Origin IGS: acataaccactcgaattgtagcatatacttttcagtattaacgccatctttcaaaatttaatacgctaatctctcccaataacattcaattcattaattttatttagaaacata .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tatgtttctaaataaaattaatgaattgaatgttattgggagagattagcgtattaaattttgaaagatggcgttaatactgaaaagtatatgctacaattcgagtggttatgt Mask Tandem Repeat Region ================================================ acataaccactcgaattgtagcatatacttttcagtattaacgccatctttcaaaatttaatacgctaatctctcccaataacattcaattcattaattttatttagaaacata Find is-nt database================================================ Query_seq: AA02355:AA02356|AA02355:AA02356:nicotinamide phosphoribosyl transferase:hypothetical protein:->->:1614474..1614587 114 acataaccactcgaattgtagcatatacttttcagtattaacgccatctttcaaaatttaatacgctaatctctcccaataacattcaattcattaattttatttagaaacata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02355:AA02356|AA02355:AA02356:nicotinamide phosphoribosyl transferase:hypothetical protein:->->:1614474..1614587 114 acataaccactcgaattgtagcatatacttttcagtattaacgccatctttcaaaatttaatacgctaatctctcccaataacattcaattcattaattttatttagaaacata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02355:AA02356|AA02355:AA02356:nicotinamide phosphoribosyl transferase:hypothetical protein:->->:1614474..1614587 114 acataaccactcgaattgtagcatatacttttcagtattaacgccatctttcaaaatttaatacgctaatctctcccaataacattcaattcattaattttatttagaaacata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acataaccactcgaattgtagcatatacttttcagtattaacgccatctttcaaaatttaatacgctaatctctcccaataacattcaattcattaattttatttagaaacata Predict ORF larger than 30AA ================================================ acataaccactcgaattgtagcatatacttttcagtattaacgccatctttcaaaatttaatacgctaatctctcccaataacattcaattcattaattttatttagaaacata Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acataaccactcgaattgtagcatatacttttcagtattaacgccatctttcaaaatttaatacgctaatctctcccaataacattcaattcattaattttatttagaaacata Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02355:AA02356|AA02355:AA02356:nicotinamide phosphoribosyl transferase:hypothetical protein:->->:1614474..1614587 114 acataaccactcgaattgtagcatatacttttcagtattaacgccatctttcaaaatttaatacgctaatctctcccaataacattcaattcattaattttatttagaaacata Intra-Species Hit: Count: 1 Min: 1 Max: 114 Len: 114 Subject: aact_AA02355_AA02356|nicotinamide phosphoribosyl transferase:hypothetical protein|POSITIVE:POSITIVE|[1614474,1614587]|114 HSP 1 e-value: 1.0E-58 bit: 226.0 Len: 114 Query Start:1 Query End:114 Subject Strand: POSITIVE Subject Start: 1 Subject End: 114 acataaccactcgaattgtagcatatacttttcagtattaacgccatctttcaaaatttaatacgctaatctctcccaataacattcaattcattaattttatttagaaacata acataaccactcgaattgtagcatatacttttcagtattaacgccatctttcaaaatttaatacgctaatctctcccaataacattcaattcattaattttatttagaaacata Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACAUAACCACUCGAAUUGUAGCAUAUACUUUUCAGUAUUAACGCCAUCUUUCAAAAUUUAAUACGCUAAUCUCUCCCAAUAACAUUCAAUUCAUUAAUUUUAUUUAGAAACAUA ............(((..(((.....)))..))).(((((((................))))))).................................................. ( -6.79) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUGUUUCUAAAUAAAAUUAAUGAAUUGAAUGUUAUUGGGAGAGAUUAGCGUAUUAAAUUUUGAAAGAUGGCGUUAAUACUGAAAAGUAUAUGCUACAAUUCGAGUGGUUAUGU .((.(((((.(((((..((((....))))...))))).))))).))..(((((......((((((...((((((..(((((....)))))))))))...))))))....))))) (-21.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 276 75 35 100 54 aact:AA00759|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_I_520603..520833_POSITIVE 252 88 42 154 104 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 233 49 2 157 118 aact:AA00044|5end_formate_dependent_nitrite_reductase,_Fe-S_protein/_anaerobic_DMSO_reductase_B_35340..35570_POSITIVE 223 78 35 187 144 aact:AA01724|5end_valyl-tRNA_synthetase_1164446..1164676_POSITIVE 222 88 42 173 130 aact:AA01732|5end_multidrug_resistance_protein_A_1170677..1170907_POSITIVE 222 50 6 223 179 aact:AA01658|5end_maltose_operon_periplasmic_protein_precursor_1124104..1124334_POSITIVE 217 49 15 38 6 aact:AA00325|5end_conserved_hypothetical_protein_221314..221544_POSITIVE 216 106 61 218 167 aact:AA01053|5end_conserved_hypothetical_protein_733119..733349_POSITIVE 215 77 32 142 79 aact:AA01013|5end_phosphomannomutase_708029..708259_POSITIVE 214 46 7 226 180 aact:AA00290|5end_conserved_hypothetical_protein_201272..201502_POSITIVE 212 47 2 72 22 aact:AA01738|5end_enoyl-(acyl-carrier-protein)_reductase_1174699..1174929_POSITIVE 207 45 6 151 108 aact:AA01276|5end_heptosyltransferase_III_864730..864960_POSITIVE 198 83 42 161 123 aact:AA01398|5end_KDO_kinase_944889..945119_POSITIVE 195 36 1 130 88 aact:AA01854|5end_conserved_hypothetical_protein_1246662..1246892_POSITIVE 195 48 1 198 144 aact:AA00479|5end_mannose-specific_phosphotransferase_system_IIC_333475..333705_POSITIVE 194 45 2 159 110 aact:AA01265|5end_hypothetical_protein_861979..862209_POSITIVE 194 90 41 138 83 aact:AA00882|5end_hypothetical_protein_613452..613682_POSITIVE 192 51 16 164 127 aact:AA00151|5end_polynucleotide_phosphorylase_108865..109095_POSITIVE 191 73 35 51 15 aact:AA01006|5end_conserved_hypothetical_protein_(possible_cytosine_deaminas)_702380..702610_POSITIVE 190 45 7 183 151 aact:AA02826|5end_conserved_hypothetical_protein_1986336..1986566_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 276 75 35 113 67 aact:AA00758|3end_hypothetical_protein_520696..520846_POSITIVE 235 105 62 128 89 aact:AA02030|3end_threonyl-tRNA_synthetase_1384179..1384329_POSITIVE 222 88 42 91 48 aact:AA01731|3end_multidrug_resistance_protein_B_1170675..1170825_POSITIVE 222 50 6 134 90 aact:AA01657|3end_maltose_high-affinity_receptor_1124095..1124245_POSITIVE 222 98 63 142 100 aact:AA00314|3end_conserved_hypothetical_protein_(possible_Kmt1_protein)_214326..214476_POSITIVE 215 77 32 107 44 aact:AA01012|3end_probable_carbon_storage_regulator_homolog_708074..708224_POSITIVE 209 79 32 80 47 aact:AA01666|3end_penicillin-binding_protein_4;_D-alanyl-D-alanine_carboxypeptidase;_D-alanyl-D-alanine-endopeptidase_1129621..1129771_POSITIVE 208 50 8 46 14 aact:AA01903|3end_hypothetical_protein_1284215..1284365_POSITIVE 208 80 31 119 67 aact:AA01260|3end_hypothetical_protein_855758..855908_POSITIVE 198 83 42 72 34 aact:AA01396|3end_saccharide_biosynthesis_regulatory_protein_944880..945030_POSITIVE 194 90 41 91 36 aact:AA00881|3end_hypothetical_protein_613485..613635_POSITIVE 192 51 16 76 39 aact:AA00150|3end_conserved_hypothetical_protein_(possible_TPR_domain_protein)_108857..109007_POSITIVE 189 80 40 90 44 aact:AA01829|3end_hypothetical_protein_1231662..1231812_POSITIVE 188 67 21 41 2 aact:AA00981|3end_carboxylesterase_686377..686527_POSITIVE 186 46 7 41 2 aact:AA01810|3end_electron_transport_complex_protein_1220406..1220556_POSITIVE 185 79 32 121 88 aact:AA02144|3end_iron_(III)_ABC_transporter,_permease_protein_1468679..1468829_POSITIVE 185 87 42 119 68 aact:AA01843|3end_sensor_protein;_probable_two-component_system_sensor_histidine_kinase_1239830..1239980_POSITIVE 184 46 7 50 13 aact:AA01422|3end_cell_division_protein_E,_ABC_system_ATP-binding_protein_956736..956886_POSITIVE 182 52 12 110 72 aact:AA00665|3end_plasmid_protein_457550..457700_POSITIVE 180 78 35 116 64 aact:AA01566|3end_conserved_hypothetical_protein_1052091..1052241_POSITIVE