Origin IGS: aatatctccttgtaaaaagaattgaaattttcaactattttagcattattcaaatttaatacaaaatccgaccgcacttttctttgtccactggtctgaacattacgcaaaatatgaaaagaggattgctccacattcccctgttttttagaatgatacgttttcctaagaaacgataaccaattcaataaaaggtacaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttgtaccttttattgaattggttatcgtttcttaggaaaacgtatcattctaaaaaacaggggaatgtggagcaatcctcttttcatattttgcgtaatgttcagaccagtggacaaagaaaagtgcggtcggattttgtattaaatttgaataatgctaaaatagttgaaaatttcaattctttttacaaggagatatt Mask Tandem Repeat Region ================================================ aatatctccttgtaaaaagaattgaaattttcaactattttagcattattcaaatttaatacaaaatccgaccgcacttttctttgtccactggtctgaacattacgcaaaatatgaaaagaggattgctccacattcccctgttttttagaatgatacgttttcctaagaaacgataaccaattcaataaaaggtacaaaa Find is-nt database================================================ Query_seq: AA02333:AA02334|AA02333:AA02334:conserved hypothetical protein:outer membrane protein P1:->->:1595245..1595446 202 aatatctccttgtaaaaagaattgaaattttcaactattttagcattattcaaatttaatacaaaatccgaccgcacttttctttgtccactggtctgaacattacgcaaaatatgaaaagaggattgctccacattcccctgttttttagaatgatacgttttcctaagaaacgataaccaattcaataaaaggtacaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02333:AA02334|AA02333:AA02334:conserved hypothetical protein:outer membrane protein P1:->->:1595245..1595446 202 aatatctccttgtaaaaagaattgaaattttcaactattttagcattattcaaatttaatacaaaatccgaccgcacttttctttgtccactggtctgaacattacgcaaaatatgaaaagaggattgctccacattcccctgttttttagaatgatacgttttcctaagaaacgataaccaattcaataaaaggtacaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02333:AA02334|AA02333:AA02334:conserved hypothetical protein:outer membrane protein P1:->->:1595245..1595446 202 aatatctccttgtaaaaagaattgaaattttcaactattttagcattattcaaatttaatacaaaatccgaccgcacttttctttgtccactggtctgaacattacgcaaaatatgaaaagaggattgctccacattcccctgttttttagaatgatacgttttcctaagaaacgataaccaattcaataaaaggtacaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatatctccttgtaaaaagaattgaaattttcaactattttagcattattcaaatttaatacaaaatccgaccgcacttttctttgtccactggtctgaacattacgcaaaatatgaaaagaggattgctccacattcccctgttttttagaatgatacgttttcctaagaaacgataaccaattcaataaaaggtacaaaa Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 59 End: 148 .......................................................... M Q N P T A L F F V H W S E H Y A K Y E K R I A P H S P V F ...................................................... Protein_Len: 37 Strand: + Start: 66 End: 176 ................................................................. M R P H F S L S T G L N I T Q N M K R G L L H I P L F F R M I R F P K K R .......................... Protein_Len: 42 Strand: - Start: 59 End: 184 .......................................................... Y L I R G C K E K D V P R F M V C F I F L P N S W M G R N K L I I R K G L F R Y G M .................. Protein_Len: 60 Strand: - Start: 3 End: 200 .. N F N E V I K A N N L N L V F D S R V K R Q G S T Q V N R L I H F S S Q E V N G Q K K S H Y T K R L F S L W N L L L Y M .. aatatctccttgtaaaaagaattgaaattttcaactattttagcattattcaaatttaatacaaaatccgaccgcacttttctttgtccactggtctgaacattacgcaaaatatgaaaagaggattgctccacattcccctgttttttagaatgatacgttttcctaagaaacgataaccaattcaataaaaggtacaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 82 Start: 125 End: 154 ............................................................................................................................ATTGCTCCACATTCCCCTGTTTTTTAGAAT................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatatctccttgtaaaaagaattgaaattttcaactattttagcattattcaaatttaatacaaaatccgaccgcacttttctttgtccactggtctgaacattacgcaaaatatgaaaagaggattgctccacattcccctgttttttagaatgatacgttttcctaagaaacgataaccaattcaataaaaggtacaaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02333:AA02334|AA02333:AA02334:conserved hypothetical protein:outer membrane protein P1:->->:1595245..1595446 202 aatatctccttgtaaaaagaattgaaattttcaactattttagcattattcaaatttaatacaaaatccgaccgcacttttctttgtccactggtctgaacattacgcaaaatatgaaaagaggattgctccacattcccctgttttttagaatgatacgttttcctaagaaacgataaccaattcaataaaaggtacaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 202 Len: 202 Subject: aact_AA02333_AA02334|conserved hypothetical protein:outer membrane protein P1|POSITIVE:POSITIVE|[1595245,1595446]|202 HSP 1 e-value: 1.0E-111 bit: 400.0 Len: 202 Query Start:1 Query End:202 Subject Strand: POSITIVE Subject Start: 1 Subject End: 202 aatatctccttgtaaaaagaattgaaattttcaactattttagcattattcaaatttaatacaaaatccgaccgcacttttctttgtccactggtctgaacattacgcaaaatatgaaaagaggattgctccacattcccctgttttttagaatgatacgttttcctaagaaacgataaccaattcaataaaaggtacaaaa aatatctccttgtaaaaagaattgaaattttcaactattttagcattattcaaatttaatacaaaatccgaccgcacttttctttgtccactggtctgaacattacgcaaaatatgaaaagaggattgctccacattcccctgttttttagaatgatacgttttcctaagaaacgataaccaattcaataaaaggtacaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAUCUCCUUGUAAAAAGAAUUGAAAUUUUCAACUAUUUUAGCAUUAUUCAAAUUUAAUACAAAAUCCGACCGCACUUUUCUUUGUCCACUGGUCUGAACAUUACGCAAAAUAUGAAAAGAGGAUUGCUCCACAUUCCCCUGUUUUUUAGAAUGAUACGUUUUCCUAAGAAACGAUAACCAAUUCAAUAAAAGGUACAAAA .......((((.......((((((...((((((..((((((.((.(((........))).......((.(((((.((........))....))))).)).......))))))))))))))..(((.((.....)).)))..((((((((((..............)))))))))).....)))))).....))))....... (-29.04) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUGUACCUUUUAUUGAAUUGGUUAUCGUUUCUUAGGAAAACGUAUCAUUCUAAAAAACAGGGGAAUGUGGAGCAAUCCUCUUUUCAUAUUUUGCGUAAUGUUCAGACCAGUGGACAAAGAAAAGUGCGGUCGGAUUUUGUAUUAAAUUUGAAUAAUGCUAAAAUAGUUGAAAAUUUCAAUUCUUUUUACAAGGAGAUAUU .......((((.((..(((((((.....((((.((((((..........)))))).)))).(((((.((.....)).))))).(((((((((((((((..((((((......)))))).............((((((((......))))))))...)))).))))))..)))))...)))))))....)).))))....... (-39.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 344 108 63 161 106 aact:AA01187|5end_conserved_hypothetical_protein_813331..813561_POSITIVE 341 108 69 70 31 aact:AA01880|5end_N-acetylglucosamine_repressor_1267083..1267313_POSITIVE 333 100 64 164 125 aact:AA01473|5end_type_I_restriction-modification_system_S_(specificity_subunit)_989025..989255_POSITIVE 330 95 53 225 178 aact:AA02191|5end_hypothetical_protein_1493318..1493548_POSITIVE 328 94 67 142 108 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 319 108 71 190 153 aact:AA02600|5end_conserved_hypothetical_protein_1810672..1810902_POSITIVE 316 110 64 174 136 aact:AA01648|5end_alpha-amylase_protein;_1,4-alpha-D-glucan_glucanohydrolase_1115170..1115400_POSITIVE 316 110 64 73 35 aact:AA01646|5end_hypothetical_protein_1115069..1115299_POSITIVE 316 110 68 64 27 aact:AA01140|5end_anaerobic_dimethyl_sulfoxide_reductase,_chain_C_781610..781840_POSITIVE 313 109 63 91 32 aact:AA02941|5end_hypothetical_protein_2058535..2058765_POSITIVE 309 92 63 92 62 aact:AA01986|5end_pyruvate_dehydrogenase_complex_E2_1348753..1348983_POSITIVE 308 109 64 173 141 aact:AA02860|5end_conserved_hypothetical_protein_2006074..2006304_POSITIVE 307 89 63 189 165 aact:AA02668|5end_gluconate_permease_1856758..1856988_POSITIVE 307 89 63 40 16 aact:AA02667|5end_hypothetical_protein_1856609..1856839_POSITIVE 305 110 62 111 59 aact:AA02569|5end_hypothetical_protein_1789012..1789242_POSITIVE 305 107 64 198 154 aact:AA01918|5end_anaerobic_C4-dicarboxylate_transporter_1290931..1291161_POSITIVE 303 118 71 62 12 aact:AA00459|5end_hypothetical_protein_320851..321081_POSITIVE 301 99 58 169 111 aact:AA02131|5end_conserved_hypothetical_protein_(possible_TrpH_protein)_1454239..1454469_POSITIVE 301 114 71 229 184 aact:AA00460|5end_ATP-dependent_ClpY-related_protease;_heat_shock_protein_321023..321253_POSITIVE 301 110 63 130 71 aact:AA00295|5end_hypothetical_protein_202267..202497_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 414 110 62 110 68 aact:AA02697|3end_hydrogenase_3_maturation_protease_1880408..1880558_POSITIVE 369 109 62 128 66 aact:AA02443|3end_cytochrome_C_related_protein_1686590..1686740_POSITIVE 344 108 63 151 96 aact:AA01185|3end_conserved_hypothetical_protein_(possible_membrane_protein)_813401..813551_POSITIVE 333 100 67 116 76 aact:AA02564|3end_transketolase_N-terminal_section_1786783..1786933_POSITIVE 333 100 64 61 22 aact:AA01471|3end_type_I_restriction_enzyme_M_(modification_chain)_989002..989152_POSITIVE 330 95 53 98 51 aact:AA02190|3end_hydrogenase-2_component_protein_1493271..1493421_POSITIVE 328 94 67 81 47 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 322 110 63 150 108 aact:AA01937|3end_sugar_fermentation_stimulation_protein_1309334..1309484_POSITIVE 316 110 64 108 70 aact:AA01645|3end_ferric_transport_ABC-related_ATP-binding_protein_1115184..1115334_POSITIVE 316 110 68 59 22 aact:AA01138|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_B_781685..781835_POSITIVE 313 109 63 128 69 aact:AA02940|3end_conserved_hypothetical_protein_2058652..2058802_POSITIVE 309 92 63 60 30 aact:AA01985|3end_pyruvate_dehydrogenase,_E1_component_1348801..1348951_POSITIVE 308 109 64 87 55 aact:AA02858|3end_conserved_hypothetical_protein_2006068..2006218_POSITIVE 302 95 62 147 115 aact:AA02597|3end_ribonuclease_D_1808358..1808508_POSITIVE 301 99 58 151 93 aact:AA02130|3end_hypothetical_protein_1454301..1454451_POSITIVE 301 110 63 121 62 aact:AA00292|3end_dihydrofolate_reductase_202338..202488_POSITIVE 300 98 71 146 118 aact:AA02599|3end_outer_membrane_protein,_Slp_family_1810717..1810867_POSITIVE 300 98 52 140 93 aact:AA02184|3end_anthranilate_isomerase;_indole-3-glycerol_phosphate_synthase;_phosphoribosylanthranilate_isomerase_1492541..1492691_POSITIVE 295 103 64 108 58 aact:AA00718|3end_ABC-related_ATP-binding_protein;_MRP_protein_494723..494873_POSITIVE 292 108 69 94 58 aact:AA01624|3end_glucose-6-phosphate_isomerase_1099901..1100051_POSITIVE