Origin IGS: aggaaaattaacaatgcaaatccgttccagtgtttctagctccgcccccttttaaaaagtaagaagccccaccacaatcaagattaaaacctgcagccataatatatgataattcagcccatgctttagcataattaggttccgatttagaatccggaggatcacataatcctgctttaagacattcttcacaacagtaattatgtggattgaaactatctgctccaccaaaaatcccatgcaaacattgattttttaactctttcataaacacctctacagtaaaaatctactctaaactagatgcacaaaacacctgcagggaaagataataatacatataattattcaaaagacttactttagcactgctttagaatgaaataacccgatagtaaaaatcctcaataaaagttaatttcatcgtaaatagaatacatcgagttataataaaaaaggcttaagttttcgacttaagccttttttatttatatcaatcaatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taattgattgatataaataaaaaaggcttaagtcgaaaacttaagccttttttattataactcgatgtattctatttacgatgaaattaacttttattgaggatttttactatcgggttatttcattctaaagcagtgctaaagtaagtcttttgaataattatatgtattattatctttccctgcaggtgttttgtgcatctagtttagagtagatttttactgtagaggtgtttatgaaagagttaaaaaatcaatgtttgcatgggatttttggtggagcagatagtttcaatccacataattactgttgtgaagaatgtcttaaagcaggattatgtgatcctccggattctaaatcggaacctaattatgctaaagcatgggctgaattatcatatattatggctgcaggttttaatcttgattgtggtggggcttcttactttttaaaagggggcggagctagaaacactggaacggatttgcattgttaattttcct Mask Tandem Repeat Region ================================================ aggaaaattaacaatgcaaatccgttccagtgtttctagctccgcccccttttaaaaagtaagaagccccaccacaatcaagattaaaacctgcagccataatatatgataattcagcccatgctttagcataattaggttccgatttagaatccggaggatcacataatcctgctttaagacattcttcacaacagtaattatgtggattgaaactatctgctccaccaaaaatcccatgcaaacattgattttttaactctttcataaacacctctacagtaaaaatctactctaaactagatgcacaaaacacctgcagggaaagataataatacatataattattcaaaagacttactttagcactgctttagaatgaaataacccgatagtaaaaatcctcaataaaagttaatttcatcgtaaatagaatacatcgagttataataaaaaaggcttaagttttcgacttaagccttttttatttatatcaatcaatta Find is-nt database================================================ Query_seq: AA02332:AA02333|AA02332:AA02333:conserved hypothetical protein; possible glycosyltransferase:conserved hypothetical protein:->->:1594453..1594956 504 aggaaaattaacaatgcaaatccgttccagtgtttctagctccgcccccttttaaaaagtaagaagccccaccacaatcaagattaaaacctgcagccataatatatgataattcagcccatgctttagcataattaggttccgatttagaatccggaggatcacataatcctgctttaagacattcttcacaacagtaattatgtggattgaaactatctgctccaccaaaaatcccatgcaaacattgattttttaactctttcataaacacctctacagtaaaaatctactctaaactagatgcacaaaacacctgcagggaaagataataatacatataattattcaaaagacttactttagcactgctttagaatgaaataacccgatagtaaaaatcctcaataaaagttaatttcatcgtaaatagaatacatcgagttataataaaaaaggcttaagttttcgacttaagccttttttatttatatcaatcaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02332:AA02333|AA02332:AA02333:conserved hypothetical protein; possible glycosyltransferase:conserved hypothetical protein:->->:1594453..1594956 504 aggaaaattaacaatgcaaatccgttccagtgtttctagctccgcccccttttaaaaagtaagaagccccaccacaatcaagattaaaacctgcagccataatatatgataattcagcccatgctttagcataattaggttccgatttagaatccggaggatcacataatcctgctttaagacattcttcacaacagtaattatgtggattgaaactatctgctccaccaaaaatcccatgcaaacattgattttttaactctttcataaacacctctacagtaaaaatctactctaaactagatgcacaaaacacctgcagggaaagataataatacatataattattcaaaagacttactttagcactgctttagaatgaaataacccgatagtaaaaatcctcaataaaagttaatttcatcgtaaatagaatacatcgagttataataaaaaaggcttaagttttcgacttaagccttttttatttatatcaatcaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02332:AA02333|AA02332:AA02333:conserved hypothetical protein; possible glycosyltransferase:conserved hypothetical protein:->->:1594453..1594956 504 aggaaaattaacaatgcaaatccgttccagtgtttctagctccgcccccttttaaaaagtaagaagccccaccacaatcaagattaaaacctgcagccataatatatgataattcagcccatgctttagcataattaggttccgatttagaatccggaggatcacataatcctgctttaagacattcttcacaacagtaattatgtggattgaaactatctgctccaccaaaaatcccatgcaaacattgattttttaactctttcataaacacctctacagtaaaaatctactctaaactagatgcacaaaacacctgcagggaaagataataatacatataattattcaaaagacttactttagcactgctttagaatgaaataacccgatagtaaaaatcctcaataaaagttaatttcatcgtaaatagaatacatcgagttataataaaaaaggcttaagttttcgacttaagccttttttatttatatcaatcaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aggaaaattaacaatgcaaatccgttccagtgtttctagctccgcccccttttaaaaagtaagaagccccaccacaatcaagattaaaacctgcagccataatatatgataattcagcccatgctttagcataattaggttccgatttagaatccggaggatcacataatcctgctttaagacattcttcacaacagtaattatgtggattgaaactatctgctccaccaaaaatcccatgcaaacattgattttttaactctttcataaacacctctacagtaaaaatctactctaaactagatgcacaaaacacctgcagggaaagataataatacatataattattcaaaagacttactttagcactgctttagaatgaaataacccgatagtaaaaatcctcaataaaagttaatttcatcgtaaatagaatacatcgagttataataaaaaaggcttaagttttcgacttaagccttttttatttatatcaatcaatta Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: - Start: 55 End: 252 ...................................................... V I L I L V Q L W L I H Y N L G H K L M I L N R N L I R L I V Y D Q K L V N K V V T I I H I S V I Q E V L F G M C V N M ............................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: - Start: 11 End: 289 .......... L E A W A K A Y N P E S K S D P P D C L G A K L C E E C C Y N H P N F S D A G G F I G H L C Q N K L E K M F V E V T F M ....................................................................................................................................................................................................................... aggaaaattaacaatgcaaatccgttccagtgtttctagctccgcccccttttaaaaagtaagaagccccaccacaatcaagattaaaacctgcagccataatatatgataattcagcccatgctttagcataattaggttccgatttagaatccggaggatcacataatcctgctttaagacattcttcacaacagtaattatgtggattgaaactatctgctccaccaaaaatcccatgcaaacattgattttttaactctttcataaacacctctacagtaaaaatctactctaaactagatgcacaaaacacctgcagggaaagataataatacatataattattcaaaagacttactttagcactgctttagaatgaaataacccgatagtaaaaatcctcaataaaagttaatttcatcgtaaatagaatacatcgagttataataaaaaaggcttaagttttcgacttaagccttttttatttatatcaatcaatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aggaaaattaacaatgcaaatccgttccagtgtttctagctccgcccccttttaaaaagtaagaagccccaccacaatcaagattaaaacctgcagccataatatatgataattcagcccatgctttagcataattaggttccgatttagaatccggaggatcacataatcctgctttaagacattcttcacaacagtaattatgtggattgaaactatctgctccaccaaaaatcccatgcaaacattgattttttaactctttcataaacacctctacagtaaaaatctactctaaactagatgcacaaaacacctgcagggaaagataataatacatataattattcaaaagacttactttagcactgctttagaatgaaataacccgatagtaaaaatcctcaataaaagttaatttcatcgtaaatagaatacatcgagttataataaaaaaggcttaagttttcgacttaagccttttttatttatatcaatcaatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 47 HP_score: -2.1 Tail_Score: -3.59247 Start: 156 End: 175 Full_Region: TCCGATTTAGAATCC GGAGGAT CACATA ATCCTGC TTTAAGACATTCTTC ...........................................................................................................................................................GGAGGATCACATAATCCTGC......................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -11.4 Tail_Score: -5.76237 Start: 458 End: 480 Full_Region: AGTTATAATAAAAAA GGCTTAAGTT TTC GACTTAAGCC TTTTTTATTTATATC .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................GGCTTAAGTTTTCGACTTAAGCC........................ Find igs database================================================ Query_seq: AA02332:AA02333|AA02332:AA02333:conserved hypothetical protein; possible glycosyltransferase:conserved hypothetical protein:->->:1594453..1594956 504 aggaaaattaacaatgcaaatccgttccagtgtttctagctccgcccccttttaaaaagtaagaagccccaccacaatcaagattaaaacctgcagccataatatatgataattcagcccatgctttagcataattaggttccgatttagaatccggaggatcacataatcctgctttaagacattcttcacaacagtaattatgtggattgaaactatctgctccaccaaaaatcccatgcaaacattgattttttaactctttcataaacacctctacagtaaaaatctactctaaactagatgcacaaaacacctgcagggaaagataataatacatataattattcaaaagacttactttagcactgctttagaatgaaataacccgatagtaaaaatcctcaataaaagttaatttcatcgtaaatagaatacatcgagttataataaaaaaggcttaagttttcgacttaagccttttttatttatatcaatcaatta Intra-Species Hit: Count: 1 Min: 1 Max: 504 Len: 504 Subject: aact_AA02332_AA02333|conserved hypothetical protein; possible glycosyltransferase:conserved hypothetical protein|POSITIVE:POSITIVE|[1594453,1594956]|504 HSP 1 e-value: 0.0 bit: 999.0 Len: 504 Query Start:1 Query End:504 Subject Strand: POSITIVE Subject Start: 1 Subject End: 504 aggaaaattaacaatgcaaatccgttccagtgtttctagctccgcccccttttaaaaagtaagaagccccaccacaatcaagattaaaacctgcagccataatatatgataattcagcccatgctttagcataattaggttccgatttagaatccggaggatcacataatcctgctttaagacattcttcacaacagtaattatgtggattgaaactatctgctccaccaaaaatcccatgcaaacattgattttttaactctttcataaacacctctacagtaaaaatctactctaaactagatgcacaaaacacctgcagggaaagataataatacatataattattcaaaagacttactttagcactgctttagaatgaaataacccgatagtaaaaatcctcaataaaagttaatttcatcgtaaatagaatacatcgagttataataaaaaaggcttaagttttcgacttaagccttttttatttatatcaatcaatta aggaaaattaacaatgcaaatccgttccagtgtttctagctccgcccccttttaaaaagtaagaagccccaccacaatcaagattaaaacctgcagccataatatatgataattcagcccatgctttagcataattaggttccgatttagaatccggaggatcacataatcctgctttaagacattcttcacaacagtaattatgtggattgaaactatctgctccaccaaaaatcccatgcaaacattgattttttaactctttcataaacacctctacagtaaaaatctactctaaactagatgcacaaaacacctgcagggaaagataataatacatataattattcaaaagacttactttagcactgctttagaatgaaataacccgatagtaaaaatcctcaataaaagttaatttcatcgtaaatagaatacatcgagttataataaaaaaggcttaagttttcgacttaagccttttttatttatatcaatcaatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGGAAAAUUAACAAUGCAAAUCCGUUCCAGUGUUUCUAGCUCCGCCCCCUUUUAAAAAGUAAGAAGCCCCACCACAAUCAAGAUUAAAACCUGCAGCCAUAAUAUAUGAUAAUUCAGCCCAUGCUUUAGCAUAAUUAGGUUCCGAUUUAGAAUCCGGAGGAUCACAUAAUCCUGCUUUAAGACAUUCUUCACAACAGUAAUUAUGUGGAUUGAAACUAUCUGCUCCACCAAAAAUCCCAUGCAAACAUUGAUUUUUUAACUCUUUCAUAAACACCUCUACAGUAAAAAUCUACUCUAAACUAGAUGCACAAAACACCUGCAGGGAAAGAUAAUAAUACAUAUAAUUAUUCAAAAGACUUACUUUAGCACUGCUUUAGAAUGAAAUAACCCGAUAGUAAAAAUCCUCAAUAAAAGUUAAUUUCAUCGUAAAUAGAAUACAUCGAGUUAUAAUAAAAAAGGCUUAAGUUUUCGACUUAAGCCUUUUUUAUUUAUAUCAAUCAAUUA ..((((.(((((.......((((..(((...((((((.(((................))).))))))..........((.(((((..((((((.............(((....)))....((((....))))...))))))..))))).))....))))))).(((((((.(((....(((.....))).....)))..)))))))(((((...((((((..............((((.((((......(((((((..(((...................))))))))))...((((...)))).............))))))))...................((((((.((((.(.............))))).))))))........))))))...))))).........)))))))))...................((.....(((((((((((((((((((...)))))))))))))))))))....))......... (-88.72) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUGAUUGAUAUAAAUAAAAAAGGCUUAAGUCGAAAACUUAAGCCUUUUUUAUUAUAACUCGAUGUAUUCUAUUUACGAUGAAAUUAACUUUUAUUGAGGAUUUUUACUAUCGGGUUAUUUCAUUCUAAAGCAGUGCUAAAGUAAGUCUUUUGAAUAAUUAUAUGUAUUAUUAUCUUUCCCUGCAGGUGUUUUGUGCAUCUAGUUUAGAGUAGAUUUUUACUGUAGAGGUGUUUAUGAAAGAGUUAAAAAAUCAAUGUUUGCAUGGGAUUUUUGGUGGAGCAGAUAGUUUCAAUCCACAUAAUUACUGUUGUGAAGAAUGUCUUAAAGCAGGAUUAUGUGAUCCUCCGGAUUCUAAAUCGGAACCUAAUUAUGCUAAAGCAUGGGCUGAAUUAUCAUAUAUUAUGGCUGCAGGUUUUAAUCUUGAUUGUGGUGGGGCUUCUUACUUUUUAAAAGGGGGCGGAGCUAGAAACACUGGAACGGAUUUGCAUUGUUAAUUUUCCU .....((.(((....((((((((((((((((((.....)))))))))))))))))).((((((((((((....(((..((((((((.....))))))))..)))...))).)))))))))..))).))...((((((((................((((((.......)))))).....(((...(.((((((((...(((((((((.(((.((((......((((((..(((..(((((.((((((.(((((((.(((....)))..)))))))((.(((...((.....))....(((((((((.(((((...(((.....)))..)))))))))))))).))).)).))))))...((((..(((....((((....)))))))))))....)))))...)))..))))))..)))).))).))))).)))).(((((((..((((....))))..).)))))).)))))))).)...)))...))))))))......... (-133.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 342 490 450 180 143 aact:AA02215|5end_conserved_hypothetical_protein_1508800..1509030_POSITIVE 306 70 22 230 180 aact:AA00979|5end_type_B_carboxylesterase;_p-nitrobenzyl_esterase_684645..684875_POSITIVE 304 75 43 177 146 aact:AA02731|5end_outer_membrane_lipoprotein_carrier_precursor_1911498..1911728_POSITIVE 303 45 1 85 40 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 303 324 289 136 99 aact:AA01018|5end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_711663..711893_POSITIVE 301 53 18 168 124 aact:AA00543|5end_ADP-heptose--LPS_heptosyltransferase_II_371287..371517_POSITIVE 296 159 116 59 19 aact:AA00823|5end_heat_shock_protein_565006..565236_POSITIVE 293 97 54 229 176 aact:AA01679|5end_aspartate-semialdehyde_dehydrogenase;_PTS_system_enzyme_II_A_component_1134356..1134586_POSITIVE 292 49 2 183 123 aact:AA00936|5end_ribose_ABC_transporter,_permease_protein,_C-terminal_646289..646519_POSITIVE 288 50 3 217 157 aact:AA01729|5end_hypothetical_protein_1168585..1168815_POSITIVE 287 160 112 74 20 aact:AA01845|5end_gamma-glutamyltranspeptidase_precursor_1239841..1240071_POSITIVE 286 47 2 80 22 aact:AA01451|5end_D-xylulokinase_971935..972165_POSITIVE 281 99 58 136 100 aact:AA00682|5end_S-adenosylmethionine-dependent_methyltransferase_466322..466552_POSITIVE 280 407 366 133 96 aact:AA00803|5end_hypothetical_protein_549526..549756_POSITIVE 277 45 1 228 181 aact:AA00884|5end_sodium-dependent_transporter_614392..614622_POSITIVE 277 45 1 134 87 aact:AA00883|5end_hypothetical_protein_614298..614528_POSITIVE 274 49 18 213 175 aact:AA00545|5end_glutathionylspermidine_synthetase;amidase_373607..373837_POSITIVE 273 69 24 216 154 aact:AA01032|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_B_720930..721160_POSITIVE 273 48 13 81 46 aact:AA00218|5end_argininosuccinate_lyase_150758..150988_POSITIVE 271 47 21 80 47 aact:AA02076|5end_conserved_hypothetical_protein_1409926..1410156_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 342 490 450 70 33 aact:AA02214|3end_pyruvate_kinase_II_1508770..1508920_POSITIVE 306 70 22 90 40 aact:AA00978|3end_conserved_hypothetical_protein_684585..684735_POSITIVE 303 324 289 119 82 aact:AA01017|3end_conserved_hypothetical_protein_711726..711876_POSITIVE 301 53 18 145 101 aact:AA00541|3end_conserved_hypothetical_protein_371344..371494_POSITIVE 293 47 1 128 80 aact:AA01062|3end_ribonuclease_PH_(RNase_PH)_736499..736649_POSITIVE 292 49 2 142 82 aact:AA00935|3end_ribose_ABC_transporter,_permease_protein,_N-terminal_646328..646478_POSITIVE 286 47 2 97 39 aact:AA01450|3end_hypothetical_protein_972032..972182_POSITIVE 280 407 366 66 29 aact:AA00801|3end_lysine--tRNA_ligase_549539..549689_POSITIVE 273 48 13 68 33 aact:AA00217|3end_hypothetical_protein_150825..150975_POSITIVE 271 47 21 53 20 aact:AA02075|3end_conserved_hypothetical_protein_1409979..1410129_POSITIVE 264 69 21 62 18 aact:AA01962|3end_recombination_protein_rec2_1328706..1328856_POSITIVE 256 223 187 139 98 aact:AA00592|3end_conserved_hypothetical_protein_(membrane/transport_protein)_407286..407436_POSITIVE 253 408 362 67 17 aact:AA00889|3end_conserved_hypothetical_protein_617110..617260_POSITIVE 253 160 113 128 67 aact:AA00790|3end_recombination_DNA_repair_protein_543134..543284_POSITIVE 252 198 152 119 62 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 251 60 15 42 2 aact:AA00663|3end_cystathionine_beta-lyase_455934..456084_POSITIVE 250 83 41 113 61 aact:AA03015|3end_hypothetical_protein_2105251..2105401_POSITIVE 250 487 443 78 36 aact:AA01368|3end_50S_ribosomal_protein_L19_924912..925062_POSITIVE 249 251 219 44 13 aact:AA02366|3end_hypothetical_protein_1623252..1623402_POSITIVE 247 47 2 132 80 aact:AA00506|3end_ATP-dependent_RNA_helicase_protein_353089..353239_POSITIVE