Origin IGS: taaatttgcctgccacttgtttatccccggaaaactgattaaagtaaatgcaaacgtttacgcgagataacatcgcgtaattttgttattaaggagcaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttgctccttaataacaaaattacgcgatgttatctcgcgtaaacgtttgcatttactttaatcagttttccggggataaacaagtggcaggcaaattta Mask Tandem Repeat Region ================================================ taaatttgcctgccacttgtttatccccggaaaactgattaaagtaaatgcaaacgtttacgcgagataacatcgcgtaattttgttattaaggagcaaaa Find is-nt database================================================ Query_seq: AA02371:AA02374|AA02371:AA02374:oxygen-independent coproporphyrinogen III oxidase:ribose 5-phosphate isomerase A:->->:1625878..1625978 101 taaatttgcctgccacttgtttatccccggaaaactgattaaagtaaatgcaaacgtttacgcgagataacatcgcgtaattttgttattaaggagcaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02371:AA02374|AA02371:AA02374:oxygen-independent coproporphyrinogen III oxidase:ribose 5-phosphate isomerase A:->->:1625878..1625978 101 taaatttgcctgccacttgtttatccccggaaaactgattaaagtaaatgcaaacgtttacgcgagataacatcgcgtaattttgttattaaggagcaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02371:AA02374|AA02371:AA02374:oxygen-independent coproporphyrinogen III oxidase:ribose 5-phosphate isomerase A:->->:1625878..1625978 101 taaatttgcctgccacttgtttatccccggaaaactgattaaagtaaatgcaaacgtttacgcgagataacatcgcgtaattttgttattaaggagcaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaatttgcctgccacttgtttatccccggaaaactgattaaagtaaatgcaaacgtttacgcgagataacatcgcgtaattttgttattaaggagcaaaa Predict ORF larger than 30AA ================================================ taaatttgcctgccacttgtttatccccggaaaactgattaaagtaaatgcaaacgtttacgcgagataacatcgcgtaattttgttattaaggagcaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaatttgcctgccacttgtttatccccggaaaactgattaaagtaaatgcaaacgtttacgcgagataacatcgcgtaattttgttattaaggagcaaaa Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 86 HP_score: -7.8 Tail_Score: -4.89477 Start: 58 End: 80 Full_Region: AGTAAATGCAAACGT TTACGCGA GATAACA TCGCGTAA TTTTGTTATTAAGGA .........................................................TTACGCGAGATAACATCGCGTAA..................... Find igs database================================================ Query_seq: AA02371:AA02374|AA02371:AA02374:oxygen-independent coproporphyrinogen III oxidase:ribose 5-phosphate isomerase A:->->:1625878..1625978 101 taaatttgcctgccacttgtttatccccggaaaactgattaaagtaaatgcaaacgtttacgcgagataacatcgcgtaattttgttattaaggagcaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 101 Len: 101 Subject: aact_AA02371_AA02374|oxygen-independent coproporphyrinogen III oxidase:ribose 5-phosphate isomerase A|POSITIVE:POSITIVE|[1625878,1625978]|101 HSP 1 e-value: 6.0E-51 bit: 200.0 Len: 101 Query Start:1 Query End:101 Subject Strand: POSITIVE Subject Start: 1 Subject End: 101 taaatttgcctgccacttgtttatccccggaaaactgattaaagtaaatgcaaacgtttacgcgagataacatcgcgtaattttgttattaaggagcaaaa taaatttgcctgccacttgtttatccccggaaaactgattaaagtaaatgcaaacgtttacgcgagataacatcgcgtaattttgttattaaggagcaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUUUGCCUGCCACUUGUUUAUCCCCGGAAAACUGAUUAAAGUAAAUGCAAACGUUUACGCGAGAUAACAUCGCGUAAUUUUGUUAUUAAGGAGCAAAA ....(((((...((....((((.((....))))))..............(((((...((((((((.......)))))))).)))))......)).))))). (-21.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUGCUCCUUAAUAACAAAAUUACGCGAUGUUAUCUCGCGUAAACGUUUGCAUUUACUUUAAUCAGUUUUCCGGGGAUAAACAAGUGGCAGGCAAAUUUA .((((((((.......((((.((((((((.......))))))))...))))...............((((((....)).))))....))..)))))).... (-20.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 302 45 7 63 21 aact:AA01956|5end_3-deoxy-manno-octulosonate_cytidylyltransferase_1322436..1322666_POSITIVE 296 97 53 55 6 aact:AA02052|5end_probable_exodeoxyribonuclease_VII_small_subunit_1398461..1398691_POSITIVE 272 93 55 47 8 aact:AA01981|5end_4-alpha-glucanotransferase_1337202..1337432_POSITIVE 258 78 35 196 141 aact:AA01998|5end_16S_rRNA_pseudouridine_synthase_1356842..1357072_POSITIVE 257 43 5 168 131 aact:AA01681|5end_molybdate-binding_periplasmic_protein_1135159..1135389_POSITIVE 255 69 21 96 36 aact:AA02524|5end_orotidine_5'-phosphate_decarboxylase_1753313..1753543_POSITIVE 248 80 34 229 181 aact:AA01123|5end_possible_hemolysin;_membrane_protein_770318..770548_POSITIVE 244 50 7 92 43 aact:AA00164|5end_dephospho-CoA_kinase_(dephosphocoenzyme_A_kinase)_116745..116975_POSITIVE 242 39 11 183 157 aact:AA00892|5end_selenocysteine-specific_elongation_factor_(SelB_translation_factor)_618023..618253_POSITIVE 239 67 24 146 100 aact:AA01462|5end_xylose_operon_regulatory_protein_981530..981760_POSITIVE 238 81 60 44 25 aact:AA02395|5end_ATP-dependent_proteinase_1644613..1644843_POSITIVE 237 76 35 142 106 aact:AA00974|5end_glucosamine-fructose-6-phosphate_679215..679445_POSITIVE 236 69 23 59 16 aact:AA02226|5end_ABC_transporter_ATP-binding_protein_1514502..1514732_POSITIVE 235 64 21 81 36 aact:AA01932|5end_tRNA_pseudouridine_synthase_B_1305098..1305328_POSITIVE 234 60 12 135 90 aact:AA00240|5end_hypothetical_protein_171390..171620_POSITIVE 233 87 49 66 31 aact:AA00387|5end_transcription_regulatory_protein_262305..262535_POSITIVE 232 77 50 169 133 aact:AA00488|5end_purine_nucleoside_phosphorylase_338075..338305_POSITIVE 231 60 17 201 161 aact:AA02220|5end_ABC_transport_system_permease_protein_1511155..1511385_POSITIVE 230 32 7 145 119 aact:AA01823|5end_hypothetical_protein_1227563..1227793_POSITIVE 226 87 46 102 49 aact:AA01851|5end_hypothetical_protein_1244723..1244953_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 258 78 35 138 83 aact:AA01997|3end_probable_translation_factor_1356864..1357014_POSITIVE 255 69 21 87 27 aact:AA02523|3end_conserved_hypothetical_protein_1753384..1753534_POSITIVE 251 56 12 123 79 aact:AA02695|3end_hydrogenase-3_component_G_1879418..1879568_POSITIVE 248 80 34 151 103 aact:AA01120|3end_conserved_hypothetical_protein_770320..770470_POSITIVE 242 39 11 134 108 aact:AA00890|3end_conserved_hypothetical_protein_618054..618204_POSITIVE 240 97 60 87 51 aact:AA01611|3end_anthranilate_synthase_component_II_1089619..1089769_POSITIVE 239 67 24 132 86 aact:AA01460|3end_hypothetical_protein_981596..981746_POSITIVE 237 76 35 84 48 aact:AA00973|3end_transcriptional_regulator,_DeoR_family_679237..679387_POSITIVE 236 69 23 69 26 aact:AA02225|3end_ABC_transporter_ATP-binding_protein_1514592..1514742_POSITIVE 235 64 21 78 33 aact:AA01930|3end_ribosome-binding_factor_A_1305175..1305325_POSITIVE 234 60 12 83 38 aact:AA00239|3end_hypothetical_protein_171418..171568_POSITIVE 232 77 50 40 4 aact:AA00486|3end_nucleoside_transporter_338026..338176_POSITIVE 228 88 50 108 79 aact:AA02584|3end_ADP-heptose_synthase_1798729..1798879_POSITIVE 225 77 50 111 80 aact:AA02271|3end_hypothetical_protein_1548801..1548951_POSITIVE 225 85 47 77 44 aact:AA00283|3end_glutamate_5-kinase_(gamma-glutamyl_kinase)_200015..200165_POSITIVE 223 40 5 150 113 aact:AA00186|3end_o-succinylbenzoate-CoA_synthase;_OSB_synthase_132445..132595_POSITIVE 221 81 50 41 10 aact:AA02890|3end_hypothetical_protein_2025532..2025682_POSITIVE 221 68 27 69 40 aact:AA01359|3end_conserved_hypothetical_protein_921109..921259_POSITIVE 221 100 55 88 41 aact:AA01358|3end_conserved_hypothetical_protein_920387..920537_POSITIVE 220 100 56 146 94 aact:AA02897|3end_oligopeptide_ABC_transporter;_permease_protein_2029150..2029300_POSITIVE