Origin IGS: tttttacagagttttttcttctcctgccaaggaaattttctcttcatccgatcagagacgcattctcaaattaaagaaagcgtgttataatctcccgcaaaattggtaggcagggtcatagccaacctaaaccttttcgtttaacttaaaaataataaggtgaaatctt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aagatttcaccttattatttttaagttaaacgaaaaggtttaggttggctatgaccctgcctaccaattttgcgggagattataacacgctttctttaatttgagaatgcgtctctgatcggatgaagagaaaatttccttggcaggagaagaaaaaactctgtaaaaa Mask Tandem Repeat Region ================================================ tttttacagagttttttcttctcctgccaaggaaattttctcttcatccgatcagagacgcattctcaaattaaagaaagcgtgttataatctcccgcaaaattggtaggcagggtcatagccaacctaaaccttttcgtttaacttaaaaataataaggtgaaatctt Find is-nt database================================================ Query_seq: AA02307:AA02309|AA02307:AA02309:5'-guanylate kinase:glyceraldehyde 3-phosphate dehydrogenase:->->:1578119..1578287 169 tttttacagagttttttcttctcctgccaaggaaattttctcttcatccgatcagagacgcattctcaaattaaagaaagcgtgttataatctcccgcaaaattggtaggcagggtcatagccaacctaaaccttttcgtttaacttaaaaataataaggtgaaatctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA02307:AA02309|AA02307:AA02309:5'-guanylate kinase:glyceraldehyde 3-phosphate dehydrogenase:->->:1578119..1578287 169 tttttacagagttttttcttctcctgccaaggaaattttctcttcatccgatcagagacgcattctcaaattaaagaaagcgtgttataatctcccgcaaaattggtaggcagggtcatagccaacctaaaccttttcgtttaacttaaaaataataaggtgaaatctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA02307:AA02309|AA02307:AA02309:5'-guanylate kinase:glyceraldehyde 3-phosphate dehydrogenase:->->:1578119..1578287 169 tttttacagagttttttcttctcctgccaaggaaattttctcttcatccgatcagagacgcattctcaaattaaagaaagcgtgttataatctcccgcaaaattggtaggcagggtcatagccaacctaaaccttttcgtttaacttaaaaataataaggtgaaatctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttttacagagttttttcttctcctgccaaggaaattttctcttcatccgatcagagacgcattctcaaattaaagaaagcgtgttataatctcccgcaaaattggtaggcagggtcatagccaacctaaaccttttcgtttaacttaaaaataataaggtgaaatctt Predict ORF larger than 30AA ================================================ tttttacagagttttttcttctcctgccaaggaaattttctcttcatccgatcagagacgcattctcaaattaaagaaagcgtgttataatctcccgcaaaattggtaggcagggtcatagccaacctaaaccttttcgtttaacttaaaaataataaggtgaaatctt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 87 Start: 62 End: 91 .............................................................ATTCTCAAATTAAAGAAAGCGTGTTATAAT.............................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttttacagagttttttcttctcctgccaaggaaattttctcttcatccgatcagagacgcattctcaaattaaagaaagcgtgttataatctcccgcaaaattggtaggcagggtcatagccaacctaaaccttttcgtttaacttaaaaataataaggtgaaatctt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA02307:AA02309|AA02307:AA02309:5'-guanylate kinase:glyceraldehyde 3-phosphate dehydrogenase:->->:1578119..1578287 169 tttttacagagttttttcttctcctgccaaggaaattttctcttcatccgatcagagacgcattctcaaattaaagaaagcgtgttataatctcccgcaaaattggtaggcagggtcatagccaacctaaaccttttcgtttaacttaaaaataataaggtgaaatctt Intra-Species Hit: Count: 1 Min: 1 Max: 169 Len: 169 Subject: aact_AA02307_AA02309|5'-guanylate kinase:glyceraldehyde 3-phosphate dehydrogenase|POSITIVE:POSITIVE|[1578119,1578287]|169 HSP 1 e-value: 3.0E-91 bit: 335.0 Len: 169 Query Start:1 Query End:169 Subject Strand: POSITIVE Subject Start: 1 Subject End: 169 tttttacagagttttttcttctcctgccaaggaaattttctcttcatccgatcagagacgcattctcaaattaaagaaagcgtgttataatctcccgcaaaattggtaggcagggtcatagccaacctaaaccttttcgtttaacttaaaaataataaggtgaaatctt tttttacagagttttttcttctcctgccaaggaaattttctcttcatccgatcagagacgcattctcaaattaaagaaagcgtgttataatctcccgcaaaattggtaggcagggtcatagccaacctaaaccttttcgtttaacttaaaaataataaggtgaaatctt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUUACAGAGUUUUUUCUUCUCCUGCCAAGGAAAUUUUCUCUUCAUCCGAUCAGAGACGCAUUCUCAAAUUAAAGAAAGCGUGUUAUAAUCUCCCGCAAAAUUGGUAGGCAGGGUCAUAGCCAACCUAAACCUUUUCGUUUAACUUAAAAAUAAUAAGGUGAAAUCUU .((((((.(.(((....(((..((((((((.(((........)))....(((..((.((((.((((........)))).)))).))...)))..........))))))))..)))....))))....(((((......)))))................)))))).... (-29.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGAUUUCACCUUAUUAUUUUUAAGUUAAACGAAAAGGUUUAGGUUGGCUAUGACCCUGCCUACCAAUUUUGCGGGAGAUUAUAACACGCUUUCUUUAAUUUGAGAAUGCGUCUCUGAUCGGAUGAAGAGAAAAUUUCCUUGGCAGGAGAAGAAAAAACUCUGUAAAAA ....((((......((((..((((.((((((......)))))).))))..)))).((((((............((((((((.....((((.(((((......))))).))))((((..........))))..)))))))).))))))....)))).............. (-37.62) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 354 139 93 68 10 aact:AA01875|5end_PTS_system,_glucose-specific_enzyme_II,_A_component_1262563..1262793_POSITIVE 311 123 95 55 16 aact:AA02604|5end_thioredoxin_reductase_1815552..1815782_POSITIVE 295 69 24 152 108 aact:AA01947|5end_iron(III)-transport_ATP-binding_protein_1316390..1316620_POSITIVE 294 133 93 227 192 aact:AA01561|5end_conserved_hypothetical_protein_1046505..1046735_POSITIVE 290 133 92 201 155 aact:AA00895|5end_hypothetical_protein_621285..621515_POSITIVE 289 139 95 78 37 aact:AA01598|5end_conserved_hypothetical_protein_1078954..1079184_POSITIVE 286 129 83 209 170 aact:AA01188|5end_conserved_hypothetical_protein_813912..814142_POSITIVE 284 128 82 159 114 aact:AA02851|5end_hypothetical_protein_2000896..2001126_POSITIVE 284 127 93 89 47 aact:AA01011|5end_alanyl-tRNA_synthetase_(alanine--tRNA_ligase)_705149..705379_POSITIVE 282 130 91 195 162 aact:AA01779|5end_molybdenum_ABC_transporter,_ATP-binding_protein_1200036..1200266_POSITIVE 276 115 80 217 180 aact:AA02507|5end_penicillin-insensitive_murein_endopeptidase_A_1740058..1740288_POSITIVE 265 127 81 215 164 aact:AA02813|5end_hypothetical_protein_1980045..1980275_POSITIVE 263 128 91 80 45 aact:AA02966|5end_conserved_hypothetical_protein_2074223..2074453_POSITIVE 263 122 81 118 75 aact:AA00525|5end_hypothetical_protein_359254..359484_POSITIVE 260 140 91 185 126 aact:AA01418|5end_conserved_hypothetical_protein_953951..954181_POSITIVE 259 133 97 201 165 aact:AA00962|5end_exopolyphosphatase_(metaphosphatase)_671060..671290_POSITIVE 259 115 79 192 158 aact:AA00042|5end_hypothetical_protein_33810..34040_POSITIVE 256 118 79 227 196 aact:AA02673|5end_gluconate_utilization_operon_repressor_1858913..1859143_POSITIVE 256 119 78 91 37 aact:AA02067|5end_conserved_hypothetical_protein;_possible_membrane_protein_1405233..1405463_POSITIVE 256 128 82 222 180 aact:AA01316|5end_hypothetical_protein_891262..891492_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 304 122 81 48 1 aact:AA01421|3end_phosphoglycolate_phosphatase_955806..955956_POSITIVE 294 133 93 147 112 aact:AA01559|3end_RecA_regulatory_protein,_RecX_1046505..1046655_POSITIVE 293 139 91 110 56 aact:AA02947|3end_riboflavin_biosynthesis_protein_2061874..2062024_POSITIVE 284 127 93 113 71 aact:AA01010|3end_hypothetical_protein_705253..705403_POSITIVE 282 130 91 44 11 aact:AA01778|3end_aminopeptidase_A/I;_cytosol_aminopeptidase;_leucyl_aminopeptidase_1199965..1200115_POSITIVE 279 119 73 123 82 aact:AA00172|3end_conserved_hypothetical_protein_(possible_regulator_protein_/_nitrogen_fixation_protein)_122904..123054_POSITIVE 275 118 75 75 35 aact:AA01167|3end_glucose-6-phosphate_1-dehydrogenase_801067..801217_POSITIVE 268 127 91 150 119 aact:AA00377|3end_acetyl-CoA_acetyltransferase_(Beta-ketothiolase,3-ketoacyl-CoA_thiolase)_254300..254450_POSITIVE 263 128 91 63 28 aact:AA02965|3end_hypothetical_protein_2074286..2074436_POSITIVE 259 115 79 119 85 aact:AA00041|3end_cytochrome_c-type_biogenesis_protein_33817..33967_POSITIVE 256 118 79 151 120 aact:AA02671|3end_gluconokinase_1858917..1859067_POSITIVE 256 119 78 92 38 aact:AA02066|3end_hypothetical_protein_1405314..1405464_POSITIVE 256 129 83 72 38 aact:AA00978|3end_conserved_hypothetical_protein_684585..684735_POSITIVE 252 127 87 74 35 aact:AA00160|3end_pilA-like_biogenesis_protein_/_prepilin_peptidase_dependent_proteinD_113434..113584_POSITIVE 250 140 96 68 29 aact:AA01840|3end_conserved_hypothetical_protein_1237554..1237704_POSITIVE 249 127 81 47 4 aact:AA02844|3end_Holliday_junction_DNA_helicase_1997885..1998035_POSITIVE 249 118 71 142 83 aact:AA00551|3end_hypothetical_protein_377403..377553_POSITIVE 246 124 81 142 97 aact:AA01972|3end_phosphotransacetylase_1334431..1334581_POSITIVE 245 86 45 73 23 aact:AA02251|3end_biotin_sulfoxide_reductase;_Trimethylamine-N-oxide_reductase_precursor_1533843..1533993_POSITIVE 245 128 88 103 67 aact:AA01634|3end_hypothetical_protein_1104670..1104820_POSITIVE