Origin IGS: taattgattaaacatgaaaagggtatcttcagatgccttttttattgccgccaaaattacttggtataccagaaaattaattggtaaaaagtgggttaagaccttatagtacgaggcattaacccactttatgcctca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaggcataaagtgggttaatgcctcgtactataaggtcttaacccactttttaccaattaattttctggtataccaagtaattttggcggcaataaaaaaggcatctgaagatacccttttcatgtttaatcaatta Mask Tandem Repeat Region ================================================ taattgattaaacatgaaaagggtatcttcagatgccttttttattgccgccaaaattacttggtataccagaaaattaattggtaaaaagtgggttaagaccttatagtacgaggcattaacccactttatgcctca Find is-nt database================================================ Query_seq: AA02365:AA02366|AA02365:AA02366:hflC protein:hypothetical protein:->->:1623022..1623159 138 taattgattaaacatgaaaagggtatcttcagatgccttttttattgccgccaaaattacttggtataccagaaaattaattggtaaaaagtgggttaagaccttatagtacgaggcattaacccactttatgcctca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02365:AA02366|AA02365:AA02366:hflC protein:hypothetical protein:->->:1623022..1623159 138 taattgattaaacatgaaaagggtatcttcagatgccttttttattgccgccaaaattacttggtataccagaaaattaattggtaaaaagtgggttaagaccttatagtacgaggcattaacccactttatgcctca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02365:AA02366|AA02365:AA02366:hflC protein:hypothetical protein:->->:1623022..1623159 138 taattgattaaacatgaaaagggtatcttcagatgccttttttattgccgccaaaattacttggtataccagaaaattaattggtaaaaagtgggttaagaccttatagtacgaggcattaacccactttatgcctca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattgattaaacatgaaaagggtatcttcagatgccttttttattgccgccaaaattacttggtataccagaaaattaattggtaaaaagtgggttaagaccttatagtacgaggcattaacccactttatgcctca Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 14 End: 106 ............. M K R V S S D A F F I A A K I T W Y T R K L I G K K W V K T L ................................ Protein_Len: 44 Strand: - Start: 1 End: 132 L Q N F M F L T D E S A K K I A A L I V Q Y V L F N I P L F H T L V K Y Y S A N V W K M ...... taattgattaaacatgaaaagggtatcttcagatgccttttttattgccgccaaaattacttggtataccagaaaattaattggtaaaaagtgggttaagaccttatagtacgaggcattaacccactttatgcctca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattgattaaacatgaaaagggtatcttcagatgccttttttattgccgccaaaattacttggtataccagaaaattaattggtaaaaagtgggttaagaccttatagtacgaggcattaacccactttatgcctca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02365:AA02366|AA02365:AA02366:hflC protein:hypothetical protein:->->:1623022..1623159 138 taattgattaaacatgaaaagggtatcttcagatgccttttttattgccgccaaaattacttggtataccagaaaattaattggtaaaaagtgggttaagaccttatagtacgaggcattaacccactttatgcctca Intra-Species Hit: Count: 1 Min: 1 Max: 138 Len: 138 Subject: aact_AA02365_AA02366|hflC protein:hypothetical protein|POSITIVE:POSITIVE|[1623022,1623159]|138 HSP 1 e-value: 7.0E-73 bit: 274.0 Len: 138 Query Start:1 Query End:138 Subject Strand: POSITIVE Subject Start: 1 Subject End: 138 taattgattaaacatgaaaagggtatcttcagatgccttttttattgccgccaaaattacttggtataccagaaaattaattggtaaaaagtgggttaagaccttatagtacgaggcattaacccactttatgcctca taattgattaaacatgaaaagggtatcttcagatgccttttttattgccgccaaaattacttggtataccagaaaattaattggtaaaaagtgggttaagaccttatagtacgaggcattaacccactttatgcctca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUGAUUAAACAUGAAAAGGGUAUCUUCAGAUGCCUUUUUUAUUGCCGCCAAAAUUACUUGGUAUACCAGAAAAUUAAUUGGUAAAAAGUGGGUUAAGACCUUAUAGUACGAGGCAUUAACCCACUUUAUGCCUCA .((((((((....((((((((((((((....))))))))))))))....(((((......))))).........))))))))((((.((((((((((((..((((.......))))..)))))))))))).))))... (-49.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAGGCAUAAAGUGGGUUAAUGCCUCGUACUAUAAGGUCUUAACCCACUUUUUACCAAUUAAUUUUCUGGUAUACCAAGUAAUUUUGGCGGCAAUAAAAAAGGCAUCUGAAGAUACCCUUUUCAUGUUUAAUCAAUUA (((.((..((((((((((((.((((..(...)..)))).)))))))))))).(((((..........)))))..(((((....)))))..))........((((((..((((.....))))..))))))..))).... (-34.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 455 110 63 228 182 aact:AA00591|5end_ABC_transporter,_ATP-binding_protein_403613..403843_POSITIVE 368 129 83 156 103 aact:AA01984|5end_tellurite_resistance_protein;_hemagglutinin_homolog_1344817..1345047_POSITIVE 295 65 21 155 121 aact:AA00325|5end_conserved_hypothetical_protein_221314..221544_POSITIVE 275 70 22 156 109 aact:AA01813|5end_electron_transport_complex_protein_1220949..1221179_POSITIVE 273 41 19 229 206 aact:AA01015|5end_met_repressor_710511..710741_POSITIVE 265 53 20 90 54 aact:AA01347|5end_conserved_hypothetical_protein_913209..913439_POSITIVE 261 130 83 224 179 aact:AA02476|5end_conserved_hypothetical_protein_1711610..1711840_POSITIVE 261 130 83 94 49 aact:AA02475|5end_hypothetical_protein_1711480..1711710_POSITIVE 261 48 8 140 101 aact:AA02343|5end_glutamine_synthetase_1600173..1600403_POSITIVE 258 50 8 160 116 aact:AA02266|5end_exodeoxyribonuclease_V_beta_chain_1541680..1541910_POSITIVE 257 85 41 229 178 aact:AA02275|5end_conserved_hypothetical_protein_1549729..1549959_POSITIVE 257 85 41 112 61 aact:AA02274|5end_hypothetical_protein_1549612..1549842_POSITIVE 256 127 93 140 106 aact:AA00855|5end_arginine_ABC_transporter,_permease_protein_589584..589814_POSITIVE 256 54 13 208 159 aact:AA00460|5end_ATP-dependent_ClpY-related_protease;_heat_shock_protein_321023..321253_POSITIVE 251 55 21 136 98 aact:AA02735|5end_DNA_repair_protein_1915974..1916204_POSITIVE 249 130 82 141 96 aact:AA00126|5end_conserved_hypothetical_protein_91053..91283_POSITIVE 247 54 13 183 139 aact:AA02509|5end_chorismate_synthase_1740952..1741182_POSITIVE 247 50 14 53 24 aact:AA00241|5end_formate_acetyltransferase_activating_enzyme_(anaerobic_ribonucleoside-triphosphate_reductase_activating_protein)_171546..171776_POSITIVE 241 130 87 58 13 aact:AA00086|5end_2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxy_late_synthase_63437..63667_POSITIVE 240 65 21 171 127 aact:AA01115|5end_carboxylesterase_766545..766775_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 343 52 19 78 48 aact:AA01014|3end_UTP-glucose-1-phosphate_uridylyltransferase_710433..710583_POSITIVE 295 65 21 82 48 aact:AA00324|3end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_221321..221471_POSITIVE 288 45 14 77 46 aact:AA02365|3end_hflC_protein_1622961..1623111_POSITIVE 265 53 20 62 26 aact:AA01345|3end_conserved_hypothetical_protein_(possible_membrane_protein)_913261..913411_POSITIVE 261 130 83 82 37 aact:AA02474|3end_transmembrane_protein_1711548..1711698_POSITIVE 256 127 93 134 100 aact:AA00854|3end_arginine_transport_system_permease_protein_589658..589808_POSITIVE 241 48 10 38 1 aact:AA02342|3end_ISRSO11/IS150_related_transposase,_orfB_1600151..1600301_POSITIVE 241 130 87 64 19 aact:AA00085|3end_menaquinone-specific_isochorismate;_isochorismate_mutase_63523..63673_POSITIVE 240 65 21 54 10 aact:AA01114|3end_alcohol_dehydrogenase,_class_III;_glutathione-dependent_formaldehyde_dehydrogenase_766508..766658_POSITIVE 239 53 11 142 87 aact:AA01552|3end_hypothetical_protein_1039300..1039450_POSITIVE 238 67 21 148 93 aact:AA01825|3end_iron(III)_ABC_transporter,_permease_protein_1229493..1229643_POSITIVE 238 73 39 116 85 aact:AA00540|3end_hypothetical_protein_370261..370411_POSITIVE 237 41 4 60 20 aact:AA00931|3end_elongation_factor_Tu_(tufA)_643181..643331_POSITIVE 235 50 4 68 24 aact:AA02712|3end_tail_specific_protease_precursor_1894987..1895137_POSITIVE 234 52 21 50 12 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 230 70 21 130 87 aact:AA00452|3end_phosphomannomutase;_phosphoglucomutase_317023..317173_POSITIVE 229 129 82 106 50 aact:AA00486|3end_nucleoside_transporter_338026..338176_POSITIVE 227 66 21 119 66 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 226 50 14 124 91 aact:AA00781|3end_hypothetical_protein_538218..538368_POSITIVE 225 53 18 145 99 aact:AA01245|3end_tyrosine-specific_transport_protein_2_849681..849831_POSITIVE