Origin IGS: tagcttttggttatatgatttataaaaaatagctattgttttatcggcggttctcatgctaaaactaacgccacttcattatttatcctcaggagaacatatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatatgttctcctgaggataaataatgaagtggcgttagttttagcatgagaaccgccgataaaacaatagctattttttataaatcatataaccaaaagcta Mask Tandem Repeat Region ================================================ tagcttttggttatatgatttataaaaaatagctattgttttatcggcggttctcatgctaaaactaacgccacttcattatttatcctcaggagaacatatt Find is-nt database================================================ Query_seq: AA02411:AA02412|AA02411:AA02412:cysteine synthetase:cysteine synthetase:->->:1657565..1657667 103 tagcttttggttatatgatttataaaaaatagctattgttttatcggcggttctcatgctaaaactaacgccacttcattatttatcctcaggagaacatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02411:AA02412|AA02411:AA02412:cysteine synthetase:cysteine synthetase:->->:1657565..1657667 103 tagcttttggttatatgatttataaaaaatagctattgttttatcggcggttctcatgctaaaactaacgccacttcattatttatcctcaggagaacatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02411:AA02412|AA02411:AA02412:cysteine synthetase:cysteine synthetase:->->:1657565..1657667 103 tagcttttggttatatgatttataaaaaatagctattgttttatcggcggttctcatgctaaaactaacgccacttcattatttatcctcaggagaacatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagcttttggttatatgatttataaaaaatagctattgttttatcggcggttctcatgctaaaactaacgccacttcattatttatcctcaggagaacatatt Predict ORF larger than 30AA ================================================ tagcttttggttatatgatttataaaaaatagctattgttttatcggcggttctcatgctaaaactaacgccacttcattatttatcctcaggagaacatatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagcttttggttatatgatttataaaaaatagctattgttttatcggcggttctcatgctaaaactaacgccacttcattatttatcctcaggagaacatatt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02411:AA02412|AA02411:AA02412:cysteine synthetase:cysteine synthetase:->->:1657565..1657667 103 tagcttttggttatatgatttataaaaaatagctattgttttatcggcggttctcatgctaaaactaacgccacttcattatttatcctcaggagaacatatt Intra-Species Hit: Count: 1 Min: 1 Max: 103 Len: 103 Subject: aact_AA02411_AA02412|cysteine synthetase:cysteine synthetase|POSITIVE:POSITIVE|[1657565,1657667]|103 HSP 1 e-value: 4.0E-52 bit: 204.0 Len: 103 Query Start:1 Query End:103 Subject Strand: POSITIVE Subject Start: 1 Subject End: 103 tagcttttggttatatgatttataaaaaatagctattgttttatcggcggttctcatgctaaaactaacgccacttcattatttatcctcaggagaacatatt tagcttttggttatatgatttataaaaaatagctattgttttatcggcggttctcatgctaaaactaacgccacttcattatttatcctcaggagaacatatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCUUUUGGUUAUAUGAUUUAUAAAAAAUAGCUAUUGUUUUAUCGGCGGUUCUCAUGCUAAAACUAACGCCACUUCAUUAUUUAUCCUCAGGAGAACAUAUU (((((.((..(((((.....)))))..)).))))).(((((....((((...................)))).............(((...)))))))).... (-15.71) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAUGUUCUCCUGAGGAUAAAUAAUGAAGUGGCGUUAGUUUUAGCAUGAGAACCGCCGAUAAAACAAUAGCUAUUUUUUAUAAAUCAUAUAACCAAAAGCUA .....((((((.((..((....(((((......)))))...))..)).))))))..............(((((.((..(((((.....)))))..)).))))) (-16.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 309 87 44 211 164 aact:AA01531|5end_hypothetical_protein_1028705..1028935_POSITIVE 306 74 35 215 173 aact:AA01522|5end_conserved_hypothetical_protein_1022840..1023070_POSITIVE 283 72 32 43 3 aact:AA01666|5end_penicillin-binding_protein_4;_D-alanyl-D-alanine_carboxypeptidase;_D-alanyl-D-alanine-endopeptidase_1128231..1128461_POSITIVE 283 77 32 179 138 aact:AA01143|5end_hypothetical_protein_783111..783341_POSITIVE 282 87 46 217 177 aact:AA00333|5end_1-phosphofructokinase;_tagatose-6-phosphate_kinase_225543..225773_POSITIVE 278 74 35 42 15 aact:AA00962|5end_exopolyphosphatase_(metaphosphatase)_671060..671290_POSITIVE 272 79 32 56 5 aact:AA00163|5end_type_IV_prepilin_peptidase;_type_II_secretion_pathway_related_protein_116006..116236_POSITIVE 268 76 38 102 50 aact:AA01437|5end_phosphoglycerate_kinase_964125..964355_POSITIVE 266 90 46 205 152 aact:AA00516|5end_autoinducer-2_production_protein_355931..356161_POSITIVE 259 77 33 46 13 aact:AA01755|5end_cobalt_membrane_transport_protein_1185825..1186055_POSITIVE 259 74 32 154 106 aact:AA00634|5end_DNA_polymerase_IV_(Pol_IV)_435182..435412_POSITIVE 255 70 27 195 143 aact:AA02886|5end_OBG-related_GTP-binding_protein_2023382..2023612_POSITIVE 254 72 31 202 155 aact:AA01922|5end_translation_initiation_factor_IF2_1294710..1294940_POSITIVE 252 78 31 113 63 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 250 79 44 45 3 aact:AA02044|5end_phosphotransferase_system_enzyme_II,_galactitol_specific,_protein_A_1391488..1391718_POSITIVE 247 79 43 54 9 aact:AA02692|5end_hydrogenase_4_subunit;_hydrogenase_3_subunit_1876324..1876554_POSITIVE 246 50 9 148 100 aact:AA00723|5end_peptidase_B_(leucyl_aminopeptidase)_496457..496687_POSITIVE 245 78 32 66 26 aact:AA02217|5end_deoxyribose-phosphate_aldolase_1509135..1509365_POSITIVE 245 78 45 71 33 aact:AA02099|5end_50S_ribosomal_protein_L25_1429639..1429869_POSITIVE 244 60 33 78 55 aact:AA01995|5end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355120..1355350_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 278 74 35 36 9 aact:AA00961|3end_conserved_hypothetical_protein_671134..671284_POSITIVE 272 79 32 56 5 aact:AA00162|3end_pilin_C_biogenesis_protein/protein_transport_protein_116086..116236_POSITIVE 269 79 32 64 7 aact:AA02043|3end_tagatose_6-phosphate_kinase_1391587..1391737_POSITIVE 259 77 33 43 10 aact:AA01753|3end_conserved_hypothetical_protein_1185902..1186052_POSITIVE 259 74 32 59 11 aact:AA00633|3end_hypothetical_protein_435167..435317_POSITIVE 246 50 9 133 85 aact:AA00722|3end_nucleoside_diphosphate_kinase_496522..496672_POSITIVE 244 60 33 129 106 aact:AA01994|3end_chorismate_mutase;_prephenate_dehydrogenase_1355251..1355401_POSITIVE 243 76 40 59 24 aact:AA00926|3end_CyaY_protein_635891..636041_POSITIVE 242 87 49 120 79 aact:AA02920|3end_hypothetical_protein_2045749..2045899_POSITIVE 240 89 45 79 29 aact:AA02389|3end_transcriptional_repressor_for_uxu_operon_1639360..1639510_POSITIVE 238 82 46 149 106 aact:AA02160|3end_conserved_hypothetical_protein_1478929..1479079_POSITIVE 238 79 31 141 80 aact:AA00515|3end_hypothetical_protein_355959..356109_POSITIVE 238 90 43 82 30 aact:AA00374|3end_short-chain_fatty_acids_transporter_253101..253251_POSITIVE 235 61 27 146 103 aact:AA02885|3end_conserved_hypothetical_protein_2023422..2023572_POSITIVE 233 77 32 83 32 aact:AA00758|3end_hypothetical_protein_520696..520846_POSITIVE 232 58 13 123 89 aact:AA02384|3end_2-dehydro-3-deoxygluconokinase_1633133..1633283_POSITIVE 229 82 45 140 98 aact:AA00611|3end_(3R)-hydroxymyristoyl-acyl_carrier_protein_dehydrase_417896..418046_POSITIVE 228 76 41 31 5 aact:AA00623|3end_hypothetical_protein_430632..430782_POSITIVE 225 70 21 71 20 aact:AA02902|3end_aerobic_respiration_control_protein_2031955..2032105_POSITIVE 223 75 31 125 78 aact:AA02566|3end_PTS_system,_IIC_component_1788179..1788329_POSITIVE