGCAACCGT CGACA ATGGTCGCGCCG TTGTCCATCTATTTT .....................................................................................................................CGCCGCAACCGTCGACAATGGTCGCGCCG..................................................................................................................................................................... Find igs database================================================ Query_seq: AA02416:AA02417|AA02416:AA02417:conserved hypothetical protein:GTP-binding protein:->->:1659639..1659949 311 ggatttctcctaaacagtagttttatctcggtacataaatatctgaaaaacattcccctcttttgtaaagaggagttagggggggatttggttcaatcattcaaacgcttccgccaacgccgcaaccgtcgacaatggtcgcgccgttgtccatctatttttacccaggcgggatattgctatgggttgagatcgtccggatgttgtctaagcaccgtcgagaatcgtaatatttgacatattttgctttcctcaaatgaaataaaaagataccgcacttaaaaagtgcgatcgatttttgaaatgtttta Intra-Species Hit: Count: 1 Min: 1 Max: 311 Len: 311 Subject: aact_AA02416_AA02417|conserved hypothetical protein:GTP-binding protein|POSITIVE:POSITIVE|[1659639,1659949]|311 HSP 1 e-value: 1.0E-163 bit: 575.0 Len: 311 Query Start:1 Query End:311 Subject Strand: POSITIVE Subject Start: 1 Subject End: 311 ggatttctcctaaacagtagttttatctcggtacataaatatctgaaaaacattcccctcttttgtaaagaggagttannnnnnnatttggttcaatcattcaaacgcttccgccaacgccgcaaccgtcgacaatggtcgcgccgttgtccatctatttttacccaggcgggatattgctatgggttgagatcgtccggatgttgtctaagcaccgtcgagaatcgtaatatttgacatattttgctttcctcaaatgaaataaaaagataccgcacttaaaaagtgcgatcgatttttgaaatgtttta ggatttctcctaaacagtagttttatctcggtacataaatatctgaaaaacattcccctcttttgtaaagaggagttagggggggatttggttcaatcattcaaacgcttccgccaacgccgcaaccgtcgacaatggtcgcgccgttgtccatctatttttacccaggcgggatattgctatgggttgagatcgtccggatgttgtctaagcaccgtcgagaatcgtaatatttgacatattttgctttcctcaaatgaaataaaaagataccgcacttaaaaagtgcgatcgatttttgaaatgtttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGAUUUCUCCUAAACAGUAGUUUUAUCUCGGUACAUAAAUAUCUGAAAAACAUUCCCCUCUUUUGUAAAGAGGAGUUAGGGGGGGAUUUGGUUCAAUCAUUCAAACGCUUCCGCCAACGCCGCAACCGUCGACAAUGGUCGCGCCGUUGUCCAUCUAUUUUUACCCAGGCGGGAUAUUGCUAUGGGUUGAGAUCGUCCGGAUGUUGUCUAAGCACCGUCGAGAAUCGUAAUAUUUGACAUAUUUUGCUUUCCUCAAAUGAAAUAAAAAGAUACCGCACUUAAAAAGUGCGAUCGAUUUUUGAAAUGUUUUA ((((..(((((.(((....(((((.((..((((......)))).))))))).....((((((.....)))))).))).)))))..))))....(((.(((((..(((.......(((((.(((.(((((.....))))).))).))))).......(((((.((((((((((....))))).))))).))))))))..))))))))...(((((...((((((((((...(((((((...............)))))))..............((((((.....))))))..))))))))))..))))).. (-83.26) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAACAUUUCAAAAAUCGAUCGCACUUUUUAAGUGCGGUAUCUUUUUAUUUCAUUUGAGGAAAGCAAAAUAUGUCAAAUAUUACGAUUCUCGACGGUGCUUAGACAACAUCCGGACGAUCUCAACCCAUAGCAAUAUCCCGCCUGGGUAAAAAUAGAUGGACAACGGCGCGACCAUUGUCGACGGUUGCGGCGUUGGCGGAAGCGUUUGAAUGAUUGAACCAAAUCCCCCCCUAACUCCUCUUUACAAAAGAGGGGAAUGUUUUUCAGAUAUUUAUGUACCGAGAUAAAACUACUGUUUAGGAGAAAUCC ..(((((............((((((((.....))))))))....(((((((((..(((((((..(...(((((.....))))).)..))))))).(((((.((((.....((((..(.((((..(((((..((........)).)))))......)))).).(((((.(((((((.........))))))).))))).))))...(((((((.((((......)))).........((((((((.....)))))))).......))))))).)))).))))))))))))))....))))).(((....))) (-86.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 509 88 42 202 163 aact:AA00658|5end_hypothetical_protein_451618..451848_POSITIVE 452 154 111 203 161 aact:AA02575|5end_transmembrane_protein_1793749..1793979_POSITIVE 444 146 106 163 130 aact:AA00993|5end_possible_D-tyrosyl-tRNA(Tyr)_deacylase_694488..694718_POSITIVE 429 150 101 176 115 aact:AA02715|5end_paraquat-inducible_protein_pqiB_homolog_1897053..1897283_POSITIVE 425 160 111 59 5 aact:AA00452|5end_phosphomannomutase;_phosphoglucomutase_315288..315518_POSITIVE 418 144 101 48 3 aact:AA01009|5end_universal_stress_protein_A_704443..704673_POSITIVE 417 157 111 209 144 aact:AA02933|5end_conserved_hypothetical_protein_(HesA/MoeB/ThiF_family_protein)_2053467..2053697_POSITIVE 417 149 101 152 88 aact:AA01628|5end_thiamin-monophosphate_kinase_1100888..1101118_POSITIVE 417 147 106 183 142 aact:AA00612|5end_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosami_ne_417840..418070_POSITIVE 416 152 111 79 43 aact:AA00603|5end_undecaprenyl_pyrophosphate_synthetase_(UPPS)_410283..410513_POSITIVE 412 155 111 135 79 aact:AA01645|5end_ferric_transport_ABC-related_ATP-binding_protein_1114016..1114246_POSITIVE 412 149 106 70 5 aact:AA00162|5end_pilin_C_biogenesis_protein/protein_transport_protein_114786..115016_POSITIVE 411 148 106 188 142 aact:AA01152|5end_sec-independent_protein_translocase_protein_789364..789594_POSITIVE 411 149 107 109 59 aact:AA00989|5end_hypothetical_protein_692319..692549_POSITIVE 407 150 106 229 180 aact:AA00585|5end_glycerol-3-phosphate_regulon_repressor_399195..399425_POSITIVE 402 149 102 110 59 aact:AA00329|5end_molybdopterin-guanine_dinucleotide_biosynthesis_protein_222737..222967_POSITIVE 398 154 112 107 59 aact:AA00574|5end_regulatory_protein_392113..392343_POSITIVE 396 227 185 225 183 aact:AA02416|5end_conserved_hypothetical_protein_1658920..1659150_POSITIVE 396 146 101 78 16 aact:AA01861|5end_phosphomethylpyrimidine_kinase_1251806..1252036_POSITIVE 396 149 105 189 128 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 417 149 101 130 66 aact:AA01626|3end_N_utilization_substance_protein_B_1100946..1101096_POSITIVE 412 149 106 74 9 aact:AA00161|3end_pilin_B_biogenesis_protein;_fimbrial_transcription_regulation_114870..115020_POSITIVE 396 227 185 146 104 aact:AA02414|3end_hypothetical_protein_1658921..1659071_POSITIVE 396 146 101 82 20 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 389 170 126 52 5 aact:AA00451|3end_hypothetical_protein_315348..315498_POSITIVE 380 227 185 142 104 aact:AA01304|3end_penicillin-binding_protein_1A_885580..885730_POSITIVE 374 149 105 141 90 aact:AA00977|3end_transcriptional_regulator_(LysR_family)_683334..683484_POSITIVE 371 148 118 60 22 aact:AA00505|3end_hypothetical_protein_351733..351883_POSITIVE 370 150 107 122 71 aact:AA02228|3end_regulatory_protein,_sorC_family_1516011..1516161_POSITIVE 368 148 112 145 105 aact:AA01409|3end_lipoprotein_B_951049..951199_POSITIVE 362 145 101 49 1 aact:AA01422|3end_cell_division_protein_E,_ABC_system_ATP-binding_protein_956736..956886_POSITIVE 362 143 106 151 118 aact:AA00611|3end_(3R)-hydroxymyristoyl-acyl_carrier_protein_dehydrase_417896..418046_POSITIVE 361 143 109 141 107 aact:AA00508|3end_SAM-dependent_methyltransferase_353883..354033_POSITIVE 359 146 105 140 98 aact:AA00007|3end_superoxide_dismutase_[Cu-Zn]_precursor_5055..5205_POSITIVE 356 149 101 103 24 aact:AA00989|3end_hypothetical_protein_692644..692794_POSITIVE 355 150 105 148 90 aact:AA02175|3end_anthranilate_synthase_component_II_1488966..1489116_POSITIVE 355 151 112 81 44 aact:AA00474|3end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_331724..331874_POSITIVE 354 150 101 148 96 aact:AA02426|3end_uridylyltransferase_1674226..1674376_POSITIVE 353 141 101 73 29 aact:AA02480|3end_excinuclease_ABC_subunit_B_1718442..1718592_POSITIVE 352 149 104 145 78 aact:AA00205|3end_ribose_ABC_transporter_protein_/_high_affinity_D-ribose_transport_protein_142658..142808_POSITIVE