Origin IGS: tgaaagattgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttaacgtaatttgagtgtcattgggcgatagccatctcgttttctgttccttttataccgcaacaagatagtttcttttatctcacgataatcgtgtttaacttgctgataataaaagatagaacgaggcattttagccacacgaagtaagtcattcaacgcgtgatttggcttcaatctttca Mask Tandem Repeat Region ================================================ tgaaagattgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Find is-nt database================================================ Query_seq: AA02340:AA02341|AA02340:AA02341:hypothetical protein:hypothetical protein:->->:1599402..1599585 184 tgaaagattgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02340:AA02341|AA02340:AA02341:hypothetical protein:hypothetical protein:->->:1599402..1599585 184 tgaaagattgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02340:AA02341|AA02340:AA02341:hypothetical protein:hypothetical protein:->->:1599402..1599585 184 tgaaagattgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgaaagattgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Predict ORF larger than 30AA ================================================ Protein_Len: 59 Strand: + Start: 8 End: 181 ....... M K P N H A L N D L L R V A K M P R S I F Y Y Q Q V K H D Y R E I K E T I L L R Y K R N R K R D G Y R P M T L K L R * ... Protein_Len: 40 Strand: - Start: 38 End: 157 ..................................... K T H S F H R T R D K I I L L N F V I I T L Y F F S D Q Q P I F P V S F S I A M ........................... tgaaagattgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 83 Start: 25 End: 54 ........................GTTGAATGACTTACTTCGTGTGGCTAAAAT.................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgaaagattgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 72 HP_score: -7.7 Tail_Score: -3.79043 Start: 89 End: 101 Full_Region: tagtttcttttatct cacga taa tcgtg tttaacttgctgata ........................................................................................cacgataatcgtg................................................................................... Find igs database================================================ Query_seq: AA02340:AA02341|AA02340:AA02341:hypothetical protein:hypothetical protein:->->:1599402..1599585 184 tgaaagattgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Intra-Species Hit: Count: 4 Min: 1 Max: 184 Len: 184 Subject: aact_AA02340_AA02341|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1599402,1599585]|184 HSP 1 e-value: 1.0E-100 bit: 365.0 Len: 184 Query Start:1 Query End:184 Subject Strand: POSITIVE Subject Start: 1 Subject End: 184 tgaaagattgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa tgaaagattgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Subject: aact_AA02115_AA02116|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1445052,1445227]|176 HSP 1 e-value: 2.0E-95 bit: 349.0 Len: 176 Query Start:9 Query End:184 Subject Strand: POSITIVE Subject Start: 1 Subject End: 176 ........tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa ........tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Subject: aact_AA01288_AA01289|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[872349,872524]|176 HSP 1 e-value: 2.0E-95 bit: 349.0 Len: 176 Query Start:9 Query End:184 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 176 ........tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa ........tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Subject: aact_AA01075_AA01076|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[746129,746304]|176 HSP 1 e-value: 2.0E-95 bit: 349.0 Len: 176 Query Start:9 Query End:184 Subject Strand: POSITIVE Subject Start: 1 Subject End: 176 ........tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa ........tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAAGAUUGAAGCCAAAUCACGCGUUGAAUGACUUACUUCGUGUGGCUAAAAUGCCUCGUUCUAUCUUUUAUUAUCAGCAAGUUAAACACGAUUAUCGUGAGAUAAAAGAAACUAUCUUGUUGCGGUAUAAAAGGAACAGAAAACGAGAUGGCUAUCGCCCAAUGACACUCAAAUUACGUUAA .....(..((((((((..(((((((...............))))))).........((((((((.((((((((...(((((((.......(..(((((....)))))..).......)))))))....))))))))...)))..))))).))))).)))..)...................... (-40.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAACGUAAUUUGAGUGUCAUUGGGCGAUAGCCAUCUCGUUUUCUGUUCCUUUUAUACCGCAACAAGAUAGUUUCUUUUAUCUCACGAUAAUCGUGUUUAACUUGCUGAUAAUAAAAGAUAGAACGAGGCAUUUUAGCCACACGAAGUAAGUCAUUCAACGCGUGAUUUGGCUUCAAUCUUUCA .....((.....(((((((....(((....)))...((((..(((((..(((((((.(.((((..((((((......))))))((((.....))))......)))).)...))))))))))))))))))))))).....))..((((((((((((.......))))))).)))))......... (-41.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 940 60 11 143 94 aact:AA01289|5end_hypothetical_protein_872385..872615_POSITIVE 420 80 31 163 114 aact:AA00227|5end_hypothetical_sequence_156407..156637_POSITIVE 349 96 51 43 3 aact:AA00226|5end_hypothetical_protein_156276..156506_POSITIVE 333 65 22 210 152 aact:AA00207|5end_D-ribose_ABC_transporter,_ATP-binding_protein_/_galactoside_ABC_transporter,_ATP-binding_protein_142592..142822_POSITIVE 327 162 125 62 16 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 313 47 1 114 60 aact:AA01846|5end_conserved_hypothetical_protein_1241843..1242073_POSITIVE 313 169 123 149 95 aact:AA01411|5end_stationary-phase_survival_protein_951000..951230_POSITIVE 300 60 12 221 169 aact:AA00326|5end_periplasmic_oxidoreductase_(por)_(Thiol:disulfide_interchange_protein_dsbA_precursor.)_221703..221933_POSITIVE 289 46 13 46 5 aact:AA02564|5end_transketolase_N-terminal_section_1786173..1786403_POSITIVE 286 67 22 52 4 aact:AA02190|5end_hydrogenase-2_component_protein_1492919..1493149_POSITIVE 281 67 23 208 169 aact:AA02566|5end_PTS_system,_IIC_component_1787008..1787238_POSITIVE 280 60 14 216 168 aact:AA00795|5end_iron(III)_dicitrate-binding_periplasmic_protein_precursor_544793..545023_POSITIVE 280 167 124 226 178 aact:AA00013|5end_hypothetical_protein_6695..6925_POSITIVE 278 48 6 58 1 aact:AA02236|5end_stringent_starvation_protein_A_1521919..1522149_POSITIVE 270 178 134 123 81 aact:AA01279|5end_phage_exclusion-related_protein_867687..867917_POSITIVE 268 50 1 117 39 aact:AA00566|5end_30S_ribosomal_protein_S12_(streptomycin_resistance_protein)_390595..390825_POSITIVE 268 57 13 183 149 aact:AA00042|5end_hypothetical_protein_33810..34040_POSITIVE 267 49 12 71 41 aact:AA01231|5end_30S_ribosomal_protein_S14_840103..840333_POSITIVE 266 70 21 174 121 aact:AA02728|5end_conserved_hypothetical_protein_(possible_ATPase)_1910145..1910375_POSITIVE 266 67 23 79 33 aact:AA00464|5end_conserved_hypothetical_protein_323912..324142_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 933 170 121 76 27 aact:AA01288|3end_hypothetical_protein_872288..872438_POSITIVE 420 80 31 127 78 aact:AA00226|3end_hypothetical_protein_156451..156601_POSITIVE 313 169 123 118 64 aact:AA01409|3end_lipoprotein_B_951049..951199_POSITIVE 299 46 8 53 1 aact:AA02563|3end_transketolase_C-terminal_section_1786260..1786410_POSITIVE 280 60 14 143 95 aact:AA00794|3end_phosphohydrolase_544800..544950_POSITIVE 270 178 134 81 39 aact:AA01278|3end_hypothetical_protein_867725..867875_POSITIVE 268 57 13 110 76 aact:AA00041|3end_cytochrome_c-type_biogenesis_protein_33817..33967_POSITIVE 267 49 12 55 25 aact:AA01230|3end_50S_ribosomal_protein_L5_840167..840317_POSITIVE 267 180 145 122 80 aact:AA00977|3end_transcriptional_regulator_(LysR_family)_683334..683484_POSITIVE 263 48 4 145 100 aact:AA00806|3end_fumarate_reductase_15_kD_hydrophobic_protein_552834..552984_POSITIVE 263 49 12 151 106 aact:AA00325|3end_conserved_hypothetical_protein_221720..221870_POSITIVE 262 58 18 58 24 aact:AA01741|3end_6-pyruvoyl_tetrahydrobiopterin_synthase_1177695..1177845_POSITIVE 262 60 11 62 6 aact:AA01413|3end_conserved_hypothetical_protein_952860..953010_POSITIVE 259 57 13 135 93 aact:AA02564|3end_transketolase_N-terminal_section_1786783..1786933_POSITIVE 258 59 11 88 32 aact:AA02514|3end_esterase/lipase_1748378..1748528_POSITIVE 257 101 63 48 15 aact:AA00279|3end_8-amino-7-oxonoanoate_synthase_196514..196664_POSITIVE 254 59 14 149 108 aact:AA02177|3end_conserved_hypothetical_protein_1489432..1489582_POSITIVE 254 167 122 119 69 aact:AA00954|3end_uroporphyrinogen_III_synthase_661070..661220_POSITIVE 253 68 23 49 8 aact:AA02629|3end_hypothetical_protein_1830274..1830424_POSITIVE 253 70 21 113 69 aact:AA00665|3end_plasmid_protein_457550..457700_POSITIVE