Origin IGS: taaggcgatcaaatccaaatcggcggcaacgacagctatctctctgtatgccgtttgcattataaggagagaatggcggagtagcggcggaaaagtgcggtcgttttttcaaatgttttttataaggacagacatcaagtctgtcctgttatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taataacaggacagacttgatgtctgtccttataaaaaacatttgaaaaaacgaccgcacttttccgccgctactccgccattctctccttataatgcaaacggcatacagagagatagctgtcgttgccgccgatttggatttgatcgcctta Mask Tandem Repeat Region ================================================ taaggcgatcaaatccaaatcggcggcaacgacagctatctctctgtatgccgtttgcattataaggagagaatggcggagtagcggcggaaaagtgcggtcgttttttcaaatgttttttataaggacagacatcaagtctgtcctgttatta Find is-nt database================================================ Query_seq: AA02407:AA02409|AA02407:AA02409:thymidine kinase:DNA ligase:->->:1653301..1653454 154 taaggcgatcaaatccaaatcggcggcaacgacagctatctctctgtatgccgtttgcattataaggagagaatggcggagtagcggcggaaaagtgcggtcgttttttcaaatgttttttataaggacagacatcaagtctgtcctgttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02407:AA02409|AA02407:AA02409:thymidine kinase:DNA ligase:->->:1653301..1653454 154 taaggcgatcaaatccaaatcggcggcaacgacagctatctctctgtatgccgtttgcattataaggagagaatggcggagtagcggcggaaaagtgcggtcgttttttcaaatgttttttataaggacagacatcaagtctgtcctgttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02407:AA02409|AA02407:AA02409:thymidine kinase:DNA ligase:->->:1653301..1653454 154 taaggcgatcaaatccaaatcggcggcaacgacagctatctctctgtatgccgtttgcattataaggagagaatggcggagtagcggcggaaaagtgcggtcgttttttcaaatgttttttataaggacagacatcaagtctgtcctgttatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaggcgatcaaatccaaatcggcggcaacgacagctatctctctgtatgccgtttgcattataaggagagaatggcggagtagcggcggaaaagtgcggtcgttttttcaaatgttttttataaggacagacatcaagtctgtcctgttatta Predict ORF larger than 30AA ================================================ Protein_Len: 49 Strand: + Start: 8 End: 154 ....... M K S K S A A T T A I S L Y A V C I I R R E W R S S G G K V R S F F Q M F F I R T D I K S V L L L Protein_Len: 37 Strand: - Start: 2 End: 112 . L R D F G F R R C R C S D R Q I G N A N Y P S F P P T A A S F H P R K K M .......................................... taaggcgatcaaatccaaatcggcggcaacgacagctatctctctgtatgccgtttgcattataaggagagaatggcggagtagcggcggaaaagtgcggtcgttttttcaaatgttttttataaggacagacatcaagtctgtcctgttatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaggcgatcaaatccaaatcggcggcaacgacagctatctctctgtatgccgtttgcattataaggagagaatggcggagtagcggcggaaaagtgcggtcgttttttcaaatgttttttataaggacagacatcaagtctgtcctgttatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02407:AA02409|AA02407:AA02409:thymidine kinase:DNA ligase:->->:1653301..1653454 154 taaggcgatcaaatccaaatcggcggcaacgacagctatctctctgtatgccgtttgcattataaggagagaatggcggagtagcggcggaaaagtgcggtcgttttttcaaatgttttttataaggacagacatcaagtctgtcctgttatta Intra-Species Hit: Count: 1 Min: 1 Max: 154 Len: 154 Subject: aact_AA02407_AA02409|thymidine kinase:DNA ligase|POSITIVE:POSITIVE|[1653301,1653454]|154 HSP 1 e-value: 2.0E-82 bit: 305.0 Len: 154 Query Start:1 Query End:154 Subject Strand: POSITIVE Subject Start: 1 Subject End: 154 taaggcgatcaaatccaaatcggcggcaacgacagctatctctctgtatgccgtttgcattataaggagagaatggcggagtagcggcggaaaagtgcggtcgttttttcaaatgttttttataaggacagacatcaagtctgtcctgttatta taaggcgatcaaatccaaatcggcggcaacgacagctatctctctgtatgccgtttgcattataaggagagaatggcggagtagcggcggaaaagtgcggtcgttttttcaaatgttttttataaggacagacatcaagtctgtcctgttatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGGCGAUCAAAUCCAAAUCGGCGGCAACGACAGCUAUCUCUCUGUAUGCCGUUUGCAUUAUAAGGAGAGAAUGGCGGAGUAGCGGCGGAAAAGUGCGGUCGUUUUUUCAAAUGUUUUUUAUAAGGACAGACAUCAAGUCUGUCCUGUUAUUA .(((((((((..((.....((.((.((.((..(.(((((((((((..((((.....)))).....)))))).))))))..)).)).)).))...))..))))))))).................(((((((((.....)))))))))....... (-51.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAACAGGACAGACUUGAUGUCUGUCCUUAUAAAAAACAUUUGAAAAAACGACCGCACUUUUCCGCCGCUACUCCGCCAUUCUCUCCUUAUAAUGCAAACGGCAUACAGAGAGAUAGCUGUCGUUGCCGCCGAUUUGGAUUUGAUCGCCUUA .......(((((((((.....)))))))))...................((((((.((...........((......))...((((((......((((.....))))...))))))..)).))))))...(.(((((.......))))).)... (-39.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 506 120 71 122 74 aact:AA00860|5end_phosphoheptose_isomerase_591866..592096_POSITIVE 490 120 71 231 175 aact:AA01609|5end_p-aminobenzoate_synthase_component_I_homolog_1087903..1088133_POSITIVE 452 120 73 138 74 aact:AA00378|5end_leucine-responsive_regulatory_protein_(transcription_regulator)_254324..254554_POSITIVE 447 109 72 192 153 aact:AA01810|5end_electron_transport_complex_protein_1219250..1219480_POSITIVE 446 110 66 89 49 aact:AA02533|5end_zinc_protease;_peptidase_1762010..1762240_POSITIVE 444 120 74 121 64 aact:AA00837|5end_cell_division_protein_W_576317..576547_POSITIVE 438 123 85 212 172 aact:AA02395|5end_ATP-dependent_proteinase_1644613..1644843_POSITIVE 431 128 86 135 89 aact:AA01286|5end_5,10-methylenetetrahydrofolate_dehydrogenase_(bifunctional_protein)_870309..870539_POSITIVE 429 100 51 116 67 aact:AA00142|5end_glycerate_kinase_101309..101539_POSITIVE 425 100 57 113 57 aact:AA00789|5end_DNA_topoisomerase_III_540486..540716_POSITIVE 423 120 71 122 68 aact:AA01744|5end_peptide_chain_release_factor_RF-2_1179373..1179603_POSITIVE 420 120 80 208 166 aact:AA01175|5end_6-phosphogluconate_dehydrogenase_804864..805094_POSITIVE 420 120 80 44 2 aact:AA01174|5end_hypothetical_protein_804700..804930_POSITIVE 420 105 72 66 31 aact:AA00747|5end_UTP--hexose-1-phosphate_uridylyltransferase_/_UDPglucose-hexose-1-phosphate_uridylyltransferas_512384..512614_POSITIVE 414 130 81 145 85 aact:AA01954|5end_excinuclease_ABC,_subunit_C_1320468..1320698_POSITIVE 413 127 81 152 109 aact:AA02885|5end_conserved_hypothetical_protein_2022428..2022658_POSITIVE 409 131 92 109 71 aact:AA00065|5end_hypothetical_protein_48854..49084_POSITIVE 408 114 72 74 32 aact:AA01271|5end_hypothetical_protein_863794..864024_POSITIVE 403 129 85 192 152 aact:AA02261|5end_30S_ribosomal_protein_S9_1538013..1538243_POSITIVE 403 100 51 205 144 aact:AA01987|5end_hypothetical_protein_1350415..1350645_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 444 120 74 77 20 aact:AA00835|3end_UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapep_tide)_576353..576503_POSITIVE 413 127 81 71 28 aact:AA02883|3end_conserved_hypothetical_protein_2022427..2022577_POSITIVE 409 131 92 121 83 aact:AA00064|3end_ribosomal_small_subunit_pseudouridine_synthase_48946..49096_POSITIVE 408 114 72 97 55 aact:AA01270|3end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863897..864047_POSITIVE 403 129 85 61 21 aact:AA02260|3end_pseudouridylate_synthase_I_1537962..1538112_POSITIVE 396 120 71 53 3 aact:AA00833|3end_UDP-N-acetylmuramate-alanine_ligase_575218..575368_POSITIVE 392 139 94 44 1 aact:AA01945|3end_selenophosphate_synthase_1316022..1316172_POSITIVE 388 120 86 35 1 aact:AA02697|3end_hydrogenase_3_maturation_protease_1880408..1880558_POSITIVE 386 120 78 78 38 aact:AA00221|3end_NADP-specific_glutamate_dehydrogenase_153865..154015_POSITIVE 384 108 61 83 28 aact:AA01517|3end_peptidyl-prolyl_cis-trans_isomerase;_macrophage_infectivity_protein_1021791..1021941_POSITIVE 384 109 66 66 27 aact:AA01463|3end_probable_transport_protein_984200..984350_POSITIVE 384 119 84 85 48 aact:AA01280|3end_groES_chaperonin_868530..868680_POSITIVE 380 118 73 89 42 aact:AA00383|3end_chloride_channel_protein_260182..260332_POSITIVE 379 109 61 48 2 aact:AA00141|3end_hypoxanthine_phosphoribosyl_transferase_(HPRT)_101313..101463_POSITIVE 376 107 61 52 4 aact:AA02146|3end_iron(III)_ABC_transporter,_ATP-binding_protein_1469477..1469627_POSITIVE 375 100 51 145 89 aact:AA02927|3end_hypothetical_protein_2048405..2048555_POSITIVE 375 100 51 87 31 aact:AA02926|3end_hypothetical_protein_2048347..2048497_POSITIVE 372 127 87 132 93 aact:AA01363|3end_hypothetical_protein_922737..922887_POSITIVE 369 129 85 73 28 aact:AA02899|3end_oligopeptide_ABC_transporter,_ATP-binding_protein_2031138..2031288_POSITIVE 367 109 68 66 27 aact:AA02616|3end_transcriptional_regulator_1824675..1824825_POSITIVE