Origin IGS: taaattaaaattacccaagaaatctgtttataattgtttttatctttcgaattattattaattgtagtcggggctacaatattcatttagtagatataactttggaaagattgaaagctccctgtttatttttgtgttgggggataaatgaaaacacttaagcaaatttacagtgggtgggaatcgtagctgtaagaaatgggattattactggtacagttagttagaggagatttgccaattaaagcttctttgtatctcacattggaattttaatcattttgttgttctaaccaaaattactaaaaattatgcaactattcatgatcccacgataggtaaaagaaaattttctataaaacaattatccaattcatttacaggtgttactttagagatatggtctaaggttaaatttaagaaaggaaagtcagaaagtcagaacaaatcagcttatatgaaatcttaaaacatatgtcggaagttaaaattaaggtattttcactctctacactaattgagttagttggactattaatgctggtaggaacaaaactcgtaatggatcatgtaatgagctcgaaaaataaatcattactctttataatttgcttggggcttctcttattcacattttttcacttcatcagtcagtatgctaaaggcatggatttctttgaaagtggattatttaattaattttcaatagactgcaagtttctttttccacctattaaagttacaaaattaaac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gtttaattttgtaactttaataggtggaaaaagaaacttgcagtctattgaaaattaattaaataatccactttcaaagaaatccatgcctttagcatactgactgatgaagtgaaaaaatgtgaataagagaagccccaagcaaattataaagagtaatgatttatttttcgagctcattacatgatccattacgagttttgttcctaccagcattaatagtccaactaactcaattagtgtagagagtgaaaataccttaattttaacttccgacatatgttttaagatttcatataagctgatttgttctgactttctgactttcctttcttaaatttaaccttagaccatatctctaaagtaacacctgtaaatgaattggataattgttttatagaaaattttcttttacctatcgtgggatcatgaatagttgcataatttttagtaattttggttagaacaacaaaatgattaaaattccaatgtgagatacaaagaagctttaattggcaaatctcctctaactaactgtaccagtaataatcccatttcttacagctacgattcccacccactgtaaatttgcttaagtgttttcatttatcccccaacacaaaaataaacagggagctttcaatctttccaaagttatatctactaaatgaatattgtagccccgactacaattaataataattcgaaagataaaaacaattataaacagatttcttgggtaattttaattta Mask Tandem Repeat Region ================================================ taaattaaaattacccaagaaatctgtttataattgtttttatctttcgaattattattaattgtagtcggggctacaatattcatttagtagatataactttggaaagattgaaagctccctgtttatttttgtgttgggggataaatgaaaacacttaagcaaatttacagtgggtgggaatcgtagctgtaagaaatgggattattactggtacagttagttagaggagatttgccaattaaagcttctttgtatctcacattggaattttaatcattttgttgttctaaccaaaattactaaaaattatgcaactattcatgatcccacgataggtaaaagaaaattttctataaaacaattatccaattcatttacaggtgttactttagagatatggtctaaggttaaatttaagaaaggaaagtcagaaagtcagaacaaatcagcttatatgaaatcttaaaacatatgtcggaagttaaaattaaggtattttcactctctacactaattgagttagttggactattaatgctggtaggaacaaaactcgtaatggatcatgtaatgagctcgaaaaataaatcattactctttataatttgcttggggcttctcttattcacattttttcacttcatcagtcagtatgctaaaggcatggatttctttgaaagtggattatttaattaattttcaatagactgcaagtttctttttccacctattaaagttacaaaattaaac Find is-nt database================================================ Query_seq: AA02330:AA02331|AA02330:AA02331:UDP-N-acetylglucosamine enolpyruvyl transferase:ABC transporter, ATP-binding protein; possible leukotoxin secretion ATP-binding protein:->->:1591342..1592094 753 taaattaaaattacccaagaaatctgtttataattgtttttatctttcgaattattattaattgtagtcggggctacaatattcatttagtagatataactttggaaagattgaaagctccctgtttatttttgtgttgggggataaatgaaaacacttaagcaaatttacagtgggtgggaatcgtagctgtaagaaatgggattattactggtacagttagttagaggagatttgccaattaaagcttctttgtatctcacattggaattttaatcattttgttgttctaaccaaaattactaaaaattatgcaactattcatgatcccacgataggtaaaagaaaattttctataaaacaattatccaattcatttacaggtgttactttagagatatggtctaaggttaaatttaagaaaggaaagtcagaaagtcagaacaaatcagcttatatgaaatcttaaaacatatgtcggaagttaaaattaaggtattttcactctctacactaattgagttagttggactattaatgctggtaggaacaaaactcgtaatggatcatgtaatgagctcgaaaaataaatcattactctttataatttgcttggggcttctcttattcacattttttcacttcatcagtcagtatgctaaaggcatggatttctttgaaagtggattatttaattaattttcaatagactgcaagtttctttttccacctattaaagttacaaaattaaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02330:AA02331|AA02330:AA02331:UDP-N-acetylglucosamine enolpyruvyl transferase:ABC transporter, ATP-binding protein; possible leukotoxin secretion ATP-binding protein:->->:1591342..1592094 753 taaattaaaattacccaagaaatctgtttataattgtttttatctttcgaattattattaattgtagtcggggctacaatattcatttagtagatataactttggaaagattgaaagctccctgtttatttttgtgttgggggataaatgaaaacacttaagcaaatttacagtgggtgggaatcgtagctgtaagaaatgggattattactggtacagttagttagaggagatttgccaattaaagcttctttgtatctcacattggaattttaatcattttgttgttctaaccaaaattactaaaaattatgcaactattcatgatcccacgataggtaaaagaaaattttctataaaacaattatccaattcatttacaggtgttactttagagatatggtctaaggttaaatttaagaaaggaaagtcagaaagtcagaacaaatcagcttatatgaaatcttaaaacatatgtcggaagttaaaattaaggtattttcactctctacactaattgagttagttggactattaatgctggtaggaacaaaactcgtaatggatcatgtaatgagctcgaaaaataaatcattactctttataatttgcttggggcttctcttattcacattttttcacttcatcagtcagtatgctaaaggcatggatttctttgaaagtggattatttaattaattttcaatagactgcaagtttctttttccacctattaaagttacaaaattaaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 224 Max: 421 Len: 198 Subject: UniRef90_Q27T99 Cluster: CvaB, IS186 transposase; n=1; Escherichia coli|Rep: CvaB, IS186 transposase - Escherichia coli HSP 1 e-value: 7.0E-12 bit: 67.8 Len: 198 Query Start:224 Query End:421 Subject Strand: null Subject Start: 93 Subject End: 158 ............................................................................................................................................................................................................................... L E E I C Q L K L L C I S H W N F N H F V V L T K I T K N Y A T I H D P T I G K R K F S I K Q L S N S F T G V T L E I W S K V K F K ............................................................................................................................................................................................................................................................................................................................................ ............................................................................................................................................................................................................................... L D E L R V L K T P C I L H W D F S H F V V L V S V K R N R Y V L H D P A R G I R Y I S R E E M S R Y F T G V A L E V W P G S E F Q ............................................................................................................................................................................................................................................................................................................................................ Find nr database================================================ Query_seq: AA02330:AA02331|AA02330:AA02331:UDP-N-acetylglucosamine enolpyruvyl transferase:ABC transporter, ATP-binding protein; possible leukotoxin secretion ATP-binding protein:->->:1591342..1592094 753 taaattaaaattacccaagaaatctgtttataattgtttttatctttcgaattattattaattgtagtcggggctacaatattcatttagtagatataactttggaaagattgaaagctccctgtttatttttgtgttgggggataaatgaaaacacttaagcaaatttacagtgggtgggaatcgtagctgtaagaaatgggattattactggtacagttagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaaggaaagtcagaaagtcagaacaaatcagcttatatgaaatcttaaaacatatgtcggaagttaaaattaaggtattttcactctctacactaattgagttagttggactattaatgctggtaggaacaaaactcgtaatggatcatgtaatgagctcgaaaaataaatcattactctttataatttgcttggggcttctcttattcacattttttcacttcatcagtcagtatgctaaaggcatggatttctttgaaagtggattatttaattaattttcaatagactgcaagtttctttttccacctattaaagttacaaaattaaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 3 Min: 430 Max: 742 Len: 313 Subject: UniRef90_UPI00005F80BA Cluster: COG2274: ABC-type bacteriocin/lantibiotic exporters, contain an N-terminal double-glycine peptidase domain; n=1; Yersinia mollaretii ATCC 43969|Rep: COG2274: ABC-type bacteriocin/lantibiotic exporters, contain an N-terminal double-glycine peptidase domain - Yersinia mollaretii ATCC 43969 HSP 1 e-value: 2.0E-6 bit: 42.7 Len: 159 Query Start:430 Query End:588 Subject Strand: null Subject Start: 154 Subject End: 209 ............................................................................................................................................................................................................................................................................................................................................................................................................................................. V R K S E Q I S L Y E I L K H M S E V K - - - I K V F S L S T L I E L V G L L M L V G T K L V M D H V M S S K N ..................................................................................................................................................................... ............................................................................................................................................................................................................................................................................................................................................................................................................................................. V I Q Q S R L Q L R R L L S N I S G L K G A L T K I F C L S L I I E A V S L L M P V G T Q L V L D H V I Q A S D ..................................................................................................................................................................... HSP 2 e-value: 2.0E-6 bit: 32.3 Len: 126 Query Start:617 Query End:742 Subject Strand: null Subject Start: 219 Subject End: 260 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ G F S Y S H F F T S S V S M L K A W I S L K V D Y L I N F Q * T A S F F F H L L K L ........... ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ G L L F F I V F R T A V A M L R S W T T V V M D S L I D V Q W K A G M F G H L M A L ........... Subject: UniRef90_Q9EXN5 Cluster: Probable microcin H47 secretion/processing ATP-binding protein mchF; n=10; Enterobacteriaceae|Rep: Probable microcin H47 secretion/processing ATP-binding protein mchF - Escherichia coli HSP 1 e-value: 3.0E-6 bit: 38.9 Len: 147 Query Start:445 Query End:591 Subject Strand: null Subject Start: 159 Subject End: 210 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................ Q I S L Y E I L K H M S E V K - - - I K V F S L S T L I E L V G L L M L V G T K L V M D H V M S S K N K .................................................................................................................................................................. ............................................................................................................................................................................................................................................................................................................................................................................................................................................................ R I S L R S L I N S I Y G I K R T L A K I F C L S V V I E A I N L V M P V G T Q L V M D H A I P A G D R .................................................................................................................................................................. HSP 2 e-value: 3.0E-6 bit: 35.8 Len: 126 Query Start:617 Query End:742 Subject Strand: null Subject Start: 219 Subject End: 260 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ G F S Y S H F F T S S V S M L K A W I S L K V D Y L I N F Q * T A S F F F H L L K L ........... ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ G L M F F I L L R A A V S M L R A W S S L V M S T L I N I Q W Q S G L F N H L L R L ........... Subject: UniRef90_Q66G92 Cluster: ABC transporter, multidrug efflux pump, fused permease and ATPase domains; n=9; Yersinia|Rep: ABC transporter, multidrug efflux pump, fused permease and ATPase domains - Yersinia pseudotuberculosis HSP 1 e-value: 3.0E-6 bit: 40.4 Len: 144 Query Start:445 Query End:588 Subject Strand: null Subject Start: 159 Subject End: 209 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................ Q I S L Y E I L K H M S E V K - - - I K V F S L S T L I E L V G L L M L V G T K L V M D H V M S S K N ..................................................................................................................................................................... ............................................................................................................................................................................................................................................................................................................................................................................................................................................................ R L S L L S L M R N I S G L P G F L T K I F C L S L M V E A I N L L L P V G T Q L V M D H V I I A E D ..................................................................................................................................................................... HSP 2 e-value: 3.0E-6 bit: 33.9 Len: 126 Query Start:617 Query End:742 Subject Strand: null Subject Start: 219 Subject End: 260 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ G F S Y S H F F T S S V S M L K A W I S L K V D Y L I N F Q * T A S F F F H L L K L ........... ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ G L L F F I L F R T F L S M L R S W T S L V M G S L V D V Q W K A G L F D H L L K L ........... taaattaaaattacccaagaaatctgtttataattgtttttatctttcgaattattattaattgtagtcggggctacaatattcatttagtagatataactttggaaagattgaaagctccctgtttatttttgtgttgggggataaatgaaaacacttaagcaaatttacagtgggtgggaatcgtagctgtaagaaatgggattattactggtacagttagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaaggaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcattactctttataatttgcttggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncaaaattaaac Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 24 End: 158 ....................... M F I I V F I F R I I I N C S R G Y N I H L V D I T L E R L K A P C L F L C W G I N E N T ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 233 End: 466 ........................................................................................................................................................................................................................................ M C Q L K L L C I S H W N F N H F V V L T K I T K N Y A T I H D P T I G K R K F S I K Q L S N S F T G V T L E I W S K V ............................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 448 End: 696 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................... M S L Y E I L K H M S E V K I K V F S L S T L I E L V G L L M L V G T K L V M D H V M S S K N K S L L F I I C L G L L L ......................................................... Protein_Len: 48 Strand: - Start: 420 End: 563 ................................................................................................................................................................................................................................................................................................................................................................................................................................... S L F T L F D S C I L K Y S I K F C I D S T L I L T N E S E V S I S N T P S N I S T P V F S T M .............................................................................................................................................................................................. Protein_Len: 31 Strand: - Start: 171 End: 263 .......................................................................................................................................................................... L P H S D Y S Y S I P N N S T C N T L P S K G I L A E K Y R M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 35 Strand: - Start: 61 End: 165 ............................................................ N Y D P S C Y E N L L Y L K P F I S L E R N I K T N P S L H F C K L M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ taaattaaaattacccaagaaatctgtttataattgtttttatctttcgaattattattaattgtagtcggggctacaatattcatttagtagatataactttggaaagattgaaagctccctgtttatttttgtgttgggggataaatgaaaacacttaagcaaatttacagtgggtgggaatcgtagctgtaagaaatgggattattactggtacagttagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaaggaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcattactctttataatttgcttggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncaaaattaaac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 87 Start: 579 End: 608 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CTCGAAAAATAAATCATTACTCTTTATAAT................................................................................................................................................. PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 51 End: 80 ..................................................ATTATTATTAATTGTAGTCGGGGCTACAAT................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaattaaaattacccaagaaatctgtttataattgtttttatctttcgaattattattaattgtagtcggggctacaatattcatttagtagatataactttggaaagattgaaagctccctgtttatttttgtgttgggggataaatgaaaacacttaagcaaatttacagtgggtgggaatcgtagctgtaagaaatgggattattactggtacagttagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaaggaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcattactctttataatttgcttggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncaaaattaaac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02330:AA02331|AA02330:AA02331:UDP-N-acetylglucosamine enolpyruvyl transferase:ABC transporter, ATP-binding protein; possible leukotoxin secretion ATP-binding protein:->->:1591342..1592094 753 taaattaaaattacccaagaaatctgtttataattgtttttatctttcgaattattattaattgtagtcggggctacaatattcatttagtagatataactttggaaagattgaaagctccctgtttatttttgtgttgggggataaatgaaaacacttaagcaaatttacagtgggtgggaatcgtagctgtaagaaatgggattattactggtacagttagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaaggaaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntcattactctttataatttgcttggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncaaaattaaac Intra-Species Hit: Count: 1 Min: 1 Max: 222 Len: 222 Subject: aact_AA02330_AA02331|UDP-N-acetylglucosamine enolpyruvyl transferase:ABC transporter, ATP-binding protein; possible leukotoxin secretion ATP-binding protein|POSITIVE:POSITIVE|[1591342,1592094]|753 HSP 1 e-value: 1.0E-122 bit: 440.0 Len: 222 Query Start:1 Query End:222 Subject Strand: POSITIVE Subject Start: 1 Subject End: 222 taaattaaaattacccaagaaatctgtttataattgtttttatctttcgaattattattaattgtagtcggggctacaatattcatttagtagatataactttggaaagattgaaagctccctgtttatttttgtgttgggggataaatgaaaacacttaagcaaatttacagtgggtgggaatcgtagctgtaagaaatgggattattactggtacagtta................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... taaattaaaattacccaagaaatctgtttataattgtttttatctttcgaattattattaattgtagtcggggctacaatattcatttagtagatataactttggaaagattgaaagctccctgtttatttttgtgttgggggataaatgaaaacacttaagcaaatttacagtgggtgggaatcgtagctgtaagaaatgggattattactggtacagtta................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUUAAAAUUACCCAAGAAAUCUGUUUAUAAUUGUUUUUAUCUUUCGAAUUAUUAUUAAUUGUAGUCGGGGCUACAAUAUUCAUUUAGUAGAUAUAACUUUGGAAAGAUUGAAAGCUCCCUGUUUAUUUUUGUGUUGGGGGAUAAAUGAAAACACUUAAGCAAAUUUACAGUGGGUGGGAAUCGUAGCUGUAAGAAAUGGGAUUAUUACUGGUACAGUUA ..........(((((((..((((.((((((....((((((((((((((((..........((((((((....)))))))).((((...(((.......))).))))....)))))))(((((....(((....)))..)))))....)))))))))..)))))).))))....)))))))......((((((((.....(((........))).)))))))) (-50.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACUGUACCAGUAAUAAUCCCAUUUCUUACAGCUACGAUUCCCACCCACUGUAAAUUUGCUUAAGUGUUUUCAUUUAUCCCCCAACACAAAAAUAAACAGGGAGCUUUCAAUCUUUCCAAAGUUAUAUCUACUAAAUGAAUAUUGUAGCCCCGACUACAAUUAAUAAUAAUUCGAAAGAUAAAAACAAUUAUAAACAGAUUUCUUGGGUAAUUUUAAUUUA ...(((((..((.(((......))).)))))))...........(((((..(.(((((((.(((..((((((......((((..................)))).......(((((((...((((((.((........))..(((((((......))))))).....)))))).))))))).))))))....))).)))))))).)))))............ (-33.17) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 331 180 134 70 17 aact:AA01526|5end_amino_acid_ABC_transporter,_periplasmic_amino_acid-binding_protein_1024983..1025213_POSITIVE 314 205 162 64 19 aact:AA01357|5end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919133..919363_POSITIVE 307 214 171 85 46 aact:AA01449|5end_prophage_CP4-57_integrase_970569..970799_POSITIVE 305 220 176 105 52 aact:AA01957|5end_conserved_hypothetical_protein_1323211..1323441_POSITIVE 302 205 163 217 181 aact:AA01046|5end_conserved_hypothetical_protein_728862..729092_POSITIVE 299 217 173 70 17 aact:AA01349|5end_NAD(P)H_nitroreductase/H-flavin_oxidoreductase_913619..913849_POSITIVE 293 216 171 214 160 aact:AA02604|5end_thioredoxin_reductase_1815552..1815782_POSITIVE 283 219 175 49 6 aact:AA02226|5end_ABC_transporter_ATP-binding_protein_1514502..1514732_POSITIVE 275 218 171 176 121 aact:AA01770|5end_hypothetical_protein_1193111..1193341_POSITIVE 275 205 162 203 147 aact:AA00866|5end_conserved_hypothetical_protein_599925..600155_POSITIVE 269 219 174 126 76 aact:AA01266|5end_hypothetical_protein_862076..862306_POSITIVE 267 214 172 86 35 aact:AA02265|5end_exodeoxyribonuclease_V,_alpha_chain_1539701..1539931_POSITIVE 266 204 161 212 160 aact:AA02245|5end_hypothetical_protein_1527446..1527676_POSITIVE 266 197 151 160 117 aact:AA01119|5end_hypothetical_protein_769291..769521_POSITIVE 264 204 163 58 14 aact:AA01248|5end_conserved_hypothetical_protein_(possible_phosphoesterase)_849734..849964_POSITIVE 259 203 170 67 34 aact:AA00504|5end_ATPase_349909..350139_POSITIVE 257 204 161 160 116 aact:AA01564|5end_transaldolase_B_1049099..1049329_POSITIVE 253 204 162 205 166 aact:AA00039|5end_cytochrome_c-type_biogenesis_protein_30312..30542_POSITIVE 252 204 170 60 26 aact:AA00688|5end_curved_DNA-binding_protein_469165..469395_POSITIVE 250 203 162 60 22 aact:AA00423|5end_GTP-binding_protein_286533..286763_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 331 180 134 86 33 aact:AA01525|3end_amino_acid_ABC_transporter,_permease_component_1025079..1025229_POSITIVE 305 220 176 100 47 aact:AA01956|3end_3-deoxy-manno-octulosonate_cytidylyltransferase_1323286..1323436_POSITIVE 303 179 137 139 101 aact:AA01248|3end_conserved_hypothetical_protein_(possible_phosphoesterase)_850302..850452_POSITIVE 299 217 173 70 17 aact:AA01348|3end_hypothetical_protein_913699..913849_POSITIVE 293 216 171 151 97 aact:AA02603|3end_possible_thioredoxin_trxA_homolog_1815569..1815719_POSITIVE 283 219 175 59 16 aact:AA02225|3end_ABC_transporter_ATP-binding_protein_1514592..1514742_POSITIVE 275 218 171 117 62 aact:AA01769|3end_hypothetical_protein_1193132..1193282_POSITIVE 269 219 174 142 92 aact:AA01265|3end_hypothetical_protein_862172..862322_POSITIVE 267 214 172 70 19 aact:AA02263|3end_conserved_hypothetical_protein_1539765..1539915_POSITIVE 266 204 161 61 9 aact:AA02244|3end_conserved_hypothetical_protein_(probable_DNA-binding_protein;_death-on-curing_family_protein0_1527375..1527525_POSITIVE 261 216 173 87 28 aact:AA02844|3end_Holliday_junction_DNA_helicase_1997885..1998035_POSITIVE 259 163 121 140 102 aact:AA00015|3end_malate_dehydrogenase_8333..8483_POSITIVE 243 218 173 134 91 aact:AA02666|3end_DNA_helicase_II_1856583..1856733_POSITIVE 239 214 175 109 53 aact:AA02317|3end_conserved_hypothetical_protein_1583887..1584037_POSITIVE 239 177 133 121 74 aact:AA00660|3end_hypothetical_protein_453335..453485_POSITIVE 238 190 147 131 90 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 236 204 171 34 1 aact:AA00686|3end_hypothetical_protein_469219..469369_POSITIVE 235 124 81 144 90 aact:AA00700|3end_iron(III)_ABC_transporter,_ATP-binding_protein_481458..481608_POSITIVE 232 202 164 122 79 aact:AA02430|3end_conserved_hypothetical_protein_1675669..1675819_POSITIVE 232 213 173 149 111 aact:AA02143|3end_hypothetical_protein_1467641..1467791_POSITIVE